CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
blank image Search
blank image blank image
blank image
  Advanced Search

Journal Home
About the Journal
Editorial Structure
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Evolutionary Reviews
Sample Issue
Call for Papers
For Authors
General Information
Submit Article
Author Instructions
Open Access
Awards and Prizes
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter LinkedIn

red arrow PrometheusWiki
blank image
Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 41(8)

SsHKT1;1 is a potassium transporter of the C3 halophyte Suaeda salsa that is involved in salt tolerance

Qun Shao A, Ning Han A C, Tonglou Ding A, Feng Zhou A C and Baoshan Wang A D

A Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan 250?014, China.
B Present address: Key Laboratory of Biotechnology, College of Food and Biologic Engineering, Qi Lu University of Technology, Jinan 250?353, China.
C Present address: School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211?171, China.
D Corresponding author. Email: bswang@sdnu.edu.cn

Functional Plant Biology 41(8) 790-802 http://dx.doi.org/10.1071/FP13265
Submitted: 5 September 2013  Accepted: 28 January 2014   Published: 28 April 2014

PDF (1.1 MB) $25
 Supplementary Material
 Export Citation

SsHKT1;1, a HKT1 homologue, was isolated from the C3 halophyte Suaeda salsa L. and its ion transport properties were investigated in heterologous systems. The expression of SsHKT1;1 suppressed a K+ transport-defective phenotype of the yeast strain CY162 (Δtrk1Δtrk2), suggesting the enhancement of K+ uptake with SsHKT1;1. However, it did not suppress the salt-sensitive phenotype of the yeast strain G19 (Δena14), which lacks a major component of Na+ efflux. Transgenic Arabidopsis thaliana (L.) Heynh. plants overexpressing SsHKT1;1 showed enhanced salt tolerance and increased shoot K+ concentration, whereas no significant changes in shoot Na+ concentration were observed. S. salsa was also used to investigate K+ uptake properties under salinity. The K+ transporters in the roots selectively mediated K+ uptake irrespective of external Na+ and their inhibitor did not affect Na+ uptake at low K+. Thus, both molecular and physiological studies provide strong in vivo evidence that SsHKT1;1 mainly acts as a potassium transporter in heterologous expression systems and S. salsa, and that it is involved in salt tolerance by taking part in the maintenance of cytosolic cation homeostasis, particularly, in the maintenance of K+ nutrition under salinity.

Additional keywords: euhalophyte, heterologous systems, ion transport properties.


Ali Z, Park HC, Ali A, Oh DH, Aman R, Kropornicka A, Hong H, Choi W, Chung WS, Kim WY, Bressan RA, Bohnert HJ, Lee SY, Yun DJ (2012) TsHKT1;2, a HKT1 homolog from the extremophile Arabidopsis relative Thellungiella salsuginea, shows K+ specificity in the presence of NaCl. Plant Physiology 158, 1463–1474.
CrossRef | CAS | PubMed |

Anderson JA, Huprikar SS, Kochian LV, Lucas WJ, Gaber RF (1992) Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America 89, 3736–3740.
CrossRef | CAS | PubMed |

Asins MJ, Villalta I, Aly MM, Olias R, De Morals PA, Huertas R, Li J, Jaime-Perez N, Haro R, Raga V (2013) Two closely linked tomato HKT coding genes are positional candiates for the major tomato QTL involved in Na+/K+ homeostasis. Plant, Cell & Environment 36, 1171–1191.
CrossRef | CAS |

Bañuelos MA, Haro R, Fraile-Escanciano A, Rodríguez-Navarro A (2008) Effects of polylinker uATGs on the function of grass HKT1 transporters expressed in yeast cells. Plant & Cell Physiology 49, 1128–1132.
CrossRef |

Berthomieu P, Conejero G, Nublat A, Brackenbury WJ, Lambert C, Savio C, Uozumi N, Oiki S, Yamada K, Cellier F, Gosti F, Simonneau T, Essah PA, Tester M, Very AA, Sentenac H, Casse F (2003) Functional analysis of AtHKT1 in Arabidopsis shows that Na+ recirculation by the phloem is crucial for salt tolerance. The EMBO Journal 22, 2004–2014.
CrossRef | CAS | PubMed |

Davenport RJ, Munoz-Mayor A, Jha D (2007) The Na+ transporter AtHKT1 controls retrieval of Na+ from the xylem in Arabidopsis. Plant, Cell & Environment 30, 497–507.
CrossRef | CAS |

Fairbairn DJ, Liu W, Schachtman DP, Sara GG, Day SR, Teasdale RD (2000) Characterisation of two distinct HKT1-like potassium transporters from Eucalyptus camaldulensis. Plant Molecular Biology 43, 515–525.
CrossRef | CAS | PubMed |

Garciadeblás B, Senn ME, Baríuelos MA, Rodríguez-Navarro A (2003) Sodium transport and HKT transporters: the rice model. The Plant Journal 34, 788–801.
CrossRef | PubMed |

Haro R, Baríuelos MA, Senn ME, Barrero-Gil J, Rodríguez-Navarro A (2005) HKT1 mediates sodium uniport in roots. Pitfalls in the expression of HKT1 in yeast. Plant Physiology 139, 1495–1506.
CrossRef | CAS | PubMed |

Hauser F, Horie T (2010) A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress. Plant, Cell & Environment 33, 552–565.
CrossRef | CAS |

Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. California Agricultural Experiment Station Circular 347, 1–32.

Horie T, Horie R, Chan WY, Leung HY, Schroeder JI (2006) Calcium regulation of sodium hypersensitivities of sos3 and athkt1 mutants. Plant & Cell Physiology 47, 622–633.
CrossRef | CAS |

Horie T, Costa A, Kim TH, Han MJ, Horie R, Leung HY, Miyao A, Hirochika H, An G, Schroeder JI (2007) Rice OsHKT2;1 transporter mediates large Na+ influx component into K+-starved roots for growth. The EMBO Journal 26, 3003–3014.
CrossRef | CAS | PubMed |

Horie T, Brodsky DE, Costa A, Kaneko T, Schiavo FL, Katsuhara M, Schroeder JI (2011) K+ transport by the OsHKT2;4 transporter from rice with a typical Na+ transport properties and competition in permeation of K+ over Mg2+ and Ca2+ ions. Plant Physiology 156, 1493–1507.
CrossRef | CAS | PubMed |

Huang SB, Spielmeyer W, Lagudah ES, James RA, Platten JD, Dennis ES, Munns R (2006) A sodium transporter (HKT7) is a candidate for Nax1, a gene for salt tolerance in durum wheat. Plant Physiology 142, 1718–1727.
CrossRef | CAS |

James RA, Blake C, Byrt CS, Munns R (2011) Major genes for Na+ exclusion, Nax1 and Nax2 (wheat HKT1;4 and HKT1;5), decrease Na+ accumulation in bread wheat leaves under saline and waterlogged conditions. Journal of Experimental Botany 62, 2939–2947.
CrossRef | CAS | PubMed |

Kochian LV, Jiao XZ, Lucas WJ (1985) Potassium transport in corn roots. IV. Characterization of the linear component. Plant Physiology 79, 771–776.
CrossRef | CAS | PubMed |

Laurie S, Feeney KA, Maathuis FJM, Heard PJ, Brown SJ, Leigh RA (2002) A role for HKT1 in sodium uptake by wheat roots. The Plant Journal 32, 139–149.
CrossRef | CAS | PubMed |

Li PH, Chen M, Wang BS (2002) Effect of K+ nutrition on growth and activity of leaf tonoplast V-H+-ATPase and V-H+-PPase of Suaeda salsa under NaCl stress. Acta Botanica Sinica 44, 433–440.

Mäser P, Hosoo Y, Goshima S, Horie T, Eckelman B, Yamada K, Yoshida K, Bakker EP, Shinmyo A, Oiki S, Schroeder JI, Uozumi N (2002) Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants. Proceedings of the National Academy of Sciences of the United States of America 99, 6428–6433.
CrossRef | PubMed |

Møller IS, Gilliham M, Jha D, Mayo GM, Roy SJ, Coates JC, Haseloff J, Tester M (2009) Shoot Na+ exclusion and increased salinity tolerance engineered by cell type-specific alteration of Na+ transport in Arabidopsis. The Plant Cell 21, 2163–2178.
CrossRef | PubMed |

Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651–681.
CrossRef | CAS | PubMed |

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.

Platten JD, Cotsaftis O, Berthomieu P, Bohnert H, Davenport RJ, Fairbairn DJ, Horie T, Leigh RA, Lin HX, Luan S, Mäser P, Pantoja O, Rodríguez-Navarro A, Schachtman DP, Schroeder JI, Sentenac H, Uozumi N, Véry AA, Zhu JK, Dennis ES, Tester M (2006) Nomenclature for HKT transporters, key determinants of plant salinity tolerance. Trends in Plant Science 11, 372–374.
CrossRef | CAS | PubMed |

Plett D, Safwat G, Gilliham M, Skrumsager Møller I, Roy S, Shirley N, Jacobs A, Johnson A, Tester M (2010) Improved salinity tolerance of rice through cell type-specific expression of AtHKT1;1. PLoS ONE 5, e12571
CrossRef | PubMed |

Qiu L, Wu DZ, Ali S, Cai SG, Dai F, Jin XL, Wu FB, Zhang GP (2011) Evaluation of salinity tolerance and analysis of allelic function of HvHKT1 and HvHKT2 in Tibetan wild barley. Theoretical and Applied Genetics 122, 695–703.
CrossRef | CAS | PubMed |

Ren ZH, Gao JP, Li LG, Cai XL, Huang W, Chao DY, Zhu MZ, Wang ZY, Luan S, Lin HX (2005) A rice quantitative trait locus for salt tolerance encodes a sodium transporter. Nature Genetics 37, 1141–1146.
CrossRef | CAS | PubMed |

Rodríguez-Navarro A, Rubio F (2006) High-affinity potassium and sodium transport systems in plants. Journal of Experimental Botany 57, 1149–1160.
CrossRef | PubMed |

Rubio F, Schwarz M, Gassmann W, Schroeder JI (1999) Genetic selection of mutations in the high affinity K+ transporter HKT1 that define functions of a loop site for reduced Na+ permeability and increased Na+ tolerance. The Journal of Biological Chemistry 274, 6839–6847.
CrossRef | CAS | PubMed |

Rus A, Baxter I, Muthukumar B, Gustin J, Lahner B, Yakubova E, Salt DE (2006) Natural variants of AtHKT1 enhance Na+ accumulation in two wild populations of Arabidopsis. PLOS Genetics 2, e210
CrossRef | PubMed |

Schachtman DP, Schroeder JI (1994) Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants. Nature 370, 655–658.
CrossRef | CAS | PubMed |

Serrano R (1996) Salt tolerance in plants and microorganisms: toxicity targets and defense responses. International Review of Cytology 165, 1–52.
CrossRef | CAS | PubMed |

Shao Q, Zhao C, Han N, Wang BS (2008) Cloning and expression pattern of SsHKT1 encoding a putative cation transporter from halophyte Suaeda salsa. DNA Sequence 19, 106–114.
CrossRef | CAS | PubMed |

Su H, Balderas E, Rosario VE, Golldack D, Quigley F, Zhao CS, Pantoja O, Bohnert HJ (2003) Expression of the cation transporter McHKT1 in a halophyte. Plant Molecular Biology 52, 967–980.
CrossRef | CAS | PubMed |

Sunarpi , Horie T, Motoda J, Kubo M, Yang H, Yoda K, Horie R, Chan WY, Leung HY, Hattori K, Konomi M, Osumi M, Yamagami M, Schroeder JI, Uozumi N (2005) Enhanced salt tolerance mediated by AtHKT1 transporter induced Na+ unloading from xylem vessels to xylem parenchyma cells. The Plant Journal 44, 928–938.
CrossRef | CAS | PubMed |

Takahashi R, Liu S, Takano T (2007) Cloning and functional comparison of a high-affinity K+ transporter gene PhaHKT1 of salt-tolerant and salt-sensitive reed plants. Journal of Experimental Botany 58, 4387–4395.
CrossRef | CAS | PubMed |

Uozumi N, Kim EJ, Rubio F, Yamaguchi T, Muto S, Tsuboi A, Bakker EP, Nakamura T, Schroeder JI (2000) The Arabidopsis HKT1 gene homolog mediates inward Na+ currents in Xenopus laevis oocytes and Na+ uptake in Saccharomyces cerevisiae. Plant Physiology 122, 1249–1260.
CrossRef | CAS | PubMed |

Wang BS, Lüttge U, Ratajczak R (2001) Effect of salt treatment and osmotic stress on V-ATPase and V-PPase in leaves of the halophyte Suaeda salsa. Journal of Experimental Botany 52, 2355–2365.
CrossRef | CAS |

Subscriber Login

Legal & Privacy | Contact Us | Help


© CSIRO 1996-2015