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 Australasian Plant Disease Notes
Disease notes, new records and quarantine interception reports are published in Australasian Plant Disease Notes.

 

Article << Previous     |     Next >>   Contents Vol 30(2)

Analysis of diversity within Fusarium oxysporum populations using molecular and vegetative compatibility grouping

J. L. Smith-White, L. V. Gunn and B. A. Summerell

Australasian Plant Pathology 30(2) 153 - 157

Abstract

DNA fingerprints, based on Enterobacterial Repetitive Intergenic Consensus (ERIC) sites, were investigated as an alternative technique to Vegetative Compatibility Grouping (VCG) in studying populations of Fusarium oxysporum Schlecht. emend. Snyd. & Hans. Isolates of F. oxysporum were recovered from orchids displaying visible signs of root rot. Fifty-two isolates were collected and analysed using both the ERIC DNA fingerprinting technique and Vegetative Compatibility Grouping. Isolates were divided into 17 ERIC groups and 37 VCG groups. Based on its lower level of sensitivity and its rapid and reproducible result, the ERIC DNA fingerprinting was found to be a more appropriate technique for studying diverse populations of F. oxysporum.

Keywords: DNA fingerprinting, ERIC, Vegetative Compatibility Grouping



Full text doi:10.1071/AP01014

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