CSIRO Publishing Books Journals About Us Shopping Cart You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
  Vertebrate Reproductive Science & Technology
 
Search
 
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
General Information
Review Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

 Early Alert
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

 Connect with us
facebook   youtube

Training

Publication Workshops


 

Article << Previous     |     Next >>   Contents Vol 17(4)

Embryo transfer in the dromedary camel (Camelus dromedarius) using asynchronous, meclofenamic acid-treated recipients

Julian A. Skidmore A B, M. Billah A

A The Camel Reproduction Centre, P.O. Box 11808, Dubai, United Arab Emirates.
B Corresponding author. Email: luluskid@emirates.net.ae
 
PDF (191 KB) $25
 Export Citation
 Print
  


Abstract

A total of 40 (Day 7) embryos were recovered from the uteri of 10 superovulated camels. Recipient camels (n = 30) were prepared by injection with 20 μg of the gonadotrophin-releasing hormone analogue buserelin (i.v.) to induce ovulation and then treatment with 1 g meclofenamic acid (a prostaglandin synthetase inhibitor), orally, once on Day 7 and twice daily on Days 8 and 9 after ovulation and thereafter at a dose of 1 g day-1 until 8 days after embryo transfer. Embryos were transferred into recipients on Day 8 (n = 10), Day 10 (n = 10) or Day 12 (n = 10) after ovulation and another 10 embryos were transferred into untreated recipients on Day 8 after ovulation as controls. In addition, serum samples from all recipient camels were recovered daily throughout the period of meclofenamic acid administration and for a further 7 days after treatment had ceased and were assayed for progesterone concentrations. Results showed that whereas only one of 10 of the control group of recipients (10%) was diagnosed pregnant, a total of eight of 10 Day 8 (80%), six of 10 Day 10 (60%) and seven of 10 Day 12 (70%) recipients were diagnosed pregnant by ultrasonography 12 days after the embryo had been transferred. Subsequently, however, four pregnancies were lost when the conceptus was aged between 22 and 60 days, but this is not considered above the early fetal mortality rate expected in camels after natural mating or after transferring camel embryos to untreated recipients. Serum progesterone concentrations remained elevated, above 2 ng mL-1, throughout the period of meclofenamic acid administration in all recipient camels; thereafter, concentrations remained above 2 ng mL-1 in pregnant animals, whereas in non-pregnant camels concentrations had declined to baseline values (<1 ng mL-1) within 3 days of the end of the treatment period. In conclusion, treatment of recipient camels with meclofenamic acid reduced the need for tightly timed synchrony between donor and recipient because pregnancies were established in recipients that had ovulated as much as 5 days ahead of the donor.

   
Subscriber Login
Username:
Password:  

    


 
Top  Email this page
 
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2012