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Article << Previous     |     Next >>   Contents Vol 55(5)

Fruit production in kiwifruit (Actinidia deliciosa) using preserved pollen

I. Abreu A B C, M. Oliveira A

A Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal.
B Departamento de Botânica – FCUP, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal.
C Corresponding author: email: inoronha@ibmc.up.pt
 
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Abstract

The influence of temperature and storage period on pollen quality of Actinidia deliciosa (A. Chev.) C. F. Liang & A. R. Ferguson was studied. Pollen collected from male flowers was stored under different conditions (20°C, 65% RH; –20°C, 51% RH; –80°C, 55% RH and –196°C by direct immersion in liquid nitrogen). During the preservation period, viability and in vitro germination percentages were evaluated at regular periods.

The results show that –20°C was the best temperature at which to preserve pollen of A. deliciosa because it retains high viability and germination. At 20°C, germination was totally lost within 8 weeks, and at –80°C or –196°C germination drastically dropped over the same period of preservation.

In the 2002 blossoming season, the pollen preserved at –20°C was used for hand pollination in order to estimate its seed set capacity. Thirty days after fruit-set, all fruits were long and well shaped, indicating a successful pollination. Our results indicate a simple and reliable method to preserve pollen of A. deliciosa that can be easily used by farmers.

Keywords: fruit-set, hand pollination, kiwifruit, pollen germination, pollen viability, wind pollination.


   
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