doi:10.1071/AR04140

The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato

S. Garland ^A ^B, M. Sharman ^A, D. Persley ^A, D. McGrath ^A

^A Department of Primary Industries and Fisheries, GPO Box 46, Brisbane, Qld 4001, Australia.
^B Corresponding author. Email: Stephen.Garland@dpi.qld.gov.au





	PDF (187 KB) $25



	Export Citation

Print

Australian Journal of Agricultural Research 56(3) 285–289 http://dx.doi.org/10.1071/AR04140
Submitted: 21 June 2004 Accepted: 27 January 2005 Published online: 23 March 2005

Abstract

Sw-5 is an important disease resistance gene of tomato, providing broad resistance to Tomato spotted wilt virus (TSWV). A cleaved amplified polymorphic sequence (CAPS) marker, closely linked to the gene, has been reported. Although the Sw-5 locus has been characterised, a gene-specific marker has not been developed. This paper presents a PCR-based marker-system that consists of the co-amplification of a dominant marker representing the Sw-5 gene sequence, and the modified CAPS marker as a positive control and indicator of genotype.

Keywords: rapid DNA extraction.

Article

<< Previous

Next >>

Contents Vol 56(3)