First record of a phytoplasma-associated disease of chickpea (Cicer arietinum) in Australia

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doi:10.1071/AP05047

First record of a phytoplasma-associated disease of chickpea (Cicer arietinum) in Australia

M. Saqib ^A ^B, K. L. Bayliss ^B, B. Dell ^B, G. E. St J. Hardy ^B, M. G. K. Jones ^A ^B ^C

^A WA State Agricultural Biotechnology Centre, Murdoch University, Perth, WA 6150, Australia.
^B School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia.
^C Corresponding author. Email: m.jones@murdoch.edu.au





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Australasian Plant Pathology 34(3) 425–426 http://dx.doi.org/10.1071/AP05047
Accepted: 21 April 2005 Published online: 16 September 2005

Abstract

Chickpeas growing in the agricultural region of Kununurra, Western Australia, were found with symptoms of leaf stunting, little leaf and proliferating branches. Deoxyribonucleic acid was extracted from symptomatic and asymptomatic plants and tested for phytoplasma by PCR. The PCR product from symptomatic leaves was sequenced and confirmed the presence of a phytoplasma with high similarity to the 16SrII group ‘Candidatus Phytoplasma aurantifolia’. This is the first molecular evidence for a phytoplasma-associated disease in chickpea.

Keywords: kabuli, Candidatus Phytoplasma aurantifolia.