Leaf-clearing and staining techniques for the observation of conidiophores in the Phyllactinioideae (Erysiphaceae)

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Contents Vol 34(3)

doi:10.1071/AP05027

Leaf-clearing and staining techniques for the observation of conidiophores in the Phyllactinioideae (Erysiphaceae)

J. R. Liberato ^A ^C, R. W. Barreto ^B, R. G. Shivas ^A

^A Department of Primary Industries and Fisheries, Plant Pathology Herbarium, 80 Meiers Road, Indooroopilly, Qld 4068, Australia.
^B Universidade Federal de Viçosa, Departamento de Fitopatologia, 36571-000, Viçosa, MG, Brazil.
^C Corresponding author. Email: jose.liberato@dpi.qld.gov.au





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Australasian Plant Pathology 34(3) 401–404 http://dx.doi.org/10.1071/AP05027
Submitted: 18 October 2004 Accepted: 1 February 2005 Published online: 16 September 2005

Abstract

Some whole leaf-clearing and staining techniques are described for the microscopic observation of the origin of powdery mildew conidiophores, whether from external mycelium or internal mycelium, emerging through stomata. These techniques enable separation of the two genera, Oidiopsis and Streptopodium, in the Erysiphaceae.

Keywords: Ovulariopsis.