Differences in stream nutrient concentrations typically reflect upstream differences in land use. In particular, nitrate concentrations are greatly increased by losses from nitrogen (N) fertiliser applied to areas of intensive cropping. In the present study, a relationship between the area of such land use and the nitrate concentrations in the receiving streams was predicted. This relationship was tested using several data sets from the Tully basin, in the wet-tropics bioregion of north Queensland, Australia. The proportions of fertiliser-additive land use (FALU), mostly sugarcane and bananas, were correlated with the concentrations of nutrients in streams that drain these land uses. The data compared included two long-term sampling studies in the Tully River catchment and more recent, broader catchment sampling and plot-scale studies in this region. A strong relationship was shown for nitrate, but weaker relationships were observed for other N-nutrient and P-nutrient forms. Comparisons were made with contemporary and historical land-use changes in the Tully basin. The strong relationship of FALU with nitrate provides evidence that the nitrate exports from this catchment are largely derived from fertiliser use. This relationship can be used to derive nitrate run-off coefficients for fertilised land use in catchment models or to monitor changes following management to reduce fertiliser usage.