Marine and Freshwater Research Marine and Freshwater Research Society
Advances in the aquatic sciences
RESEARCH ARTICLE

Larval anisakid nematodes in teleost fishes from Lizard Island, northern Great Barrier Reef, Australia

Abdul Jabbar A F , Abdouslam Asnoussi B , Luke J. Norbury B , Albert Eisenbarth B , Shokoofeh Shamsi C , Robin B. Gasser A , Andreas L. Lopata B D E and Ian Beveridge A

A Faculty of Veterinary Science, The University of Melbourne, Werribee, Vic. 3030, Australia.

B School of Applied Science, RMIT University, Bundoora, Vic. 3083, Australia.

C School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.

D School of Pharmacy and Molecular Sciences, James Cook University, Townsville, Qld 4811, Australia.

E Centre for Biodiscovery and Molecular Development of Therapeutics, Qld 4878, Australia.

F Corresponding author. Email: jabbara@unimelb.edu.au

Marine and Freshwater Research 63(12) 1283-1299 http://dx.doi.org/10.1071/MF12211
Submitted: 7 August 2012  Accepted: 12 October 2012   Published: 12 December 2012

Abstract

A survey was undertaken to characterise larval anisakid nematodes present in teleosts at Lizard Island on the northern Great Barrier Reef. In total, 464 fish were examined from 32 families, 62 genera and 107 species. Anisakid nematodes were found in 46 (9.9%) of the fish examined. Infections in Atherinidae, Lethrinidae, Lutjanidae and Serranidae were moderately prevalent, with the intensities of infection ranging from 1 to 80; whereas in the Sphyraenidae and Scombridae, the prevalence of infection was very high, with intensities ranging from 1 to >375 anisakids. A combined morphological and molecular-phylogenetic approach was employed to identify larval anisakid nematodes to species and/or genotypes. The nematodes examined were identified as Anisakis typica (three genotypes based on molecular characterisation), Terranova Types I (five genotypes) and II (five genotypes) and Hysterothylacium Types IV, V (four genotypes), VI and X. The findings of the present study provide some insights into the distribution of larval anisakid nematodes in coral-reef fishes and a basis for future investigations of anisakid populations in marine fishes.

Additional keywords: Anisakidae, first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA, Nematoda, single-strand conformation polymorphism (SSCP) analysis.


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