Host range, symptom expression and RNA 3 sequence analyses of six Australian strains of Cucumber mosaic virus

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Contents Vol 33(4)

doi:10.1071/AP04054

Host range, symptom expression and RNA 3 sequence analyses of six Australian strains of Cucumber mosaic virus

Emy Sulistyowati ^A ^B ^C ^G, Neena Mitter ^A ^G, Shanna Bastiaan-Net ^A ^D, Marilyn J. Roossinck ^E, Ralf G. Dietzgen ^A ^B ^F

^A Department of Primary Industries and Fisheries, Queensland Agricultural Biotechnology Centre, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Qld 4067, Australia.
^B The University of Queensland, Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, St Lucia, Qld 4072, Australia.
^C Present address: Research Institute for Tobacco and Fibre Crops, PO Box 199, Malang, Indonesia 65152.
^D Present address: Department of Molecular Plant Physiology, Utrecht University, 3584 CH Utrecht, The Netherlands.
^E The S.R. Noble Foundation, PO Box 2180, Ardmore OK 73402, USA.
^F Corresponding author. Email: ralf.dietzgen@dpi.qld.gov.au
^G The first two authors contributed equally to this work.





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Australasian Plant Pathology 33(4) 505–512 http://dx.doi.org/10.1071/AP04054
Submitted: 11 February 2004 Accepted: 8 April 2004 Published online: 9 December 2004

Abstract

We have characterised six Australian Cucumber mosaic virus (CMV) strains belonging to different subgroups, determined by the sequence of their complete RNA 3 and by their host range and the symptoms they cause on species in the Solanaceae, Cucurbitaceae and on sweet corn. These data allowed classification of strains into the known three CMV subgroups and identification of plant species able to differentiate the Australian strains by symptoms and host range. Western Australian strains 237 and Twa and Queensland strains 207 and 242 are closely related members of CMV subgroup IA, which cause similar severe symptoms on Nicotiana species. Strains 207 and 237 (subgroup IA) were the only strains tested which systemically infected sweet corn. Strain 243 caused the most severe symptoms of all strains on Nicotiana species, tomato and capsicum and appears to be the first confirmed subgroup IB strain reported in Australia. Based on pair-wise distance analysis and phylogeny of RNA 3, as well as mild disease symptoms on Nicotiana species, CMV 241 was assigned to subgroup II, as the previously described Q-CMV and LY-CMV.

Keywords: host differentials, phylogeny, reverse transcription–polymerase chain reaction, serogroup.