Regulation of the expressions of HCO₃^- uptake and intracellular carbonic anhydrase in response to CO₂ concentration in the marine diatom Phaeodactylum sp.

Yusuke Matsuda, Keiichi Satoh, Hisashi Harada, Dan Satoh, Yasutaka Hiraoka and Takumi Hara

Abstract

The marine diatom, Phaeodactylum tricornutum Bohlin, is probably one of the most extensively studied marine alga with respect to carbon acquisition and assimilation mechanisms. However, quantitative analyses of HCO₃^-utilization and the detailed process of acclimation of cells from high CO₂ to limited CO₂ are yet to be done extensively. Suitable molecular markers for this acclimation process are not established, either. Recently, it became clear that the rate of CO₂ formation in artificial seawater is about eight times slower than that in freshwater, and thatP. tricornutum cells utilize HCO₃^- quite efficiently. Despite their great capacity to take up HCO₃^-, the signal controlling photosynthetic affinity for dissolved inorganic carbon has been shown to be CO₂ in the medium. Furthermore, light seems to be required for this process.

Internal carbonic anhydrase (CA) activity has been shown to be crucial for high-affinity photosynthesis in a number of algae, including marine diatoms. Internal β-type CA, which has been isolated in one strain of P. tricornutum, was clearly shown to be a low-CO₂ inducible enzyme. This review paper additionally includes data showing that this CA occurs generally in P. tricornutum species.

Keywords: carbonic anhydrase, CO2 sensing, HCO3- uptake, marine diatom,Phaeodactylum tricornutum, photosynthesis.

Functional Plant Biology 29(3) 279 - 287 (2002) doi:10.1071/PP01186