Site-specific transgene insertion: an approach

P Wigley, C Becker, J Beltrame, T Blake, L Crocker, S Harrison, I Lyons, Z McKenzie, R Tearle, R Crawford and al et

Abstract

Methods to improve the production of transgenic animals are being developed. Conventional transgenesis, involving microinjection of DNA into fertilized eggs, has a number of limitations. These result from the inability to control both the site of transgene insertion and the number of gene copies inserted. The approach described seeks to overcome these problems and to allow single copy insertion of transgenes into a defined site in animal genomes. The method involves the use of embryonic stem cells, gene targeting and the FLP recombinase system.

Reproduction, Fertility and Development 6(5) 585 - 588 (1994) doi:10.1071/RD9940585