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Plant function and evolutionary biology
RESEARCH ARTICLE

Phosphite-induced reactive oxygen species production and ethylene and ABA biosynthesis, mediate the control of Phytophthora capsici in pepper (Capsicum annuum)

Peiqing Liu A C , Benjin Li A C , Ming Lin B , Guoliang Chen B , Xueling Ding A C , Qiyong Weng A C D and Qinghe Chen A B C D
+ Author Affiliations
- Author Affiliations

A Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, 350 003, China.

B Fujian-Taiwan Joint Innovative Centre for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou 350 002, China.

C Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, 350 003, China.

D Corresponding authors. Emails: wengqy@faas.cn; chenqh@faas.cn

Functional Plant Biology 43(6) 563-574 https://doi.org/10.1071/FP16006
Submitted: 7 January 2016  Accepted: 15 April 2016   Published: 12 May 2016

Abstract

Phytophthora capsici is an oomycete pathogen with a broad host range that inflicts significant damage in vegetables. Phosphite (Phi) is used to control oomycete diseases, but the molecular mechanisms underlying Phi-induced resistance to P. capsici are unknown. Thus, Phi-inhibited mycelial growth on strain LT1534 and primed host defence were analysed. We demonstrated that Phi (>5 µg mL–1) had a direct antibiotic effect on mycelial growth and zoospore production, and that mortality and DNA content were significantly reduced by pre-treatment with Phi. In addition, elevated hydrogen peroxide (H2O2) promoted callose deposition and increased the levels of soluble proteins and Capsicum annuum L. pathogenesis-related 1 (CaPR1) expression. Furthermore, Phi (1 g L–1) significantly increased the transcription of the antioxidant enzyme genes, and the genes involved in ethylene (ET) and abscisic acid (ABA) biosynthesis, as well as mitogen-activated protein kinase (MAPK) cascades. However, pre-treatment with reactive oxygen species (ROS), ABA and ET biosynthesis inhibitors decreased Phi-induced resistance and reduced the expression of ABA-responsive 1 (CaABR1) and lipoxygenase 1 (CaLOX1). In addition, the decreased ROS and ABA inhibited Phi-induced expression of CaMPK171. We propose that Phi-induced ROS production, ET and ABA biosynthesis mediate the control of P. capsici, and that ABA functions through CaMPK171-mediated MAPK signalling.

Additional keywords: ET, ABA, phosphite, ROS.


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