Identification of specific molecular markers linked to the rust resistance gene M4 in flax

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doi:10.1071/AP08032

Identification of specific molecular markers linked to the rust resistance gene M4 in flax

T. Y. Bo ^A ^B ^D, J. J. Ma ^A ^B, J. X. Chen ^A ^B, T. Y. Miao ^A ^B, W. X. Zhai ^C

^A Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, 201106 Shanghai, China.
^B Shanghai Key Lab of Protected Horticultural Technology, 201106 Shanghai, China.
^C Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101 Beijing, China.
^D Corresponding author. Email: tybo@saas.sh.cn





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Australasian Plant Pathology 37(4) 417–420 http://dx.doi.org/10.1071/AP08032
Submitted: 15 February 2008 Accepted: 20 March 2008 Published online: 11 June 2008

Abstract

To identify molecular markers linked to the flax rust-resistance gene M4, RAPD analysis of NM4 (a near-isogenic line containing the M4 gene) and the recurrent parent Bison was performed using 540 decamer primers. The primer OPA18 amplified a specific fragment, OPA18₄₃₂, in the NM4 line. The OPA18₄₃₂ marker was found to be closely linked to the M4 gene, with a genetic distance of 2.1 cM, through the analysis of the F₂ segregating population derived from a cross of Bison × NM4. Based on the sequence of OPA18₄₃₂, the specific PCR primers were designed and a SCAR marker for the M4 gene was developed. The amplification of different resistant germplasm proved that the marker is specific for the M4 gene. This marker has been used successfully in marker-assisted selection in the flax breeding program.