CSIRO PUBLISHING - Reproduction, Fertility and Development

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Role of the mitochondrial genome in preimplantation development and assisted reproductive technologies

Lawrence C. Smith, Jacob Thundathil and France Filion

pp. 15-22

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Messenger RNA expression patterns in bovine embryos derived from in vitro procedures and their implications for development

Christine Wrenzycki, Doris Herrmann, Andrea Lucas-Hahn, Karin Korsawe, Erika Lemme and Heiner Niemann

pp. 23-35

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Whole-genome approaches for large-scale gene identification and expression analysis in mammalian preimplantation embryos

James Adjaye

pp. 37-45

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Potential and limitations of bovine-specific arrays for the analysis of mRNA levels in early development: preliminary analysis using a bovine embryonic array

Marc-André Sirard, Isabelle Dufort, Maud Vallée, Lyne Massicotte, Catherine Gravel, Hélène Reghenas, Andrew J. Watson, W. Allan King and Claude Robert

pp. 47-57

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Commercial applications of nuclear transfer cloning: three examples

Erik J. Forsberg

pp. 59-68

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Mitochondria and the success of somatic cell nuclear transfer cloning: from nuclear–mitochondrial interactions to mitochondrial complementation and mitochondrial DNA recombination

Stefan Hiendleder, Valeri Zakhartchenko and Eckhard Wolf

pp. 69-83

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Telomere length regulation during cloning, embryogenesis and ageing

S. Schaetzlein and K. L. Rudolph

pp. 85-96

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Hand-made cloning approach: potentials and limitations

G. Vajta, P. M. Kragh, N. R. Mtango and H. Callesen

pp. 97-112

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Attempts towards derivation and establishment of bovine embryonic stem cell-like cultures

Jakob O. Gjørret and Poul Maddox-Hyttel

pp. 113-124

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From fibroblasts and stem cells: implications for cell therapies and somatic cloning

Wilfried A. Kues, Joseph W. Carnwath and Heiner Niemann

pp. 125-134

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Derivation characteristics and perspectives for mammalian pluripotential stem cells

Alan Trounson

pp. 135-141

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Comparison of neurosphere cells with cumulus cells after fusion with embryonic stem cells: reprogramming potential

Jeong Tae Do and Hans R. Schöler

pp. 143-149

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1 LONG TERM HEALTH AND BEHAVIOR OF ICSI PRODUCED MICE

R. Fernandez-Gonzalez, P. Moreira, A. Bilbao, M.A. Ramirez, M. Perez-Crespo, B. Pintado, F. Rodriguez de Fonseca and A. Gutierrez-Adan

pp. 151-151

2 CORRELATION BETWEEN OXYGEN RESPIRATION RATES AND MORPHOLOGY, SEX, DIAMETER AND DEVELOPMENTAL STAGE OF SINGLE BOVINE IVP-EMBRYOS

A.S. Lopes, N. Ramsing, L.H. Larsen, M. Räty, J. Peippo, T. Greve and H. Callesen

pp. 151-151

3 X-LINKED GENE EXPRESSION IN BOVINE PRE-IMPLANTATION EMBRYOS OBTAINED IN VIVO AND IN VITRO AS A MEASURE OF IMPACT OF EMBRYO PRODUCTION TECHNOLOGIES

M.I. Nino-Soto and W.A. King

pp. 152-152

4 EFFECT OF THE TIMING OF ARTIFICIAL INSEMINATION ON THE NUMBER OF SPERMATOZOA DISCOVERED IN THE UTERINE CRYPTS OF THE BITCH

T. Rijsselaere, A. Van Soom, S. Van Cruchten, M. Coryn, K. Gortz, D. Maes and A. de Kruif

pp. 152-152

5 NEUTRAL SEGREGATION OF DONOR CELL MITOCHONDRIA IN FETAL AND ADULT TISSUES OF SOMATIC CELL CLONES IN CATTLE

F. Viramontes, F. Filion and L.C. Smith

pp. 153-153

6 VITRIFICATION OF BOVINE EMBRYOS WITHOUT ANIMAL-DERIVED PRODUCTS

D. Walker and G. Seidel

pp. 153-153

7 HETEROSPERMIC INSEMINATION AT TWO SPERM CONCENTRATIONS IN TIMED AI: CASA SEMEN PARAMETERS AND PREGNANCY RATES

A. Albrecht, R. Cavia, G. Larraburu, F. Garcia Migliaro and G. Brogliatti

pp. 154-154

8 EFFECT OF SUCKLING RESTRICTION AND eCG TREATMENT ON PREGNANCY RATES IN POSTPARTUM BOS INDICUS CROSSBRED COWS TREATED WITH PROGESTERONE VAGINAL DEVICES AND ESTRADIOL BENZOATE

E. Balla, L. Cutaia, C. Rizzi, L. Peres and G.A. Bo

pp. 154-154

9 DOES STRAW CONCENTRATION AFFECT POST-THAW SPERM QUALITY IN AI BULL SIRES?

J. Ballester, A. Johannisson, M. Håård, H. Gustafsson and H. Rodriguez-Martinez

pp. 155-155

10 TIMED ARTIFICIAL INSEMINATION IN CATTLE WITH REDUCED DOSAGE OF SPERMATOZOA

F. Becker, H. Alm, F. Schneider, H. Nehring, L. Rothe and W. Kanitz

pp. 155-155

11 TIME OF INSEMINATION RELATIVE TO OVULATION EXPLAINS FERTILITY VARIATIONS OF FROZEN–THAWED SPERMATOZOA BETWEEN FARMS

A. Bolarín, G. Carvajal, M. Hernandez, J.M. Vazquez, E.A. Martinez and J. Roca

pp. 155-156

12 CASA PARAMETERS OF FRESH BULL SEMEN COLLECTED BY ARTIFICIAL VAGINA OR ELECTROEJACULATION IN ARGENTINA

G. Brogliatti, F. Garcia Migliaro, R. Cavia, G. Larraburu and A. Albrecht

pp. 156-156

13 EFFECTS OF PRESYNCHRONIZATION WITH A USED CIDR, AND TREATMENT WITH eCG ON FERTILITY IN LACTATING COWS SUBJECTED TO A COSYNCH PROTOCOL

M. Colazo, M. Rutledge, J. Small, J. Kastelic, L. Siqueira, D. Ward and R. Mapletoft

pp. 156-157

14 EFFECT OF TEMPORARY WEANING AND ECG TREATMENT ON PREGNANCY RATES IN POSTPARTUM BOS INDICUS COWS FOLLOWING TREATMENT WITH PROGESTERONE VAGINAL DEVICES AND ESTRADIOL BENZOATE

L. Cutaia, E. Balla, C. Rizzi, L. Peres and G.A. Bo

pp. 157-157

15 EFFECT OF SEMEN THAW METHOD ON CONCEPTION RATE IN DAIRY HEIFER HERDS

M. Kaproth, H. Rycroft, G. Gilbert, G. Abdel-Azim, B. Putnam, S. Schnell, R. Everett and J. Parks

pp. 157-158

16 A CIDR-BASED TIMED AI PROTOCOL RESULTED IN A HIGHLY ACCEPTABLE THERAPEUTIC OUTCOME ON OVARIAN FOLLICULAR CYSTS IN LACTATING DAIRY COWS

I.H. Kim, U.H. Kim and H.W. Nam

pp. 158-158

17 COMPARISON OF FOLLICULAR WAVE EMERGENCE AND FOLLICULAR DEVELOPMENT FOLLOWING ESTRADIOL BENZOATE PLUS PROGESTERONE OR GnRH AT THE FIRST FOLLICULAR WAVE IN A CIDR-TREATED, LACTATING HOLSTEIN COWS

U.H. Kim, G.H. Suh and I.H. Kim

pp. 158-159

18 STRATEGIES TO IMPROVE FERTILITY WITH COSYNCH-CIDR PROTOCOLS IN BEEF CATTLE

R. Mapletoft, M. Colazo, L. Siqueira, J. Small, M. Rutledge, D. Ward and J. Kastelic

pp. 159-159

19 LOW-DOSE DEEP INTRAUTERINE INSEMINATION IN SOWS UNDER CONDITIONS: INCIDENCE OF UNILATERAL FERTILIZATIONS

E.A. Martinez, J.M. Vazquez, I. Parrilla, C. Cuello, M.A. Gil, J.A. Tornel, J. Roca and J.L. Vazquez

pp. 159-160

20 EFFECTS OF DOSE OF ESTRADIOL BENZOATE AND PROGESTERONE IN PROSTAGLANDIN-TREATED BEEF HEIFERS

M. Martínez, M. Caccia, M. Colazo, G. Bó, J. Kastelic and R. Mapletoft

pp. 160-160

21 EFFECTS OF HETEROLOGOUS SEMEN PLASMA AND SEMEN EXTENDERS ON PROGRESSIVE MOTILITY OF FROZEN–THAWED RAM SPERM

G. Mataveia, S.J. Terblanche, J.O. Nöthling and D. Gerber

pp. 160-161

22 INFLUENCE OF SIRE AND SIRE BREED (GIR VS. HOLSTEIN) ON EARLY PREGNANCY AND EARLY EMBRYONIC LOSS RATES IN HOLSTEIN COWS DURING SUMMER HEAT STRESS

M.F. Pegorer, J.L.M. Vasconcelos, P.J. Hansen and C.M. Barros

pp. 161-161

23 PREGNANCY RATE IN NELORE COWS AFTER TEMPORARY CALF REMOVAL, AND USE OF HORMONAL PROTOCOLS WITH eCG

V. Pinheiro, A. Souza, R. Ereno and C. Barros

pp. 161-161

24 TRANSGENESIS AND NUCLEAR TRANSFER USING STEM CELLS FROM CULTURED PORCINE PRIMORDIAL GERM CELLS

K.S. Ahn, H.S. Yang, S.Y. Heo and H. Shim

pp. 162-162

25 IN VITRO DEVELOPMENT OF AGGREGATED NUCLEAR TRANSFERRED EMBRYOS DERIVED FROM BOVINE CUMULUS CELLS

S. Akagi, B. Tsuneishi, S. Watanabe and S. Takahashi

pp. 162-162

26 ASSESSMENT OF CHROMOSOME ABNORMALITIES IN SHEEP PARTHENOGENETIC AND NUCLEAR TRANSFER EMBRYOS: EFFECT OF 6-DMAP AND CYCLOHEXIMIDE ON PLOIDY

B. Alexander, G. Coppola, D. Di Berardino, D.H. Betts and W.A. King

pp. 163-163

27 MORPHOLOGICAL AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF DAY 21 IVP AND NT BOVINE EMBRYOS

N.I. Alexopoulos, P. Maddox-Hyttel, R.T. Tecirlioglu, M.A. Cooney and A.J. French

pp. 163-163

28 CLONED EMBRYOS CAN BE PRODUCED USING DONOR CELLS OBTAINED FROM A 72-HOUR COOLED CARCASS

S. Arat, H. Bagis, H. Odaman Mercan and A. Dinnyes

pp. 164-164

29 COMPARISON OF TERM PLACENTAS IN CLONED AND CONTROL PREGNANCIES IN CATTLE

C.A. Batchelder, M. Bertolini, K.A. Hoffert, J.B. Mason, A.L. Moyer, S.G. Petkov and G.B. Anderson

pp. 164-165

30. WITHDRAWN

31 CONSTRUCTION OF A TARGETING VECTOR SPECIFIC FOR THE BOVINE BETA-CASEIN GENE

M. Chang, K.-B. Oh, G. Wee, D.-B.n Koo, S.-T.e Shin, K.-K. Lee and Y.-M. Han

pp. 165-166

32 EFFICIENCY OF FEMALE-DERIVED DONOR CELLS ON HIGH POSTNATAL SURVIVAL IN PIG CLONING

S.K. Cho, M.R. Park, D.N. Kwon, E.K. Lee, S.J. Kang, S.Y. Lee, Y.J. Choi, J.Y. Park, W.J. Son and J.H. Kim

pp. 166-166

33 PRODUCTION OF TRANSGENIC-CLONED PIGS CARRYING HDAF, GnT-III AND HETEROZYGOUSLY DISRUPTED ±-1,3-GALACTOSYLTRANSFERASE GENES

T. Fujimura, Y. Takahagi, H. Nagashima, S. Miyagawa, T. Shigehisa and H. Murakami

pp. 166-166

34 LIFESPAN AND CHROMOSOMAL STABILITY OF BOVINE AND PORCINE FETAL FIBROBLAST CELLS CULTURED IN VITRO

A.M. Giraldo, J.W. Lynn, C.E. Pope, R.A. Godke and K.R. Bondioli

pp. 167-167

35 PRODUCTION OF CLONED MINIATURE CALVES USING CYTOPLASTS FROM COWS OF STANDARD SIZE

M. Givens, R. Carson, K. Riddell, D. Stringfellow, J. Waldrop, E. Whitley, P. Kasinathan and E. Sullivan

pp. 167-167

36 IMPROVING THE APPLICATION OF NUCLEAR TRANSFER FOR PRODUCING NON-DOMESTIC FELIDS

M.C. Gomez, C.E. Pope, L. Lyons, A. Cole, M. Lopez, C. Dumas and B.L. Dresser

pp. 168-168

37 PREGNANCY-ASSOCIATED GLYCOPROTEIN (PAG) PROFILES DURING THE PERI-IMPLANTATION PERIOD IN RECIPIENTS CARRYING BOVINE SOMATIC CLONES: PRELIMINARY RESULTS

Y. Heyman, P. Chavatte-Palmer, N. Melo de Sousa, F. Constant, M. Guillomot, X. Vignon and J.F. Beckers

pp. 168-169

38 HIGH OSMOLARITY AT EARLY CULTURE STAGE IMPROVES IN VITRO DEVELOPMENT OF PRE-IMPLANTATION PORCINE NUCLEAR TRANSFER EMBRYOS

G.-S. Im, B.-S. Yang, L. Lai, Z. Liu, Y. Hao and R.S. Prather

pp. 169-169

39 CHROMOSOME ABNORMALITIES IN BOVINE NUCLEAR TRANSFER EMBRYOS PRODUCED BY 'HANDMADE CLONING'

A.S. Jakobsen, G. Vajta, P.D. Thomsen, H. Callesen and T. Greve

pp. 169-169

40 CHARACTERIZATION OF EARLY G₁ CELLS AS NUCLEAR DONORS FOR SOMATIC CELL CLONING IN CATTLE

A. Kasamatsu, K. Saeki, T. Tamari, K. Shirouzu, S. Taniguchi, T. Mitani, Y. Aoyagi, M. Urakawa, A. Ideta, K. Matsumoto, Y. Hosoi and A. Iritani

pp. 170-170

41 EFFECT OF CELL TYPES AND PASSAGES ON DEVELOPMENT AND APOPTOSIS OF PORCINE CLONED EMBRYOS

J.-G. Kim, Y.-S. Lee, S.-L. Lee, S.-A. Ock, C.-S. Park, S.-Y. Choe and G.-J. Rho

pp. 170-170

42 PRODUCTION OF CLONED PIGS FROM SOMATIC STEM CELLS DERIVED FROM SALIVARY GLAND

M. Kurome, H. Ueda, R. Tomii, K. Nakamura, K. Okumura, S. Matsumoto, F. Endo and H. Nagashima

pp. 171-171

43 DNA SYNTHESIS, PREIMPLANTATION DEVELOPMENT AND Oct-4 EXPRESSION OF BOVINE CLONES RECONSTRUCTED WITH OOCYTES PREACTIVATED OR ENUCLEATED AFTER SPINDLE DISASSEMBLY

S. Kurosaka and K.J. McLaughlin

pp. 171-171

44 IMPROVED DEVELOPMENT OF BOVINE NUCLEAR TRANSFER EMBRYOS BY THE TREATMENT OF NUCLEAR DONOR CELLS WITH APOPTOSIS INHIBITORS DURING SERUM STARVATION

C.-K. Lee, Bon-sik Koo, C.-H. Park, S.-G. Lee, D.-H. Choi, J.-M. Lim and H.-J. Yong

pp. 171-172

45 ANALYSIS OF DIFFERENTIALLY EXPRESSED PROTEINS IN THE PLACENTA OF SOMATIC CELL CLONED AND ARTIFICIAL INSEMINATION PIG PLACENTA USING PROTEOMICS

S.Y. Lee, S.K. Cho, M.R. Park, E.K. Lee and J.H. Kim

pp. 172-172

46 EFFICIENT TRANSFECTION OF PLASMID DNA INTO CELLS FOR USE AS NUCLEAR DONORS

S.-L. Lee, S.-A. Ock, H.-J. Song, B. Mohana Kumar, S.-Y. Choe and G.-J. Rho

pp. 172-173

47 CHROMATIN AND CYTOSKELETAL REORGANIZATION OF RABBIT OOCYTES AFTER CUMULUS CELL NUCLEAR TRANSFER

C.-T. Liu, T.-A. Lin, P.-C. Tang and J.-C. Ju

pp. 173-173

48 PREGNANCY AND FETAL CHARACTERISTICS AFTER TRANSFER OF VITRIFIED IN VIVO AND CLONED BOVINE EMBRYOS

P. Lonergan, A.C.O. Evans, E. Boland, D. Rizos, L.-Y. Sung, F. Du, S. Chaubal, S. Fair, E. Scraggs, P. Duffy, J. Xu, X. Yang and X.C. Tian

pp. 173-174

49 EFFECT OF REPEATED CELL FREEZINGS ON PREGNANCY RATE OF BOVINE NUCLEAR TRANSFER DERIVED EMBRYOS

M. Marfil, M. Révora, J. Gutierrez, S. Sosa, J.J. Lagioia, M. Panarace and M. Medina

pp. 174-174

50 INVESTIGATION OF THE EFFECT OF BUTYROLACTONE I AND CYCLOHEXIMIDE TREATMENT DURING IN VITRO MATURATION OF SWINE OOCYTES

M.G. Marques, R.P.C. Gerger, A.B. Nascimento, V.P. Oliveira, R. Simoes, C.F. Lucio and J.A. Visintin

pp. 175-175

51 PHENOTYPIC VARIATION IN CLONED SWINE IS CORRECTED IN THE F1 GENERATION

B. Mir, G. Zaunbrecher and J.A. Piedrahita

pp. 175-175

52 NUCLEAR REMODELING AFTER SOMATIC CELL NUCLEAR TRANSFER (SCNT) IN THE RHESUS MONKEY

S. Mitalipov, J. Byrne, M. Sparman, C. Ramsey and D. Wolf

pp. 175-176

53 CELL CYCLE SYNCHRONIZATION OF DONOR CELLS AT G1 PHASE AND DEVELOPMENTAL ABILITY OF NUCLEAR TRANSFER EMBRYOS IN MINIATURE PIGS

K. Miyamoto, Y. Hoshino, Y. Nagao, N. Minami, M. Yamada and H. Imai

pp. 176-176

54 COMPOSITION OF ALLANTOIC FLUID IN CATTLE PREGNANT WITH AI-, IVP-, OR NUCLEAR TRANSFER-GENERATED EMBRYOS

C. Morrow, M. Berg, R. McDonald, D. Wells, A. Peterson and R. Lee

pp. 177-177

55 NUCLEUS CHANGES AND DEVELOPMENT OF PORCINE RECONSTRUCTED (NT) AND PARTHOGENETICALLY ACTIVATED (PA) EMBRYOS

N.R. Mtango and T. Kono

pp. 177-177

56 SOMATIC CELL NUCLEAR TRANSFER IN NON-HUMAN PRIMATES: THE POSSIBILITY OF USING OOCYTES MATURED IN VITRO FOR UP TO 3 DAYS AS HOST OOPLASTS

N.T. Uoc, B.D. Bavister, N.V. Hanh, L.C. Bui, N.T. Thanh, N.H. Duc, Q.X. Huu, N.V. Linh and X.N. Bui

pp. 177-178

57 INVESTIGATION OF CYNOMOLGUS MONKEY (MACACA FASCICULARIS) FETUS FIBROBLAST CELL NUCLEAR TRANSFER

J. Narita, H. Tsuchiya, T. Takada and R. Torii

pp. 178-178

58 HANDMADE CLONING IN TRANS-SPECIES NT: CULTURE MEDIUM HAS AN EFFECT ON THE ABILITY OF RECONSTRUCTED BOVINE-MURINE EMBRYOS TO DEVELOP BEYOND THE 8-CELL STAGE

P.-M. Nieminen, M. Aho, K. Kananen-Anttila, E. Reinikainen and M. Halmekytö

pp. 179-179

59 A PRELIMINARY STUDY OF THE IN VITRO DEVELOPMENT OF ASIAN ELEPHANT, CLONED EMBRYOS, RECONSTRUCTED USING A RABBIT RECIPIENT OOCYTE

P. Numchaisrika, R. Rungsiwiwut, A. Thongpakdee and M. Techakumphu

pp. 179-180

60 HISTOLOGICAL COMPARISONS BETWEEN NUCLEAR TRANSFER AND IN VIVO PORCINE EMBRYOS

L. Overman, L. Lai, H.-T. Cheong, G.-S. Im, K.-W. Park, C. Besch-Williford, C. Murphy, J. Green and R. Prather

pp. 180-180

61 CLONED MOUSE PRODUCED USING A ZONA FREE METHOD OF NUCLEAR TRANSFER

R. Ribas, B. Oback, J. Taylor, A. Maurício, M. Sousa and I. Wilmut

pp. 180-181

62 RELATION OF INTENSITY OF GENE EXPRESSION IN BOVINE RECONSTRUCTED EMBRYOS TO SUBSEQUENT DEVELOPMENT

K. Saeki, T. Tamari, A. Kasamatsu, K. Shirouzu, S. Taniguchi, K. Matsumoto, Y. Hosoi and A. Iritani

pp. 181-181

63 IMPROVED IN VITRO DEVELOPMENT OF PORCINE EMBRYOS PRODUCED BY NUCLEAR TRANSFER, IVF AND PARTHENOGENESIS

D. Sage, P. Hassel, B. Petersen, W. Mysegades, P. Westermann, A. Lucas-Hahn and H. Niemann

pp. 181-182

64 OOPLASMIC TRANSFER AFTER INTERSPECIES NUCLEAR TRANSFER: PRESENCE OF FOREIGN MITOCHONDRIA, PATTERN OF MIGRATION, AND EFFECT ON EMBRYO DEVELOPMENT

M. Sansinena, J. Lynn, R. Denniston and R. Godke

pp. 182-182

65 PRODUCTION OF PORCINE NUCLEAR TRANSFER EMBRYOS USING FETAL FIBROBLAST CELLS ANALYZED ON APOPTOSIS

M. Skrzyszowska and M. Samiec

pp. 182-183

66 IN VITRO DEVELOPMENT OF YAK (POEPHAGUS MUTUS) CLONED EMBRYOS BY INTERSPECIES SOMATIC NUCLEAR TRANSFER

L. Su, F.L. Du, L.Y. Sung, S. Yang, B.S. Jeong, S. Casinghino, T.L. Nedambale, J. Xu, C.X. Tian, X. Yang and W. Ji

pp. 183-183

67 PREMATURE CHROMOSOME CONDENSATION IS NOT ESSENTIAL FOR BOVINE SOMATIC NUCLEAR REPROGRAMMING

L.Y. Sung, F. Du, B.S. Jeong, C.C. Chang, J. Xu, X.C. Tian and X. Yang

pp. 183-184

68 REPRODUCTIVE PERFORMANCE OF CLONED BULLS

R.T. Tecirlioglu, M.A. Cooney, N.A. Korfiatis, R. Hodgson, M. Williamson, S. Downie, D.B. Galloway, M.K. Holland and A.J. French

pp. 184-184

69 PRODUCTION OF CLONED PIGS BY NUCLEAR TRANSFER OF PREADIPOCYTES

R. Tomii, M. Kurome, H. Ueda, S. Ueno, K. Hiruma, K. Kano and H. Nagashima

pp. 184-185

70 RECONSTRUCTED BOVINE BLASTOCYSTS COMPRISING NUCLEAR TRANSFER-DERIVED INNER CELL MASS AND TROPHECTODERM FROM IVF EMBRYOS DO NOT IMPROVE IN VIVO DEVELOPMENT OF CLONES

H.E. Troskie, F.C. Tucker, M.C. Berg, B. Oback, D.N. Wells and R.S.F. Lee

pp. 185-185

71 DEVELOPMENTAL DELAY OF PRE-IMPLANTATION OVINE IN VITRO CULTURED AND SOMATIC CELL NUCLEAR TRANSFER EMBRYOS

P. Tveden-Nyborg, T. Peura, K. Hartwich and P. Maddox-Hyttel

pp. 185-186

72 EFFECT OF CELL CYCLE PHASE OF GENE-MANIPULATED FETAL FIBROBLASTS ON THE DEVELOPMENT OF CLONED BOVINE EMBRYOS

M. Urakawa, T. Sawada, Y. Sendai, Y. Shinkai, A. Ideta, K. Kubota, H. Hoshi and Y. Aoyagi

pp. 186-186

73 ABNORMAL REPROGRAMMING OF HISTONE ACETYLATION IN CLONED BOVINE EMBRYOS

G. Wee, D.-B. Koo, M.-J. Kang, S.J. Moon, K.-K. Lee and Y.-M. Han

pp. 186-187

74 ANATOMICAL ABNORMALITIES IN CALVES PRODUCED BY NUCLEAR TRANSFER

M. Williamson, R. Tecirlioglu, A. French and M. Holland

pp. 187-187

75 RABBIT NUCLEAR TRANSFER WITH CULTURED SOMATIC CELLS

F. Yang, B. Kessler, S. Ewerling, E. Wolf and V. Zakhartchenko

pp. 187-187

76 PRODUCTION OF CLONED BOVINE TRANSGENIC EMBRYOS WITH VARIOUS TYPES OF MONO-COLONY CELLS AND OVUM PICKUP

J.G. Zhao, X.Y. Yang, Y. Huang, H.F. Liu, H. Li, H.W. Li, S.Z. Huang and Y.T. Zeng

pp. 188-188

77 FREEZING OF MOUSE SPERM BY THREE DIFFERENT CRYOPROTECTANTS

C.S. An, S.J. Uhm, D.H. Ko, H.M. Chung, S.-G. Cho, K.S. Chung and H.T. Lee

pp. 188-188

78 SURVIVAL OF PORCINE OOCYTES AT GERMINAL VESICLE STAGE AFTER VITRIFICATION WITH OPEN PULLED STRAW METHOD

A. Bali Papp, T. Somfai, E. Varga and M. Marosán

pp. 189-189

79 EFFECT OF PRE-FREEZE ADDITION OF PLATELET-ACTIVATING FACTOR AND PLATELET-ACTIVATING FACTOR:ACETYLHYDROLASE ON THE POST-THAW INTEGRITY OF FROZEN–THAWED BOAR SPERM

R. Bathgate, B.M. Eriksson, W.M.C. Maxwell and G. Evans

pp. 189-189

80 GENE EXPRESSION PROFILES AND IN VITRO DEVELOPMENT FOLLOWING VITRIFICATION OF PRONUCLEAR AND 8 CELL-STAGE MOUSE EMBRYOS

D. Boonkusol, A. Baji Gal, S.Z. Bodo, B. Gorhony, K. Pavasuthipaisit and A. Dinnyes

pp. 190-190

81 EFFECT OF SUCROSE CONCENTRATION IN WARMING MEDIUM ON THE DEVELOPMENT POTENTIAL OF VITRIFIED BOVINE OOCYTES

W.C. Chang, J. Xu, S. Jiang, X.C. Tian, X. Yang and F.L. Du

pp. 190-191

82 EVALUATION OF CELL DEATH IN CRYOPRESERVED MOUSE EMBRYOS

A.R.S. Coutinho, A.B. Nascimeto, C.M. Mendes, R. Simoes, C.F. Lucio, M.L.Z. Dagli and M.E.O.A. Assumpcao

pp. 191-191

83 CRYOPRESERVATION OF IN VITRO PORCINE OOCYTES BY SOLID SURFACE VITRIFICATION (SSV)

A. Dinnyes, T. Somfai, D. Sage, M. Marosan, J. Carnwath and H. Niemann

pp. 191-192

84 SUCCESSFUL CRYOPRESERVATION OF PORCINE EMBRYOS BY THE METAL MESH VITRIFICATION (MMV) METHOD

Y. Fujino and Y. Nakamura

pp. 192-192

85 ADDITION OF REDUCED GLUTATHIONE TO FREEZING MEDIUM IMPROVED THE SPERM FUNCTIONALITY OF THAWED BOAR SPERMATOZOA

J. Gadea, F. Garcia-Vázquez, D. Gumbao, F.A. Rodríguez and C. Matás

pp. 192-193

86 CULTURE OF PIG EMBRYOS BEFORE CRYOPRESERVATION

B. Gajda and Z. Smorag

pp. 193-193

87 VITRIFICATION OF BOVINE OOCYTES TREATED WITH CHOLESTEROL-LOADED METHYL-β-CYCLODEXTRIN

G. Horvath, L. Solti and G. Seidel

pp. 193-194

88 PREGNANCY RATES FOR IN VITRO AND IN VIVO PRODUCED OVINE EMBRYOS VITRIFIED USING THE MINIMUM VOLUME COOLING CRYOTOP METHOD

J. Kelly, D. Kleemann, M. Kuwayama and S. Walker

pp. 194-194

89 EVALUATION OF A CUSHIONED CENTRIFUGATION TECHNIQUE FOR PROCESSING BOAR SEMEN FOR FREEZING

C. Matas, J. Gadea and G. Decuadro-Hansen

pp. 195-195

90 PRESERVATION OF HERITAGE LIVESTOCK BREEDS: INTEGRATED PROGRAM TO CRYOPRESERVE GERMPLASM FROM TENNESSEE MYOTONIC GOATS

D. Matsas, V. Huntress, H. Levine, S. Ayres, J. Amini, R. Duby, P. Borden, G. Saperstein and E. Overstrom

pp. 195-195

91 EMBRYO DEVELOPMENT AFTER ICSI OF EQUINE OOCYTES VITRIFIED BEFORE AND AFTER IVM

B. Merlo, E. Iacono, S. Colleoni, E. Dell'Aquila, C. Galli and G. Mari

pp. 195-196

92 BLASTOCYST FORMATION FROM VITRIFIED BOVINE OOCYTES, ZYGOTES, AND TWO-CELL EMBRYOS

A. Moisan, E. Chamberlain, S. Leibo, B. Dresser, K. Bondioli and R. Godke

pp. 196-196

93 BLASTOCYST PRODUCTION FROM BOVINE OOCYTES VITRIFIED IN A CLOSED (BIOSECURE) SYSTEM FOLLOWING IN VITRO MATURATION IN THE PRESENCE OR ABSENCE OF VITAMIN E

V.C. Moreira, G.J. McCallum, A. Ainslie and T.G. McEvoy

pp. 196-197

94 COMPARING DIFFERENT LEVELS OF OSMOLARITY AND pH OF LACTOSE EXTENDER ON THE VIABILITY OF SPERMATOZOA IN THE BACTRIAN CAMEL (CAMELUS BACTRIANUS)

S. Mosaferi, A. Niasari-Naslaji, A.A. Gharahdaghi, A. Abarghani, A. Ghanbari and A. Gerami

pp. 197-197

95 EFFECT OF ESTROUS COW SERUM ON SURVIVAL OF IN VITRO-PRODUCED BOVINE EMBRYOS AFTER SLOW FREEZING OR VITRIFICATION

N. Mucci, J. Aller, P. Ross, G. Kaiser, J. Cabodevila, F. Hozbor and R. Alberio

pp. 198-198

96 A NOVEL EXTENDER FOR PRESERVATION OF BACTRIAN CAMEL (CAMELUS BACTRIANUS) SEMEN

A. Niasari-Naslaji, S. Mosaferi, A.A. Gharahdaghi, A. Abarghani, A. Ghanbari and A. Gerami

pp. 198-199

97 ASSESSMENT OF VIABILITY OF IN VITRO PRODUCED BOVINE EMBRYOS FOLLOWING VITRIFICATION BY CVM OR SLOW FREEZING WITH ETHYLENE GLYCOL AND TRIPLE TRANSFER

B. Peachey, K. Hartwich, K. Cockrem, A. Marsh, A. Pugh, J. van Wagtendonk and W. Lindemans

pp. 199-199

98 THE EFFECT OF HIGH HYDROSTATIC PRESSURE ON THE MOTILITY OF FRESH AND FROZEN-THAWED BULL SEMEN

C.S. Pribenszky, M. Molnar, L. Solti, J. Dengg and J. Lederer

pp. 199-200

99 COMPARISON OF IN VITRO DEVELOPMENT FOLLOWING CRYOPRESERVATION OF MEISHAN AND WHITE CROSS SWINE EMBRYOS

B. White, M. Montagner, G. Mills, P. Gonçalves and R. Christenson

pp. 200-200

100 DIET AND FATTY ACID COMPOSITION OF BOVINE PLASMA, GRANULOSA CELLS, AND CUMULUS–OOCYTE COMPLEXES

S. Adamiak, M. Ewen, J. Rooke, R. Webb and K. Sinclair

pp. 200-201

101 OVARIAN RESPONSE AND DEVELOPMENTAL COMPETENCE OF OOCYTES COLLECTED BY OPU IN SHEEP TREATED WITH GnRH ANTAGONIST

F. Berlinguer, A. Gonzalez-Bulnes, S. Succu, G. Leoni, I. Rosati, A. Veiga-Lopez, R.M. Garcia Garcia, M.J. Cocero and S. Naitana

pp. 201-201

102 ANALYSIS OF EARLY EMBRYONIC TRANSCRIPTION IN THE BOVINE EMBRYO USING A DEDICATED cDNA LIBRARY

L.C. Bui, C. Faure, X. Vignon, J.P. Renard and V. Duranthon

pp. 202-202

103 GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IS DISPENSIBLE DURING REGULATION OF TIGHT JUNCTION MEMBRANE ASSEMBLY BY CELL CONTACT PATTERN AND PKC SIGNALING

J. Eckert, A. Mears, I. Cameron and T. Fleming

pp. 202-202

104 EFFECT OF FROZEN MEDIA ON IGF₂ EXPRESSION OF BOVINE EMBRYOS CULTURED ENTIRELY IN VITRO UNTIL DAY 14

M.M. Franco, D.O. Brandão, D.C. Pereira, T.C.D. Mundim, F.F. Ávila, E.O. Melo, M.A.N. Dode and R. Rumpf

pp. 203-203

105 EXPRESSION OF PLURIPOTENCY-DETERMINING FACTORS Oct-4 AND NANOG IN PRE-IMPLANTATION GOAT EMBRYOS

S. He, D. Pant, S. Bischoff, W. Gavin, D. Melican and C. Keefer

pp. 203-203

106 GENOMIC IMPRINTING OF IGF2R IN TISSUES OF BOVINE FETUSES GENERATED BY ARTIFICIAL INSEMINATION OR IN VITRO FERTILIZATION

S. Hiendleder, D. Bebbere, S. Bauersachs, M. Stojkovic, H. Wenigerkind, H.-D. Reichenbach, S. Ledda and E. Wolf

pp. 204-204

107 INITIAL RESULTS FROM MALE GERM CELL TRANSFER BETWEEN CATTLE BREEDS

J. Hill, A. Brownlee, R. Davey, M. Herrid, K. Hutton, S. Vignarajan and I. Dobrinski

pp. 204-204

108 THE ROLE OF NITRIC OXIDE SYNTHASE IN IN VITRO DEVELOPMENT OF BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS

A.K. Kadanga, D. Tesfaye, S. Ponsuksili, K. Wimmers, M. Gilles and K. Schellander

pp. 204-205

109 A BRIDGE OF SPERM TAIL BETWEEN BLASTOMERES ENHANCED PROTEIN MIGRATION IN THE RAT TWO-CELL STAGE EMBRYOS

N. Kashiwazaki, A. Takizawa, S. Hisamatsu, Y. Akamatsu, M. Shino, S. Sonoki and T. Inomata

pp. 205-205

110 NUCLEAR LAMIN A/C EXPRESSION IN BOVINE IVF EMBRYOS

R.D.W. Kelly, R. Alberio and K.H.S. Campbell

pp. 205-206

111 CLONING AND CHARACTERIZATION OF PIG VASA HOMOLOG GENE AND ITS SPECIFIC EXPRESSION IN GERM CELL LINEAGE

G.S. Lee, S.H. Lee, H.S. Kim, E.B. Jeung, S.K. Kang, B.C. Lee and W.S. Hwang

pp. 206-206

112 THE PRESENCE OF LAMIN A/C ANTIGENS IN PORCINE EMBRYOS

K. Lee, W.L. Fodor and Z. Machaty

pp. 206-207

113 THE DISTRIBUTION OF THE LEPTIN PROTEIN WITHIN BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS MATURED AND FERTILIZED IN VITRO

Z. Madeja, D. Lechniak, J. Peippo and M. Switonski

pp. 207-207

114 PORCINE EMBRYO FRAGMENTATION, DEVELOPMENT AND APOPTOSIS: A CONFOCAL MICROSCOPY STUDY

B. Mateusen, A. Van Soom, D. Maes and A. de Kruif

pp. 207-207

115 CYTOLOGICAL ANALYSIS OF HEPATIC GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE OF MHC ANTIGENS IN MOUSE AMNIOTIC EPITHELIAL CELLS

T. Mitani, T. Nagai, D. Suzuki, Y. Ukida, H. Kato, K. Matsumoto, K. Saeki, Y. Hosoi and A. Iritani

pp. 208-208

116 ACTIVE METHYLATION AND ACETYLATION OF HISTONE H3-K9 IN MOUSE EMBRYO WITH DIFFERENT PROPORTIONS OF MATERNAL AND PATERNAL GENOME

V.T. Nguyen, S. Wakayama, H.-T. Bui and T. Wakayama

pp. 208-208

117 CYTOPLASMIC FACTORS INFLUENCE DEVELOPMENTAL POTENTIAL OF SAMP1/Yit MOUSE EMBRYOS

J. Otsuka, H. Funabashi and T. Kono

pp. 209-209

118 TRANSFER OF IRON FROM MOTHER TO FETUS IN WATER BUFFALO: ERYTROPHAGOCYTOSIS AND UTEROFERRIN

F. Pereira, F. Braga, J.R. Kfoury Jr, L. Oliveira, P. Papa, A.F. Carvalho, A. Kohayagawa, W. Hamlett, S. Oloris, M.L. Dagli, F. Bazer and M.A. Miglino

pp. 209-210

119 THE LOCALIZATION OF A METHYL BINDING DOMAIN PROTEIN (MBD4) IN MURINE AND BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS

N. Ruddock, J. Xue, L. Sanchez-Partida, M. Cooney, N. Korfiatis and M. Holland

pp. 210-210

120 EMBRYONIC AND POSTNATAL DEVELOPMENT OF DIPLOID–TRIPLOID MOUSE CHIMAERAS

A. Suwinska, M. Waksmundzka, W. Ozdzenski and A.K. Tarkowski

pp. 210-211

121 HEAT SHOCK TO PIG OOCYTES DOES NOT INDUCE APOPTOSIS BUT REDUCES EMBRYO DEVELOPMENT

J.-K. Tseng, P.-C. Tang and J.-C. Ju

pp. 211-211

122 USE OF A DAY-14 EMBRYONIC ARRAY TO STUDY THE ELONGATION PHASE OF THE BOVINE EMBRYO

S. Degrelle, I. Hue and J.P. Renard

pp. 211-212

123 PROGESTERONE LEVELS DURING 20 DAYS OF PREGNANCY IN RABBIT TREATED FOR ENDOMETRIOSIS OR WITH ANTI-CD44

M.J. Illera, P. Bermejo, A. Natarajan, C. Willingham and J. Hernandez

pp. 212-212

124 THE MAJOR GENE EXPRESSION PATTERNS IN ENDOMETRIAL TISSUE OF PIGS DURING EARLY GESTATION

B.-K. Kim, H.J. Chung, Y.G. Ko, Y.M. Kim, H.-H. Seong, J.K. Jung, N.-H. Kim and W.K. Chang

pp. 212-213

125 CHANGES OF PLASMA MACROPHAGE COLONY-STIMULATING FACTOR LEVELS AND ITS GENE EXPRESSION IN PERIPHERAL WHITE BLOOD CELLS DURING PREGNANCY IN JAPANESE BLACK CATTLE

K. Oshima, K. Yoshihara, H. Watanabe, T. Kojima, M. Komatsu and N. Yamamoto

pp. 213-213

126 EFFECTS OF THE REPRODUCTIVE STATUS ON DEVELOPMENTAL COMPETENCE OF RECIPIENT OOCYTES AFTER SOMATIC CELL NUCLEAR TRANSFER IN CAT

T. Otoi, N.W.K. Karja, M. Fahrudin, B. Agung and P. Wongsrikeao

pp. 213-214

127 VILLOUS ARCHITECTURE AND FETO-MATERNAL INTERDIGITATION IN THE AFRICAN BUFFALO (SYNCERUS CAFFER) DURING DIFFERENT GESTATION STAGES

S. Schmidt, J.T. Soley, T.A. Aire and D. Gerber

pp. 214-214

128 SERUM TRANSAMINASE ACTIVITIES IN ENDOMETRITIC, REPEAT BREEDER AND ESTRUS CROSSBRED COWS

K.D. Tareen, M.A. Kakar, Z.I. Qureshi, L.A. Lodhi and H.A. Samad

pp. 214-215

129 RESPIRATION ACTIVITY OF BOVINE EMBRYOS CULTURED IN SERUM-FREE AND SERUM-CONTAINING MEDIA

H. Abe, H. Shiku, S. Aoyagi, T. Matsue and H. Hoshi

pp. 215-215

130 THE EFFECT OF ALTERED ENERGY SUBSTRATE CONCENTRATIONS ON THE DEVELOPMENT OF DIPLOID PARTHENOGENETIC PORCINE EMBRYOS CREATED FROM OOCYTES FROM GILTS AND SOWS

L. Beebe, S. McIlfactrick and M. Nottle

pp. 215-216

131 MODIFICATION OF AMINO ACID CONCENTRATIONS IN MEDIUM BY PIG EMBRYOS FROM THE ZYGOTE TO THE BLASTOCYST STAGE

P. Booth, T. Watson and H. Leese

pp. 216-216

132 BLASTOCYST DEVELOPMENT OF EQUINE OOCYTES WITH LOW MEIOTIC COMPETENCE HELD IN ROSCOVITINE BEFORE IN VITRO MATURATION

Y.H. Choi, L.B. Love, D.D. Varner and K. Hinrichs

pp. 216-217

133 INSULIN, TRANSFERRIN AND SELENIUM WITH OR WITHOUT BSA IN A SERUM-FREE CULTURE SYSTEM FOR BOVINE EMBRYO, AND ITS SUITABILITY FOR EMBRYOS CULTURED IN SMALL GROUPS

C. Daniaux, B. Verhaeghe and I. Donnay

pp. 217-218

134 EVALUATION OF APOPTOSIS IN BOVINE EMBRYOS BY FLUORESCENT LABELING OF CASPASES-3 AND -7

T.R. Davidson, C.E. Ferguson, J. Lynn, T. Chapman, K. Hebert, M.B. Wheeler, K. Bondioli and R.A. Godke

pp. 217-218

135 REGULATION OF GLUCOSE METABOLISM TO DECREASE LIPID CONTENT OF IN VIRTO-PRODUCED BOVINE EMBRYOS

J. De La Torre-Sanchez, D. Gardner, K. Preis and G. Seidel Jr

pp. 218-218

136 EVIDENCE OF A DIRECT EFFECT OF P₄ ON IVF-DERIVED BOVINE 8-CELL EMBRYOS

C.E. Ferguson, T.R. Davidson, M.R.B. Mello, A.S. Lima, D.J. Kesler, M.B. Wheeler and R.A. Godke

pp. 219-219

137 EFFECT OF FLUNIXIN MEGLUMINE IN CO-CULTURE MEDIUM ON THE DEVELOPMENT OF IN VITRO MATURED AND FERTILIZED BOVINE EMBRYOS

S. Goda, S. Hamano, M. Miyamura, O. Dochi and H. Koyama

pp. 219-219

138 THE EFFECT OF SYNTHETIC HYALURONAN, BSA AND SERUM ON IN VITRO DEVELOPMENT AND GENE TRANSCRIPTION OF BOVINE BLASTOCYSTS

A. Gutiérrez-Adán, H. Rodriguez-Martinez, P. Beltrán Breña, J. De la Fuente and A.T. Palasz

pp. 219-220

139 BOVINE AMNIOTIC FLUID FOR THE CULTURE OF TWO-CELL MURINE EMBRYOS

G.J.M. Herholdt and D.M. Barry

pp. 220-220

140 EXPRESSION OF LEPTIN LIGAND AND RECEPTOR AND EFFECT OF EXOGENOUS LEPTIN SUPPLEMENTATION ON IN VITRO DEVELOPMENT OF PORCINE IN VITRO FERTILIZED AND SOMATIC CELL NUCLEAR TRANSFER EMBRYOS

H.S. Kim, G.S. Lee, J.H. Kim, S.K. Kang, B.C. Lee and W.S. Hwang

pp. 220-221

141 BENEFICIAL EFFECT OF ETHYLENEDIAMINETETRAACETIC ACID COMBINED WITH HEMOGLOBIN ON PRE-IMPLANTATION DEVELOPMENT OF PORCINE IN VITRO PRODUCED EMBRYOS

J.H. Kim, G.S. Lee, H.S. Kim, S.H. Lee, D.H. Nam, Y.W. Jeong, Y.S. Kwon, S. Kim, S.W. Park, S.K. Kang, B.C. Lee and W.S. Hwang

pp. 221-221

142 IN VITRO DEVELOPMENT OF BOVINE EMBRYOS CULTURED IN KSOM, CR1AA, OR KSOM/CR1aa

M.R.B. Mello, C.E. Ferguson, A.S. Lima and M.B. Wheeler

pp. 221-222

143 IN VITRO DEVELOPMENT OF OVINE OOCYTES FROM EWES OF CONTRASTING VITAMIN B12 STATUS

L.M. Mitchell, G. McCallum, K. Mackie, M. Ewen, A. Ainslie and T.G. McEvoy

pp. 222-222

144 ROLE OF GnRH ON MOUSE PRE-IMPLANTATION EMBRYONIC DEVELOPMENT IN VITRO

M. Montagner, A. Cropp, J. Swanson, R. Cederberg, P. Goncalves and B. White

pp. 222-223

145 COMPARISON OF DEVELOPMENT AND QUALITY OF PORCINE EMBRYOS CULTURED IN DIFFERENT OXYGEN CONCENTRATIONS

S.-A. Ock, J.-G. Kim, L.-Y. Shi, H.-F. Jin, B. Mohana Kumar, S.-Y. Choe and G.-J. Rho

pp. 223-223

146 THE EFFECT OF CULTURE TEMPERATURE ON THE CLEAVAGE, DEVELOPMENT, AND GENE TRANSCRIPTION PATTERNS OF BOVINE EMBRYOS

A.T. Palasz, P. Beltrán Breña, M.A. Ramírez, S. Pérez-Garnelo, A. Gutiérrez-Adán and J. De la Fuente

pp. 223-224

147 EFFECT OF CULTURE SYSTEM ON THE DEVELOPMENT OF IN VITRO-FERTILIZED OR DNA-INJECTED BOVINE EMBRYOS

Y.S. Park and G.S. Min

pp. 224-224

148 AN EFFECT OF MELATONIN ON DEVELOPMENT OF BOVINE EMBRYOS CULTURED IN VITRO UNDER OPTIMAL OR ENHANCED OXYGEN TENSIONS

O. Poleszczuk, K. Papis and E. Wenta-Muchalska

pp. 224-224

149 INTRAUTERINE CULTURE OF IN VITRO PRODUCED BOVINE EMBRYOS AND RECOVERY OF THE EMBRYOS AT DAYS 12–14

M. Schmidt, B. Avery and T. Greve

pp. 225-225

150 ENVIRONMENT OF THE EARLY EMBRYO AND ITS EFFECT ON DEVELOPMENT AND POSTNATAL LIFE

A. Watkins, A. Wilkins, T. Papenbrock, C. Osmond, M. Hanson and T. Fleming

pp. 225-226

151 EFFECTS OF HEXOSES SUPPLEMENTED IN THE MATURATION AND PRE IMPLANTATION MEDIUM ON THE IN VITRO DEVELOPMENT OF PORCINE OOCYTES

P. Wongsrikeao, A. Budiyanto, R. Ooki, M. Teniguchi and T. Otoi

pp. 226-226

152 IN VITRO AND IN VIVO DEVELOPMENT OF BOVINE IVP EMBRYOS FOLLOWING SINGLE-CELL BIOPSY ON DAY 4

K. Hartwich, B. Peachey, K. Cockrem, A. Marsh and A. Pugh

pp. 226-227

153 EFFECTS ON SEX RATIO AND PREGNANCY RATES OF IN VIVO-DERIVED BOVINE EMBRYOS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION SEXING METHOD

A. Ideta, S. Iwasa, T. Takedomi, M. Urakawa, M. Konishi and Y. Aoyagi

pp. 227-227

154 EFFECTS OF BLASTOMERE BIOPSY AND OXYGEN CONCENTRATION DURING CULTURE ON THE DEVELOPMENT AND INTERFERON-TAU SECRETION OF BOVINE EMBRYOS

K.M. Johnson, X. Alvarez and H.M. Kubisch

pp. 227-228

155 DEVELOPMENT OF BOVINE AGGREGATE EMBRYOS CONSTRUCTED FROM NUCLEAR TRANSFER EMBRYOS AND ELECTROFUSED IVF-DERIVED EMBRYOS

A.M. Landry, M. Murakami, R.S. Denniston, J.L. Williams, Y. Echelard and R.A. Godke

pp. 228-228

156 ENHANCED BOVINE EMBRYONIC DEVELOPMENT AFTER MICROFLUIDIC CUMULUS CELL REMOVAL POST-FERTILIZATION

A. Reeder, R. Monson, D. Beebe, B. Lindsey and J. Rutledge

pp. 228-229

157 HOLSTEIN-CHINESE YELLOW HYBRID RECIPIENT OOCYTES RECOVERED BY OVUM PICKUP CAN IMPROVE THE DEVELOPMENT OF CLONED BOVINE EMBRYOS

X.Y. Yang, J.G. Zhao, H.W. Li, H. Li, H.F. Liu, Y.T. Zeng and S.Z. Huang

pp. 229-229

158 PREGNANCY RATES IN RECIPIENT COWS TREATED WITH PROGESTERONE VAGINAL DEVICES AND INDUCED TO OVULATE WITH ESTRADIOL BENZOATE GIVEN AT THE TIME OF DEVICE REMOVAL OR 24 h LATER

G.A. Bo, E. Balla, L. Cutaia, L. Peres, P.S. Baruselli and R. Tribulo

pp. 229-230

159 TWIN vs. SINGLE TRANSFER OF IVP HOLSTEIN HEIFER EMBRYOS TO BEEF RECIPIENTS

A. Fischer-Brown, G. Barquero, S. Clark, C. Ferguson, F. Ireland, N. Jensen, S. Lane, B. Lindsey, P. Lopes, R. Monson, D. Northey, A. Reeder, J. Rutledge, M. Wheeler and D. Kesler

pp. 230-230

160 IN VIVO-CULTURE OF BOVINE EMBRYOS: TRANSFER OF SEMEN PRE-INCUBATED OOCYTES, ZYGOTES AND 4 TO 8 CELL STAGE EMBRYOS INTO THE BOVINE OVIDUCT

V. Havlicek, F. Wetscher, T. Huber, M. Gilles, D. Tesfaye, J. Griese, F. Rings, H. Müller, K. Schellander, G. Brem and U. Besenfelder

pp. 231-231

161 PREGNANCY RATES OBTAINED AFTER EMBRYO TRANSFER AT FIXED TIME OF IN VIVO-, IVF- AND CLONED-DERIVED EMBRYOS

J. Lagioia, M. Panarace, M. Marfil, M. Basualdo, J. Gutierrez, M. Révora and M. Medina

pp. 231-232

162 EMBRYO QUALITY AND COLOR IN HOLSTEIN FRIESIAN HEIFERS AND COWS IN RELATION TO SERUM PARAMETERS

J.L.M.R. Leroy, L. Goossens, A. Geldhof, T. Vanholder, S. De Vliegher, G. Opsomer and A. Van Soom

pp. 232-232

163 DOES LH PLUS hCG, USED TO INDUCE OVULATION IN SUPERSTIMULATED COWS, IMPROVE PREGNANCY RATES AFTER TRANSFER OF EMBRYOS THAT ARE NOT EXCELLENT?

M.F.G. Nogueira, J. Buratini Jr and C.M. Barros

pp. 232-233

164 EFFECT OF ACCUMULATION OF LIPIDS DURING IN VITRO CULTURE ON BOVINE BLASTOCYST YIELD AND FETAL DEVELOPMENT

J.A. Rooke, M. Ewen, G.J. McCallum, R.G. Watt and T.G. McEvoy

pp. 233-233

165 SYNCHRONIZATION TREATMENT WITH NEW AND REUSED CIDR-B DEVICES: ESTRUS AND PREGNANCY RATES IN AN EMBRYO TRANSFER PROGRAM

C.W. Solorzano, J.H. Mendoza, A. Villa-Godoy, C.S. Galina and S. Romo

pp. 233-234

166 EFFECT OF TREATMENT WITH hCG OR GnRH AT THE TIME OF EMBRYO TRANSFER ON PREGNANCY RATES IN COWS SYNCHRONIZED WITH PROGESTERONE VAGINAL DEVICES, ESTRADIOL BENZOATE, AND eCG

R. Tribulo, E. Balla, L. Cutaia, G.A. Bo, P.S. Baruselli and L. Peres

pp. 234-234

167 ISOLATION AND COMPARATIVE PROFILING OF HUMAN ADIPOSE-DERIVED ADULT STEM CELLS

A. Boquest, A. Shahdadfar, K. Fronsdal, J. Brinchmann and P. Collas

pp. 234-235

168 ESTABLISHMENT AND MOLECULAR CHARACTERIZATION OF PIG PARTHENOGENETIC EMBRYONIC STEM CELLS

T.A.L. Brevini, F. Cillo and F. Gandolfi

pp. 235-235

169 CULTURE OF MURINE EMBRYONIC STEM CELLS ON NWPF DISCS

G. Cetinkaya, S. Arat, H. Odaman Mercan, M.A. Onur and A. Tumer

pp. 235-235

170 ASSESSING THE POTENTIAL OF STEM CELLS TO GENERATE CHIMERIC RATS

J. Guo, S. Fida, K. Gou, C. Zhang, J. Morrison and Z. Du

pp. 236-236

171 NORMAL REPROGRAMMING OF IMPRINTING IN PARTHENOGENETIC FEMALE GERM CELLS

T. Horii, Y. Nagao, M. Kimura and I. Hatada

pp. 236-236

172 PRIMORDIAL GERM CELL DIFFERENTIATION FROM ES CELLS IN VITRO IN MOUSE

J. Kobolak, E. Deak and A. Dinnyes

pp. 236-237

173 EFFECT OF CULTURE SYSTEM FOR IVM-IVF PIG EMBRYOS ON THE ICMS ABILITY TO PRODUCE OUTGROWTHS FOR EMBRYONIC STEM CELL DERIVATION

G. Lazzari, I. Lagutina, G. Crotti, P. Turini, S. Colleoni, R. Duchi and C. Galli

pp. 237-237

174 ISOLATION, CULTURE AND POTENTIAL USE OF THE PORCINE NEURAL AND EPIDERMAL STEM CELLS

J. Motlik, P. Vodicka, J. Klima, K. Smetana, F. Liu and H. Gabius

pp. 238-238

175 ESTABLISHMENT OF PORCINE EMBRYONIC STEM CELL LINE DERIVED FROM IN VIVO BLASTOCYSTS

S.-A. Ock, B. Mohana Kumar, H.-F. Jin, L.-Y. Shi, S.-L. Lee, S.-Y. Choe and G.-J. Rho

pp. 238-238

176 IMPROVED GROWTH OF HUMAN EMBRYONIC STEM CELLS IN A REDUCED OXYGEN ATMOSPHERE

T. Peura, A. Bosman and T. Stojanov

pp. 238-239

177 GENERATION OF ES CELLS AND TRANSGENIC MICE EXPRESSING MTERT-GFP AS A MARKER OF PLURIPOTENTIAL CELLS

M.A. Ramírez, R. Fernández-González, P.N. Moreira, M. Pérez-Crespo, J. de la Fuente, B. Pintado and A. Gutiérrez-Adán

pp. 239-239

178 ESTABLISHMENT OF MOUSE PLURIPOTENT STEM CELLS GENERATED FROM PRIMORDIAL GERM CELLS

S.W. Shim, S.J. Song, H.S. Shim, S.J. Uhm, B.Y. Lee, S.B. Kim, H. Song, S-.G. Cho, H.M. Chung, K.S. Chung and H.T. Lee

pp. 239-240

179 ISOLATION AND CULTURE OF EMBRYONIC GERM-LIKE CELLS FROM PORCINE MESONEPHROS

H.-Y. Son, M.-G. Kim, J.-I. Yun, J.-E. Kim, H.-S. Kim, S.-K. Kang, B.-C. Lee, W.-S. Hwang and C.-K. Lee

pp. 240-240

180 EFFICIENT GENE SILENCING USING siRNA IN MOUSE AND MONKEY ES CELLS AND DIFFERENTIATION

T. Takada, K. Nemoto, A. Yamashita, M. Katoh, Y. kondo and R. Torii

pp. 240-241

181 Oct-4: A POTENTIAL MARKER FOR PLURIPOTENCY IN CATTLE

M. Vejlsted, B. Avery, J. Gjoerret, M. Schmidt, T. Greve and P. Maddox-Hyttel

pp. 241-241

182 DISINFECTION OF DRY (VAPOR) SHIPPERS ('DEWARS') FROM MICROBIAL CONTAMINATION ASSOCIATED WITH CRYOPRESERVED GERMPLASM

A. Bielanski

pp. 241-242

183 EFFECT OF THE ACIDIC ORGANIC BUFFER MES ON BOVINE IN VITRO EMBRYO PRODUCTION

K. de Haas, I. Luther and D. Gerber

pp. 242-242

184 THE EFFECT OF A NOVEL SEMEN DISINFECTION TREATMENT ON THE VIABILITY AND FERTILIZING CAPACITY IN VIVO OF BOVINE SPERMATOZOA

M. de la Rey, K.A. Morfeld, R. Treadwell and N.M. Loskutoff

pp. 242-242

185 A NOVEL AND EFFECTIVE PROCEDURE FOR REMOVING HIV-1 AND HEPATITIS B AND C VIRUSES FROM SPIKED HUMAN SEMEN

N.M. Loskutoff, C. Huyser, R. Singh, K.A. Morfeld, D.L. Walker, A.R. Thornhill, M. Smith, L. Morris and L. Webber

pp. 243-243

186 A NOVEL METHOD FOR ELIMINATING PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS FROM BOAR SEMEN AND ITS EFFECTS ON EMBRYO DEVELOPMENT

K.A. Morfeld, B. White, G. Mills, R. Krisher, M.A. Mellencamp and N.M. Loskutoff

pp. 243-243

187 ANALYZING DISEASE TRANSMISSION RISKS FROM ABATTOIR-DERIVED IN VITRO-PRODUCED BOVINE EMBRYOS

G. Perry

pp. 244-244

188 DIRECT-THAW TRANS-CERVICAL TRANSFER OF RED DEER FROZEN IN VITRO BLASTOCYSTS CAN RESULT IN PREGNANCIES

S.E. Beaumont, M.C. Berg, K. Strongman, D.P. Saywell and D.K. Berg

pp. 244-244

189 PRODUCTIVE AND REPRODUCTIVE PROFILE OF HOLSTEINS KEPT IN BALOCHISTAN PROVINCE, PAKISTAN

M. Bilal, M.Y. Lodhi, S. Chawanakul and M.A. Kakar

pp. 245-245

190 A PROCEDURE TO OBTAIN FIBROBLASTS FROM WILD ANIMALS

J.N. Caamaño, M. Hermsen, J. Marcos, A. Meana, C. Alonso, F. Goyache, M. Prieto, A. Espí, L.J. Royo, C. Diez, G. Pajares, D. Villanúa, S. Borragán, R.S. Prather and E. Gómez

pp. 245-245

191 TIMING OF OVULATION IN THE GONADOTROPHIN-STIMULATED SOUTHERN HAIRY-NOSED WOMBAT, LASIORHINUS LATIFRONS

G.V. Druery, M.D. Rival, D.A. Taggart, G.A. Shimmin, A.B. Horsup, P.D. Temple-Smith, D.B.B.P. Paris and M.C.J. Paris

pp. 246-246

192 PROBLEMS USING JC-1 TO ASSESS MITOCHONDRIAL STATUS IN BROWN BEAR (URSUS ARCTOS) SEMEN

V. García-Macías, F. Martínez-Pastor, F. Martínez, N. González, M. Álvarez, E. Anel, P. Paz, S. Borragan, M. Celada and L. Anel

pp. 246-246

193 TESTIS TISSUE XENOGRAFTING TO PRESERVE GERM CELLS FROM A CLONED BANTENG CALF

A. Honaramooz, W. Zeng, R. Rathi, J. Koster, O. Ryder and I. Dobrinski

pp. 247-247

194 BIRTH OF KITS AFTER STORAGE IN CULTURE AND TRANSFER OF IN VIVO EMBRYOS IN THE FARMED EUROPEAN POLECAT MUSTELA PUTORIUS)

H. Lindeberg, K. Kananen-Anttila, M. Eronen, E. Reinikainen, A. Helin and M. Halmekytö

pp. 247-247

195 CHANGES OF BLESBOK AND BLUE WILDEBEEST EPIDIDYMAL SPERM AFTER INCUBATION AT 37°C

F. Martinez-Pastor, F. Olivier, T. Spies, L. Anel and P. Bartels

pp. 248-248

196 IN VITRO DEVELOPMENT OF RECONSTRUCTED WATER BUFFALO (BUBALUS BUBALIS) OOCYTES AFTER FETAL SKIN FIBROBLAST CELL NUCLEAR TRANSFER

C.R. Meena and S.K. Das

pp. 248-248

197 HYSTRICOMORPH SUBPLACENTA: THE COMPARATIVE ASPECTS

M.A. Miglino, M. Bonatelli, M.C. Lima, M.F. Oliveira, R.F. Rodrigues, R.G. Carvalho, C.E. Ambrósio, M.R.F. Machado and A.M. Carter

pp. 249-249

198 DISTRIBUTION OF SPERMATOZOA AND COPULATORY PLUG IN RELATION TO THE TIME OF MATING AND OVULATION IN THE FEMALE TAMMAR WALLABY (MACROPUS EUGENII)

D.B.B.P. Paris, D.A. Taggart, M.C.J. Paris, P.D. Temple-Smith and M.B. Renfree

pp. 249-249

199 BASIC CHARACTERISTICS AND CRYOBANKING OF BARBARY SHEEP (AMMOTRAGUS LERVIA) SEMEN

S.S. Pérez-Garnelo, C. Borque, N. Madrid-Bury, M. Delclaux, C. Talavera, E. Martínez, A.T. Palasz and J. De La Fuente

pp. 249-250

200 IMMUNOHISTOCHEMICAL LOCALIZATION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND ITS RECEPTORS, Flt-1 AND KDR, IN THE COLLARED PECCARY (TAYASSU TAJACU) PLACENTA

T.C. Santos, P.C. Papa, V. Dantzer and M.A. Miglino

pp. 249-250

201 EFFECT OF HEPARIN AND CALCIUM IONOPHORE ON ACROSOME REACTION IN EPIDIDYMAL SPERMATOZOA OF DROMEDARY CAMEL (CAMELUS DROMEDARIUS)

N.A. Wani and M.A. Nowshari

pp. 250-251

202 GENETIC INFLUENCE ON FOLLICULAR DEVELOPMENT IN CATTLE

S. Chaubal, S. Bartolotta, M. Belski, G. Cimmino, H. Claus, C. Megyola, M. Orefice, B. Henderson, X. Yang and X.C. Tian

pp. 251-251

203 ASSESSMENT OF OVARIAN RESERVE. IS THERE A ROLE FOR OVARIAN BIOPSY?

R. De Roover and C. Hanzen

pp. 252-252

204 ASSOCIATION BETWEEN SOME ULTRASONIC CHARACTERISTICS OF CORPUS LUTEUM AND PROGESTERONE VALUES IN CASPIAN MINIATURE MARES

F. Gharagozloo and A. Shirazi

pp. 252-253

205 DEVELOPMENT OF OOCYTES FROM COWS TREATED WITH RETINOL IS COMPROMISED PRIOR TO IMPLANTATION

C. Hidalgo, C. Díez, A. Rodríguez, N. Facal, J.M. Prendes, C. Alonso-Montes, S. Ikeda, E. Morán and E. Gómez

pp. 253-253

206 FOLLICULAR GROWTH SUBSEQUENT TO FOLLICULAR ASPIRATION IN THE MARE

L.N. Holbech, K.D. Frederiksen, H.G. Pedersen, T. Greve and I.B. Bøgh

pp. 253-253

207 OOCYTE SURVIVAL AND FOLLICULAR DEVELOPMENT IN Fas-KNOCKOUT AND KIT-DEFICIENT DOUBLE MUTANT MICE

M. Moniruzzaman, K.O. Sakamaki, Y. Akazawa and T. Miyano

pp. 254-254

208 FOLLICULAR DYNAMICS, ESTRUS, AND OVULATION IN COWS SYNCHRONIZED TO OVULATE FIRST OR SECOND WAVE DOMINANT FOLLICLES USING SHORT- OR LONG-TERM MELENGESTROL ACETATE- OR EAZI-BREED CIDR INSERT-BASED PROTOCOLS

D.J. Schafer, J.F. Bader, D.C. Busch, F.N. Kojima, M.R. Ellersieck, M.C. Lucy, M.F. Smith and D.J. Patterson

pp. 254-254

209 NORGESTOMET IMPLANTS REDUCE LH RELEASE PATTERN IN ZEBU COWS UNDERGOING REPEATED OOCYTE PICK-UP

J.H.M. Viana, L.S.A. Camargo, A.M. Ferreira, W.F. Sa, C.A.C. Fernandes and A.P. Marques Junior

pp. 255-255

210 COMPARISON OF REAL-TIME PCR AND END-POINT PCR FOR ANALYSIS OF GENE EXPRESSION IN PREIMPLANTATION EMBRYOS

A. Baji Gal, J.W. Carnwath, A. Dinnyes, D. Herrmann, C. Wrenzycki and H. Niemann

pp. 255-256

211 TEMPORAL AND SPATIAL EXPRESSION PROFILE OF THE UTERINE MILK PROTEIN — A MEMBER OF THE SERINE PROTEASE INHIBITOR SUPERFAMILY — IN THE BOVINE ENDOMETRIUM

S. Bauersachs, S.E. Ulbrich, K. Gross, S. Schmidt, H. Wenigerkind, H.H.D. Meyer, H. Blum and E. Wolf

pp. 256-256

212 HETEROGENEITY OF RIBOSOMAL RNA GENE ACTIVATION AMONG CELLS OF IN VITRO-PRODUCED PORCINE EMBRYOS

B. Bjerregaard, F. Strejcek, Z. Rasmussen, J. Laurincik, H. Niemann, P. Maddox-Hyttel and P.D. Thomsen

pp. 256-257

213 SAGE ANALYSIS OF TRANSITIONS IN THE PORCINE CONCEPTUS TRANSCRIPTOME DURING TROPHECTODERM ELONGATION

L. Blomberg, T. Sonstegard, C. Van Tassell and K. Zuelke

pp. 257-257

214 DIFFERENT GENE EXPRESSION OF INDIVIDUAL BLASTOMERES IN EARLY MOUSE EMBRYO DETECTED BY REAL TIME PCR

Sz. Bodo, A. Baji Gal, D. Boonkusol, B. Gorhony, E. Balogh and A. Dinnyes

pp. 258-258

215 COMPARISON OF STRONG ENDOTHELIAL CELL-SPECIFIC PROMOTERS FOR EXPRESSION OF HUMAN COMPLEMENT REGULATORY PROTEINS IN PORCINE XENOGRAFT ENDOTHELIAL CELLS

T.W. Choi, J.H. Kim, H.S. Choi, S.J. Uhm, C.S. Park, H.T. Lee and S.G. Cho

pp. 258-258

216 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN BOVINE EMBRYOS CULTURED IN VIVO OR IN VITRO

D. Corcoran, T. Fair, D. Rizos, G.W. Smith, P.M. Coussens, O.V. Patel, J.J. Ireland, M.P. Boland, A.C.O. Evans and P. Lonergan

pp. 259-259

217 PROMOTER-SPECIFIC EXPRESSION OF THE IMPRINTED Igf2 GENE IN CATTLE

C. Curchoe, S. Zhang, Y. Bin, L. Yang and X.C. Tian

pp. 259-259

218 SELECTIVE REDUCTION OF p66^shc mRNA IN BOVINE OOCYTES/EMBRYOS BY RNA INTERFERENCE

L.A. Favetta, G.F. Mastromonaco, E.J. St. John, W.A. King and D.H. Betts

pp. 260-260

219 METHYLATION STATUS OF A DIFFERENTIALLY METHYLATED REGION (DMR) WITHIN THE BOVINE Igf2 GENE IN PREIMPLANTATION EMBRYOS

C. Gebert, C. Wrenzycki, D. Herrmann, R. Reinhardt, D. Gröger, A. Lucas-Hahn, J.W. Carnwath and H. Niemann

pp. 260-260

220 AN EXPRESSION PROFILE OF GENES CRUCIAL FOR PLACENTAL DEVELOPMENT IN SINGLE IN VIVO, IN VITRO AND CLONED BOVINE BLASTOCYSTS

V. Hall, N. Ruddock, R. Tecirlioglu, M. Cooney and A. French

pp. 261-261

221 TYPICAL HIF1-REGULATED GENES ARE UNALTERED BY OXYGEN IN BOVINE BLASTOCYSTS

A. Harvey, K. Kind and J. Thompson

pp. 261-261

222 METHYLATION OF THE 52-UPSTREAM REGION OF THE H19 GENE IN MOUSE SOMATIC CELL, GAMETES, WILD TYPE AND ANDROGENETIC ES CELLS

H. Kato, H. Murakami, M. Kawasumi, T. Kunieda, M. Okuno, M. Kishimoto, M. Soma, D. Iwai, M. Anzai, T. Mitani, K. Matsumoto, K. Saeki, Y. Hosoi and A. Iritani

pp. 261-262

223 ABERRENT TIMP-2 GENE EXPRESSION IN CLONED BOVINE NEONATAL PLACENTA IS DUE TO ABNORMAL EPIGENETIC MODIFICATIONS

H.R. Kim, J.K. Kang, J.T. Yoon, J.K. Jung, H.H. Seong, C.S. Park, T. Wakayama and D.I. Jin

pp. 262-262

224 TETRACYCLINE-INDUCIBLE GENE EXPRESSION WITH RETROVIRUS VECTOR

B.C. Koo, M.S. Kwon, I. Jeon, W. Chang and T. Kim

pp. 262-263

225 RECOMBINANT HUMAN ERYTHROPOIETIN PRODUCED IN THE MILK OF TRANSGENIC MICE: FUNCTIONAL CHARACTERIZATION AND PHARMACEUTICAL APPROACH

D.N. Kwon, J.Y. Park, S.Y. Lee, S.J. Kang and J.H. Kim

pp. 263-263

226 GREATER DYSREGULATION OF GENE EXPRESSION IN PRE-IMPLANTATION CLONED OVINE COMPARED WITH CLONED BOVINE CONCEPTUSES

N. Li, T. Xiang, A. Ledgard, J. Peterson, D. Wells and R. Lee

pp. 263-264

227 CONSTRUCTION OF STAGE-SPECIFIC cDNA MICROARRAY, AND ANALYSIS OF IN VITRO PRODUCED PRE-IMPLANATION STAGE BOVINE EMBRYOS FOR DEVELOPMENTAL COMPETENCE

S. Mamo, C.A. Sargent, N.A. Affara, K. Wimmers, S. Ponsuksili, M. Gilles and K. Schellander

pp. 264-264

228 EXPRESSION OF zag1 IN MOUSE PRE-IMPLANTATION EMBRYOS

K. Matsumoto, A. Uenoyama, T. Matsuoka, K. Saeki, Y. Hosoi and A. Iritani

pp. 264-264

229 EFFECT OF CULTURE CONDITIONS ON AQUAPORIN mRNA ABUNDANCE IN MOUSE BLASTOCYSTS

H. Offenberg and P.D. Thomsen

pp. 265-265

230 EFFECT OF IN VITRO CULTURE TREATMENT AND DONOR EWE DIET ON IGF2R EXPRESSION IN FETAL TISSUES

K.A. Powell, K. Mackie, T.G. McEvoy, K.D. Sinclair, J.J. Robinson, C.J. Ashworth, L.E. Young, I. Wilmut and J.A. Rooke

pp. 265-265

231 TEMPORAL AND SPATIAL GENE EXPRESSION ANALYSIS OF THE BOVINE OVIDUCT EPITHELIUM

S. Rehfeld, S. Bauersachs, H. Blum, S. Mallok, H. Wenigerkind and E. Wolf

pp. 266-266

232 SPECIES-RELATED DIFFERENCES IN BLASTOCYST QUALITY ARE ASSOCIATED WITH DIFFERENCES IN RELATIVE mRNA EXPRESSION

D. Rizos, A. Gutierrez-Adan, P. Moreira, C. O'Meara, T. Fair, A.C.O. Evans, M.P. Boland and P. Lonergan

pp. 266-266

233 EXPRESSION AND LOCALIZATION OF DNMT1 DURING EARLY BOVINE DEVELOPMENT

D.F. Russell and D.H. Betts

pp. 267-267

234 REGION SPECIFIC ABUNDANCE OF INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) IN THE BOVINE OVIDUCT DURING THE ESTROUS CYCLE

S.E. Ulbrich, S. Rehfeld, R. Rottmayer, S. Hiendleder, E. Wolf, H.H.D. Meyer and R. Einspanier

pp. 267-267

235 THE INVESTIGATION OF mRNA EXPRESSION OF SEVERAL CHROMATIN REMODELLING GENES DURING BOVINE PREIMPLANTATION DEVELOPMENT

K. Wilson, M. Cooney, A. French, M. Holland, P. Verma and N. Ruddock

pp. 268-268

236 SPATIAL GENE EXPRESSION PATTERNS OF Dnmt1, Dnmt3a, AND Hdac2 IN PREIMPLANTATION EMBRYOS

C. Wrenzycki, D. Herrmann, A. Lucas-Hahn, K. Korsawe, E. Lemme and H. Niemann

pp. 268-268

237 INCREASE OF BLASTOCYST DEVELOPMENTAL RATE IN VITRO BY SELECTION OF DEVELOPMENTALLY COMPETENT COW OOCYTES BEFORE IVM USING A STAINING TEST

H. Alm, H. Torner, B. Loehrke, T. Viergutz, I. Ghoneim and W. Kanitz

pp. 269-269

238 ASSESSMENT OF SPERM MOTILITY, VIABILITY, AND FERTILIZATION RATE OF TWO GROUPS OF BULLS SHOWING A DIFFERENT ABILITY TO PROMOTE EMBRYO DEVELOPMENT IN VITRO

M. Alomar, J. Mahieu, B. Verhaeghe and I. Donnay

pp. 269-269

239 CAPACITATION OF BUFFALO SPERMATOZOA IN VITRO

L. Boccia, A. De Rosa, L. Attanasio, R. Di Palo, L. Zicarelli and B. Gasparrini

pp. 270-270

240 GESTATION LENGTH AND BIRTH WEIGHT OF IN VITRO PRODUCED EMBRYOS FROM ZEBU DAIRY CATTLE

L.S.A. Camargo, J.H.M. Viana, A.A. Ramos, W.F. de Sa, A.M. Ferreira, J.F. Fonseca and V.R. Vale Filho

pp. 270-270

241 EFFECT OF MEIOTIC ARREST BY ROSCOVITINE AND SUBSEQUENT IVM TIME ON DEVELOPMENTAL COMPETENCE OF IMMATURE BOVINE OOCYTES

L. Campos-Chillon, T. Suh, E. Carnevale and G. Seidel

pp. 271-271

242 DEVELOPMENT OF BAC FISH PROBES FOR GENETIC ANALYSIS OF NON-HUMAN PRIMATE GAMETES AND EMBRYONIC STEM CELLS

L. Froenicke, S.M. Nichols, H.M. Kubisch, L.A. Lyons, B.D. Bavister and C.A. Brenner

pp. 271-272

243 PREGNANCY RATES IN THE FIELD AFTER THE TRANSFER OF BOVINE IVP EMBRYOS VITRIFIED BY THE CRYOLOGIC VITRIFICATION METHOD

R. Fry, C. Earl, K. Fry and W. Lindemans

pp. 272-272

244 EFFECT OF β-MERCAPTOETHANOL IN A TRANSIENT-IVF SYSTEM ON SPERM PENETRATION INTO PORCINE IVM OOCYTES

H. Funahashi

pp. 272-273

245 OOCYTE RECOVERY BY OVUM PICK UP AND EMBRYO PRODUCTION IN MURRAH AND NILI-RAVI BUFFALOES (BUBALUS BUBALIS) IMPORTED IN CHINA

Y. Huang, X. Zhang, B. Gasparrini and G.A. Presicce

pp. 273-273

246 FOLLICULAR DEVELOPMENT AND OOCYTE QUALITY AFTER OVUM PICKUP IN DONOR COWS

K. Imai, M. Tagawa, S. Matoba, M. Narita and N. Saito

pp. 273-274

247 USING THE ACIDIC ORGANIC BUFFER MES TO CLEAN IVP BOVINE EMBRYOS FROM FOOT AND MOUTH DISEASE VIRUS

F. Jooste, K. de Haas, K. Boshoff, W. Vosloo and D. Gerber

pp. 274-274

248 EMBRYO DEVELOPMENT IN MICRODROPS AND MICROCHANNELS: COMPARISON OF NCSU23 SEQUENTIAL AND NON-SEQUENTIAL CULTURE MEDIUM

A.S. Lima, C.E. Ferguson and M.B. Wheeler

pp. 274-275

249 EFFECT OF REFREEZING BULL SEMEN ON IVF SUCCESS RATE

P. McCue, J. Kelly, S. Ashworth, D. Kleemann and S. Walker

pp. 275-275

250 DEHYDRATED COCONUT WATER FOR IN VITRO SPERM CAPACITATION IN SWINE

A.B. Nascimento, V.P. Oliveira, M.G. Marques, R. Toniolli, R.P.C. Gerger, A.R.S. Coutinho, W.B. Feitosa and J.A. Visintin

pp. 275-275

251 CAMELID EMBRYO DEVELOPMENT IN VITRO: EFFECT OF PROTEIN SUPPLEMENT IN MATURATION MEDIUM AND SUBSEQUENT CULTURE IN TWO DIFFERENT MEDIA ON FERTILIZATION AND DEVELOPMENT

M.A. Nowshari and N.A. Wani

pp. 276-276

252 COMPARISON OF TWO SPERM SEPARATION PRODUCTS FOR USE IN BOVINE IVF

J. Pryor, S. Romo, D.D. Varner, K. Hinrichs and C.R. Looney

pp. 276-276

253 COMPARISON OF THREE DIFFERENT IVF PROTOCOLS FOR BOVINE OOCYTES

S. Romo, J. Pryor, D.D. Varner, K. Hinrichs and C.R. Looney

pp. 277-277

254 EFFECT OF FSH TREATMENT ON NUMBER OF BOVINE OOCYTES RETRIEVED BY NON-ULTRASOUND GUIDED TRANSVAGINAL OVUM PICK-UP AND THEIR DEVELOPMENTAL COMPETENCE

A. Sathanawongs, S. Rojanasthien, A. Oranratnachai and J. Sumretprasong

pp. 277-277

255 PRONUCLEUS FORMATION IN RAT ZONA-FREE OOCYTES CO-CULTURED WITH HOMOLOGOUS POST-THAW SPERMATOZOA

Y. Seita, Y. Okuda, A. Takizawa, N. Hirahara, M. Koichi, Y. Obinata, M. Shino, T. Inomata and N. Kashiwazaki

pp. 277-278

256 COMMERCIAL-SCALE IN VITRO PRODUCTION AND TRANSFER OF JAPANESE BLACK EMBRYOS

H. Seizo, M. Motoharu, T. Hideki, H. Atsushi and K. Takeshi

pp. 278-278

257 EFFECTS OF GLUTAMINE AND HYPOTAURINE ON OXIDATIVE STRESS OF PORCINE EMBRYOS CULTURED IN VITRO

C. Suzuki and K. Yoshioka

pp. 278-279

258 APOPTOSIS IN FAST- AND SLOW-CLEAVING BOVINE EMBRYOS IN VITRO

L. Vandaele, B. Mateusen, D. Maes and A. Van Soom

pp. 258-258

259 INVOLVEMENT OF Ca²⁺/CALMODULIN-DEPENDENT PROTEIN KINASE II IN THE SPONTANEOUS ACTIVATION IN RAT OOCYTES

J.G. Yoo and L.C. Smith

pp. 279-280

260 REPLACEMENT OF PVA WITH FETAL BOVINE SERUM IMPROVES FORMATION AND HATCHING OF PORCINE BLASTOCYSTS PRODUCED IN VITRO

K. Yoshioka, C. Suzuki and H. Rodriguez-Martinez

pp. 280-280

261 FERTILITY CONTROL BY GnRH ANALOGUES IN DOGS

G. Aiudi, M. Albrizio, G. De Vico, A. Scirpo, S. Cristarella and M. Cinone

pp. 280-281

262 SPERM CHROMATIN STRUCTURE, OXIDATIVE STRESS AND BASIC SEMEN PARAMETERS OF MEN FROM SUBFERTILE COUPLES

M. Bochenek, P. Gogol and J. Janeczko

pp. 281-281

263 IDENTIFICATION OF A NOVEL MOPT GENE IN HUMAN AND MOUSE ADULT TESTIS

Y.-J. Choi, S.-J. Kang and J.-H. Kim

pp. 281-282

264 PORCINE SPERM-HEAD RECEPTOR INTERACTION WITH PROTEINS PERIPHERALLY BOUND TO THE OVIDUCTAL LUMEN

R.M.A. Elliott, N. Satake, P.F. Watson, A.R. Fazeli and W.V. Holt

pp. 282-282

265 SPERM DNA FRAGMENTATION AND PREGNANCY OUTCOME

D. Evenson

pp. 282-282

266 ANTIOXIDANT CAPACITY OF BOAR SEMINAL PLASMA

M. Hernandez, A. Cano, M.B. Arnao, X. Lucas, J.M. Vazquez, E.A. Martinez and J. Roca

pp. 283-283

267 TESTIS TISSUE XENOGRAFTING AS A BIOASSAY FOR GERM CELL DEVELOPMENTAL POTENTIAL IN EQUINE CRYPTORCHID TESTES

R. Rathi, A. Honaramooz, W. Zeng, R. Turner and I. Dobrinski

pp. 283-283

268 GERM CELL DEVELOPMENT IN EQUINE TESTIS TISSUE XENOGRAFTED INTO MICE

R. Turner, R. Rathi, A. Honaramooz, W. Zeng and I. Dobrinski

pp. 283-284

269 INACTIVATION OF MATURATION PROMOTING FACTOR AND MITOGEN-ACTIVATED PROTEIN KINASE IN PORCINE OOCYTES BY A SINGLE ELECTRICAL PULSE

D.-B. Koo, J.-I. Chae, J.-S. Kim, G. Wee, B.-S. Song, K.-K. Lee and Y.-M. Han

pp. 284-284

270 COMBINED ELECTRICAL AND CHEMICAL ACTIVATION OF ZONA-FREE PORCINE OOCYTES

P.M. Kragh, N.R. Mtango, T.J. Corydon, L. Bolund, H. Callesen and G. Vajta

pp. 284-285

271 ASSESSMENT OF NUCLEAR STATUS OF ACTIVATED BOVINE OOCYTES MATURED IN DIFFERENT MATURATION CONDITIONS IN VITRO

J.I. Park and Y. Jang

pp. 285-285

272 OOCYTE SPONTANEOUS ACTIVATION IN DIFFERENT RAT STRAINS

P. Ross, A. Yabuuchi and J. Cibelli

pp. 285-286

273 OPTIMIZATION OF PROTOCOLS FOR ACTIVATION OF GOAT OOCYTES WITH IONOMYCIN IN COMBINATION WITH 6-DIMETHYLAMINOPURINE

J.-H. Tan, G.-C. Lan, Z.-L. Chang, D. Han, Z.-B. Han, N. Liu, S.-F. Ma and M.-J. Luo

pp. 286-286

274 NUCLEAR MATURATION KINETICS AND IN VITRO EMBRYO DEVELOPMENT OF BOVINE OOCYTES TREATED WITH BUTYROLACTONE I COMBINED OR NOT WITH ROSCOVITINE

P.R. Adona, M.D. Quetglas, P.R.L. Pires and C.L.V. Leal

pp. 287-287

275 THE EFFECTS OF MATURATION CULTURE PERIOD ON THE SEX RATIO OF BOVINE IVF EMBRYOS

B. Agung, P. Wongsrikeao, M. Murakami, H. Watari and T. Otoi

pp. 287-288

276 THE EFFECT OF CUMULUS CELLS DURING MATURATION ON THE RISE IN THE CONCENTRATION OF INTRACELLULAR Ca²⁺ ([Ca²⁺]i) OF PORCINE OOCYTES INDUCED BY INOSITOL 1,4,5-TRISPHOSPHATE

T. Amano, T. Mori, K. Matsumoto, T. Watanabe and A. Iritani

pp. 288-288

277 NUCLEAR STAGE AND p34cdc2 EXPRESSION IN DIFFERENT SIZES OF PREPUBERTAL GOAT OOCYTES

B. Anguita, A.R. Jimenez-Macedo, D. Izquierdo and M.T. Paramio

pp. 288-289

278 MEIOTIC RESUMPTION IN VITRO OF CANINE OOCYTES: COMPARATIVE METHODS

L. Bogliolo, F. Ariu, M.T. Zedda, S. Pau and S. Ledda

pp. 289-289

279 PLASMA MEMBRANE ELECTRICAL PROPERTIES AND INTRACELLULAR CALCIUM STORES IN IMMATURE AND IN VITRO-MATURED ADULT AND JUVENILE SHEEP OOCYTES

R. Boni, N. Cocchia, F. Silvestre, G. Tortora, R. Lorizio and E. Tosti

pp. 290-290

280 EFFECTS OF β-ENDORPHIN AND NALOXONE ON INTRACELLULAR CALCIUM LEVELS IN CUMULUS CELLS OF EQUINE OOCYTES

T. De Santis, M.E. Dell'Aquila, F. Maritato, V. Casavola and P. Minoia

pp. 290-290

281 MITOGEN-ACTIVATED PROTEIN KINASE IN PORCINE CUMULUS CELLS

S. Ebeling, C. Boesebeck and B. Meinecke

pp. 291-291

282 EFFECT OF THE MATURATION TIME ON THE OUTPUT OF THE INTRACYTOPLASMIC SPERM INJECTION (ICSI) OF PIG OOCYTES PRECULTURED WITH ROSCOVITINE

E. García-Roselló, C. Matás, F. García-Vázquez, S. Cánovas and P. Coy

pp. 291-291

283 DEVELOPMENT OF CAPRINE EMBRYOS PRODUCED BY ICSI AND IVF AND CULTURED IN DIFFERENT MEDIA

A.R. Jimenez-Macedo, B. Anguita, D. Izquierdo and M.T. Paramio

pp. 291-292

284 KINETICS OF OOCYTE MATURATION AND SUBSEQUENT DEVELOPMENT OF PARTHENOGENETIC PORCINE EMBRYOS AFTER MEIOTIC INHIBITION WITH ROSCOVITINE

D.H. Kim, S.W. Kim, G.S. Im, B.C. Yang, D.R. Lee, H.S. Park, I.S. Hwang, J. S Seo, B.S. Yang and W.K. Chang

pp. 292-292

285 SUPPRESSION OF MPF AND MAPK ACTIVITIES BY DIBUTYRYL CAMP DURING FIRST MEIOTIC MATURATION IMPROVES SUBSEQUENT DEVELOPMENT OF PORCINE OOCYTES

J.-S. Kim, D.-B.n Koo, J.-I. Chae, Y.-K. Choo, K.-K. Lee and Y.-M. Han

pp. 293-293

286 THE EFFECT OF IN VITRO MATURATION MEDIUM ON CRYOSURVIVAL, CELL NUMBERS AND APOPTOTIC INDEXES OF BOVINE EMBRYOS

K. Korhonen, J. Matomäki, E. Ketoja, K. Kananen-Anttila, M. Halmekytö, M. Räty and J. Peippo

pp. 293-293

287 INHIBITION OF THE PENTOSE PHOSPHATE PATHWAY RESULTS IN MEIOTIC ARREST IN PORCINE OOCYTES THAT CAN BE OVERCOME BY THE ADDITION OF PATHWAY COFACTORS AND END PRODUCTS

R. Krisher

pp. 293-294

288 RELATIONSHIP BETWEEN CHROMATIN ORGANIZATION AND OOCYTE–CUMULUS CELL COMMUNICATION IN GERMINAL VESICLE STAGE BOVINE OOCYTES

V. Lodde, C. Galbusera, S. Modina, M.S. Beretta, A. Lauria and A.M. Luciano

pp. 294-295

289 EFFECTS OF THE STORAGE OF BOVINE OVARIES ON THE NUCLEAR MATURATION AND DEVELOPMENT OF IN VITRO PRODUCED EMBRYOS

M. Narita, S. Goda, Y. Inaba, K. Imai, S. Matoba, M. Tagawa, T. Nagai, N. Saito, M. Yonai and O. Dochi

pp. 295-295

290 MODULATION OF ARYLHYDROCARBON RECEPTOR ACTIVITY DURING IN VITRO MATURATION OF BOVINE OOCYTES

D. Nestler, M. Risch, B. Fischer and P. Pocar

pp. 295-296

291 EFFECT OF DIFFERENT TRANSPORT TEMPERATURES (+4°C, +32°C) ON IN VITRO MATURATION OF OOCYTES COLLECTED FROM CATTLE AND SHEEP OVARIES

O.B. Ozdas, M. Tas, U. Cirit, M. Evecen, K. Demir, S. Bacinoglu, K. Ak and I.K. Ileri

pp. 296-296

292 MATURATION IN A STRAW IS EFFECTIVE ON THE DEVELOPMENT OF BOVINE OOCYTES IN VITRO

Y.M. Park, S.S. Kim, J.H. Lee, Y.S. Park and H.D. Park

pp. 296-297

293 TWO-STEP MATURATION OF BOVINE OOCYTES WITHOUT CDK INHIBITORS: AN ALTERNATIVE TO AFFECT THEIR SUBSEQUENT DEVELOPMENTAL COMPETENCE

A. Pavlok, G. Lapathitis, S. Cech, M. Kubelka, M. Lopatarova, L. Holy, J. Klima, J. Motlik and V. Havlicek

pp. 297-297

294 EFFECT OF CYSTEAMINE ADMINISTRATION DURING EQUINE OOCYTE MATURATION ON GLUTATHIONE CONTENT, NUCLEAR MATURATION, AND DEVELOPMENTAL CAPABILITY AFTER INTRACYTOPLASMIC SPERM INJECTION

F. Perazzoli, C. Galbusera, S. Modina, G. Goudet, N. Gerard and A.M. Luciano

pp. 297-298

295 COMPARISON OF TWO METHODS TO AVOID MOVEMENT OF BOVINE OOCYTES DURING IN VITRO MATURATION

M.M. Petersen, B. Avery, T. Greve and I.B. Bøgh

pp. 298-298

296 LEUKEMIA INHIBITORY FACTOR INFLUENCES SHEEP OOCYTE PARTHENOGENETIC DEVELOPMENT DURING THE TRANSITION FROM GERMINAL VESICLE TO EARLY PRONUCLEAR STAGE

G. Ptak, F. Lopes and P. Loi

pp. 298-299

297 A CASE REPORT: THE OUTCOME OF IVP MAY BE RELATED TO THE BATCH OF TCM-199 IN IVM

M. Räty, E. Ketoja, K. Kananen-Anttila, K. Korhonen, J. Matomäki, M. Halmekytö and J. Peippo

pp. 299-299

298 DISRUPTION OF NUCLEAR MATURATION, APOPTOSIS AND CYTOSKELETAL CHANGES IN BOVINE OOCYTES EXPOSED TO HEAT SHOCK

Z. Roth and P.J. Hansen

pp. 299-300

299 IN VITRO DEVELOPMENT OF IMMATURE PORCINE OOCYTES FERTILIZED IN VITRO TO THE BLASTOCYST STAGE

T. Somfai, K. Kikuchi, S.Y. Medvedev, A. Onishi, M. Iwamoto, D.-I. Fuchimoto, M. Ozawa, J. Noguchi, H. Kaneko, A. Bali Papp, E. Sato and T. Nagai

pp. 300-300

300 GLUCOSAMINE SUPPLEMENTATION DURING IN VITRO MATURATION LEADS TO PERTURBED DEVELOPMENTAL CAPACITY OF BOVINE CUMULUS OOCYTE COMPLEXES

M. Sutton-McDowall, R. Gilchrist and J. Thompson

pp. 300-301

301 EFFECT OF ENERGY SUBSTRATES ON METABOLISM, NUCLEAR MATURATION, AND DEVELOPMENT OF GILT AND SOW OOCYTES DURING IN VITRO MATURATION

L. Tubman, A. Peter and R. Krisher

pp. 301-301

302 PROTEIN SUPPLEMENTATION TO IVM MEDIUM IN RELATION TO THE INCIDENCE OF APOPTOSIS IN BOVINE OOCYTES MATURED IN VITRO

E. Warzych, K. Matulewicz, A. Nogowska and D. Lechniak

pp. 302-302

303 EFFECT OF SERUM SUPPLEMENTATION AND ESTRUS CYCLE STAGE ON IN VITRO NUCLEAR MATURATION OF CANINE OOCYTES

F.Y. Heru, H.J. Oh, M.K. Kim, J. Goo, M.S. Hossein, H.J. Kim, S.K.n Kang, B.C. Lee and W.S. Hwang

pp. 302-302

304 PRODUCTION OF PORCINE EMBRYOS OF A PREDETERMINED SEX AFTER IN VITRO FERTILIZATION OF IN VITRO-MATURED OOCYTES WITH SEX-SORTED FROZEN-THAWED BOAR SPERM

R. Bathgate, K.M. Morton, B.M. Eriksson, D. Rath, B. Seig, O. Chami, T. Stojanov, W.M.C. Maxwell and G. Evans

pp. 303-303

305 FIRST REPORT OF THE USE OF SEXED SEMEN BY FLOW CYTOMETRY IN BOS INDICUS

L. Cattaneo, A. Galassi, R. Franco, A. Duarte, K. Mello, G. Jáuregui, J. Lagioia and M. Basualdo

pp. 303-303

306 EMBRYO ANALYSIS IN FIELD MOET: HIGH SUCCESS RATE AFTER OVERNIGHT CULTURE OF MICROBLADE-BIOPSIED CATTLE EMBRYOS

K. Kananen-Anttila, K. Vartia, A. Hyvönen, J. Virta, J. Peippo and M. Halmekytö

pp. 304-304

307 IN VITRO PRODUCTION OF BOVINE EMBRYOS USING FLOW-CYTOMETRICALLY SORTED SPERMATOZOA

L. Katska-Ksiazkiewicz, M. Bochenek, B. Rynska and J. Opiela

pp. 304-304

308 PREGNANCY RATE AND EMBRYO PRODUCTION AFTER INSEMINATION OF MARES WITH SEXED SORTED SEMEN

M. Panarace, M. Calvi, A. Alonso, G. Jaúregui and M. Medina

pp. 305-305

309 SEX SORTED BOAR SPERMATOZOA: TIME COURSE AND PROFILE OF THEIR IN VITRO PENETRATION ABILITY AFTER STORAGE IN THE PRESENCE OF HOMOLOGOUS SEMINAL PLASMA

I. Parrilla, J.M. Vazquez, M.A. Gil, I. Caballero, C. Almiñana, J. Roca and E.A. Martinez

pp. 305-305

310 USE OF COMPETITIVE FERTILIZATION TO EVALUATE A SIMPLER LASER FOR FLOW CYTOMETRIC SEXING OF BOVINE SPERM

J.L. Schenk, Z. Brink and T.K. Suh

pp. 306-306

311 CATTLE SEX REGULATION BY SEPARATION OF X AND Y SPERMATOZOA PRELIMINARY RESULTS OF FIELD EXPERIMENT IN POLAND

Z. Smorag, M. Bochenek and J. Pilch

pp. 306-306

312 BLASTOCYST DEVELOPMENT OF MALE AND FEMALE BOVINE EMBRYOS PRODUCED BY IVF WITH FLOW CYTOMETRICALLY-SORTED SPERM

M. Zhang, K.H. Lu and G.E. Seidel Jr

pp. 306-307

313 EFFECTS OF ETHANOL TREATMENT AFTER INTRACYTOPLASMIC SPERM INJECTION (ICSI) ON SPERM AFTER FORMATION AND THE MICROTUBULE ORGANIZATION OF BOVINE OOCYTES

N. Fujinami, Y. Hosoi, H. Kato, T. Mitani, K. Matsumoto, K. Saeki and A. Iritani

pp. 307-307

314 FULL-TERM DEVELOPMENT OF RAT OOCYTES MICROINSEMINATED WITH FREEZE-DRIED SPERMATOZOA

M. Hirabayashi, M. Kato and S. Hochi

pp. 307-308

315 AN ATTEMPT AT INDUCING DIFFERENTIATION INTO ROUND SPERMATIDS OF RAT SPERMATOGONIA BY CO-CULTURING WITH SERTOLI CELLS

Y. Iwanami, T. Kobayashi, M. Kato, M. Hirabayashi and S. Hochi

pp. 308-308

316 SUPPLEMENTAL CYSTEINE PRESENCE DURING THE DECONDENSATION OF SPERM CHROMATIN IMPROVES FERTILIZATION AND BLASTOCYST FORMATION AFTER INTRACYTOPLASMIC SPERM INJECTION IN PIGS

M. Katayama, T. Cantley, A. Rieke and B. Day

pp. 308-309

317 FACTORS AFFECTING PRODUCTION EFFICIENCY OF TRANSGENIC RATS BY ICSI-MEDIATED DNA TRANSFER

M. Kato, S. Hochi and M. Hirabayashi

pp. 309-309

318 ENHANCEMENT OF FERTILIZATION BY DIGITONIN IN ROUND SPERMATID INJECTION

S. Kishigami, E. Mizutani, S. Wakayama and T. Wakayama

pp. 309-310

319 USE OF PENTOXIFYLLINE AND HYALURONIC ACID FOR STALLION SPERM SEPARATION

G. Kutvolgyi, T. Suh, E. Carnevale and G. Seidel Jr

pp. 310-310

320 USE OF SYNTHETIC HYALURONAN OR POLYVINYLPYRROLIDONE FOR INTRACYTOPLASMIC SPERM INJECTION INTO MOUSE OOCYTES

P.N. Moreira, J. De la Fuente, A.T. Palasz and A. Gutiérrez-Adán

pp. 310-311

321 DEVELOPMENT IN VIVO AND IN VITRO OF PORCINE OOCYTES FERTILIZED BY INTRACYTOPLASMIC INJECTION OF A FREEZE–DRIED SPERM HEAD

M. Nakai, K. Kikuchi, A. Takizawa, M. Ozawa, J. Noguchi, H. Kaneko, M. Shino and N. Kashiwazaki

pp. 311-311

322 DIFFERENTIAL DEVELOPMENT OF RABBIT EMBRYOS FOLLOWING MICROINSEMINATION USING SPERM AND SPERMATIDS

N. Ogonuki, K. Inoue, H. Miki, Y. Hirose, H. Okada, N. Shimozawa, S. Takeiri, H. Nagashima, T. Sankai and A. Ogura

pp. 312-312

323 THE QUALITY OF PREOVULATORY FOLLICLES DURING FINAL MATURATION IN COWS STIMULATED WITH oFSH AND A DEFINED LH SURGE

O. Algriany, P. Vos, H. Groenendaal, A. van Gastel, B. Colenbrander and S.J. Dieleman

pp. 312-312

324 ADJUSTING SPERM CONCENTRATION USED TO INSEMINATE SUPERSTIMULATED BEEF COWS, IN ORDER TO AVOID DECLINE IN EMBRYO PRODUCTION

C.M. Barros and M.F.G. Nogueira

pp. 313-313

325 INSULIN-LIKE GROWTH FACTOR-I CONCENTRATIONS IN OVIDUCT FLUID OF SUPEROVULATED EWES DURING THE PERI-OVULAR PERIOD

M.A. Kakar, S. Maddocks, M.F. Lorimer, D.O. Kleemann and S.K. Walker

pp. 313-313

326 OPTIMIZING FREQUENCY OF FSH APPLICATION FOR SUPEROVULATORY TREATMENT IN CATTLE

G. Martens, H.P. Nohner, C. Leiding, F. Schneider, F. Becker, G. Nuernberg and W. Kanitz

pp. 313-314

327 CHARACTERIZATION OF FETAL AND ADULT FIBROBLASTS FROM NELORE BOVINE FOR NUCLEAR TRANSFER

H.V.A. Caetano, M.P. Milazzotto, M.D. Goissis, C. Yamada, M.G. Marques, M.E.O.A. Assumpcao and J.A. Visintin

pp. 314-314

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328 SOMATIC CELL ISOLATION FROM SEMEN BY PERCOLL GRADIENTS

L. Nel-Themaat, M.C. Gomez, G. Wirtu, A. Cole, K.R. Bondioli, B.L. Dresser, R.A. Godke and C.E. Pope

pp. 314-315

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329 A NOVEL SUSPENSION CULTURE SYSTEM FOR BOVINE OVIDUCT EPITHELIAL CELLS

R. Rottmayer, S.E. Ulbrich, S. Koelle, K. Prelle, H.H.D. Meyer, F. Sinowatz, E. Wolf and S. Hiendleder

pp. 315-315

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330 UTILIZING PRE-IMPLANTATION GENETIC DIAGNOSIS AND OPU-IVP-ET TO GENERATE MULTIPLE PROGENY OF PREDETERMINED GENOTYPE FROM CLONED TRANSGENIC HEIFERS

J.T. Forsyth, H.E. Troskie, P.A. Pugh, B. Brophy, D.N. Wells and G. Laible

pp. 316-316

331 MODIFIED SINGLE-STRANDED OLIGONUCLEOTIDE-RECOMBINASE COMPLEX MEDIATES GENE TARGETING IN MOUSE EMBRYOS

J.H. Kang, J.Y. Won and H. Shim

pp. 316-316

332 ENHANCEMENT OF GROWTH PERFORMANCE IN DOUBLE TRANSGENIC MICE WITH GROWTH HORMONE RECEPTOR AND IGF-1 RECEPTOR GENES

H. Ju Kim, K.i Naruse, W.S. Choi, K.S. Im, C.S. Park and D.I. Jin

pp. 317-317

333 HIGH LEVEL EXPRESSION OF BIOACTIVE RECOMBINANT HUMAN GROWTH HORMONE IN THE MILK OF A CLONED TRANSGENIC COW

D.F. Salamone, J.L. Barañao, C.B. Santos, L. Bussman, J. Artuzo, C. Carboneto, M. Paupuchado and M. Criscuolo

pp. 317-317

334 EFFECT OF CYTOPLASMIC INJECTION OF dsRNA ON TRANSIENT EXPRESSION OF THE EGFP GENE MICROINJECTED INTO RAT EMBRYOS

A. Takizawa, M. Takahashi, S. Hisamatsu, M. Nakai, M. Shino, S. Sonoki and N. Kashiwazaki

pp. 318-318

335 A PROCEDURE COMBINING ISTAT^® ANALYSIS WITH OPU TO STUDY BOVINE FOLLICULAR ENVIRONMENTS

M.C. Berg, S.E. Beaumont, A.J. Peterson and D.K. Berg

pp. 318-319

336 COMPARISON OF FETAL DEVELOPMENT AND FETAL HEARTBEAT BY ULTRASONOGRAPHY IN ARTIFICIALLY INSEMINATED AND EMBRYO-TRANSFERRED DAIRY COWS

K. Kishida, N. Shibata, K. Hata, S. Aoki, T. Nishisouzu, O. Dochi and H. Koyama

pp. 319-319

337 OVUM PICK UP IN NON-PREGNANT AND POSTPARTUM SWAMP BUFFALOES (BUBALUS BUBALIS) AFTER FSH PRETREATMENT

A. Promdireg, M. Techakumphu, W. Adulyanubap and A. Na-Chiengmai

pp. 319-320

338 SYNCHRONISATION OF OVULATION IN MERINO EWES WITH GnRH IN THE BREEDING AND NON-BREEDING SEASON

J. Reyna, P. Thomson, G. Evans and C. Maxwell

pp. 320-320

339 ULTRASOUND GUIDED OVUM PICK UP (OPU) IN PREPUBERTAL SWAMP BUFFALO USING THREE DIFFERENT VACUUM PRESSURES

M. Techakumphu, A. Promdireg, N. Phutikanit, J. Singlor, S. Thongjan, N. Onwan and A. Na-Chiengmai

pp. 320-320