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RESEARCH ARTICLE

Agronomic advantages conferred by endophyte infection of perennial ryegrass (Lolium perenne L.) and tall fescue (Festuca arundinacea Schreb.) in Australia

D. E. Hume A C and J. C. Sewell B
+ Author Affiliations
- Author Affiliations

A AgResearch, Palmerston North, 4442, New Zealand.

B PGG Wrightson Seeds, Ballarat, Vic. 3352, Australia.

C Corresponding author. Email: david.hume@agresearch.co.nz

Crop and Pasture Science 65(8) 747-757 https://doi.org/10.1071/CP13383
Submitted: 11 November 2013  Accepted: 15 January 2014   Published: 18 June 2014

Abstract

Perennial ryegrass and tall fescue are key grasses of sown pastures in the high-rainfall zone of south-eastern Australia. Ryegrass in naturalised pastures, and in sown seed, is widely infected with Neotyphodium fungal endophytes, with toxic endophyte strains occasionally causing toxicosis in livestock. Endophyte infection is also beneficial in sown grasslands, assisting ryegrass hosts to overcome biotic stresses, and tall fescue hosts to overcome biotic and abiotic stresses. We review the literature for Australia and present new data, to examine the agronomic effects of endophyte. Frequency of endophyte infection in old, perennial ryegrass pastures and ecotype-based cultivars is high and, in all pastures, increases with time, providing evidence for endophyte-infected plants having an agronomic advantage over endophyte-free plants. Within a cultivar, agronomic field experiments have compared endophyte-infected with endophyte-free swards. Endophyte significantly improved ryegrass establishment in seven of 19 measurements taken from 12 trials. In mature ryegrass pastures, over half of the experiments found advantages to endophyte infection. Tall fescues infected with a selected endophyte (‘AR542’) had improved agronomic performance relative to endophyte-free in a majority of experiments, and on occasions, the endophyte was essential for tall fescue persistence. Cultivar × endophyte interactions occurred but were inconsistent. In high-stress environments, endophyte was more important for agronomic performance than difference between cultivars. The relative importance of cultivar and endophyte is discussed, with elite cultivars that are adapted to the region and are infected with elite endophytes being the best avenue to capture the benefits and minimise detrimental endophyte effects on livestock. The major drivers are likely to be insect pests and drought, but evidence is limited.

Additional keywords: dry matter yield, Festuca arundinacea, grass population, Lolium perenne, Neotyphodium coenophialum, Neotyphodium lolii.


References

Boschma SP, Lodge GM, Harden S (2009) Establishment and persistence of perennial grass and herb cultivars and lines in a recharge area, North-West Slopes, New South Wales. Crop & Pasture Science 60, 753–767.
Establishment and persistence of perennial grass and herb cultivars and lines in a recharge area, North-West Slopes, New South Wales.Crossref | GoogleScholarGoogle Scholar |

Bourke CA, Hunt E, Watson R (2009) Fescue-associated oedema of horses grazing on endophyte-inoculated tall fescue grass (Festuca arundinacea) pastures. Australian Veterinary Journal 87, 492–498.
Fescue-associated oedema of horses grazing on endophyte-inoculated tall fescue grass (Festuca arundinacea) pastures.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1Mjns1Klug%3D%3D&md5=6178d66c891840ca9506b22cb077485dCAS | 19930166PubMed |

Bouton JH, Latch GCM, Hill NS, Hoveland CS, McCann MA, Watson RH, Parish JA, Hawkins LL, Thompson FN (2002) Reinfection of tall fescue cultivars with non-ergot alkaloid producing endophytes. Agronomy Journal 94, 567–574.
Reinfection of tall fescue cultivars with non-ergot alkaloid producing endophytes.Crossref | GoogleScholarGoogle Scholar |

Clark SG, Reed KFM (1989) ‘Pasture grasses ’89: results of grass cultivar trials established 1986–88.’ (Department of Agriculture and Rural Affairs: Hamilton, Vic.)

Clark SG, Reed KFM, Cunningham PJ, Kelly KB, Savage GJ, Hirth JR, Griffiths JB (1990) ‘Pasture grasses ’90: results of grass cultivar trials established 1987–89.’ (Department of Agriculture and Rural Affairs: Hamilton, Vic.)

Cosgrove GP, Anderson CB, Phillot M, Nyfeler D, Hume DE, Parsons AJ, Lane GA (2002) The effects of endophyte alkaloids on diet selection by sheep. Proceedings of the New Zealand Society of Animal Production 62, 167–170.

Cunningham PJ (1988) Productivity and persistence of perennial ryegrass at low rainfall sites in southwest Victoria. In ‘Research review 1987/88. Pastoral Research Institute, Hamilton’ (Department of Agriculture and Rural Affairs: Hamilton, Vic.)

Cunningham PJ, Foot JZ, Reed KFM (1993) Perennial ryegrass (Lolium perenne) endophyte (Acremonium lolii) relationships: the Australian experience. Agriculture, Ecosystems & Environment 44, 157–168.
Perennial ryegrass (Lolium perenne) endophyte (Acremonium lolii) relationships: the Australian experience.Crossref | GoogleScholarGoogle Scholar |

Cunningham PJ, Blumenthal MJ, Anderson MW, Prakash KS, Leonforte A (1994) Perennial ryegrass improvement in Australia. New Zealand Journal of Agricultural Research 37, 295–310.
Perennial ryegrass improvement in Australia.Crossref | GoogleScholarGoogle Scholar |

Easton HS (2007) Grasses and Neotyphodium endophytes: co-adaptation and adaptive breeding. Euphytica 154, 295–306.
Grasses and Neotyphodium endophytes: co-adaptation and adaptive breeding.Crossref | GoogleScholarGoogle Scholar |

Easton HS, Lee CK, Fitzgerald RD (1994) Tall fescue in Australia and New Zealand. New Zealand Journal of Agricultural Research 37, 405–417.
Tall fescue in Australia and New Zealand.Crossref | GoogleScholarGoogle Scholar |

Edwards GR, Lucas RJ, Johnson MR (1993) Grazing preference for pasture species by sheep is affected by endophyte and nitrogen fertility. Proceedings of the New Zealand Grassland Association 55, 137–141.

Evans JW (2007) Commercialisation of AR1 Australia. In ‘Proceedings 6th International Symposium on Fungal Endophytes of Grasses’. Christchurch, New Zealand. Grassland Research and Practice Series No. 13. (Eds AJ Popay, ER Thom) pp. 241–242. (New Zealand Grassland Association: Dunedin, New Zealand)

Feely WF, Cameron F, Johnson M, Smith K, Anderson M (1994) South West Region. In ‘Pasture grasses and legumes ’94’. Research Report No. 150. (Eds SG Clark, FJ Cameron, LC Goss, AL Avery, RA Latta) pp. 17–24. (Department of Agriculture: Hamilton, Vic.)

Fletcher LR (2012) Novel endophytes in New Zealand grazing systems: The perfect solution or a compromise? In ‘Epichloae, endophytes of cool season grasses: Implications, utilization and biology’. (Eds CA Young, GE Aiken, RL McCulley, JR Strickland, CL Schardl) pp. 5–13. (The Samuel Roberts Noble Foundation: Ardmore, OK, USA)

Foot JZ, Brockhus M, Heazlewood PG, Saul GR, Reed KFM, Chin J, Cunningham PJ (1988) Productivity of sheep grazing perennial ryegrass pastures. In ‘Research review 1987/88. Pastoral Research Institute, Hamilton’. pp. 55–61. (Department of Agriculture and Rural Affairs: Hamilton, Vic.)

Foot JZ, Woodburn OJ, Walsh JR, Heazlewood PG (1994) Responses in grazing sheep to toxins from perennial ryegrass/endophyte associations. In ‘Plant–associated toxins—agricultural, phytochemical and ecological aspects’. (Eds SM Colgate, PR Dorling) pp. 375–380. (CAB International: Wallingford, UK)

Frost WE (1993) Role of the perennial ryegrass endophyte Acremonium lolii in population development of cereal rust mite. In ‘Proceedings 6th Australasian Conference on Grassland Invertebrate Ecology’. Hamilton, New Zealand. Vol. 6. (Ed. RA Prestidge) pp. 178–182. (AgResearch: Hamilton, New Zealand)

Gundel PE, Rudgers JA, Ghersa CM (2011) Incorporating the process of vertical transmission into understanding of host-symbiont dynamics. Oikos 120, 1121–1128.
Incorporating the process of vertical transmission into understanding of host-symbiont dynamics.Crossref | GoogleScholarGoogle Scholar |

Guy PL (1992) Incidence of Acremonium lolii and lack of correlation with barley yellow dwarf viruses in Tasmanian perennial ryegrass pastures. Plant Pathology 41, 29–34.
Incidence of Acremonium lolii and lack of correlation with barley yellow dwarf viruses in Tasmanian perennial ryegrass pastures.Crossref | GoogleScholarGoogle Scholar |

Guy PL, Davis LT (2002) Disease notes or new records: Variation in the incidence of Barley yellow dwarf virus and in the ability of Neotyphodium endophytes to deter feeding by aphids (Rhopalosiphum padi) on Australasian tall fescue. Australasian Plant Pathology 31, 307–308.
Disease notes or new records: Variation in the incidence of Barley yellow dwarf virus and in the ability of Neotyphodium endophytes to deter feeding by aphids (Rhopalosiphum padi) on Australasian tall fescue.Crossref | GoogleScholarGoogle Scholar |

Guy PL, Rowe BA (2002) Increased incidence of endophyte (Neotyphodium lolii) infected perennial ryegrass observed in Tasmanian pastures. The Tasmanian Naturalist 124, 35–37.

Harris CA, Clark SG, Reed KFM, Nie ZN, Smith KF (2008) Novel Festuca arundinacea Shreb. and Dactylis glomerata L. germplasm to improve adaptation for marginal environments. Australian Journal of Experimental Agriculture 48, 436–448.
Novel Festuca arundinacea Shreb. and Dactylis glomerata L. germplasm to improve adaptation for marginal environments.Crossref | GoogleScholarGoogle Scholar |

Hill MJ, Donald GE (1998) Determination of benefits from pasture improvement. Final report for Australian Meat Research Corporation. Revised edition. CSIRO Australia.

Hopkins AA, Young CA, Panaccione DG, Simpson WR, Mittal S, Bouton JH (2010) Agronomic performance and lamb health among several tall fescue novel endophyte combinations in the south-central USA. Crop Science 50, 1552–1561.
Agronomic performance and lamb health among several tall fescue novel endophyte combinations in the south-central USA.Crossref | GoogleScholarGoogle Scholar |

Hume DE, Barker DJ (2005) Growth and management of endophytic grasses in pastoral agriculture. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 201–226. (Blackwell Publishing: Ames, IA, USA)

Kahn L, Harris C, Lowien J (2003) Determining the presence of toxic alkaloids associated with endophytes of New England pastures. MLA PIRD Project (PIRD 2003/N07). BOZO Landcare Group. Meat & Livestock Australia, North Sydney.

Kimbeng CA, Tasneem M (1999) Incidence of ryegrass endophyte and resistance to barley yellow dwarf and ryegrass mosaic viruses among selected genotypes of perennial ryegrass. Annals of Applied Biology 134, 68–69.

Launders T, Blumenthal M, Kemp H (1996) Perennial ryegrass and tall fescue—evaluation. In ‘NSW Agriculture Dairy Research Institute 1995/96’. (Ed. W Fulkerson) pp. 15–20. (NSW Agriculture: Orange, NSW)

Lean IJ (2001) Association between feeding perennial ryegrass (Lolium perenne cultivar Grasslands Impact) containing high concentrations of ergovaline, and health and productivity in a herd of lactating dairy cows. Australian Veterinary Journal 79, 262–264.
Association between feeding perennial ryegrass (Lolium perenne cultivar Grasslands Impact) containing high concentrations of ergovaline, and health and productivity in a herd of lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXjtlGqtLo%3D&md5=7f14517fccee8a1d506f048a7cb2e82dCAS | 11349413PubMed |

Leuchtmann A (1993) Systematics, distribution, and host specificity of grass endophytes. Natural Toxins 1, 150–162.
Systematics, distribution, and host specificity of grass endophytes.Crossref | GoogleScholarGoogle Scholar |

Leuchtmann A, Bacon CW, Schardl CL, White JF Leuchtmann A, Bacon CW, Schardl CL, White JF (2014) Nomenclatural realignment of Neotyphodium species with genus Epichloë. Mycologia 106, 202–215.
Nomenclatural realignment of Neotyphodium species with genus Epichloë.Crossref | GoogleScholarGoogle Scholar | 24459125PubMed |

Lowe KF, Bowdler TM, Hume DE, Casey ND, Tapper BA (2008) The effect of endophyte on the performance of irrigated perennial ryegrasses in subtropical Australia. Australian Journal of Agricultural Research 59, 567–577.
The effect of endophyte on the performance of irrigated perennial ryegrasses in subtropical Australia.Crossref | GoogleScholarGoogle Scholar |

Malinowski DP, Belesky DP (2000) Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Science 40, 923–940.
Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXns1Gqsb8%3D&md5=935b3516230be8ab7c316a957fe3e9b3CAS |

McDonald G, Noske A, Van Heeswijck R, Frost WE (1993) The role of the perennial ryegrass endophyte in the management of pasture pests in south eastern Australia. In ‘Proceedings 6th Australasian Conference on Grassland Invertebrate Ecology’. Hamilton, New Zealand. Vol. 6. (Ed. RA Prestidge) pp. 122–128. (AgResearch: Hamilton, New Zealand)

Milne GD (2007) Technology transfer of novel ryegrass endophytes in New Zealand. In ‘Proceedings 6th International Symposium on Fungal Endophytes of Grasses’. Christchurch, New Zealand. Grassland Research and Practice Series No. 13. (Eds AJ Popay, ER Thom) pp. 237–239. (New Zealand Grassland Association: Dunedin, New Zealand)

Moate PJ, Williams SRO, Grainger C, Hannah MC, Mapleson D, Auldist MJ, Greenwood JS, Popay AJ, Hume DE, Mace WJ, Wales WJ (2012) Effects of wild-type, AR1 and AR37 endophyte-infected perennial ryegrass on dairy production in Victoria, Australia. Animal Production Science 52, 1117–1130.
Effects of wild-type, AR1 and AR37 endophyte-infected perennial ryegrass on dairy production in Victoria, Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhs1Wju7zL&md5=075b9c2dbc0a22cc3095f453362aefcaCAS |

Parish JA, McCann MA, Watson RH, Paiva NN, Hoveland CS, Parks AH, Upchurch BL, Hill NS, Bouton JH (2003) Use of nonergot alkaloid-producing endophytes for alleviating tall fescue toxicosis in stocker cattle. Journal of Animal Science 81, 2856–2868.

Popay AJ, Bonos SA (2005) Biotic responses in endophytic grasses. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 163–185. (Blackwell Publishing: Ames, IA, USA)

Popay AJ, Thom ER (2009) Endophyte effects on major insect pests in Waikato dairy pasture. In ‘Proceedings Pasture Persistence Symposium’. Hamilton, New Zealand. Grassland Research and Practice Series No. 15, Vol. 71. (Ed. CF Mercer) pp. 121–126. (New Zealand Grassland Association: Dunedin, New Zealand)

Popay AJ, Hume DE, Baltus JG, Latch GCM, Tapper BA, Lyons TB, Cooper BM, Pennell CG, Eerens JPJ, Marshall SL (1999) Field performance of perennial ryegrass (Lolium perenne) infected with toxin-free fungal endophytes (Neotyphodium spp.). In ‘Proceedings Ryegrass Endophyte: An Essential New Zealand Symbiosis Symposium’. Napier, New Zealand. Grassland Research and Practice Series No. 7. (Eds DR Woodfield, C Matthew) pp. 113–122. (New Zealand Grassland Association: Palmerston North, New Zealand)

Pulsford MF (1950) A note on lameness in cattle grazing on tall meadow fescue (Festuca arundinacea) in South Australia. Australian Veterinary Journal 26, 87–88.
A note on lameness in cattle grazing on tall meadow fescue (Festuca arundinacea) in South Australia.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaG3c%2FivFOmsA%3D%3D&md5=b4d0ee784f554ecb7e632ff2bc187075CAS | 15420120PubMed |

Quigley PE (2000) Effects of Neotyphodium lolii infection and sowing rate of perennial ryegrass (Lolium perenne) on the dynamics of ryegrass/subterranean clover (Trifolium subterraneum) swards. Australian Journal of Agricultural Research 51, 47–56.
Effects of Neotyphodium lolii infection and sowing rate of perennial ryegrass (Lolium perenne) on the dynamics of ryegrass/subterranean clover (Trifolium subterraneum) swards.Crossref | GoogleScholarGoogle Scholar |

van Heeswijck R, McDonald G (1992) Acremonium endophytes in perennial ryegrass and other pasture grasses in Australia and New Zealand. Australian Journal of Agricultural Research 43, 1683–1709.
Acremonium endophytes in perennial ryegrass and other pasture grasses in Australia and New Zealand.Crossref | GoogleScholarGoogle Scholar |

Reed KFM (1987) Perennial ryegrass in Victoria and the significance of the ryegrass endophyte. In ‘Proceedings Perennial Ryegrass without Staggers Symposium’. 2 April 1987, Mortlake, Vic. pp. 1–7. (Australian Institute of Agricultural Science and Australian Society of Animal Production: Parkville, Vic.)

Reed KFM (1996) Improving the adaptation of perennial ryegrass, tall fescue, phalaris, and cocksfoot for Australia. New Zealand Journal of Agricultural Research 39, 457–464.
Improving the adaptation of perennial ryegrass, tall fescue, phalaris, and cocksfoot for Australia.Crossref | GoogleScholarGoogle Scholar |

Reed KFM, Clark SG, Chin JF, Cunningham PJ, Mebalds M (1985) A phytotonic effect of endophyte on ryegrass establishment. In ‘Proceedings 3rd Australian Agronomy Conference’. January–February 1985, The University of Tasmania, Hobart, Tas. Vol. 3. (Ed. JJ Yates) p. 185. (Australian Society of Agronomy and Australian Institute of Agricultural Science: Parkville, Vic.)

Reed KFM, Cunningham PJ, Barrie JT, Chin JF (1987) Productivity and persistence of cultivars and Algerian introductions of perennial ryegrass (Lolium perenne L.) in Victoria. Australian Journal of Experimental Agriculture 27, 267–274.
Productivity and persistence of cultivars and Algerian introductions of perennial ryegrass (Lolium perenne L.) in Victoria.Crossref | GoogleScholarGoogle Scholar |

Reed KFM, Leonforte A, Cunningham PJ, Walsh JR, Allen DI, Johnstone GR, Kearney G (2000) Incidence of ryegrass endophyte (Neotyphodium lolii) and diversity of associated alkaloid concentrations among naturalised populations of perennial ryegrass (Lolium perenne L.). Australian Journal of Agricultural Research 51, 569–578.
Incidence of ryegrass endophyte (Neotyphodium lolii) and diversity of associated alkaloid concentrations among naturalised populations of perennial ryegrass (Lolium perenne L.).Crossref | GoogleScholarGoogle Scholar |

Reed KFM, Walsh JR, Cross PA, McFarlane NM, Sprague MA (2004) Ryegrass endophyte (Neotyphodium lolii) alkaloids and mineral concentrations in perennial ryegrass (Lolium perenne) from southwest Victorian pasture. Australian Journal of Experimental Agriculture 44, 1185–1194.
Ryegrass endophyte (Neotyphodium lolii) alkaloids and mineral concentrations in perennial ryegrass (Lolium perenne) from southwest Victorian pasture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXmsVOnsw%3D%3D&md5=c7946aab744aa1506d488e915bfb2eb1CAS |

Reed KFM, Page SW, Lean IJ (2005a) ‘Perennial ryegrass toxicosis in Australia.’ (Meat & Livestock Australia: North Sydney)

Reed KFM, Scrivener CJ, Rainsford KA, Walker LV (2005b) Neotyphodium research and application in Australia. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 43–54. (Blackwell Publishing: Ames, IA, USA)

Reed KFM, Nie ZN, Clark B (2008) Field evaluation of phalaris, tall fescue and cocksfoot cultivars and accessions in western Victoria, Australia. Australian Journal of Agricultural Research 59, 971–981.
Field evaluation of phalaris, tall fescue and cocksfoot cultivars and accessions in western Victoria, Australia.Crossref | GoogleScholarGoogle Scholar |

Reed KFM, Nie ZN, Walker LV, Mace WJ, Clark SG (2011) Weather and pasture characteristics associated with outbreaks of perennial ryegrass toxicosis in southern Australia. Animal Production Science 51, 738–752.
Weather and pasture characteristics associated with outbreaks of perennial ryegrass toxicosis in southern Australia.Crossref | GoogleScholarGoogle Scholar |

Sackett D, Francis J (2006) Economic assessment of the impact of wild endophyte-infected perennial ryegrass (Lolium perenne) on the productivity of sheep and cattle and the profitability of Australian livestock enterprises. Project No. AHW.089/B.AHW.0089. Meat & Livestock Australia, North Sydney.

Schardl CL, Leuchtmann A, Spiering MJ (2004) Symbioses of grasses with seedborne fungal endophytes. Annual Review of Plant Biology 55, 315–340.
Symbioses of grasses with seedborne fungal endophytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFeisL8%3D&md5=6b87418de30f0963e918108402ba9aeaCAS | 15377223PubMed |

Schmidt SP, Osborn TG (1993) Effects of endophyte-infected tall fescue on animal performance. Agriculture, Ecosystems & Environment 44, 233–262.
Effects of endophyte-infected tall fescue on animal performance.Crossref | GoogleScholarGoogle Scholar |

Sen LX (1995) Incidence and significance of fungal endophytes (Acremonium spp.) in selected grass species on the Northern Tablelands of NSW. Post Graduate Diploma in Science in Agriculture Thesis, University of New England, Armidale, NSW, Australia.

Thom ER, Popay AJ, Hume DE, Fletcher LR (2012) Evaluating the performance of endophytes in farm systems to improve farmer outcomes—a review. Crop & Pasture Science 63, 927–943.
Evaluating the performance of endophytes in farm systems to improve farmer outcomes—a review.Crossref | GoogleScholarGoogle Scholar |

Valentine SC, Bartsch BD, Boyce KG, Mathison MJ, Newbery TR (1993a) Incidence and significance of endophyte in perennial grass seed lines. Final report on project DAS33 prepared for the Dairy Research and Development Corporation. SARDI, Adelaide.

Valentine SC, Bartsch BD, Carroll PD (1993b) Production and composition of milk by dairy cows grazing high and low endophyte cultivars of perennial ryegrass. In ‘Proceedings Second International Symposium on Acremonium/Grass Interactions’. Vol. 1. (Eds DE Hume, GCM Latch, HS Easton) pp. 138–141. (AgResearch: Palmerston North, New Zealand)

Watson BM (2007) The effect of endophyte in perennial ryegrass and tall fescue on red and blackheaded pasture cockchafers. In ‘Proceedings 6th International Symposium on Fungal Endophytes of Grasses’. Christchurch, New Zealand. Grassland Research and Practice Series No. 13. (Eds AJ Popay, ER Thom) pp. 347–352. (New Zealand Grassland Association: Dunedin, New Zealand)

Welty RE, Craig AM, Azevedo MD (1994) Variability of ergovaline in seeds and straw and endophyte infection in seeds among endophyte-infected genotypes of tall fescue. Plant Disease 78, 845–849.
Variability of ergovaline in seeds and straw and endophyte infection in seeds among endophyte-infected genotypes of tall fescue.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXmvFOiurk%3D&md5=b64f82fc0abeae6963fa8271741dceaaCAS |

Wheatley WM (1997) Perennial ryegrass (Lolium perenne) staggers in the central tablelands, NSW, Australia. In ‘Proceedings Third International Symposium on Neotyphodium/Grass Interactions’. Athens, GA. (Eds CW Bacon, NS Hill) pp. 447–449. (Plenum Press: New York)

Wheatley W (2005a) Endophyte infected perennial ryegrass and tall fescue for New South Wales? In ‘Proceedings 20th Annual Conference of the Grassland Society of NSW’. 19–21 July 2005, Orange, NSW. Vol. 20, (Ed. HD Davies) pp. 38–43. (Grassland Society of NSW)

Wheatley WM (2005b) Endophytes, quality assurance and the seed trade in eastern Australia. In ‘Neotyphodium in cool-season grasses’. (Eds C Roberts, CP West, D Spiers) pp. 351–360. (Blackwell Publishing: Ames, IA, USA)

Wheatley WM (2009) The effect of perennial ryegrass (Lolium perenne) endophyte (Neotyphodium lolii) on grazing systems in the central tablelands of New South Wales. PhD Thesis, The University of Sydney, NSW, Australia.

Wheatley WM, Hume DE, Kemp HW, Monk MS, Lowe KF, Popay AJ, Baird DB, Tapper BA (2003) Effects of fungal endophyte on the persistence and productivity of tall fescue at 3 sites in eastern Australia. In ‘Solutions for a better environment. Proceedings 11th Australian Agronomy Conference’. (Australian Society of Agronomy/The Regional Institute: Gosford, NSW) Available at: www.regional.org.au/au/asa/2003/p/12/wheatley.htm

Wheatley WM, Kemp HW, Simpson WR, Hume DE, Nicol HI, Kemp DR, Launders TE (2007) Viability of endemic endophyte (Neotyphodium lolii) and perennial ryegrass (Lolium perenne) seed at retail and wholesale outlets in south-eastern Australia. Seed Science and Technology 35, 360–370.

Williams WM, Easton HS, Jones CS (2007) Future options and targets for pasture plant breeding in New Zealand. New Zealand Journal of Agricultural Research 50, 223–248.
Future options and targets for pasture plant breeding in New Zealand.Crossref | GoogleScholarGoogle Scholar |

Young CA, Hume DE, McCulley RL (2013) Fungal endophytes of tall fescue and perennial ryegrass: pasture friend or foe? Journal of Animal Science 91, 2379–2394.
Fungal endophytes of tall fescue and perennial ryegrass: pasture friend or foe?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXotl2gu7w%3D&md5=cdb20a32c6c5c711721cedffd33e4c02CAS | 23307839PubMed |