Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs
A. McLauchlan, F. C. Ogbonnaya, B. Hollingsworth, M. Carter, K. R. Gale, R. J. Henry, T. A. Holton, M. K. Morell, L. R. Rampling, P. J. Sharp, M. R. Shariflou, M. G. K. Jones and R. Appels
Australian Journal of Agricultural Research 52(12) 1409 - 1416
Abstract
The absence of expression of the granule-bound starch synthase I (GBSSI)
allele from chromosome 4A of wheat is associated with improved starch quality
for making Udon noodles. Several PCR-based methods for the analysis of GBSS
alleles have been developed for application in wheat. A widely applied
approach has involved a simple PCR followed by electrophoretic separation of
DNA products on agarose gels. The PCR amplifies one band from each of the loci
on chromosomes 4A (Wx-B1), 7A
(Wx-A1), and 7D (Wx-D1), and the
band from the Wx-B1 locus is diagnostic for the
occurrence of the null Wx-B1 allele that is associated
with improved starch quality. The reliable detection of the null
Wx-B1 allele has been important in identifying wheat
breeding lines. Allele-specific PCR has also been used to successfully detect
the occurrence of the null Wx-B1 allele. In the present
paper the various protocols were evaluated by testing a segregating double
haploid population from a cross between Cranbrook and Halberd and the tests
gave good agreement in different laboratories. The application of the DNAbased
tests applied in wheat breeding programs provides one of the first examples of
a molecular marker selection for a grain quality trait being successfully
applied in an Australian wheat breeding program.
Keywords: grain quality, marker assisted selection, waxy.
Full text doi:10.1071/AR01036
© CSIRO 2001





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