320 pages, 230 x 155 mm
RNA interference (RNAi) has been one of the most exciting and significant new methodologies to appear in the past decade, and it now finds widespread application. This methods manual provides the theoretical background to experimental approaches, followed by bullet-point protocols, which are generic and can be adapted for particular systems. It provides an introduction to the phenomenon to RNA Interference and specific protocols for RNAi, in organisms from plants C.elegans to Drosophila and mammals. Also included are chapters covering small hairpin RNAs and viral-induced gene silencing.
1. General Introduction
2. RNAi in Plants
4. Synthetic miRNAs in Plants
5. RNAi in Caenorhabditus elegens
6. RNAi in Drosophila
7. RNAi in Zebra Fish
8. RNAi in Avian Embryos (in ovo)
9. RNAi in Mammalian Cells
Students and researchers working in functional genomics within all fields of the life sciences including upper level undergraduates, postgraduates and researchers.