RAPD-PCR used to confirm that four pectic isozyme (zymogram) groups within the Australian Rhizoctonia solani AG 8 population are true intraspecific groups

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Contents Vol 34(2)

doi:10.1071/AP05004

RAPD-PCR used to confirm that four pectic isozyme (zymogram) groups within the Australian Rhizoctonia solani AG 8 population are true intraspecific groups

G. C. MacNish ^A, P. A. O’Brien ^A ^B

^A School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia.
^B Corresponding author. Email: obrien@murdoch.edu.au





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Australasian Plant Pathology 34(2) 245–250 http://dx.doi.org/10.1071/AP05004
Submitted: 17 May 2004 Accepted: 5 October 2004 Published online: 22 June 2005

Abstract

RAPD-PCRs were used to study 79 isolates of Rhizoctonia solani AG 8 representing four pectic isozyme groups [zymogram (ZG)] (ZG1-1, 1-2, 1-4 and 1-5) from various locations in southern Australia. RAPD-PCR was performed on all isolates using six primers and the relatedness of the isolates determined by generating a neighbour joining tree. All isolates were also grouped according to vegetatively compatible populations (VCPs). Within each ZG there were four to nine VCPs. These studies show that the AG 8 population contains four distinct groups that matched the four ZGs and support the concept that ZGs are distinct intraspecific groups.