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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Evaluating the potential of a novel dual heat-pulse sensor to measure volumetric water use in grapevines under a range of flow conditions

Kyle R. Pearsall A , Larry E. Williams A B , Sean Castorani A C , Tim M. Bleby D E and Andrew J. McElrone A C F
+ Author Affiliations
- Author Affiliations

A Department of Viticulture and Enology, University of California – Davis, Davis, CA 95616, USA.

B Kearney Agricultural Research and Extension Center, 9240 S. Riverbend Avenue, Parlier, CA 93648, USA.

C USDA-ARS, Davis, CA 95616, USA.

D School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia.

E Astron Environmental Services, 129 Royal Street, East Perth, WA 6004, Australia.

F Corresponding author. Email: ajmcelrone@ucdavis.edu

Functional Plant Biology 41(8) 874-883 https://doi.org/10.1071/FP13156
Submitted: 23 May 2013  Accepted: 14 February 2014   Published: 22 April 2014

Abstract

The aim of this study was to validate a novel, dual sap-flow sensor that combines two heat-pulse techniques in a single set of sensor probes to measure volumetric water use over the full range of sap flows found in grapevines. The heat ratio method (HRM), which works well at measuring low and reverse flows, was combined with the compensation heat-pulse method (CHPM) that captures moderate to high flows. Sap-flow measurements were performed on Vitis vinifera L. (cvv. Thompson seedless, Chardonnay and Cabernet Sauvignon) grapevines growing in a greenhouse and in three different vineyards, one of which contained a field weighing lysimeter. The combined heat-pulse techniques closely tracked diurnal grapevine water use determined through lysimetry in two growing seasons, and this was true even at very high flow rates (>6 L vine–1 h–1 for Thompson seedless vines in the weighing lysimeter). Measurements made with the HRM technique under low flow conditions were highly correlated (R2 ~ 0.90) with those calculated using the compensated average gradient method that is used to resolve low flow with the CHPM method. Volumetric water use determined with the dual heat-pulse sensors was highly correlated with hourly lysimeter water use in both years (R2 = 0.92 and 0.94 in 2008 and 2009 respectively), but the nature of the relationship was inconsistent among replicate sensors. Similar results were obtained when comparing grapevine water use determined from sap-flow sensors to miniaturised weighing lysimetry of 2-year-old potted vines and to meteorological estimates for field-grown vines in two additional vineyards. The robust nature of all of the correlations demonstrates that the dual heat-pulse sensors can be used to effectively track relative changes in plant water use, but variability of flow around stems makes it difficult to accurately convert to sap-flow volumes.

Additional keywords: heat pulse velocity, sap flow, Vitis vinifera, weighing lysimetry.


References

Allen RG, Pereira LS, Raes D, Smith M (1998) Crop evapotranspiration: guidelines for computing crop water requirements. FAO Irrigation and Drainage Paper 56. FAO, Rome.

Bleby TM, Burgess SSO, Adams MA (2004) A validation, comparison and error analysis of two heat-pulse methods for measuring sap flow in Eucalyptus marginata saplings. Functional Plant Biology 31, 645–658.
A validation, comparison and error analysis of two heat-pulse methods for measuring sap flow in Eucalyptus marginata saplings.Crossref | GoogleScholarGoogle Scholar |

Bleby TM, McElrone AJ, Burgess SSO (2008) Limitations of the HRM: great at low flow rates, but no yet up to speed? In ‘7th International Workshop on Sap Flow: Book of Abstracts’. (International Society of Horticultural Sciences: Seville, Spain)

Bleby TM, McElrone AJ, Jackson RB (2010) Water uptake and hydraulic redistribution across large woody root systems to 20 m depth. Plant, Cell & Environment 33, 2132–2148.
Water uptake and hydraulic redistribution across large woody root systems to 20 m depth.Crossref | GoogleScholarGoogle Scholar |

Braun P, Schmid J (1999) Sap-flow measurements in grapevines (Vitis vinifera L.) 1. Stem morphology and use of the heat balance method. Plant and Soil 215, 39–45.
Sap-flow measurements in grapevines (Vitis vinifera L.) 1. Stem morphology and use of the heat balance method.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXos1anuw%3D%3D&md5=5c21d010a8970579a3fe98dd7211801cCAS |

Burgess SSO, Adams MA, Bleby TM (2000) Measurement of sap flow in roots of woody plants: a commentary. Tree Physiology 20, 909–913.
Measurement of sap flow in roots of woody plants: a commentary.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3MvgvFWnsA%3D%3D&md5=328180dfb7814da0f2ad1b349da04705CAS |

Burgess SSO, Adams MA, Turner NC, Beverly CR, Ong CK, Khan AAH, Bleby TM (2001) An improved heat-pulse method to measure low and reverse rates of sap flow in woody plants. Tree Physiology 21, 589–598.
An improved heat-pulse method to measure low and reverse rates of sap flow in woody plants.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD38%2Fht1Kjsw%3D%3D&md5=cdc826baa219c81c7943395324d9e0b2CAS |

Collins MJ, Fuentes S, Barlow EWR (2010) Partial rootzone drying and deficit irrigation increase stomatal sensitivity to vapour pressure deficit in anisohydric grapevines. Functional Plant Biology 37, 128–138.
Partial rootzone drying and deficit irrigation increase stomatal sensitivity to vapour pressure deficit in anisohydric grapevines.Crossref | GoogleScholarGoogle Scholar |

Dye PJ, Olbrich BW, Poulter AG (1991) The influence of growth rings in Pinus patula on heat pulse velocity and sap flow measurement. J. Exp. Bot. 42, 867–870.
The influence of growth rings in Pinus patula on heat pulse velocity and sap flow measurement.Crossref | GoogleScholarGoogle Scholar |

Eastham J, Gray SA (1998) A preliminary evaluation of the suitability of sap-flow sensors for use in scheduling vineyard irrigation. American Journal of Enology and Viticulture 49, 171–176.

Fernández JE, Green SR, Caspari HW, Diaz-Espejo A, Cuevas MV (2008) The use of sap-flow measurements for scheduling irrigation in olive, apple and Asian pear trees and in grapevines. Plant and Soil 305, 91–104.
The use of sap-flow measurements for scheduling irrigation in olive, apple and Asian pear trees and in grapevines.Crossref | GoogleScholarGoogle Scholar |

Ginestar C, Eastham J, Gray S, Iland P (1998a) Use of sap-flow sensors to schedule vineyard irrigation. I. Effects of post-verasion water deficits on water relations, vine growth, and yield of Shiraz grapevines. American Journal of Enology and Viticulture 49, 413–420.

Ginestar C, Eastham J, Gray S, Iland P (1998b) Use of sap-flow sensors to schedule vineyard irrigation. II. Effects of post-verasion water deficits on composition of Shiraz grapes. American Journal of Enology and Viticulture 49, 421–428.

Green SR, Clothier BE (1988) Water use of kiwifruit vines and apple trees by the heat-pulse technique. Journal of Experimental Botany 39, 115–123.
Water use of kiwifruit vines and apple trees by the heat-pulse technique.Crossref | GoogleScholarGoogle Scholar |

Green SR, Romero R (2012) Can we improve heat-pulse to measure low and reverse flows? Acta Horticulturae 951, 19–29.

Green SR, Clothier B, Jardine B (2003) Theory and practical application of heat pulse to measure sap flow. Agronomy Journal 95, 1371–1379.
Theory and practical application of heat pulse to measure sap flow.Crossref | GoogleScholarGoogle Scholar |

Intrigliolo DS, Lakso AN, Piccioni RM (2009) Grapevine cv. ‘Riesling’ water use in the northeastern United States. Irrigation Science 27, 253–262.
Grapevine cv. ‘Riesling’ water use in the northeastern United States.Crossref | GoogleScholarGoogle Scholar |

Lascano RJ, Baumhardt RL, Lipe WN (1992) Measurement of water flow in young grapevines using the stem heat balance method. American Journal of Enology and Viticulture 43, 159–165.

Lu P, Muller WJ, Chacko EK (2000) Spatial variations in xylem sap-flux density in the trunk of orchard-grown, mature mango trees under changing soil water conditions. Tree Physiology 20, 683–692.
Spatial variations in xylem sap-flux density in the trunk of orchard-grown, mature mango trees under changing soil water conditions.Crossref | GoogleScholarGoogle Scholar | 12651518PubMed |

Lu P, Yunusa IAM, Walker RR, Muller WJ (2003) Regulation of canopy conductance and transpiration and their modeling in irrigated grapevines. Functional Plant Biology 30, 689–698.
Regulation of canopy conductance and transpiration and their modeling in irrigated grapevines.Crossref | GoogleScholarGoogle Scholar |

Marshall DC (1958) Measurement of sap flow in conifers by heat transport. Plant Physiology 33, 385–396.
Measurement of sap flow in conifers by heat transport.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28zhtleltQ%3D%3D&md5=3301250c64692db752b793e839b31cd5CAS | 16655154PubMed |

Patakas A, Noitsakis B, Chouzouri A (2005) Optimization of irrigation water use in grapevines using the relationship between transpiration and plant water use. Agriculture, Ecosystems & Environment 106, 253–259.
Optimization of irrigation water use in grapevines using the relationship between transpiration and plant water use.Crossref | GoogleScholarGoogle Scholar |

Petrie PR, Trought MCT, Howell GS, Buchan GD, Palmer JW (2009) Whole-canopy gas exchange and light interception of vertically trained Vitis vinifera L. under direct and diffuse light. American Journal of Enology and Viticulture 60, 173–182.

Romero R, Muriel JL, Garcia I, Green SR, Clothier BE (2012) Improving heat-pulse methods to extend the measurement range including reverse flows. Acta Horticulturae 951, 31–38.

Sano Y, Okamura Y, Utsumi Y (2005) Visualizing water-conduction pathways of living trees: selection of dyes and tissue preparation methods. Tree Physiology 25, 269–275.
Visualizing water-conduction pathways of living trees: selection of dyes and tissue preparation methods.Crossref | GoogleScholarGoogle Scholar | 15631975PubMed |

Shackel KA, Johnson RS, Medawar CK (1992) Substantial error in estimates of sap flow using the heat balance technique on woody stems under field conditions. Journal of the American Society for Horticultural Science 117, 351–356.

Swanson RH (1983) ‘Numerical and experimental analysis of implanted-probe heat-pulse velocity theory.’ PhD thesis. University of Alberta, Edmonton, Canada.

Tarara JM, Ferguson JC (2001) Device for simulating high rates of sap flow in grapevines. American Journal of Enology and Viticulture 52, 260–265.

Testi L, Villalobos F (2009) New approach for measuring low sap velocities in trees. Agricultural Meteorology 149, 730–734.
New approach for measuring low sap velocities in trees.Crossref | GoogleScholarGoogle Scholar |

Vandegehuchte MW, Steppe K (2012) Improving sap-flux density measurements by correctly determining thermal diffusivity, differentiating between bound and unbound water. Tree Physiology 32, 930–942.

Vandegehuchte MW, Steppe K (2013) Sap-flux density measurement methods: working principles and applicability. Functional Plant Biology 40, 213–223.
Sap-flux density measurement methods: working principles and applicability.Crossref | GoogleScholarGoogle Scholar |

Vertessy RA, Hatton TJ, Reece P, O’Sullivan SK, Benyon RG (1997) Estimating stand water use of large mountain ash trees and validation of the sap-flow measurement technique. Tree Physiology 17, 747–756.
Estimating stand water use of large mountain ash trees and validation of the sap-flow measurement technique.Crossref | GoogleScholarGoogle Scholar | 14759884PubMed |

Williams LE (2010) Interaction of rootstock and applied water amounts at various fractions of estimated evapotranspiration (ET c) on productivity of Cabernet Sauvignon. Australian Journal of Grape and Wine Research 16, 434–444.
Interaction of rootstock and applied water amounts at various fractions of estimated evapotranspiration (ET c) on productivity of Cabernet Sauvignon.Crossref | GoogleScholarGoogle Scholar |

Williams LE, Araujo F (2002) Correlations among predawn leaf, midday leaf, and midday stem water potential and their correlations with other measures of soil and plant water status in Vitis vinifera L. Journal of the American Society for Horticultural Science 127, 448–454.

Williams LE, Ayars JE (2005a) Water use of Thompson seedless grapevines as affected by the application of gibberellic acid (GA3) and trunk girdling – practices to increase berry size. Agricultural and Forest Meteorology 129, 85–94.
Water use of Thompson seedless grapevines as affected by the application of gibberellic acid (GA3) and trunk girdling – practices to increase berry size.Crossref | GoogleScholarGoogle Scholar |

Williams LE, Ayars JE (2005b) Grapevine water use and the crop coefficient are linear functions of the shaded area measured beneath the canopy. Agricultural and Forest Meteorology 132, 201–211.
Grapevine water use and the crop coefficient are linear functions of the shaded area measured beneath the canopy.Crossref | GoogleScholarGoogle Scholar |

Williams LE, Baeza P (2007) Relationships among ambient temperature and vapor pressure deficit and leaf and stem water potentials of fully irrigated, field-grown grapevines. American Journal of Enology and Viticulture 58, 173–181.

Williams LE, Phene CJ, Grimes DW, Trout TJ (2003a) Water use of young Thompson seedless grapevines in California. Irrigation Science 22, 1–9.

Williams LE, Phene CJ, Grimes DW, Trout TJ (2003b) Water use of mature Thompson seedless grapevines in California. Irrigation Science 22, 11–18.

Williams LE, Grimes DW, Phene CJ (2010) The effects of applied water at various fraction of measured evapotranspiration on reproductive growth and water productivity of Thompson seedless. Irrigation Science 28, 221–232.
The effects of applied water at various fraction of measured evapotranspiration on reproductive growth and water productivity of Thompson seedless.Crossref | GoogleScholarGoogle Scholar |

Williams LE, Baeza P, Vaughn P (2011) Midday measurements of leaf water potential and stomatal conductance are highly correlated with daily water use of Thompson seedless grapevines measured with a weighing lysimeter during two dry-down periods. Irrigation Science 30, 201–212.
Midday measurements of leaf water potential and stomatal conductance are highly correlated with daily water use of Thompson seedless grapevines measured with a weighing lysimeter during two dry-down periods.Crossref | GoogleScholarGoogle Scholar |

Yunusa IAM, Walker RR, Loveys BR, Blackmore DH (2000) Determination of transpiration in irrigated grapevines: comparison of the heat-pulse technique with gravimetric and micrometeorological methods. Irrigation Science 20, 1–8.
Determination of transpiration in irrigated grapevines: comparison of the heat-pulse technique with gravimetric and micrometeorological methods.Crossref | GoogleScholarGoogle Scholar |