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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Expression and functional analysis of PhEOL1 and PhEOL2 during flower senescence in petunia

Juanxu Liu A B , Ji Zhao A , Zhina Xiao A , Xinlei Chang A , Guoju Chen B and Yixun Yu A B C
+ Author Affiliations
- Author Affiliations

A Guangdong Key Laboratory for Innovative Development and Utilisation of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.

B College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

C Corresponding author. Email: yuyixun@scau.edu.cn

Functional Plant Biology 43(5) 413-422 https://doi.org/10.1071/FP15311
Submitted: 30 September 2015  Accepted: 8 January 2016   Published: 29 February 2016

Abstract

The ethylene biosynthesis pathway controls flower senescence. Previous studies have shown that Arabidopsis ETHYLENE-OVERPRODUCER1 (ETO1) interacts specifically with and negatively regulates type 2 1-aminocyclopropane-1-carboxylate synthases (ACSs), the rate-limiting enzymes of ethylene biosynthesis. The ethylene biosynthesis pathway controls flower senescence in petunias (Petunia hybrida Juss.). However, the role of ETO1-like genes (EOLs) during flower senescence has not been investigated. Here, two full-length petunia EOL cDNAs, PhEOL1 and PhEOL2, were isolated. RT–PCR assays indicated that the expression of PhEOL1 and PhEOL2 increased after exogenous ethylene treatment. The VIGS-mediated silencing of PhEOL1 accelerated flower senescence and produced more ethylene than the control condition, whereas the silencing of PhEOL2 did not. Notably, the effects caused by PhEOL1 suppression were not enhanced by PhEOL2 suppression in corollas. In addition, the expression of two petunia type 2 PhACS genes increased during flower senescence and after ethylene treatment. A yeast two-hybrid assay showed that PhEOL1 interacts with both PhACS2 and PhACS3. It is possible that PhEOL1 is involved in flower senescence by interacting with type 2 PhACSs in petunias.

Additional keywords: ACC synthase, ethylene, ETO1-like, Petunia, VIGS.


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