Overexpression of human peroxisomal enoyl-CoA delta isomerase2 HsPECI2, an ortholog of bamboo expressed during gregarious flowering alters salinity stress responses and polar lipid content in tobacco
Vineeta Rai A B , Shayan Sarkar A , Suresh Satpati C and Nrisingha Dey A D
+ Author Affiliations
- Author Affiliations
A Division of Gene Function and Regulation, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751 023, Odisha, India.
B Present address: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, Maharashtra, India.
C Division of Translational Research and Technology Development, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751 023, Odisha, India.
D Corresponding author. Email: nrisinghad@gmail.com
Functional Plant Biology 43(3) 232-243 https://doi.org/10.1071/FP15292
Submitted: 18 September 2015 Accepted: 20 November 2015 Published: 1 February 2016
Abstract
Peroxisomal enoyl-CoA delta isomerase2 (PECI2) is one of the key enzymes that has critical role in lipid metabolism and plant development during salt stress. Seven out of ten tobacco plants overexpressing human PECI2 (HsPECI2) with PTS1-sequence showed hypersensitivity to salt. Under salt-stress, T2 transformed plants (HsPECI2) displayed reduced primary root, delayed shoot-growth, and visibly smaller rosette leaves turning pale yellow as compared to the pKYLX71 vector control plant. Also, we found altered reactive oxygen species (ROS) levels and reduced catalase activity in 100 mM sodium chloride (NaCl) treated HsPECI2 transformed plant compared with the pKYLX71 counterpart. ESI-MS/MS data showed that the polar lipids were differentially modulated upon salt treatment in HsPECI2 transformed and pKYLX71 plants as compared with the respective untreated counterpart. Notably, the levels of monogalactosyldiacylglycerol and phosphatidic acid varied significantly, whereas phosphatidylcholine, phosphatidylserine and digalactosyldiacylglycerol contents were moderately upregulated. In parallel, abscisic acid (ABA) responsiveness assay confirmed insensitivity of HsPECI2 transformed plant towards ABA. Overall our data proclaim that HsPECI2 play multifunctional role in normal development and response to salinity stress apart from its primary role in β-oxidation.
Additional keywords: exogenous ABA, lipid profiling, peroxisomal enoyl-CoA delta isomerase2, salinity stress, tobacco transgenic plants.
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