Analysis of diversity within Fusarium oxysporum populations using molecular and vegetative compatibility grouping
J. L. Smith-White, L. V. Gunn and B. A. Summerell
Australasian Plant Pathology 30(2) 153 - 157
Abstract
DNA fingerprints, based on Enterobacterial Repetitive Intergenic Consensus
(ERIC) sites, were investigated as an alternative technique to Vegetative
Compatibility Grouping (VCG) in studying populations of
Fusarium oxysporum Schlecht. emend. Snyd. & Hans.
Isolates of F. oxysporum were recovered from orchids
displaying visible signs of root rot. Fifty-two isolates were collected and
analysed using both the ERIC DNA fingerprinting technique and Vegetative
Compatibility Grouping. Isolates were divided into 17 ERIC groups and 37 VCG
groups. Based on its lower level of sensitivity and its rapid and reproducible
result, the ERIC DNA fingerprinting was found to be a more appropriate
technique for studying diverse populations of
F. oxysporum.
Keywords: DNA fingerprinting, ERIC, Vegetative Compatibility Grouping
Full text doi:10.1071/AP01014
© CSIRO 2001





Australasian Plant Disease Notes
