Ethanol soluble proteins were extracted from 5 different triticale varieties grown at 2 different sites in South Australia, and from single samples of wheat and rye. Separation of these proteins using capillary electrophoresis (CE) showed different patterns for different varieties but similar patterns for the same varieties grown at the 2 sites. The main differences between electropherograms for wheat and triticale were attributed to the presence of rye secalins, higher levels of α-gliadins, and lower levels of β-gliadins in those from the latter. These results indicate the potential of CE of ethanol-soluble proteins for identification of triticale varieties.