To maximise bat retention in urban environments, efficient bat monitoring is needed, but the factors that influence survey results for urban bats are unclear. We used echolocation call detectors (n = 378 detector-nights from November 2005 to October 2006) to assess bat activity among different sites in the Adelaide City parklands, temporal variations in activity (hourly, nightly, monthly), and responses to weather and light (artificial and moon). Bat species did not occur evenly in urban conditions; dark parks were more important for bat diversity and activity (six species groups in the darkest park) than were artificially lit parkland areas (three species groups in the flood-lit park). Gould’s wattled bat (Chalinolobus gouldii) and Mormopterus species 4 (94% of calls) were advantaged in urban parklands, being the only species recorded when lights were on at sports parks, whereas five species groups occurred when the lights were off. Minimal bat activity was recorded in the first 2 h after civil twilight, suggesting that bats may roost outside the city and commute nightly into parklands. Bat activity increased with temperature, with a burst in activity occurring after 7°C. Rainfall (>1 mm/24 h) and moon illumination at midnight did not influence activity. Urban environments should provide diversity to attract a diverse assemblage of bat species. Activity fluctuated among sites, nights, and across the year, indicating that large sample sizes over long periods of time are required to monitor and survey bats reliably with detectors.