CSIRO Publishing Books Journals About Us Shopping Cart You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
  Vertebrate Reproductive Science & Technology
 
Search
 
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
General Information
Review Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

 Early Alert
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

 Connect with us
facebook   youtube

Training

Publication Workshops


 

Article << Previous     |     Next >>   Contents Vol 16(6)

Developmental competence in vitro and in vivo of cryopreserved, hatched blastocysts from the dromedary camel (Camelus dromedarius)

J. A. Skidmore A C, M. Billah A, N. M. Loskutoff B

A Camel Reproduction Centre, P. O. Box 11808, Dubai, United Arab Emirates.
B Center for Conservation and Research, Henry Doorly Zoo, Omaha, NE 68107, USA.
C To whom correspondence should be addressed. email: luluskid@emirates.net.ae
 
PDF (459 KB) $25
 Export Citation
 Print
  


Abstract

The present paper describes experiments designed to investigate methods for cryopreserving embryos from dromedary camels. Because preliminary studies had shown ethanediol to be the best cryoprotectant to use for camel embryos, the current experiments were performed to determine the minimum exposure time to 1.5 m ethanediol required to achieve cryoprotection. The uteri of 30 donor camels were flushed non-surgically 8 days after mating. Embryos were recovered and 158 were assigned to one of three groups, which were exposed to 1.5 m ethanediol for either 10 min (n = 67), 5 min (n = 51) or 1 min (n = 40). Embryos were subsequently thawed and rehydrated by expelling either directly into holding medium (HM; HEPES-buffered Tyrode's medium containing sodium lactate and 3 mg mL-1 bovine serum albumin, 10% fetal calf serum, 100 IU mL-1 penicillin G, 100 μg mL-1 streptomycin and 25 μg mL-1 amphotercin B) or initially into HM containing 0.2 m sucrose for 5 or 10 min. The survival rate of all embryos immediately post-thawing, as judged by the morphological appearance of the embryos, was high (91%), but was greatly reduced after 2 h culture (59%). Ninety-two embryos were transferred to recipient camels resulting in 18 viable fetuses (1 min ethanediol exposure, n = 1/15; 5 min ethanediol exposure, n = 3/34; 10 min ethanediol exposure, n = 14/43). Of the embryos rehydrated directly in HM, six of 65 resulted in viable fetuses and those rehydrated initially in 0.2 m sucrose for 5 or 10 min resulted in nine of 47 and three of 46 fetuses respectively. From these experiments, we conclude that camel embryos can be cryopreserved using ethanediol as a cryoprotectant when the embryos are cooled slowly (to 33°C) before being plunged into liquid nitrogen for storage.

Keywords: embryos.


   
Subscriber Login
Username:
Password:  

    


 
Top  Email this page
 
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2012