Androgen and oestrogen modulation by D-aspartate in rat epididymis
S. Falvo A , M. M. Di Fiore A , L. Burrone A , G. Chieffi Baccari A , S. Longobardi B and A. Santillo A C
+ Author Affiliations
- Author Affiliations
A Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Seconda Università di Napoli, Via Vivaldi 43, 81100, Caserta, Italy.
B Medical Liaison Office, Merck Serono S.p.A., Via Casilina 125, 00176, Roma, Italy.
C Corresponding author. Email: alessandra.santillo@unina2.it
Reproduction, Fertility and Development 28(12) 1865-1872 https://doi.org/10.1071/RD15092
Submitted: 8 March 2015 Accepted: 5 May 2015 Published: 5 June 2015
Abstract
Testosterone (T) synthesised in Leydig cells enters the epididymis and may there be converted into dihydrotestosterone (DHT) by 5α-reductase (5α-red) or into 17β-oestradiol (E2) by P450 aromatase (P450-aro). D-aspartate (D-Asp) is known to induce T synthesis in the testis. In this study, we investigated the effects of in vivo D-Asp administration in two major regions of the rat epididymis (Region I: initial segment, caput, corpus; Region II: cauda). The results suggest that exogenous D-Asp was taken up by both regions of rat epididymis. D-Asp administration induced a rapid increase in T, followed by a more gradual decrease in the T : DHT ratio in Region I. In Region II, T levels rapidly decreased and the T : DHT ratio was consistently lower relative to the control. Expression of 5α-red and androgen receptor genes showed a good correlation with DHT levels in both regions. D-Asp treatment also induced an increase of both E2 levels and oestradiol receptor-α (ERα) expression in Region I, whereas neither E2 levels nor ERα expression were affected in Region II. The early increase of P450-aro expression in Region I and late increase in Region II suggests a direct involvement of D-Asp modulation in P450-aro gene expression. Our results suggest that D-Asp modulates androgen and oestrogen levels and expression of androgen and oestrogen receptors in the rat epididymis by acting on the expression of 5α-red and P450-aro genes.
Additional keywords: 17β-oestradiol, 5α-reductase, testosterone.
References
Assisi, L., Botte, V., D’Aniello, A., and Di Fiore, M. M. (2001). Enhancement of aromatase activity by D-aspartic acid in the ovary of the lizard Podarcis s. sicula. Reproduction 121, 803–808.| Enhancement of aromatase activity by D-aspartic acid in the ovary of the lizard Podarcis s. sicula.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXktVait7g%3D&md5=27bb689d40d90a9489b4ed62777115ecCAS | 11427169PubMed |
Awider-Al-Amawi, M., Marchlewicz, M., Kolasa, A., Wenda-Rozewicka, L., and Wiszniewska, B. (2007). Rat epididymal epithelial cells and 17beta-oestradiol synthesis under hCG stimulation in vitro. Folia Histochem. Cytobiol. 45, 255–263.
| 17951176PubMed |
Bilińska, B., Wiszniewska, B., Kosiniak-Kamysz, K., Kotula-Balak, M., Gancarczyk, M., Hejmej, A., Sadowska, J., Marchlewicz, M., Kolasa, A., and Wenda-Rózewicka, L. (2006). Hormonal status of male reproductive system: androgens and oestrogens in the testis and epididymis. In vivo and in vitro approaches. Reprod. Biol. 6, 43–58.
| 16967089PubMed |
Burrone, L., Raucci, F., and Di Fiore, M. M. (2012a). Steroidogenic gene expression following D-aspartate treatment in frog testis. Gen. Comp. Endocrinol. 175, 109–117.
| Steroidogenic gene expression following D-aspartate treatment in frog testis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVGgsQ%3D%3D&md5=388fb04e86bcd9b2663a55f3136c4a7dCAS | 22036840PubMed |
Burrone, L., Santillo, A., Pinelli, C., Chieffi Baccari, G., and Di Fiore, M. M. (2012b). Induced synthesis of P450 aromatase and 17b-oestradiol by D-aspartate in frog brain. J. Exp. Biol. 215, 3559–3565.
| Induced synthesis of P450 aromatase and 17b-oestradiol by D-aspartate in frog brain.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtV2nu7s%3D&md5=67f75e9586b394768c360a878606e5c8CAS | 22771744PubMed |
Carpino, A., Pezzi, V., Rago, V., Bilinska, B., and Andò, S. (2001). Immunolocalisation of cytochrome P450 aromatase in rat testis during postnatal development. Tissue Cell 33, 349–353.
| Immunolocalisation of cytochrome P450 aromatase in rat testis during postnatal development.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3MvnvVGluw%3D%3D&md5=fc79b73252464057eb6cf74ae412d137CAS | 11521950PubMed |
Carreau, S. (2003). Oestrogens – male hormones? Folia Histochem. Cytobiol. 41, 107–111.
| 1:CAS:528:DC%2BD3sXnvFejsrc%3D&md5=ea5314d4d96a56e58dccf46766228ad3CAS | 13678329PubMed |
Carreau, S., and Hess, R. A. (2010). Oestrogens and spermatogenesis. Philos. Trans. R. Soc. Lond. B Biol. Sci. 365, 1517–1535.
| Oestrogens and spermatogenesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVaisr3K&md5=c0e8e3aca08f21f04cb2be752791a18aCAS | 20403867PubMed |
Cornwall, G. A. (2009). New insights into epididymal biology and function. Hum. Reprod. Update 15, 213–227.
| New insights into epididymal biology and function.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhs1Ogurc%3D&md5=acbf8d9a6bcd122a7f167435c4ef3541CAS | 19136456PubMed |
D’Aniello, A. (2007). D-Aspartic acid: an endogenous amino acid with an important neuroendocrine role. Brain Res. Rev. 53, 215–234.
| D-Aspartic acid: an endogenous amino acid with an important neuroendocrine role.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtlClurk%3D&md5=e55b9cc5980ca71db94553164ede6daaCAS | 17118457PubMed |
D’Aniello, A., D’Onofrio, G., Pischetola, M., D’Aniello, G., Vetere, A., Petrucelli, L., and Fisher, G. H. (1993). Biological role of D-amino acid oxidase and D-aspartate oxidase. Effects of D-amino acids. J. Biol. Chem. 268, 26941–26949.
| 1:CAS:528:DyaK3sXmsVKnu7o%3D&md5=4ebfc068802d5a5488c873dbffdab4d1CAS | 7903300PubMed |
D’Aniello, A., Di Fiore, M. M., Fisher, G., Milone, A., Seleni, A., D’Aniello, S., Perna, A. F., and Ingrosso, D. (2000a). Occurrence of D-aspartic acid and N-methyl-D-aspartic acid in rat neuroendocrine tissues and their role in the modulation of luteinising hormone and growth-hormone release. FASEB J. 14, 699–714.
| 1:CAS:528:DC%2BD3cXisFyhs70%3D&md5=0a0cc016ee70ccb556aa07a72d934933CAS | 10744627PubMed |
D’Aniello, G., Tolino, A., D’Aniello, A., Errico, F., Fisher, G. H., and Di Fiore, M. M. (2000b). The role of D-aspartic acid and N-methyl-D-aspartic acid in the regulation of prolactin release. Endocrinology 141, 3862–3870.
| 1:CAS:528:DC%2BD3cXmvVGmtrg%3D&md5=d633b9434bb28a9ce7d3ae0cb500c2c2CAS | 11014243PubMed |
D’Aniello, G., Ronsini, S., Guida, F., Spinelli, P., and D’Aniello, A. (2005). Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: possible role in reproduction. Fertil. Steril. 84, 1444–1449.
| Occurrence of D-aspartic acid in human seminal plasma and spermatozoa: possible role in reproduction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlaitrnE&md5=f61cce43fb9e79967eab19d8c19fcaf4CAS | 16275242PubMed |
Di Fiore, M. M., Assisi, L., Botte, V., and D’Aniello, A. (1998). D-aspartic acid is implicated in the control of testosterone production by the vertebrate gonad. Studies on the female green frog, Rana esculenta. J. Endocrinol. 157, 199–207.
| D-aspartic acid is implicated in the control of testosterone production by the vertebrate gonad. Studies on the female green frog, Rana esculenta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXkvVyqsro%3D&md5=bae6ad7dd310f429ae9f4e13520bd9a4CAS | 9659282PubMed |
Di Fiore, M. M., Lamanna, C., Assisi, L., and Botte, V. (2008). Opposing effects of D-aspartic acid and nitric oxide on tuning of testosterone production in mallard testis during the reproductive cycle. Reprod. Biol. Endocrinol. 6, 28.
| Opposing effects of D-aspartic acid and nitric oxide on tuning of testosterone production in mallard testis during the reproductive cycle.Crossref | GoogleScholarGoogle Scholar | 18601714PubMed |
Di Fiore, M. M., Santillo, A., and Chieffi Baccari, G. (2014). Current knowledge of D-aspartate in glandular tissues. Amino Acids 46, 1805–1818.
| Current knowledge of D-aspartate in glandular tissues.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXotFGqsro%3D&md5=035506521ac41fef03bb39f86fdf66e7CAS | 24839076PubMed |
Di Giovanni, M., Topo, E., Santillo, A., D’Aniello, A., and Chieffi Baccari, G. (2010). D-Aspartate binding sites in rat Harderian gland. Amino Acids 38, 229–235.
| D-Aspartate binding sites in rat Harderian gland.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVehtbs%3D&md5=b6b94b3a22761d613cbee02cbd663071CAS | 19153642PubMed |
Ezer, N., and Robaire, B. (2003). Gene expression is differentially regulated in the epididymis after orchidectomy. Endocrinology 144, 975–988.
| Gene expression is differentially regulated in the epididymis after orchidectomy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXhslCqsbc%3D&md5=92be65b0ec4034de9e1fb006d188a039CAS | 12586775PubMed |
Fibbi, B., Filippi, S., Morelli, A., Vignozzi, L., Silvestrini, E., Chavalmane, A., De Vita, G., Marini, M., Gacci, M., Manieri, C., Vannelli, G. B., and Maggi, M. (2009). Oestrogens regulate human and rabbit epididymal contractility through the RhoA/Rho-kinase pathway. J. Sex. Med. 6, 2173–2186.
| Oestrogens regulate human and rabbit epididymal contractility through the RhoA/Rho-kinase pathway.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVOmsbnE&md5=1c2c034444da943ad3ca1e8596db761bCAS | 19453906PubMed |
Filippi, S., Luconi, M., Granchi, S., Vignozzi, L., Bettuzzi, S., Tozzi, P., Ledda, F., Forti, G., and Maggi, M. (2002). Oestrogens, but not androgens, regulate expression and functional activity of oxytocin receptor in rabbit epididymis. Endocrinology 143, 4271–4280.
| Oestrogens, but not androgens, regulate expression and functional activity of oxytocin receptor in rabbit epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XotFGrtbk%3D&md5=d134b2088986814f928e62d56d75567aCAS | 12399422PubMed |
Filippi, S., Morelli, A., Vignozzi, L., Vannelli, G. B., Marini, M., Ferruzzi, P., Mancina, R., Crescioli, C., Mondaini, N., Forti, G., Ledda, F., and Maggi, M. (2005). Oxytocin mediates the oestrogen-dependent contractile activity of endothelin-1 in human and rabbit epididymis. Endocrinology 146, 3506–3517.
| Oxytocin mediates the oestrogen-dependent contractile activity of endothelin-1 in human and rabbit epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXntVaiurY%3D&md5=d1fd677557c3675fd6acaaff8908ba08CAS | 15860558PubMed |
French, F. S., and Ritzén, E. M. (1973). Androgen-binding protein in efferent duct fluid of rat testis. J. Reprod. Fertil. 32, 479–483.
| Androgen-binding protein in efferent duct fluid of rat testis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3sXhtlersbY%3D&md5=f4869a040c34af3be12a34fe8fd7d128CAS | 4692344PubMed |
Furuchi, T., and Homma, H. (2005). Free D-aspartate in mammals. Biol. Pharm. Bull. 28, 1566–1570.
| Free D-aspartate in mammals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFWjurzJ&md5=0fcf7ba37fd1e883a25807cacd3bc9a7CAS | 16141517PubMed |
Hamzeh, M., and Robaire, B. (2009). Effect of testosterone on epithelial cell proliferation in the regressed rat epididymis. J. Androl. 30, 200–212.
| Effect of testosterone on epithelial cell proliferation in the regressed rat epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjslKjtbs%3D&md5=acc992d7eeb22bf19ac33c14bd58b1f6CAS | 18930902PubMed |
Heldring, N., Pike, A., Andersson, S., Matthews, J., Cheng, G., Hartman, J., Tujague, M., Ström, A., Treuter, E., Warner, M., and Gustafsson, J. A. (2007). Oestrogen receptors: how do they signal and what are their targets? Physiol. Rev. 87, 905–931.
| Oestrogen receptors: how do they signal and what are their targets?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXptFGls7k%3D&md5=74f82495c4f23f611e4d7d90242e168cCAS | 17615392PubMed |
Henderson, N. A., Cooke, G. M., and Robaire, B. (2006). Region-specific expression of androgen and growth-factor pathway genes in the rat epididymis and the effects of dual 5alpha-reductase inhibition. J. Endocrinol. 190, 779–791.
| Region-specific expression of androgen and growth-factor pathway genes in the rat epididymis and the effects of dual 5alpha-reductase inhibition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFWqtr3J&md5=8dae279caf7cf1d3dd138b2304effaedCAS | 17003279PubMed |
Hess, R. A. (2000). Oestrogen in fluid transport in efferent ducts of the male reproductive tract. Rev. Reprod. 5, 84–92.
| Oestrogen in fluid transport in efferent ducts of the male reproductive tract.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXjsleiu7w%3D&md5=f07b663c6c4847f5725987ff689166e4CAS | 10864852PubMed |
Hess, R. A., Fernandes, S. A., Gomes, G. R., Oliveira, C. A., Lazari, M. F., and Porto, C. S. (2011). Oestrogen and its receptors in efferent ductules and epididymis. J. Androl. 32, 600–613.
| Oestrogen and its receptors in efferent ductules and epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVCru73M&md5=e1df3d36e7612cfe94942f3c3541f0f4CAS | 21441425PubMed |
Igarashi-Migitaka, J., Takeshita, A., Koibuchi, N., Yamada, S., Ohtani-Kaneko, R., and Hirata, K. (2005). Differential expression of p160 steroid receptor co-activators in the rat testis and epididymis. Eur. J. Endocrinol. 153, 595–604.
| Differential expression of p160 steroid receptor co-activators in the rat testis and epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFygsb7J&md5=86e23c02cd8cac17c65fb35958c06cebCAS | 16189181PubMed |
Jänne, O. A., Moilanen, A. M., Poukka, H., Rouleau, N., Karvonen, U., Kotaja, N., Häkli, M., and Palvimo, J. J. (2000). Androgen receptor-interacting nuclear proteins. Biochem. Soc. Trans. 28, 401–405.
| Androgen receptor-interacting nuclear proteins.Crossref | GoogleScholarGoogle Scholar | 10961928PubMed |
Joseph, A., Shur, B. D., and Hess, R. A. (2011). Oestrogen, efferent ductules and the epididymis. Biol. Reprod. 84, 207–217.
| Oestrogen, efferent ductules and the epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVelsbo%3D&md5=24a1ec3e667634d31c23d224ad6f3481CAS | 20926801PubMed |
Lamanna, C., Assisi, L., Botte, V., and Di Fiore, M. M. (2006). Endogenous testicular D-aspartic acid regulates gonadal aromatase activity in boar. J. Endocrinol. Invest. 29, 141–146.
| Endogenous testicular D-aspartic acid regulates gonadal aromatase activity in boar.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XkvFGgs7o%3D&md5=f765324518e5fc155d276f80b0f61f9aCAS | 16610240PubMed |
Lamanna, C., Assisi, L., Botte, V., and Di Fiore, M. M. (2007). Involvement of D-Asp in P450 aromatase activity and oestrogen receptors in boar testis. Amino Acids 32, 45–51.
| Involvement of D-Asp in P450 aromatase activity and oestrogen receptors in boar testis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Ogs7vM&md5=8d9e39e82c1a00691c9a217945f1844dCAS | 17469225PubMed |
Lamb, D. J., Weigel, N. L., and Marcelli, M. (2001). Androgen receptors and their biology. Vitam. Horm. 62, 199–230.
| Androgen receptors and their biology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXktlenu7g%3D&md5=d1bd74aac2f3f43829e1c737398521dcCAS | 11345899PubMed |
Li, J., and Al-Azzawi, F. (2009). Mechanism of androgen receptor action. Maturitas 63, 142–148.
| Mechanism of androgen receptor action.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXnsV2ks78%3D&md5=6977b81d318def5b5d71354ebfadd81fCAS | 19372015PubMed |
Monteforte, R., Santillo, A., Di Giovanni, M., D’Aniello, A., Di Maro, A., and Chieffi Baccari, G. (2009). D-Aspartate affects secretory activity in rat Harderian gland: molecular mechanism and functional significance. Amino Acids 37, 653–664.
| D-Aspartate affects secretory activity in rat Harderian gland: molecular mechanism and functional significance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtFyqt7vK&md5=6732b822b70ddd6a74f0f6a245c9e330CAS | 18820994PubMed |
Nagata, Y., Homma, H., Lee, J.-A., and Imai, K. (1999a). D-Aspartate stimulation of testosterone synthesis in rat Leydig cells. FEBS Lett. 444, 160–164.
| D-Aspartate stimulation of testosterone synthesis in rat Leydig cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhtFaqs7c%3D&md5=df3d35ce2f8b61be714d1fefe6a48e4fCAS | 10050750PubMed |
Nagata, Y., Homma, H., Matsumoto, M., and Imai, K. (1999b). Stimulation of steroidogenic acute regulatory (StAR) gene expression by D-aspartate in rat Leydig cells. FEBS Lett. 454, 317–320.
| Stimulation of steroidogenic acute regulatory (StAR) gene expression by D-aspartate in rat Leydig cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXks1Olsrk%3D&md5=0a5315e00078e371beeb9e1e000688bcCAS | 10431830PubMed |
Patrão, M. T., Silva, E. J., and Avellar, M. C. (2009). Androgens and the male reproductive tract: an overview of classical roles and current perspectives. Arq. Bras. Endocrinol. Metabol. 53, 934–945.
| Androgens and the male reproductive tract: an overview of classical roles and current perspectives.Crossref | GoogleScholarGoogle Scholar | 20126845PubMed |
Pereyra-Martinez, A. C., Roselli, C. E., Stadelman, H. L., and Resko, J. A. (2001). Cytochrome P450 aromatase in testis and epididymis of male rhesus monkeys. Endocrine 16, 15–19.
| Cytochrome P450 aromatase in testis and epididymis of male rhesus monkeys.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjtVelsA%3D%3D&md5=77bca6624c9003419d4aa22b91dacbe3CAS | 11822822PubMed |
Raucci, F., and Di Fiore, M. M. (2011). D-Asp: a new player in reproductive endocrinology of the amphibian Rana esculenta. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 879, 3268–3276.
| D-Asp: a new player in reproductive endocrinology of the amphibian Rana esculenta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlKrtb%2FK&md5=7188228835ab18be21ada9181d364b0cCAS | 21531634PubMed |
Raucci, F., Assisi, L., D’Aniello, S., Spinelli, P., Botte, V., and Di Fiore, M. M. (2004). Testicular endocrine activity is upregulated by D-aspartic acid in the green frog, Rana esculenta. J. Endocrinol. 182, 365–376.
| Testicular endocrine activity is upregulated by D-aspartic acid in the green frog, Rana esculenta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXntFOgtb8%3D&md5=c2ded03eebde3fab522c238e48b03129CAS | 15283696PubMed |
Raucci, F., D’Aniello, S., and Di Fiore, M. M. (2005). Endocrine roles of D-aspartic acid in the testis of lizard Podarcis s. sicula. J. Endocrinol. 187, 347–359.
| Endocrine roles of D-aspartic acid in the testis of lizard Podarcis s. sicula.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XitlOrtA%3D%3D&md5=d343ba0ca8be24097139d5687f1ea00bCAS | 16423814PubMed |
Raucci, F., D’Aniello, A., and Di Fiore, M. M. (2014). Stimulation of androgen production by D-aspartate through the enhancement of StAR, P450scc and 3b-HSD mRNA levels in in vivo rat testis and in culture of immature rat Leydig cells. Steroids 84, 103–110.
| Stimulation of androgen production by D-aspartate through the enhancement of StAR, P450scc and 3b-HSD mRNA levels in in vivo rat testis and in culture of immature rat Leydig cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXntl2msbg%3D&md5=0bfc92b65160718fa522b89d127c3203CAS | 24713504PubMed |
Robaire, B., and Hamzeh, M. (2011). Androgen action in the epididymis. J. Androl. 32, 592–599.
| Androgen action in the epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVCru7zF&md5=c6baac32d7ef383dc3549d4fb3046968CAS | 21764895PubMed |
Robaire, B., and Henderson, N. A. (2006). Actions of 5alpha-reductase inhibitors on the epididymis. Mol. Cell. Endocrinol. 250, 190–195.
| Actions of 5alpha-reductase inhibitors on the epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XksVGjsbk%3D&md5=1f8724640788138abadf0eb3a395b2afCAS | 16476520PubMed |
Robaire, B., Seenundun, S., Hamzeh, M., and Lamour, S. A. (2007). Androgenic regulation of novel genes in the epididymis. Asian J. Androl. 9, 545–553.
| Androgenic regulation of novel genes in the epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXptVSlt70%3D&md5=08123e94adf066e6dbf831654cbff532CAS | 17589794PubMed |
Sakai, K., Homma, H., Lee, J. A., Fukushima, T., Santa, T., Tashiro, K., Iwatsubo, T., and Imai, K. (1998). Localisation of D-aspartic acid in elongate spermatids in rat testis. Arch. Biochem. Biophys. 351, 96–105.
| Localisation of D-aspartic acid in elongate spermatids in rat testis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXhs1Shurg%3D&md5=7ad19b61ba24655810994ffbccea3166CAS | 9500846PubMed |
Santillo, A., Burrone, L., Senese, R., Cioffi, F., Lanni, A., and Chieffi Baccari, G. (2011). Effect of D-aspartate uptake on uncoupling protein-3 and α-tubulin expressions in rat Harderian gland. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 879, 3344–3348.
| Effect of D-aspartate uptake on uncoupling protein-3 and α-tubulin expressions in rat Harderian gland.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlKrtbzL&md5=ba07b9944f22a621a46c4c1deea2d744CAS | 21524945PubMed |
Santillo, A., Pinelli, C., Burrone, L., Chieffi Baccari, G., and Di Fiore, M. M. (2013). D-Aspartic acid implication in the modulation of frog brain sex-steroid levels. Gen. Comp. Endocrinol. 181, 72–76.
| D-Aspartic acid implication in the modulation of frog brain sex-steroid levels.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhslynurfN&md5=3fd38a77aef057e4050aeda118e4c8cfCAS | 23153651PubMed |
Santillo, A., Falvo, S., Chieffi, P., Burrone, L., Chieffi Baccari, G., Longobardi, S., and Di Fiore, M. M. (2014). D-Aspartate affects NMDA receptor-extracellular signal-regulated kinase pathway and upregulates androgen receptor expression in the rat testis. Theriogenology 81, 744–751.
| D-Aspartate affects NMDA receptor-extracellular signal-regulated kinase pathway and upregulates androgen receptor expression in the rat testis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtlSmsbo%3D&md5=c523a1f2dca34d6de3fbc0a67c854407CAS | 24439162PubMed |
Shayu, D., Hardy, M. P., and Rao, A. J. (2007). Delineating the role of oestrogen in regulating epididymal gene expression. Soc. Reprod. Fertil. Suppl. 63, 31–43.
| 1:CAS:528:DC%2BD1cXpvVyksLs%3D&md5=c1cb3ecf534f9c730145b3ae956a1c85CAS | 17566259PubMed |
Stanišić, V., Lonard, D. M., and O’Malley, B. W. (2010). Modulation of steroid hormone receptor activity. Prog. Brain Res. 181, 153–176.
| Modulation of steroid hormone receptor activity.Crossref | GoogleScholarGoogle Scholar | 20478437PubMed |
Topo, E., Soricelli, A., D’Aniello, A., Ronsini, S., and D’Aniello, G. (2009). The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats. Reprod. Biol. Endocrinol. 7, 120.
| The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats.Crossref | GoogleScholarGoogle Scholar | 19860889PubMed |
Trybek, G., Kolasa, A., Marchlewicz, M., Wenda-Rozewicka, L., and Wiszniewska, B. (2005). Immunolocalisation of androgen receptor in the epididymis of rats with dihydrotestosterone deficiency. Reprod. Biol. 5, 291–301.
| 16372046PubMed |
Wiszniewska, B. (2002). Primary culture of the rat epididymal epithelial cells as a source of oestrogen. Andrologia 34, 180–187.
| Primary culture of the rat epididymal epithelial cells as a source of oestrogen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XltlOqtrY%3D&md5=a6974212c90681c9ece63c56db49a315CAS | 12059815PubMed |
Yamashita, S. (2004). Localisation of oestrogen and androgen receptors in male reproductive tissues of mice and rats. Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 279A, 768–778.
| Localisation of oestrogen and androgen receptors in male reproductive tissues of mice and rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXnt1Ont74%3D&md5=741013fd22254f53ff08a3c139b8e604CAS |
Zaya, R., Hennick, C., and Pearl, C. A. (2012). In vitro expression of androgen and oestrogen receptors in prepubertal and adult rat epididymis. Gen. Comp. Endocrinol. 178, 573–586.
| In vitro expression of androgen and oestrogen receptors in prepubertal and adult rat epididymis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht1GksbnI&md5=24fd4c5ab55348a1221412106eaef88eCAS | 22809666PubMed |
Zhu, L. J., Hardy, M. P., Inigo, I. V., Huhtaniemi, I., Bardin, C. W., and Moo-Young, A. J. (2000). Effects of androgen on androgen receptor expression in rat testicular and epididymal cells: a quantitative immunohistochemical study. Biol. Reprod. 63, 368–376.
| Effects of androgen on androgen receptor expression in rat testicular and epididymal cells: a quantitative immunohistochemical study.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXltl2gt70%3D&md5=d612d881188d7d8a5478c963c018aaaaCAS | 10906039PubMed |
