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Detection of Odontoglossum ringspot virus in orchids from Sikkim, India
A. R.
Sherpa A,
T. K.
Bag B,
V.
Hallan A,
A. A.
Zaidi A C
A
Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology (CSIR), Post Box No. 6, Palampur 176061, Himachal Pradesh, India.
B
National Research Centre for Orchids (ICAR), Pakyong, East Sikkim, 737 106, India.
C
Corresponding author. Email: zaidi_aijaz@yahoo.com
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Australasian Plant Pathology 35(1) 69–71 http://dx.doi.org/10.1071/AP05094
Submitted: 22 June 2005
Accepted: 11 October 2005
Published online: 23 January 2006
Abstract
Odontoglossum ringspot virus (ORSV) is the most prevalent and economically important virus of orchids in the world. However, little is known of the incidence of this virus in orchid collections in India. We describe development of a slot-blot hybridisation assay for ORSV. With this test, 42 of 100 orchid samples from Sikkim in north-eastern India tested positive for the virus. This assay is useful for the screening and certification of virus-free orchid material (for distribution to commercial growers) due to its high sensitivity, reliability and efficacy.
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Australasian Plant Disease Notes
