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The South Pacific Journal of Natural and Applied Sciences The South Pacific Journal of Natural and Applied Sciences Society
Research and review papers in the area of science, engineering and mathematics
RESEARCH ARTICLE

In vitro micro propagation of Nicotiana benthamiana via axillary shoots

Pradeep C. Deo, Benjamin Dugdale, Robert M. Harding, Maiko Kato and James Dale

The South Pacific Journal of Natural and Applied Sciences 32(2) 55 - 60
Published: 26 March 2015

Abstract

Axillary shoots of Nicotiana benthamiana were regenerated from nodal explants in two weeks using MS media supplemented with the cytokinin, kinetin (0.5 mg/L), and the auxin, indole-3-butyric acid (IBA) (0.1 mg/L). Ninety two percent of shoots were 2.1-20 mm tall, a size ideal for root induction. After transfer to hormone-free MS they readily produced roots within seven days, with phenotypically normal, fully developed plants being obtained within four weeks. Leaf chlorosis due to iron deficiency was observed in plants over time, however, this was overcome by doubling the concentration of inorganic iron. This rapid micro-propagation system is particularly useful for the in vitro mass production of N. benthamiana plants for various biotechnological applications.

https://doi.org/10.1071/SP14009

© The University of the South Pacific 2015

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