Survey for Grapevine fanleaf virus in vineyards of north-west Iran and genetic diversity of isolates

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Contents Vol 36(1)

doi:10.1071/AP06080

Survey for Grapevine fanleaf virus in vineyards of north-west Iran and genetic diversity of isolates

N. S. Bashir ^A ^B, M. Hajizadeh ^A

^A Plant Protection Department, University of Tabriz, Tabriz 51664, Iran.
^B Corresponding author. Email: sokhandan@tabrizu.ac.ir





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Australasian Plant Pathology 36(1) 46–52 http://dx.doi.org/10.1071/AP06080
Submitted: 10 May 2006 Accepted: 29 September 2006 Published online: 19 January 2007

Abstract

Grapevine fanleaf virus (genus Nepovirus) was detected by double antibody sandwich enzyme-linked immunosorbant assay (DAS-ELISA) in 31 of 134 grapevine samples from the north-west of Iran. Reverse transcriptase polymerase chain reaction with two primer sets (S2515/A3300 and CP433V/912C) targeting the Grapevine fanleaf virus coat protein gene resulted in amplification of the expected DNA fragments from some but not all DAS-ELISA positive samples. At the nucleotide level, the Iranian isolates were 84–95% identical to each other and 84–91% identical to other isolates from around the world. This is the first comprehensive analysis of the distribution and genetic diversity of Grapevine fanleaf virus in Iran.

Keywords: certification scheme, phylogenetic analysis.