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A PCR-based method to detect Sclerotium hydrophilum in infected rice leaf sheaths
C. J.
Hu A B,
Y. R.
Li B,
Y. W.
Wei B,
S. L.
Huang A C D E
A
Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China.
B
Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning 530007, Guangxi, China.
C
Nanyang Normal University, Nanyang 473061, China.
D
Guangxi University, Nanning 530005, Guangxi, China.
E
Corresponding author. Email: silianghuang@126.com
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Australasian Plant Pathology 37(1) 40–42 http://dx.doi.org/10.1071/AP07076
Submitted: 5 June 2007
Accepted: 18 September 2007
Published online: 3 January 2008
Abstract
An oligonucleotide primer pair set based on internal transcribed spacer regions of rDNA for PCR, which gives the amplicon for only the DNA from Sclerotium hydrophilum was designed. Tests on the specificity of the primers concerned showed that a single product of 540 bp was amplified from the DNA of S. hydrophilum but not from the DNA of any other species responsible for sheath disease in rice, suggesting that the primer pair could be used to develop a PCR-based diagnostic method to provide a rapid and accurate molecular detection of this pathogen in rice leaf sheath.
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Australasian Plant Disease Notes
