Characterisation and pathogenicity of Cylindrocladiella spp. associated with root and cutting rot symptoms of grapevines in nurseries

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Contents Vol 34(4)

doi:10.1071/AP05058

Characterisation and pathogenicity of Cylindrocladiella spp. associated with root and cutting rot symptoms of grapevines in nurseries

G. J. van Coller ^A ^B, S. Denman ^A ^C, J. Z. Groenewald ^A ^D, S. C. Lamprecht ^A ^E, P. W. Crous ^A ^D ^F

^A Department of Plant Pathology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa.
^B Current address: Western Cape Department of Agriculture, Private Bag X1, Elsenburg 7607, South Africa.
^C Forest Research Station, Alice Holt Lodge, Farnham, Surrey, GU10 4LH, UK.
^D Centraalbureau voor Scimmelcultures, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.
^E ARC-PPRI, Vredenburg, Stellenbosch 7602, South Africa.
^F Corresponding author. Email: crous@cbs.knaw.nl





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Australasian Plant Pathology 34(4) 489–498 http://dx.doi.org/10.1071/AP05058
Submitted: 12 October 2004 Accepted: 1 May 2005 Published online: 2 December 2005

Abstract

Species of Cylindrocladiella occur on a variety of hosts, where they are known to act as pathogens or saprobes. Eight species from this genus are currently recognised, of which five have been reported previously in South Africa: C. camelliae, C. elegans, C. lageniformis, C. parva and C. peruviana. Isolates of Cylindrocladiella were obtained from a newly established 99-Richter grapevine mother vine block exhibiting decline symptoms. The DNA phylogeny of these and additional isolates, also obtained from declining grapevines, was determined by sequencing the ITS (ITS1, ITS2 and 5.8S) as well as β-tubulin and histone H3 gene regions. This identified four species of Cylindrocladiella on grapevines in South Africa: C. lageniformis, C. parva, C. peruviana and a new species described here as C. viticola, which forms part of the C. infestans species complex. Pathogenicity trials, using stem inoculations on green and 1-year-old 99-Richter cuttings to determine the pathogenicity of selected isolates of these species, gave inconclusive results.

Keywords: Cylindrocladiella viticola, 99-Richter, 101–14 Mgt.