Identification of marine fish eggs in Taiwan using light microscopy, scanning electric microscopy and mtDNA sequencing
Kwang-Tsao Shao, Kang-Ching Chen and Jui-Hsien Wu
Marine and Freshwater Research 53(2) 355 - 365
Abstract
A previously published study used light microscopy, SEM and mtDNA sequencing
to identify eggs of 104 taxa to species, 24 to genus and 67 to family levels;
28 of the species were identified by hatching methods and 23 species of eggs
were obtained from aquaculture farms (in which the species were known).
Light-microscopic characters of 10 species that have not previously been
reported are described in the present paper. It is concluded that
(1) more diagnostic characters can be found by SEM than
by light microscopy, (2) different species may share
identical egg morphology even under SEM, so identification using morphological
characters is better applied to the categories of family or genus only, not to
species, and (3) in addition to the hatching method,
molecular sequencing is an alternative way to assign an egg to a species as
well as to verify the correctness of previous morphological identification.
This necessitates use of the same egg for both morphological observations and
DNA sequencing. Its feasibility and experimental process are demonstrated in
the present paper using lizardfish (Synodontidae) as an example.
Full text doi:10.1071/MF01141
© CSIRO 2002





Early Alert
Connect with us





