Phylogeny of the Australian freshwater crayfish Cherax destructor-complex (Decapoda : Parastacidae) inferred from four mitochondrial gene regions

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doi:10.1071/IS04021

Phylogeny of the Australian freshwater crayfish Cherax destructor-complex (Decapoda : Parastacidae) inferred from four mitochondrial gene regions

Thuy T. T. Nguyen ^A ^B ^C, Christopher M. Austin ^A

^A School of Ecology and Environment, Deakin University, Warrnambool, Victoria 3280, Australia.
^B Present address: Network of Aquaculture Centres in Asia-Pacific, PO Box 1040, Kasetsart Post Office, Bangkok 10903, Thailand.
^C Corresponding author. Email: thuy.nguyen@enaca.org





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Invertebrate Systematics 19(3) 209–216 http://dx.doi.org/10.1071/IS04021
Submitted: 16 August 2004 Accepted: 11 April 2005 Published online: 15 July 2005

Abstract

The phylogenetic relationships among 32 individuals of Australian freshwater crayfish belonging to the Cherax destructor-complex were investigated using a dataset comprising sequences from four mitochondrial gene regions: the large subunit rRNA (16S rRNA), cytochrome oxidase I (COI), adenosine triphosphatase 6 (ATPase 6), and cytochrome oxidase III (COIII). A total of 1602 bp was obtained, and a combined analysis of the data produced a tree with strong support (bootstrap values 94–100%) for three divergent lineages, verifying the phylogenetic hypotheses of relationships within the C. destructor species-complex suggested in previous studies. Overall, sequences from the 16S rRNA gene showed the least variation compared to those generated from protein coding genes, which presented considerably greater levels of divergence. The level of divergence within C. destructor was found to be greater than that observed in other species of freshwater crayfish, but interspecific variation among species examined in the present study was similar to that reported previously.