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Table of Contents     |     Next Issue >>   Reproduction, Fertility and Development Volume 19 Number 1 2007 Proceedings of the Annual Conference of the International Embryo Transfer Society, Kyoto, Japan, 6-10 January 2007 Full Papers and Abstracts for Poster Presentation IETS 2007 author index  pp. 325-334 PDF (101 KB)   IETS 2007 abstracts  pp. 119-324 PDF (1.9 MB) - $50.00        Recipient of the 2007 IETS Pioneer Award: Akira Iritani, PhD  pp. xxxi-xxxi PDF (112 KB)   Evaluation of oocyte quality: morphological, cellular and molecular predictors  Qiang Wang and Qing-Yuan Sun pp. 1-12 Abstract     |    PDF (197 KB) - $25.00        Oocyte cryopreservation: oocyte assessment and strategies for improving survival  Sergio Ledda, Luisa Bogliolo, Sara Succu, Federica Ariu, Daniela Bebbere, Giovanni Giuseppe Leoni and Salvatore Naitana pp. 13-23 Abstract     |    PDF (1.2 MB) - $25.00        Maternally derived transcripts: identification and characterisation during oocyte maturation and early cleavage  Xiang-Shun Cui and Nam-Hyung Kim pp. 25-34 Abstract     |    PDF (204 KB) - $25.00        Temporal and spatial control of gene expression in early embryos of farm animals  Tiziana A. L. Brevini, Fabiana Cillo, Stefania Antonini, Valentina Tosetti and Fulvio Gandolfi pp. 35-42 Abstract     |    PDF (221 KB) - $25.00        Embryo culture and long-term consequences  Jeremy G. Thompson, Megan Mitchell and Karen L. Kind pp. 43-52 Abstract     |    PDF (1 MB) - $25.00        Long-term effects of nutritional programming of the embryo and fetus: mechanisms and critical windows  Michael E. Symonds, Terence Stephenson, David S. Gardner and Helen Budge pp. 53-63 Abstract     |    PDF (209 KB) - $25.00        Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, interferons and endogenous retroviruses  Thomas E. Spencer, Greg A. Johnson, Fuller W. Bazer, Robert C. Burghardt and Massimo Palmarini pp. 65-78 Abstract     |    PDF (574 KB) - $25.00        Gene expression and maintenance of pregnancy in bovine: roles of trophoblastic binucleate cell-specific molecules  Kazuyoshi Hashizume, Koichi Ushizawa, Osman V. Patel, Keiichiro Kizaki, Kei Imai, Osamu Yamada, Haruo Nakano and Toru Takahashi pp. 79-90 Abstract     |    PDF (295 KB) - $25.00        State of the art in farm animal sperm evaluation  H. Rodríguez-Martínez pp. 91-101 Abstract     |    PDF (203 KB) - $25.00        Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction  S. S. Suarez pp. 103-110 Abstract     |    PDF (279 KB) - $25.00        Stem cells and lineage development in the mammalian blastocyst  Janet Rossant pp. 111-118 Abstract     |    PDF (173 KB) - $25.00        1 XENOGRAFTING OF ADULT MAMMALIAN TESTIS TISSUE   L. Arregui, R. Rathi, W. Zeng, A. Honaramooz, M. Gomendio, E.R.S Roldan and I. Dobrinski pp. 119-119 Abstract      2 GLOBAL PEPTIDE SEQUENCING AND QUANTIFICATION OF PROTEINS IN PORCINE PARTHENOTES BY PROTEOMICS   J. M. Jang, M. K. Gupta, J. W. Jung, S. J. Uhm, K. P. Kim and H. T. Lee pp. 119-119 Abstract      3 HISTONE H4 ACETYLATION AT LYSINE 12 AND Cdc2a EXPRESSION ARE DECREASED IN AGED MOUSE GERMINAL VESICLE-STAGE OOCYTES  I. Manosalva, C. Goday and P. Esponda pp. 120-120 Abstract      4 MYOSTATIN GENE KNOCKDOWN THROUGH LENTIVIRAL VECTOR-MEDIATED DELIVERY OF shRNA FOR IN VITRO PRODUCTION OF TRANSGENIC BOVINE EMBRYOS  M. P. Milazzotto, W. B. Feitosa, B. E. Strauss, M. Bajgelman, C. M. Mendes, M. E. O. A. Assumpção and J. A. Visintin pp. 120-120 Abstract      5 NUCLEOLAR DEVELOPMENT REQUIRES TRANSCRIPTIONAL ACTIVITY DURING PORCINE EMBRYONIC GENOME ACTIVATION  O. Svarcova, P. Maddox-Hyttel, H. Niemann, D. Hermann, Z. Rasmussen, I. Petrovicova, F. Strejcek and J. Laurincik pp. 120-121 Abstract      6 TIMING OF DEADENYLATION OF GDF-9 AND CYCLIN 3′ UTR CONSTRUCTS IN BOVINE OOCYTES   D. J. Walker, C. J. Wilusz and G. E. Seidel Jr pp. 121-121 Abstract      7 EFFECTS OF DIFFERENT TRYPSIN SOURCES AND CONCENTRATIONS ON THE VIABILITY OF BOVINE SPERM PRE- AND POST-CRYOPRESERVATION  B. A. Blevins, S. Steenson and N. M. Loskutoff pp. 121-122 Full Text      8 PREGNANCY RATE FOLLOWING TIMED AI IN BEEF HEIFERS TREATED WITH CUE-MATE AND pLH OR GnRH  M. G. Colazo, F. C. Dias, K. Lightfoot, O. Dochi, J. P. Kastelic and R. J. Mapletoft pp. 122-122 Abstract      9 CAN ONE PREDICT THE RESISTANCE OF BULL SPERM TO CRYOPRESERVATION BY ANALYZING MOTILITY PARAMETERS BEFORE FREEZING?   L. Defoin, A. Granados and I. Donnay pp. 123-123 Abstract      10 EFFECTS OF INSEMINATION TIME, BREED, AND INSEMINATOR ON FERTILITY OF EWES INTRAUTERINALLY INSEMINATED WITH FROZEN–THAWED SEMEN IMPORTED FROM NEW ZEALAND   Y. Fukui, H. Kohno, T. Togari, T. Matsuoka and H. Imai pp. 123-123 Abstract      11 FERTILITY OF FRESH AND FROZEN–THAWED EPIDIDYMAL STALLION SPERM WITH OR WITHOUT EXPOSURE TO SEMINAL PLASMA  A. Heise, D. Gerber, D. H. Volkmann, W. Kähn and N. K. Brouwer pp. 124-124 Full Text      12 GENERATION OF RAT OFFSPRING DERIVED FROM CRYOPRESERVED SPERMATOZOA IN JAPANESE NATIONAL BIORESOURCES  N. Kashiwazaki, Y. Seita, K. Naoi, A. Takizawa, T. Kuramoto and T. Serikawa pp. 124-125 Abstract      13 MANAGEMENT SYSTEM FOR FROZEN BULL SEMEN USING A RADIO FREQUENCY IDENTIFICATION TAG  S. Kato, K. Mukojima, T. Otani, K. Sakai, K. Tamura, K. Miyazaki, N. Minami and H. Imai pp. 125-125 Abstract      14 MOTILITY AND FERTILITY OF BULL SPERMATOZOA FROZEN IN EGG YOLK EXTENDER SUPPLEMENTED WITH LACTOFERRIN   J. Kobayashi, Y. Suda, N. Takada, K. Komine, T. Kuroishi, T. Kohsaka, H. Sasada and E. Sato pp. 125-126 Abstract      15 EFFECT OF BULL EPIDIDYMIS STORAGE CONDITIONS ON CRYOPRESERVED EPIDIDYMAL SPERM IN VITRO FERTILITY AND LIPID PEROXIDATION STATUS   M. Nichi, T. Rijsselaere, A. Van Soom, J. B. P. De Clercq, I. G. F. Goovaerts, V. H. Barnabe and P. E. J. Bols pp. 126-126 Abstract      16 PROGESTERONE RELEASE PATTERNS FROM CUE-MATE IN COMPARISON TO OTHER INTRAVAGINAL PROGESTERONE-RELEASING DEVICES IN LACTATING DAIRY COWS   D. Rogan, M. F. Martinez, G. A. Bo, P. Chesta, F. Feresin and R. J. Mapletoft pp. 126-127 Abstract      17 WHITETAIL DEER OFFSPRING FROM ARTIFICIAL INSEMINATION WITH EPIDIDYMAL SPERM HARVESTED FROM A HUNTER-KILLED BUCK   J. R. Saenz, G. T. Gentry, W. Forbes, B. Olcott, J. Chenevert, K. R. Bondioli, D. E. Sanders and R. A. Godke pp. 127-127 Abstract      18 THE EFFECTS OF CIDR AND eCG TREATMENTS IN A GnRH-BASED PROTOCOL FOR TIMED AI OR EMBRYO TRANSFER ON PREGNANCY RATES IN LACTATING BEEF COWS   J. A. Small, M. G. Colazo, J. P. Kastelic, D. R. Ward, O. Dochi and R. J. Mapletoft pp. 127-128 Abstract      19 EFFECTS OF ESTRADIOL OR GnRH ON FOLLICULAR WAVE EMERGENCE AND PREGNANCY RATES FOR A CIDR-BASED TIMED AI PROTOCOL IN REPEAT BREEDER DAIRY COWS   G. H. Suh, U. H. Kim, T. Y. Hur, S. J. Kang, K. S. Baek, S. B. Park, H. S. Kim, H. G. Kang and I. H. Kim pp. 128-128 Abstract      20 SPERM CHARACTERIZATION OF ASTURCON PONIES AFTER COLLECTION AND EQUILIBRATION/REFRIGERATION BEFORE FREEZING   C. Tamargo, C. Díez, J. De La Fuente, M. Carbajo, J. M. Benito and C. O. Hidalgo pp. 128-129 Abstract      21 RELATIONSHIP BETWEEN DURATION OF ESTRUS BEHAVIOR DETERMINED BY RADIOTELEMETORY SYSTEM AND THREE FACTORS (MILK PRODUCTION, STEROID HORMONE, AND OVULATION TIME) IN DAIRY COWS  H. Yoshioka, H. Matsuda, K. Sasaguri and M. Yonai pp. 129-129 Abstract      22 RESPIRATORY ACTIVITY AND ULTRASTRUCTURAL FEATURES OF BOVINE SOMATIC NUCLEAR TRANSFER EMBRYOS  H. Abe, K. Aoyagi, S. Aoyagi, H. Shiku, T. Matsue, Y. Sendai and H. Hoshi pp. 129-130 Abstract      23 HYPOMETHYLATION OF DNA IN NUCLEAR TRANSFER EMBRYOS FROM PORCINE EMBRYONIC GERM CELLS   K. S. Ahn, S. Y. Heo, J. Y. Won and H. Shim pp. 130-130 Abstract      24 EFFECT OF TREATMENT WITH TRICHOSTATIN A ON IN VITRO DEVELOPMENT OF BOVINE NUCLEAR-TRANSFERRED EMBRYOS  S. Akagi, K. Fukunari, K. Matsukawa, S. Watanabe and S. Takahashi pp. 130-131 Full Text      25 THE EFFECTS OF OOCYTE SOURCES AND ACTIVATION PROTOCOLS ON THE DEVELOPMENT OF CLONED GOAT EMBRYOS   M. Apimeteeumrong, A. Thuangsanthia, N. Leingchaloen, V. Yiengvisavakul, A. Harintharanon, A. Kunavongkrit, X. Vignon and M. Techakumphu pp. 131-131 Abstract      26 SPERM REPRODUCTIVE POTENTIAL OF FETAL FIBROBLAST CELLS ON A NUCLEAR TRANSFER–CLONED BULL AND ON AN ORIGINAL PATERNAL BULL: A COMPARATIVE ANALYSIS   S.-H. Bae, D.-H. Kim, S.-W. Kim, G.-S. Im, M.-J. Lee, J.-W. Ryu, H.-H. Seong, B.-S. Yang and B.-C. Yang pp. 131-132 Abstract      27 CYTOPLASM OF A GERMINAL VESICLE OOCYTE CAN PROMOTE SOMATIC NUCLEAR REPROGRAMMING IN MICE  H.T. Bui, N. Van Thuan, S. Kishigami, S. Wakayama, T. Hikichi, H. Ohta, E. Mizutani and T. Wakayama pp. 132-132 Abstract      28 EXPRESSION OF MHC CLASS I IN THE PLACENTA AND THE LYMPHOCYTES OF BOVINE SOMATIC CLONES  P. Chavatte-Palmer, M. Guillomot, J. Roïz, J.-L. Servely, Y. Heyman, I. Hue and S. Ellis pp. 132-133 Abstract      29 COMPARISON OF DNA METHYLATION RATES BETWEEN CLONED CATS AND DOMESTIC CATS  S. J. Cho, S. H. Kang, S. G. Cho, I. H. Bae, C. J. Yang, X. J. Yin and I. K. Kong pp. 133-133 Abstract      30 GROWTH AND REPRODUCTIVE SUCCESS OF CLONED MALE CATS DERIVED FROM ADULT SOMATIC CELL NUCLEAR TRANSFER   E. G. Choi, X. J. Yin, H. S. Lee, S. H. Kang, Y. J. Lee, L. H. Kim, H. D. Shin, N. H. Kim and I. K. Kong pp. 133-134 Abstract      31 EFFECT OF TREATMENT OF OVINE CYTOPLASTS WITH CAFFEINE ON THE TRANSLOCALIZATION OF HSP27 IN SOMATIC CELL NUCLEAR TRANSFER RECONSTRUCTED EMBRYOS  I. Choi, J.-H. Lee and K. H. S. Campbell pp. 134-134 Abstract      32 EFFECTS OF FUSION/ACTIVATION METHODS ON DEVELOPMENT OF EMBRYOS PRODUCED BY NUCLEAR TRANSFER OF PORCINE FETAL FIBROBLASTS   P. Q. Cong, E. S. Song, E. S. Kim, Z. H. Li, Y. J. Yi and C. S. Park pp. 134-135 Abstract      33 ENUCLEATION OF PRE-ACTIVATED MOUSE OOCYTES INDUCED BY DEMECOLCINE, NOCODAZOLE, AND VINBLASTINE  N. Costa-Borges, J. Santaló and E. Ibàñez pp. 135-135 Abstract      34 PIGLETS BORN FROM HANDMADE CLONING   Y. Du, Y. Zhang, J. Li, P. M. Kragh, M. Schmidt, I. B. Bøgh, X. Zhang, S. Purup, A. L. Jørgensen, A. M. Pedersen, K. Villemoes, H. Yang, L. Bolund and G. Vajta pp. 135-136 Abstract      35 RELATIONSHIP BETWEEN THE ONSET OF ENUCLEATION DURING MEIOTIC MATURATION OF RECIPIENT OOCYTES AND DEVELOPMENTAL ABILITY OF SOMATIC CELL NUCLEAR TRANSFER EMBRYOS IN PIGS   M. Fahrudin, K. Kikuchi, N. W. K. Karja, M. Ozawa, T. Somfai, M. Nakai, K. Ohnuma, J. Noguchi, H. Kaneko and T. Nagai pp. 136-136 Abstract      36 EPIGENETIC MODIFICATIONS OF CULTURED BOVINE FIBROBLASTS FOR NUCLEAR TRANSFER   A. M. Giraldo, J. W. Lynn, M. N. Purpera, R. A. Godke and K. R. Bondioli pp. 136-137 Abstract      37 ANATOMICAL DEVIATIONS IN APPARENTLY HEALTHY, WEANED, CLONED CALVES   M. P. Green, M. C. Berg and R. S. F. Lee pp. 137-137 Abstract      38 RABBIT NUCLEAR TRANSFER AND IN VIVO-FERTILIZED EMBRYOS FAIL TO EXPRESS A MOUSE Oct-4 PROMOTER-DRIVEN EGFP REPORTER GENE  R. Hao, A. Wuensch, R. Klose, E. Wolf and V. Zakhartchenko pp. 137-138 Abstract      39 BOVINE SOMATIC CELL NUCLEAR TRANSFER USING CUMULUS–OOCYTE COMPLEXES COLLECTED FROM THE IDENTICAL INDIVIDUAL BY OVUM PICKUP  K. Hasegawa, S. Takahashi, S. Akagi, K. Takeda, K. Imai, M. Shimizu, T. Okazaki, S. Abe and Y. Izaike pp. 138-138 Full Text      40 TREATMENT OF DONOR CELLS AND ITS EFFECT ON INTERSPECIES NUCLEAR TRANSFER   M. A. Hashem, D. P. Bhandari, S. K. Kang and B. C. Lee pp. 138-139 Abstract      41 TRANSMISSION OF A PARASITE-RESISTANT PHENOTYPE FROM A DONOR RAM TO ITS CLONED OFFSPRING   J. Hill, W. Chang, R. Davey, K. Hutton and G. Riding pp. 139-139 Abstract      42 PREPARTUM HORMONAL CHANGES IN RECIPIENT COWS FOR SOMATIC CELL CLONED FETUSES   H. Hirayama, K. Sawai, S. Moriyasu, S. Kageyama, S. Onoe, M. Hirayama, Y. Goto, E. Kaneko, A. Miyamoto and A. Minamihashi pp. 139-140 Abstract      43 INFLUENCE OF DONOR CELL DIFFERENTIATION ON CLONING EFFICIENCY IN PIGS   N. Hornen, W. A. Kues, A. Lucas-Hahn, B. Petersen, P. Hassel and H. Niemann pp. 140-141 Abstract      44 NUCLEAR TRANSFER IN CATTLE USING SOMATIC CELLS FROM FROZEN TESTICLES WITHOUT CRYOPROTECTANTS  Y. Hoshino, N. Kobayashi, N. Hayashi, T. Matsuhashi, K. Saeki, S. Ikeda, S. Taniguchi, A. Kasamatsu, D. Iwamoto, Y. Abe, K. Matsumoto, Y. Hosoi and A. Iritani pp. 140-141 Abstract      45 EFFECT OF OSMOLARITY IN CULTURE MEDIUM ON THE PRE-IMPLANTATION DEVELOPMENT OF PORCINE NT AND IVF EMBRYOS   I. S. Hwang, H. J. Moon, J. H. Shim, M. R. Park, D. H. Kim, B. C. Yang, Y. G. Ko, B. S. Yang, H. T. Cheong and G. S. Im pp. 141-141 Abstract      46 EFFECT OF MANIPULATION MEDIUM ON THE DEVELOPMENT OF RECONSTRUCTED DOMESTIC CAT EMBRYOS  S. Imsoonthornruksa, C. Lorthongpanich, K. Srirattana, N. Sripunya, C. Laowtammathron, M. Ketudat-Cairns and R. Parnpai pp. 141-141 Full Text      47 DIFFERENTIAL DEVELOPMENTAL ABILITY OF EMBRYOS CLONED FROM TISSUE-SPECIFIC STEM CELLS  K. Inoue, N. Ogonuki, H. Miki, S. Noda, S. Inoue, K. Katayama, K. Mekada, H. Miyoshi and A. Ogura pp. 142-142 Abstract      48 EFFECTS OF TRICHOSTATIN A ON DEVELOPMENT OF BOVINE SOMATIC CELL NUCLEAR TRANSFER EMBRYOS   D. Iwamoto, K. Saeki, S. Kishigami, A. Kasamatsu, A. Tatemizo, Y. Abe, S. Ikeda, S. Taniguchi, T. Mitani, H. Kato, K. Matsumoto, Y. Hosoi, T. Wakayama and A. Iritani pp. 142-142 Abstract      49 TRANSCRIPT LEVEL OF mRNA IN BOVINE CLONED OR RE-CLONED EMBRYOS  G. Jang, H. Y. Jeon, K. H. Ko, H. J. Oh, H. J. Park, S. G. Hong, J. J. Kim, S. K. Kang and B. C. Lee pp. 142-143 Abstract      50 DNA METHYLATION PROFILES OF UPSTREAM ELEMENTS OF Oct-3/4 GENE IN IN VITRO FERTILIZATION (IVF) AND SOMATIC CELL NUCLEAR-TRANSFERRED (SCNT) EMBRYOS   M. Kawasumi, Y. Unno, M. Nishiwaki, K. Matsumoto, M. Anzai, T. Amano, T. Mitani, H. Kato, K. Saeki, Y. Hosoi and A. Iritani pp. 143-143 Abstract      51 PROTEOMICS ANALYSIS OF PLACENTOMEGALY IN CLONED MICE  H. R. Kim, K. Naruse, H. R. Lee, T. Wakayama, C. S. Park and D. I. Jin pp. 143-144 Abstract      52 EFFECT OF DONOR CELLS WITH VARIOUS DIFFERENTIATED STATUS ON THE DEVELOPMENTAL COMPETENCE OF PORCINE NUCLEAR TRANSFER EMBRYOS  J. G. Kim, E. J. Kang, M. K. Kim, S. Y. Choe and G. J. Rho pp. 144-144 Full Text      53 EFFECTS OF INSULIN-TRANSFERRIN-SELENIUM IN DEFINED AND PORCINE FOLLICULAR FLUID-SUPPLEMENTED MEDIUM ON IN VITRO PRODUCTION OF PORCINE SCNT EMBRYOS   Y. U. Kim, D. P. Bhandari, M. S. Hossein, S. M. Park, E. Lee, S. W. Park, Y. I. Jeong, J. Y. Lee, J. H. Kim, Y. W. Jeong, S. Kim and W. S. Hwang pp. 144-145 Abstract      54 SUCCESS OF MOUSE CLONING FROM AN OUTBRED STRAIN BY TRICHOSTATIN A TREATMENT AFTER SOMATIC NUCLEAR TRANSFER  S. Kishigami, H. Bui, S. Wakayama, N. Van Thuan, T. Hikichi, E. Mizutani, H. Ohta, R. Suetsugu and T. Wakayama pp. 145-145 Abstract      55 DIFFERENTIAL TRANSCRIPTIONAL GENE EXPRESSION BETWEEN MINIATURE AND LANDRACE PIG FETUSES: IMPLICATION FOR PRODUCTION OF CLONED MINIATURE PIGS USING LANDRACE PIGS AS OOCYTE DONORS AND SURROGATE MOTHERS   O. J. Koo, S. H. Lee, M. S. Hossein, S. K. Kang, D. Y. Kim and B. C. Lee pp. 145-145 Abstract      56 TRANSGENESIS BY HANDMADE CLONING USING EGFP-TRANSFECTED YUCATAN FIBROBLASTS  P. M. Kragh, Y. Du, J. Li, Y. Zhang, L. Bolund and G. Vajta pp. 146-146 Abstract      57 NO BENEFICIAL EFFECT OF AGGREGATION ON INTERFERON TAU ACTIVITY IN BOVINE CLONE OUTGROWTHS  S. Kurosaka, A. D. Ealy and K. J. McLaughlin pp. 146-146 Abstract      58 EFFECTS OF CAFFEINE AND VANADATE ON NUCLEAR REMODELING AND MICROTUBULE DISTRIBUTION OF PORCINE NUCLEAR TRANSFER EMBRYOS   D. J. Kwon, C. K. Park, B. K. Yang, C. I. Kim and H. T. Cheong pp. 146-147 Abstract      59 HIGHER BLASTOCYST FORMATION OF SOMATIC CELL NUCLEAR TRANSFER EMBRYOS DOES NOT GUARANTEE BETTER PREGNANCY IN PIG  E. Lee, K. Song, Y. Jeong and S. Hyun pp. 147-147 Full Text      60 IMPROVEMENT IN EFFICIENCY AND QUALITY OF CLONED AND PARTHENOGENETIC PORCINE EMBRYOS BY AMINO ACIDS   H. T. Lee, J. M. Jang, S. H. Lee and M. K. Gupta pp. 147-148 Abstract      61 EFFICIENCY OF TWO ENUCLEATION METHODS FOR THE PRODUCTION OF TRANSGENIC PIG EMBRYOS BY HANDMADE CLONING  J. Li, Y.-H. Zhang, Y.-T. Du, P. M. Kragh, S. Purup, A. M. Pederson, K. Villemoes, A. L. Jørgensen, L. Bolund, Q.-Z. Xue, H.-M. Yang and G. Vajta pp. 148-148 Full Text      62 EXPRESSION AND DISTRIBUTION OF Oct-4 IN INTERSPECIES-CLONED LONG-TAILED MONKEY (MACACA FASCICULARIS) EMBRYOS  C. Lorthongpanich, K. Srirattana, S. Imsoonthornruksa, N. Sripunya, C. Laowtammathron, O. Kumpong, M. Ketudat-Cairns and R. Parnpai pp. 149-149 Abstract      63 PARTHENOGENETIC DEVELOPMENT OF PORCINE OOCYTES RECONSTRUCTED BY CENTRIFUGATION AND ELECTROFUSION AFTER METAPHASE-II CHROMOSOME TRANSFER   N. Maedomari, K. Kikuchi, M. Fahrudin, M. Nakai, M. Ozawa, J. Noguchi, K. Kaneko, T. Nagai and N. Kashiwazaki pp. 149-149 Abstract      64 PRODUCTION OF TRANSGENIC CLONED PIGS BY MEANS OF SOMATIC CELL NUCLEAR TRANSFER USING KUSABIRA-ORANGE GENE-TRANSFECTED CELLS   H. Matsunari, M. Kurome, R. Tomii, S. Ueno, K. Hiruma, H. Saito, K. Hiyama, N. Nakayama, M. Onodera, N. Tada and H. Nagashima pp. 150-15 Abstract      65 NUCLEAR REPROGRAMMING OF PORCINE CELLS AND THEIR USE AS DONOR CELL FOR NUCLEAR TRANSFER AFTER TREATMENT IN XENOPUS EGG EXTRACTS   K. Miyamoto, M. Ohnuki, N. Minami, M. Yamada and H. Imai pp. 150-150 Abstract      66 SUCCESSFUL ESTABLISHMENT OF PLURIPOTENT ntES CELL LINES FROM AGED MICE  E. Mizutani, S. Kishigami, N. V. Thuan, H. Ohta, T. Hikichi, B. H. Thuy, S. Wakayama, Y. Sakaide and T. Wakayama pp. 151-151 Abstract      67 CELL TYPE AND CULTURE OVER TIME EFFECT DNA METHYLTRANSFERASE 1 EXPRESSION IN BOVINE DONOR FIBROBLAST CELLS   K. Moore, E. Wroclawska, J. M. Kramer and S. L. Goicoa pp. 151-151 Abstract      68 GROWTH, REPRODUCTION, AND LACTATION IN SOMATIC CELL-CLONED HOLSTEIN COWS AND THEIR PROGENY   K. Nagano, J. Todoroki, M. Imamura and C. Kubota pp. 151-151 Abstract      69 APPLICATION OF BOVINE AMNIOTIC CELLS FOR PRENATAL GENETIC DIAGNOSIS AND NUCLEAR TRANSFER  Y. Nagao, T. Watanabe, R. Furutani, Y. Kato, R. Takahashi, M. Hirabayashi, S. Ueda and T. Kurihara pp. 152-152 Abstract      70 FETAL FIBROBLAST CELLS PERMEABILIZED WITH STREPTOLYSIN O IMPROVE THE RATES OF FUSION AND IN VITRO DEVELOPMENT OF PORCINE RECONSTRUCTED EMBRYOS  K. Naruse, Y. M. Shin, Y. S. Quan, C. S. Park and D. I. Jin pp. 152-153 Full Text      71 THE DEVELOPMENT OF INTRA- AND INTER-SPECIES CLONED EMBRYOS DERIVED FROM RABBIT OOCYTES: THE EFFECT OF DONOR CELL SOURCES   P. Numchaisrika, A. Thongphakdee, R. Rungsiwiwut, K. Pruksananonda, P. Virutamasen and M. Techakumphu pp. 153-153 Abstract      72 LIMITED REPROGRAMMING OF SOMATIC CELL NUCLEI TRANSFERRED INTO MOUSE IMMATURE OOCYTES   C. Palmieri, H. Fulka, J. Fulka, Jr, P. Loi, G. Ptak and L. Della Salda pp. 153-154 Abstract      73 RE-CLONING BY SOMATIC CELL NUCLEAR TRANSFER FROM A CLONED KOREAN NATIVE GOAT   J. K. Park, S. Y. Jung, S. H. Park, A. N. Ha, J. I. Jin, S. H. Sohn, J. S. Lee, J. Y. Jung and H. S. Park pp. 154-154 Abstract      74 STUDY OF TRANSGENIC GOATS EXPRESSING HUMAN GRANULOCYTE–MACROPHAGE COLONY-STIMULATING FACTOR IN THE MAMMARY GLAND  H. S. Park, S. Y. Jung, S. H. Park, J. K. Park, J. S. Lee, T. S. Kim, S. P. Hong, K. M. Choi, J. G. Seol and K. W. Park pp. 154-155 Abstract      75 INHIBITION OF NK CELL CYTOTOXICITY IN CLONED MINI-PIGS EXPRESSING HUMAN LEUKOCYTE ANTIGEN-G1 (HLA-G1) GENE   K. W. Park, G. S. Han, K. M. Choi, S. P. Hong, J. Y. Yoo, E. J. Kim, S. H. Kim, T. S. Kim, S. Y. Park, K. S. Jun, K. N. Heo, D. I. Jin, C. S. Park and J. G. Seol pp. 155-155 Abstract      76 PREOVULATORY EMBRYO TRANSFER INCREASES SUCCESS OF PORCINE SOMATIC CLONING  B. Petersen, A. Lucas-Hahn, E. Lemme, N. Hornen, P. Hassel and H. Niemann pp. 155-156 Full Text      77 LOSS OF SAND CAT CLONED EMBRYOS DURING EARLY PREGNANCY MAY BE ASSOCIATED WITH ABERRANT Oct-4 EXPRESSION  C. E. Pope, M. C. Gómez, M. Truhe, M. López, C. Dumas, A. Cole, L. Lyons and B. L. Dresser pp. 156-156 Abstract      78 BLIND ENUCLEATION OF OOCYTES IS HIGHLY EFFICIENT IN ZONA-FREE BOVINE CLONING   M. I. Prokofiev, O. I. Stepanov, A. V. Komissarow, T. A. Antipova, M. V. Pinyugina and G. P. Malenko pp. 156-157 Abstract      79 EFFECTS OF EPIDERMAL GROWTH FACTOR, β-MERCAPTOETHANOL, GLUCOSE, OXYGEN CONCENTRATION, AND FIBROBLAST SUBCULTURE ON THE DEVELOPMENT OF PORCINE NT EMBRYOS   H. B. Seok, J. H. Quan and S. K. Kim pp. 157-157 Abstract      80 EFFECT OF MEDIA, SYNCHRONIZATION OF FIBROBLAST CELLS, CULTURE TIME, OXYGEN CONCENTRATION, AND ACTIVATION ON NUCLEAR-TRANSFERRED PORCINE OOCYTES   J. H. Quan, H. B. Seok and S. K. Kim pp. 157-158 Abstract      81 MOUSE CLONING BY USING A LASER-ASSISTED ZONA OPENING AND ELECTRO-FUSION TECHNIQUE   R. Rungsiwiwut, A. Thongphakdee, P. Numchaisrika, P. Virutamasen and M. Techakumphu pp. 158-158 Abstract      82 EFFECT OF DONOR CELL TRANSFECTION EVENTS ON EMBRYO AND FETAL SURVIVAL IN CLONING  D. F. Salamone, C. B. Santos, J. L. Barañao, L. Bussmann, J. Artuso, C. J. Munar, G. Berra, M. Criscuolo and C. Melo pp. 158-159 Full Text      83 IN VITRO DEVELOPMENTAL ABILITIES OF PORCINE CLONED EMBRYOS RECONSTITUTED WITH CELL NUCLEI OF GENETICALLY TRANSFORMED FETAL FIBROBLASTS CYTOMETRICALLY DIAGNOSED ON CELL CYCLE AND APOPTOSIS   M. Samiec, M. Skrzyszowska, M. Bochenek, D. Lipinski and R. Slomski pp. 159-159 Abstract      84 GENE EXPRESSION DURING BLASTOCYST-TO-ELONGATED STAGE IN BOVINE EMBRYOS DERIVED FROM SOMATIC CELL NUCLEAR TRANSFER   K. Sawai, S. Moriyasu, H. Hirayama, A. Minamihashi and S. Onoe pp. 159-160 Abstract      85 PREGNANCIES AND VIABILITY OF THE OFFSPRING AFTER TRANSFER OF HANDMADE CLONED PORCINE EMBRYOS   M. Schmidt, I. B. Bøgh, Y. Du, Y. Zhang, J. Li, S. Purup and G. Vajta pp. 160-160 Abstract      86 CREATION OF PORCINE TRANSGENIC NUCLEAR-TRANSFERRED EMBRYOS RECONSTRUCTED WITH EGFP-EXPRESSING ADULT DERMAL FIBROBLAST CELLS ANALYZED ON APOPTOSIS  M. Skrzyszowska, M. Samiec, Z. Smorag, D. Lipinski and R. Slomski pp. 160-161 Abstract      87 EVALUATION OF MITOCHONDRIAL FUNCTION IN SINGLE CLONED MINIATURE PIG EMBRYOS BY MEASURING OXYGEN CONSUMPTION   S. Sugimura, M. Yokoo, K.-I. Yamanaka, T. Wakai, H. Abe, T. Shoji, H. Sasada, J. Kobayashi, H. Abe and E. Sato pp. 161-161 Abstract      88 CELL CYCLE SYNCHRONIZATION OF FIBROBLASTS DERIVED FROM TRANSGENIC CLONED CATTLE EAR SKIN: EFFECTS OF SERUM STARVATION, ROSCOVITINE, AND CONTACT INHIBITION   X.-Z. Sun, S.-H. Wang, Y.-H. Zhang, Y.-P. Dai and N. Li pp. 161-162 Abstract      89 ZYGOTICALLY ACTIVATED GENES ARE SUPPRESSED IN MOUSE NUCLEAR-TRANSFERRED EMBRYOS  T. Suzuki, N. Minami and H. Imai pp. 162-162 Abstract      90 GERM-LINE TRANSMISSION OF DONOR MITOCHONDRIAL DNA IN NUCLEAR TRANSFER-DERIVED COWS   K. Takeda, K. Kaneyama, M. Tasai, S. Akagi, M. Yonai, N. Miyashita, A. Onishi, T. Tagami, K. Nirasawa and H. Hanada pp. 162-162 Abstract      91 PRODUCTION OF A CLONED BOER GOAT (CAPRA HIRCUS) BY SOMATIC CELL NUCLEAR TRANSFER   Y. Tao, W. Han, M. Zhang, J. Ding and X. Zhang pp. 163-163 Abstract      92 INTER-GENERIC MARBLED CAT AND FLAT-HEADED CAT CLONED BLASTOCYSTS GENERATED FROM DOMESTIC CAT AND RABBIT OOCYTES   A. Thongphakdee, P. Numchaisrika, P. Ratanakorn, S. Kamolnorranath, K. Chatdarong and M. Techakumphu pp. 163-163 Abstract      93 EFFECT OF SEASON ON QUALITY OF OOCYTES, RESULTS OF IN VITRO MATURATION, AND SOMATIC CELL NUCLEAR TRANSFER IN SWAMP BUFFALO   N. T. Uoc, F. de Rennis, N. H. Duc, L. C. Bui, N. V. Hanh, N. V. Linh, N. T. Thanh, Q. X. Huu, D. D. Long and B. X. Nguyen pp. 163-164 Abstract      94 PRODUCTION OF AN α1,3-GALACTOSYLTRANSFERASE GENE KNOCKOUT CALF USING A SINGLE TARGETING VECTOR AND REJUVENATED CELL LINES  M. Urakawa, Y. Sendai, T. Sawada, Y. Shinkai, A. Ideta, K. Kubota, H. Hoshi and Y. Aoyagi pp. 164-164 Abstract      95 OBSERVATION OF CHROMOSOME DECONDENSATION, HISTONE H3 MODIFICATION, AND HP1 PROTEIN IN MOUSE CLONED EMBRYOS FOLLOWING INHIBITION OF HISTONE DEACETYLATIONS AND CYCLIN-DEPENDENT KINASE   N. Van Thuan, B. Hong-Thuy, S. Wakayama, S. Kishigami, H. Ohta, T. Hikichi, E. Mizutani and T. Wakayama pp. 164-165 Abstract      96 TRICHOSTATIN A IMPROVED THE QUALITY OF RABBIT NUCLEAR TRANSFER EMBRYOS  J. Xu, L.-Y. Sung, J. Zhang, X. Tian, Y. E. Chen, X. Yang and F. Du pp. 165-165 Full Text      97 CLONED CALF PRODUCTION BY AGGREGATION OF SOMATIC CELL NUCLEAR-TRANSFERRED EMBRYOS  D. Yamaguchi, W. Suzuki, N. Adachi, S. Akagi, S. Watanabe and S. Takahashi pp. 166-166 Full Text      98 BLASTOCYST DEVELOPMENT RATE OF CLONED CAT EMBRYOS USING SERIAL CLONING   X. J. Yin, H. S. Lee, E. G. Choi, X. F. Yu, B. H. Choi, M. S. Piao, D. H. Oh, S. S. Lee and I. K. Kong pp. 166-166 Abstract      99 AMOUNT OF TELOMERIC DNA IN GROWING CLONED CATTLE, THEIR PROGENY, AND THEIR ORGANS   B. C. Yang, G. S. Im, Y. H. Kim, D. H. Kim, S. H. Bae, Y. G. Ko, H. H. Seong, S. H. Shon and B. S. Yang pp. 167-167 Abstract      100 EFFECT OF DIFFERENT POST-ACTIVATION TREATMENTS ON THE DEVELOPMENT OF SOMATIC CELL NUCLEAR TRANSFER PIG EMBRYOS   H. Y. Yong, K. Song and E. Lee pp. 167-167 Abstract      101 DEVELOPMENTAL ARREST AND CYTOSKELETAL ANOMALIES OF RAT EMBRYOS RECONSTRUCTED BY SOMATIC CELL NUCLEAR TRANSFER  J. G. Yoo, S.-P. Demers, L. Lian and L. C. Smith pp. 168-168 Abstract      102 RABBIT CLONING: HISTONE ACETYLATION STATUS OF DONOR CELLS AND CLONED EMBRYOS   V. Zakhartchenko, F. Yang, R. Hao and E. Wolf pp. 168-168 Abstract      103 BIRTH OF CLONED PIGLETS DERIVED FROM AN OPTIMIZED IN VITRO BLASTOCYST PRODUCTION SYSTEM BY TRICHOSTATIN A TREATMENT   Y.-H. Zhang, Y.-T. Du, K. Zhang, J. Li, P. M. Kragh, K. Villemoes, A. M. Pederson, M. H. Schmidt, I. B. Bøgh, S. Purup, L. Bolund, A. L. Jørgensen, H.-M. Yang, N. Li and G. Vajta pp. 168-168 Abstract      104 DEVELOPMENTAL POTENTIAL OF CLONED EMBRYOS FROM ADULT AND FETAL OVINE SOMATIC CELLS  H. M. Zhou and Y. Chen pp. 169-169 Abstract      105 ALTERING SEMEN EXTENDER AND GLYCEROL CONCENTRATION TO OPTIMIZE RESULTS AFTER CRYOPRESERVATION OF EQUINE SPERMATOZOA   M. Aceves, J. Scherzer, L. Ray, G. Heusner and R. A. Fayrer-Hosken pp. 170-170 Abstract      106 USING A BUOYANT DENSITY GRADIENT AND NILE RED STAINING TO EVALUATE THE LIPID CONTENT OF BOS TAURUS AND BOS INDICUS OOCYTES  C. B. Ballard, C. R. Looney, B. R. Lindsey, J. H. Pryor, J. W. Lynn, K. R. Bondioli and R. A. Godke pp. 170-171 Full Text      107 DEVELOPMENT OF VITRIFIED BOVINE SECONDARY AND PRIMORDIAL FOLLICLES IN SCID MICE   R. M. Bao, E. Yamasaka, M. Moniruzzaman, A. Hamawaki, M. Yoshikawa and T. Miyano pp. 171-171 Abstract      108 EFFECT OF CAFFEINE TREATMENT ON MPF AND MAPK ACTIVITY AND PARTHENOGENETIC ACTIVATION OF VITRIFIED MII OVINE OOCYTES   L. Bogliolo, F. Ariu, S. Succu, S. Fois, G. Leoni and S. Ledda pp. 171-172 Abstract      109 EFFECTS OF VITRIFICATION PROCEDURES ON SUBSEQUENT DEVELOPMENT AND ULTRASTRUCTURE OF IN VITRO-MATURED SWAMP BUFFALO (BUBALUS BUBALIS) OOCYTES   D. Boonkusol, T. Faisaikarm, A. Dinnyes and Y. Kitiyanant pp. 172-172 Abstract      110 COMPARISON OF ANDROMED®, BIOXCELL®, AND BOTU-BOV® EXTENDERS FOR CRYOPRESERVATION OF BULL SEXED SEMEN  M. Q. Braga, R. V. R. Franco, L. F. Rodrigues, G. Galeli, K. M. Oliveira, F. A. C. Reis, M. F. A. Nishikawa and E. P. Moura pp. 172-172 Full Text      111 SLOW FREEZING AND VITRIFICATION OF IN VITRO-MATURED DOMESTIC CAT OOCYTES  J. Braun, C. Otzdorff, T. Tsujioka and S. Hochi pp. 173-173 Full Text      112 VIABILITY AND APOPTOSIS OF BOVINE FIBROBLASTS FROZEN IN A CONVENTIONAL (–20°C) OR LOW-TEMPERATURE (–80°C) FREEZER  L. Chacón, M. C. Gómez, J. Jenkins, S. P. Leibo, G. Wirtu, B. L. Dresser and C. E. Pope pp. 173-174 Full Text      113 RETINOIC ACID DOES NOT PROTECT AGAINST THE SELECTIVE DAMAGE TO THE BOVINE INNER CELL MASS INFLICTED BY VITRIFICATION   C. Díez, A. Rodríguez, C. De Frutos, J. N. Caamaño, N. Facal and E. Gómez pp. 174-174 Abstract      114 CRYOTOP VITRIFICATION FOR IN VITRO-PRODUCED BUFFALO (BUBALUS BUBALIS) EMBRYOS   A. De Rosa, L. Attanasio, L. Boccia, G. Pellerano, G. Campanile and B. Gasparrini pp. 174-175 Abstract      115 RELATIONSHIP BETWEEN IN VIVO FERTILITY OF MANCHEGA EWES LAPAROSCOPICALLY INSEMINATED WITH FROZEN–THAWED RAM SEMEN AND HETEROLOGOUS CALF IN VITRO FERTILIZATION  O. Garcia-Alvarez, A. Maroto-Morales, M. D. Perez-Guzman and A. J. Soler pp. 175-175 Abstract      116 EFFECT OF INCUBATION OF BOVINE EPIDIDYMAL SPERMATOZOA IN SEMINAL PLASMA PRIOR TO CRYOPRESERVATION  C. A. Guerrero, J. A. Jenkins, J. W. Lynn, K. R. Bondioli and R. A. Godke pp. 175-176 Full Text      117 EFFECT OF STEPWISE DILUTION ON THE VIABILITY OF FROZEN–THAWED BOVINE OOCYTES MATURED IN VITRO   A. Hamawaki, S. Hamano, M. Yoshikawa and K. Matsukawa pp. 176-176 Abstract      118 CRYOPRESERVATION OF CONVENTIONAL AND SEX-SORTED BULL SPERM USING A DIRECTIONAL FREEZING METHOD   H. Hayakawa, T. Yamazaki, M. Oshi, M. Hoshino, O. Dochi and H. Koyama pp. 176-177 Abstract      119 OXIDATIVE STRESS DURING THE CRYOPRESERVATION OF BOAR SPERMATOZOA   M. Hernandez, J. M. Vazquez, E. A. Martinez and J. Roca pp. 177-177 Abstract      120 SUCCESSFUL PRODUCTION OF PIGLETS DERIVED FROM VITRIFIED MORULAE AND EARLY BLASTOCYSTS WITHOUT DIRECT EXPOSURE TO LIQUID NITROGEN   Y. Hirayama, M. Ohkubo, K. Misumi and K. Imai pp. 177-178 Abstract      121 CRYOPRESERVATION OF BOVINE BIOPSIED EMBRYOS UNDER A MAGNETIC FIELD   A. Ideta, K. Hayama, M. Urakawa, N. Ohwada and Y. Aoyagi pp. 178-178 Abstract      122 INTRACELLULAR ICE FORMATION IN VITRIFIED MOUSE MORULAE DURING WARMING  B. Jin, K. Kusanagi, M. Ueda, K. Edashige and M. Kasai pp. 178-179 Abstract      123 CRYOPROTECTANT EXPOSURE AFFECTS THE PROTEOME OF THE MOUSE METAPHASE II OOCYTE   M. Larman, M. Katz-Jaffe, C. Sheehan, W. Schoolcraft and D. Gardner pp. 179-179 Abstract      124 TAXOL™ COULD PROMOTE EMBRYO DEVELOPMENT OF BOVINE OOCYTES VITRIFIED BY OPS  R. Morató, D. Izquierdo, M. J. Palomo, B. Anguita, A. R. Jiménez-Macedo, J. L. Albarracín, M. T. Paramio and T. Mogas pp. 179-180 Full Text      125 IN VITRO-PRODUCED BOVINE EMBRYOS VITRIFIED IN GLASS CAPILLARIES USING TCM-199 OR DULBECCO'S PHOSPHATE-BUFFERED SALINE AS VITRIFICATION-WARMING MEDIA   N. Mucci, G. G. Kaiser, G. Rios, R. H. Alberio, L. B. Ferré and J. Aller pp. 180-180 Abstract      126 CRYOPRESERVATION OF PORCINE EMBRYOS DERIVED FROM IVM OOCYTES  N. Nakayama, K. Hiruma, M. Kurome, R. Tomii, S. Ueno, H. Saito, H. Matsunari and H. Nagashima pp. 180-181 Abstract      127 IN VITRO DEVELOPMENT OF IVF CRYOPRESERVED BOVINE EMBRYOS SUPPORTED BY TCM-199 OR SOFaa CULTURE MEDIUM   A. C. Nicacio, W. B. Feitosa, M. Rovegno, R. Simões, J. S. de A. Gonçalves, M. E. O. D'Á. Assumpção and J. A. Visintin pp. 181-181 Abstract      128 IMPROVED POST-THAW MOTILITY, VIABILITY, AND FERTILITY ARE ACHIEVED BY HYDROSTATIC PRESSURE-TREATED BULL SEMEN  C. Pribenszky, M. Molnár, A. Horváth, G. Kútvölgyi, A. Harnos, O. Szenci, J. Dengg and J. Lederer pp. 181-182 Abstract      129 IMPROVEMENT OF BOAR SPERM CRYOSURVIVAL BY THE OPTIMIZATION OF CRYOPRESERVATION CONDITIONS  J. Roca, M. Hernandez, T. Cremades, J. M. Vazquez and E. A. Martinez pp. 182-182 Abstract      130 EFFECTIVENESS OF BUTYLATED HYDROXYTOLUENE AS EGG YOLK SUBSTITUTE FOR CRYOPRESERVATION OF BOAR SPERMATOZOA  L. Rodriguez-Vilar, M. Hernandez, C. Lopez-Sanchez, J. M. Vazquez, E. A. Martinez and J. Roca pp. 182-183 Abstract      131 BOVINE OOCYTE VITRIFICATION IS IMPROVED BY INCREASED PYRUVATE CONCENTRATION AND THE INCLUSION OF HYALURONAN IN THE VITRIFICATION MEDIUM   C. B. Sheehan, M. G. Larman and D. K. Gardner pp. 183-183 Abstract      132 SUCCESSFUL EMBRYO TRANSFER OF IN VIVO-PRODUCED RED DEER (CERVUS ELAPHUS) EMBRYOS AFTER CRYOPRESERVATION BY SLOW FREEZING OR VITRIFICATION  J. P. Soler, G. G. Kaiser, N. Mucci, L. B. Ferre and R. H. Alberio pp. 183-184 Full Text      133 VITRIFICATION CAUSES DAMAGE OF THE ANTIOXIDANT DEFENSE SYSTEM IN IVM PORCINE OOCYTES   T. Somfai, M. Ozawa, J. Noguchi, H. Kaneko, K. Ohnuma, N. W. K. Karja, M. Farhudin, N. Maedomari, A. Dinnyes, T. Nagai and K. Kikuchi pp. 184-184 Abstract      134 CONCEPTION RATES OF IN VITRO-PRODUCED BOVINE EMBRYOS CRYOPRESERVED IN 6% GLYCEROL AND TRANSFERRED BY THE DIRECT METHOD   N. Takada, S. Hayasaka and K. Chiba pp. 184-185 Abstract      135 SHORT-TERM CRYOPRESERVATION OF VITRIFIED MOUSE MORULAE AT –79°C  Y. Takagi, M. Shimizu, M. Morimura, S. Yokomizo, K. Hara and M. Sakamoto pp. 185-185 Full Text      136 CLOSED SYSTEM OF GEL-LOADING-TIP VITRIFICATION FOR IN VITRO-PRODUCED BOVINE BLASTOCYSTS BEFORE AND AFTER BIOPSY  K. Tominaga, F. Iwaki, E. Yamaguchi, Y.-M. Dou, I. Kijihana and S. Hochi pp. 185-186 Full Text      137 EFFECT OF POST-IVF DEVELOPMENTAL KINETICS ON SURVIVAL AND QUALITY OF VITRIFIED–WARMED DOMESTIC CAT BLASTOCYSTS  T. Tsujioka, C. Otzdorff, J. Braun and S. Hochi pp. 186-186 Full Text      138 EFFECT OF SEMINAL PLASMA ON CHILLING AND FREEZING OF CANINE SPERMATOZOA   I. Yu, Y. J. Kim, I. S. Kim, S. P. Leibo and N. Songsasen pp. 187-187 Abstract      139 OXIDATIVE STRESS INDUCED CHANGES OF GLUTATHIONE CONCENTRATION IN IN VITRO-PRODUCED BOVINE BLASTOCYSTS   M. Aksoy, C. Wrenzycki, D. Herrmann and H. Niemann pp. 187-187 Abstract      140 LOCALIZATION OF PRIMORDIAL GERM CELLS IN DAY 21 BOVINE EMBRYOS  N. I. Alexopoulos, N. T. D'Cruz and P. Maddox-Hyttel pp. 188-188 Abstract      141 INDICATIONS FOR DNA DOUBLE-STRAND BREAK REPAIR IN EARLY BOVINE EMBRYOS  A. Brero, D. Koehler, T. Cremer, E. Wolf and V. Zakhartchenko pp. 188-188 Abstract      142 RELATIONSHIP BETWEEN APOPTOSIS IN BOAR SEMEN AND DNA FRAGMENTATION IN PORCINE EMBRYOS  M. Bryla, M. Trzcinska and Z. Smorag pp. 188-189 Full Text      143 DEVELOPMENTAL EFFICIENCY IMPROVEMENTS OF NUCLEAR TRANSFER EMBRYOS BY REPROGRAMMING SOMATIC CELLS USING TESTIS-DERIVED CELL EXTRACTS   H.-Y. Choi, C. Won, B.-W. Kim, Y.-J. Kang, G.-H. Kang, Y. Oh, E.-R. Lee, S. Roh and S.-G. Cho pp. 189-189 Abstract      144 Oct-4 EXPRESSION PATTERN IN THE EQUINE EMBRYO   Y. H. Choi, H. D. Harding, A. D. Obermiller and K. Hinrichs pp. 189-190 Abstract      145 THE METHYLATION PATTERNS OF POTENTIAL DIFFERENTIALLY METHYLATED REGIONS IN BOVINE Xist, Impact, NDN, AND H19 GENES  N. T. D'Cruz, K. J. Wilson and M. K. Holland pp. 190-190 Abstract      146 LECTIN DOLICHOS BIFLOURUS AGGLUTININ (DBA) IS A SPECIFIC MARKER for PRIMITIVE GERM CELLS IN NEONATAL PORCINE TESTIS  S. Goel, M. Sugimoto, S. Kume, M. Yamada, N. Minami and H. Imai pp. 190-190 Abstract      147 GENE KNOCKDOWN OF MATERNAL- AND EMBRYONIC-EXPRESSED GREEN FLUORESCENT PROTEIN IN MURINE EMBRYOS BY THE INJECTION OF A SHORT INTERFERING RNA (siRNA)  K. Iqbal, W. A. Kues, J. W. Carnwath and H. Niemann pp. 191-191 Full Text      148 EFFECT OF FOLLISTATIN TREATMENT POST-FERTILIZATION ON TIME TO FIRST CLEAVAGE, DEVELOPMENT TO THE BLASTOCYST STAGE, AND CELL ALLOCATION OF IN VITRO-PRODUCED BOVINE EMBRYOS   K. B. Lee, A. Bettegowda, J. J. Ireland and G. W. Smith pp. 191-192 Abstract      149 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED PROTEINS IN THE UMBILICAL CORDS OF TERM AND PRE-TERM (35–36 WEEKS) INFANTS USING PROTEOMICS  S. Y. Lee, S. K. Cho, D. N. Kwon, J. Y. Park and J. H. Kim pp. 192-192 Abstract      150 THE DISTRIBUTION PATTERN OF PRIMORDIAL GERM CELLS IN EARLY CHICK EMBRYOS   Y. Nakamura, Y. Yamamoto, F. Usui, T. Ono, K. Takeda, K. Nirasawa, H. Kagami and T. Tagami pp. 192-193 Abstract      151 LIPID COMPOSITION OF FAT DROPLETS OF IN VIVO- AND IN VITRO PRODUCED PORCINE EMBRYOS  M. Romek, B. Gajda, E. Krzysztofowicz and Z. Smorag pp. 193-193 Abstract      152 DIELECTROPHORETIC BEHAVIOR OF BOVINE OOCYTES AND ZYGOTES IN RELATION TO DEVELOPMENT AND TRANSCRIPTIONAL ABUNDANCE   D. Salilew-Wondim, F. Rings, M. Hoelker, D. Jennen, E. Tholen, M. A. Sirard, K. Schellander and D. Tesfaye pp. 193-194 Abstract      153 IMMUNOCYTOCHEMICAL STUDIES OF OCT-4, SOX-2, AND β-TUBULIN III EXPRESSION IN EARLY PORCINE EMBRYOS   K. Schauser, T. S. Sundkvist, M. Vejlsted and P. Maddox-Hyttel pp. 194-194 Abstract      154 UNDERNUTRITION DURING THE PERICONCEPTIONAL PERIOD IN EWES AFFECTS THE BLOOD PRESSURE AND WEIGHT OF THEIR NEONATAL LAMBS   N. A. Smith, P. Lonergan, P. Duffy, T. F. Crosby, P. Quinn, F. McAuliffe and A. C. O. Evans pp. 194-195 Abstract      155 LAPAROSCOPIC EVALUATION OF OVARIAN REACTION TO HORMONE STIMULATION IN CYNOMOLGUS MONKEYS (MACACA FASCICULARIS)   H. Tsuchiya, C. Iwatani, J. Yamasaki, J. N. Okahara, N. Okahara and R. Torii pp. 195-195 Abstract      156 DIFFERENTIAL REORGANIZATION OF HISTONE ACETYLATION THROUGH ATP-DEPENDENT REMODELING FACTOR DURING PRONUCLEAR FORMATION IN PORCINE ZYGOTIC CHROMATINS  G. Wee, D.-B. Koo, J.-S. Kim, B.-S. Song, J.-S. Park, X.-L. Jin, Y.-Y. Lee, Y.-M. Han and K.-K. Lee pp. 195-195 Abstract      157 DYNAMIC CHANGES IN LOCALIZATION OF HP1α DURING BOVINE EMBRYOGENESIS  K. J. Wilson, S. Prashadkumar, M. A. Cooney, A. J. French, D. A. Jans, P. J. Verma, M. K. Holland and N. T. D'Cruz pp. 196-196 Abstract      158 LEADING BLASTOMERE OF A 2-CELL-STAGE PORCINE PARTHENOGENETIC EMBRYO CONTRIBUTES TO TROPHECTODERM FIRST  C. Won, S. K. Park, Y.-J. Choi, H. Kang and S. Roh pp. 196-196 Abstract      159 LOCALIZATION OF CLAUDIN FAMILY PROTEINS IN PIG EMBRYOS DURING PRE-IMPLANTATION DEVELOPMENT  S. Xu, J. Lee, H. Harayama and M. Miyake pp. 197-197 Abstract      160 DETERMINATION OF THE INCIDENCE OF EMBRYONIC MORTALITY IN SYNCHRONIZED EWES DURING THE BREEDING SEASON   H. Erdem, M. K. Saribay and T. Tekeli pp. 197-197 Abstract      161 EFFECT OF MELOXICAM TREATMENT ON PREGNANCY RATES IN HOLSTEIN HEIFERS   A. Guzeloglu and H. Erdem pp. 197-198 Abstract      162 ESTRUS SYNCHRONIZATION, ARTIFICIAL INSEMINATION, AND PREGNANCY DIAGNOSIS IN WATER BUFFALOES (BUBALUS BUBALIS)  N. V. Hanh, Q. X. Huu, N. T. Uoc, B. X. Nguyen, J. Sulon, N. M. Sousa and J. F. Beckers pp. 198-198 Full Text      163 FUNCTIONAL COMPARISON OF BOVINE TROPHOBLASTIC VESICLES DERIVED FROM FRESHLY COLLECTED CONCEPTUSES AND SERIALLY PASSAGED TROPHOBLAST CELLS   Y. Hashiyada, H. Takahashi, M. Asada, N. Sakuta, S. Furuyashiki, S. Yamaya, T. Oike, K. Konishi and M. Takahashi pp. 198-199 Abstract      164 POTENTIAL REGULATION OF PERI-IMPLANTATION OVINE CONCEPTUS GROWTH AND DIFFERENTIATION BY THE WNT SIGNALING SYSTEM  K. Hayashi, R. C. Burghardt, F. W. Bazer and T. E. Spencer pp. 199-199 Abstract      165 EXPRESSION OF CALCIUM TRANSPORTER 1 GENE IN THE UTERUS OF RATS DURING PREGNANCY  B.-M. Lee, G.-S. Lee and E.-B. Jeung pp. 199-200 Abstract      166 DIFFERENTIAL EXPRESSION OF AMPHIREGULINE, EPIDERMAL GROWTH FACTOR, AND CALBINDIN-D9k IN THE UTERUS OF RODENTS AND PIGS DURING IMPLANTATION  G.-S. Lee, M.-H. Kim, S.-H. Hyun and E.-B. Jeung pp. 200-200 Abstract      167 EXPRESSION OF INTERFERON τ IN BOVINE EMBRYOS DERIVED FROM PARTHENOGENETIC ACTIVATION   A. Minamihashi, S. Moriyasu, H. Takahashi, H. Hirayama, M. Geshi, S. Onoe and K. Sawai pp. 200-200 Abstract      168 ESTABLISHMENT OF TROPHOBLAST STEM CELLS FROM MOUSE ANDROGENETIC AND PARTHENOGENETIC EMBRYOS  H. Ogawa, N. Shindo, S. Tanaka and T. Kono pp. 201-201 Abstract      169 PREDICTION OF THE OPTIMAL DAY OF MATING IN THE BITCH USING A BOVINE PLASMA PROGESTERONE ELISA KIT   M. Seki, Y. Yamazaki, N. Watanabe, K. Ishii, Y. Kinoshita, T. Aihara, S. Takeiri and T. Otoi pp. 201-201 Abstract      170 EXPRESSION PROFILE AND PROTEIN SECRETION OF BOVINE INTERFERON τ ON DAYS 12 TO 28 IN EARLY PREGNANCY  H. Takahashi, K. Sawai, A. Minamihashi, S. Kageyama, S. Moriyasu, H. Hirayama, S. Onoe and M. Geshi pp. 201-202 Abstract      171 CELLULAR EXPRESSION, LOCALIZATION, AND SECRETION OF OVINE Mx2 (oMx2) IN OVINE GLANDULAR EPITHELIAL CELLS (oGE)   K. Toyokawa, A. M. Assiri and T. L. Ott pp. 202-202 Abstract      172 DOES ESTRADIOL-17β CAUSE CYCLE-DEPENDENT MODULATIONS OF UTERINE MILK PROTEIN IN BOVINE ENDOMETRIUM?   S. E. Ulbrich, K. Gross, S. Schmidt, H. Blum, R. Rottmayer, S. Hiendleder, T. Fröhlich, G. J. Arnold, E. Wolf, H. H. D. Meyer and S. Bauersachs pp. 202-203 Abstract      173 HINDERING OF CLEAVAGE TIMING IN BOVINE PARTHENOTES DURING THE HOT SEASON   A. Aroyo, S. Yavin, Z. Roth and A. Arav pp. 203-203 Abstract      174 THE EFFECT OF DIFFERENT OVARY TRANSPORT TEMPERATURES ON IN VITRO DEVELOPMENT AND POST-THAW SURVIVAL OF BOVINE EMBRYOS   S. R. Cho, S. H. Choi, H. J. Kim, C. Y. Choe, H. J. Jin and D. S. Son pp. 203-204 Abstract      175 DETECTION OF LIPID GRANULES ON IVF BLASTOCYSTS MATURED IN APO-TRANSFERRIN IN KOREAN NATIVE COWS (HANWOO)   S. H. Choi, S. R. Cho, M. H. Han, H. J. Kim, C. Y. Choe, D. S. Son, J. S. Woo, B. D. Sang, Y. G. Chung and H. Hoshi pp. 204-204 Abstract      176 EFFECT OF LEUKEMIA INHIBITORY FACTOR FROM HUMAN AND MOUSE ORIGIN ON THE DEVELOPMENT OF BOVINE EMBRYOS PRODUCED IN VITRO   C. De Frutos, A. Rodríguez, C. Díez, J. N. Caamaño, N. Facal, P. Duque, C. García-Ochoa and E. Gómez pp. 204-205 Abstract      177 ISOLATION AND CULTURE OF MOUSE ENDOMETRIAL CELLS IN A THREE-DIMENSIONAL CULTURE SYSTEM  T.-Y. Fu, P.-C. Tang and J.-C. Ju pp. 205-205 Abstract      178 IN VITRO CULTURE OF BOVINE EMBRYOS WITH NEUROTROPHINS   E. Gómez, A. Rodríguez, C. De Frutos, J. N. Caamaño, N. Facal and C. Díez pp. 205-206 Abstract      179 EMBRYO DEVELOPMENT AND EXPERIMENT OUTCOME CAN BE COMPROMISED WITHOUT APPROPRIATE QUALITY CONTROL  D. K. Gardner, D. W. Linck and C. B. Sheehan pp. 206-206 Abstract      180 DEVELOPMENTAL CAPACITY OF SINGLE CULTIVATED EMBRYOS IN SMALL AND BIG DROPLETS  I. G. F. Goovaerts, J. B. P. De Clercq, M. Nichi and P. E. J. Bols pp. 206-207 Full Text      181 DIFFERENCES EXIST IN ZONA PELLUCIDA HARDNESS BETWEEN IN VIVO- AND IN VITRO-GENERATED BOVINE EMBRYOS   F. Itoi, T. Himaki, C. Kubota, J. Hirose, M. Miyamura, S. Hamano, Y. Murayama, S. Omata and M. Yoshida pp. 207-207 Abstract      182 INTERACTION OF MICROTUBULES AND MICROFILAMENTS WITH DISTRIBUTION OF MITOCHONDRIA IN TWO-CELL HAMSTER EMBRYOS   K. Kabashima, M. Matsuzaki and H. Suzuki pp. 207-208 Abstract      183 ADDITION OF DIPHENYLENEIODONIUM OR DEHYDROEPIANDROSTERONE TO CULTURE MEDIA INHIBITS REACTIVE OXYGEN SPECIES PRODUCTION DURING THE EARLY CLEAVAGE STAGES OF IN VITRO-PRODUCED PORCINE EMBRYOS   N. W. K. Karja, K. Kikuchi, M. Ozawa, M. Fahrudin, T. Somfai, M. Nakai, K. Ohnuma, J. Noguchi, H. Kaneko and T. Nagai pp. 208-208 Abstract      184 EFFECT OF CONCENTRATION AND EXPOSURE DURATION OF FETAL BOVINE SERUM ON PARTHENOGENETIC DEVELOPMENT OF PORCINE FOLLICULAR OOCYTES  H. J. Kim, S. R. Cho, C. Y. Choe, S. H. Choi, D. S. Son, S. J. Kim, Y. G. Kim, M. H. Han, I. S. Ryu, I. C. Kim, I. H. Kim and K. S. Im pp. 208-209 Full Text      185 PGI2 ANALOG INDUCES HIGH-QUALITY PORCINE BLASTOCYSTS IN VITRO   J.-S. Kim, G. Wee, B.-S. Song, J.-S. Park, X.-L. Jin, Y.-Y. Lee, Y.-M. Han, K.-K. Lee and D.-B. Koo pp. 209-209 Abstract      186 THE EFFECT OF MATRIGEL ON THE DEVELOPMENT OF IN VITRO-FERTILIZED PORCINE EMBRYOS   S.-W. Kim, M.-J. Lee, B.-C. Yang, G.-S. Im, H.-H. Seong, B.-S. Yang, H.-T. Cheong and D.-H. Kim pp. 209-210 Abstract      187 INCREASED NUMBER OF EXPANDED BOVINE BLASTOCYSTS IN SOF AND CR1 RELATIVE TO KSOM   D. M. Kohl, R. L. Monson, L. E. Enwall and J. J. Rutledge pp. 210-210 Abstract      188 THE IMPACT OF OIL SOURCE AND TYPE OF GLUTAMINE ON MEDIA EQUILIBRATION TIMES AND EMBRYO DEVELOPMENT   D. W. Linck and D. K. Gardner pp. 210-211 Abstract      189 EFFECT OF PROGESTERONE AND EPIDERMAL GROWTH FACTOR ON IN VITRO-PRODUCED EIGHT-CELL BOVINE EMBRYOS IN A SERUM-FREE CULTURE MEDIUM  B. Merlo, E. Iacono and G. Mari pp. 211-211 Full Text      190 INFLUENCE OF IN VITRO CULTURE OF BOVINE EMBRYOS ON THE STRUCTURE OF THE ZONA PELLUCIDA   E. Mertens, U. Besenfelder, M. Gilles, M. Hölker, F. Rings, V. Havlicek, K. Schellander and A. Herrler pp. 211-212 Abstract      191 ALLEVIATION OF THE TWO-CELL BLOCK OF ICR, ddY, AND AKR MOUSE EMBRYOS BY BOVINE SERUM ALBUMIN   K. Ono, R. Ohishi, H. Imai and M. Yamada pp. 212-212 Abstract      192 THE EFFECT OF ZWITTERONIC BUFFERS AND PBS ON GENE TRANSCRIPTION OF BOVINE IN VIVO- AND IN VITRO-DERIVED EMBRYOS   A. T. Palasz, P. Beltrán Breña, S. Pérez-Garnelo, S. Fuentes, J. De la Fuente1 and A. Gutiérrez-Adán pp. 212-213 Abstract      193 DIFFERENTIAL EFFECTS OF CULTURE ON APOPTOTIC GENE EXPRESSION IN THE PRE-IMPLANTATION CLONED EMBRYOS OF MINIATURE PIG   M. R. Park, I. S. Hwang, H. J. Moon, J. H. Shim, D. H. Kim, B. C. Yang, H. J. Cheong, Y. G. Ko, B. S. Yang and G. S. Im pp. 213-213 Abstract      194 ANTIOXIDATIVE EFFECT OF BROWN ALGAE PHLOROTANNINS ON REACTIVE OXYGEN SPECIES GENERATION AND EMBRYO DEVELOPMENT OF PORCINE PARTHENOGENETIC EMBRYOS UNDER OXIDATIVE AND HEAT-STRESSED CONDITIONS   M. Takahashi, K. Nagayama, M. Sakatani, S. Kobayashi, K. Morishita and M. Asakawa pp. 213-214 Abstract      195 SODIUM SELENITE INCREASES DEVELOPMENTAL ABILITY AND DECREASES APOPTOSIS IN PORCINE PARTHENOTES  S. J. Uhm, M. K. Gupta, J. H. Yang and H. T. Lee pp. 214-214 Full Text      196 A NEW APPROACH TO PREDICT MOUSE EMBRYONIC DEVELOPMENTAL COMPETENCE USING A TIME-LAPSE SYSTEM AND THE 'SHORTEST HALF' analysis procedure  S. Yavin, A. Aroyo, Z. Roth and A. Arav pp. 214-215 Full Text      197 EFFECTS OF HYALURONAN ON OXYGEN CONSUMPTION AND ATP CONTENT IN PIG BLASTOCYSTS PRODUCED IN VITRO   K. Yoshioka, M. Yokoo, T. Ozawa, C. Suzuki, H. Abe and H. Rodríguez-Martínez pp. 215-215 Abstract      198 BENEFICIAL EFFECT OF GHRELIN ON IN VITRO DEVELOPMENT OF PORCINE IN VITRO-FERTILIZED AND PARTHENOGENETIC EMBRYOS   K. Zhang, H. X. Wei, S. H. Wang, Y. H. Zhang, Y. Li, Y. P. Dai and N. Li pp. 215-216 Abstract      199 EVIDENCE FOR A NOVEL PERTURBATION IN CLONED FETUSES: MITOCHONDRIAL DNA DEPLETION   S. Hiendleder, D. Bebbere, S. E. Ulbrich, V. Zakhartchenko, M. Weppert, H.-D. Reichenbach, H. H. D. Meyer, S. Ledda and E. Wolf pp. 216-216 Abstract      200 EFFECT OF OOCYTE SIZE AND MICROMANIPULATION TECHNIQUES (ICSI AND EMBRYO BIOPSY) ON GOAT EMBRYO QUALITY  A. R. Jimenez-Macedo, B. Anguita, D. Izquierdo, R. Romaguera, R. Morato, T. Mogas and M. T. Paramio pp. 217-217 Full Text      201 FACTORS AFFECTING THE SURVIVAL OF BIOPSIED RHESUS MACAQUE EMBRYOS  H. M. Kubisch and M. S. Ratterree pp. 217-217 Abstract      202 INNER CELL MASS EXCHANGE IN SHEEP EMBRYOS  P. Loi, K. Matsukawa, C. Galli and G. Ptak pp. 218-218 Full Text      203 PRODUCTION OF ANDROGENETIC SHEEP BLASTOCYSTS  K. Matsukawa, M. Y. Turco, G. Ptak and P. Loi pp. 218-219 Full Text      204 RELATIONSHIP BETWEEN RESPIRATORY ACTIVITY AND THE PREGNANCY RATE OF BISECTED BOVINE EMBRYOS IN VIVO  S. Moriyasu, H. Hirayama, K. Sawai, S. Kageyama, S. Aoyagi, H. Shiku, T. Matsue, H. Abe, M. Kacchi, H. Hoshi and A. Minamihashi pp. 219-219 Abstract      205 INCREASED PREGNANCY RATES OF POOR QUALITY BOVINE EMBRYOS BY ASSISTED HATCHING   A. Taniyama, Y. Watanabe, Y. Nisino and T. Inoue pp. 219-220 Abstract      206 EFFECTS OF A COMBINATION OF A PRID, PGF2α AND eCG, ON ESTRUS SYNCHRONIZATION AND PREGNANCY RATE FOLLOWING EMBRYO TRANSFER IN HOLSTEIN HEIFERS   Y. Aoyagi, A. Ideta, M. Matsui, K. Hayama, M. Urakawa, A. Miyamoto and Y. Miyake pp. 220-220 Abstract      207 INFLUENCE OF THE INTERVAL BETWEEN THAWING TO TRANSFER ON PREGNANCY RATES OF FROZEN–THAWED DIRECT-TRANSFER EMBRYOS   G. A. Bo, L. C. Peres, D. Pincinato, M. de la Rey and R. Tribulo pp. 220-221 Abstract      208 EFFECT OF TWO CATTLE EMBRYO CO-CULTURE SYSTEMS ON CALVING RATE  A. M. Duszewska, J. Wojdan, W. Gawron, E. Wenta-Muchalska, B. Was, A. Wisniewska, A. Chromik and Z. Reklewski pp. 221-221 Full Text      209 VIABILITY OF PORCINE EMBRYOS VITRIFIED BY THE METAL MESH VITRIFICATION METHOD AFTER SURGICAL OR NONSURGICAL TRANSFER  Y. Fujino, Y. Nakamura, H. Kobayashi, S. Nakano, C. Suzuki and K. Yoshioka pp. 221-222 Abstract      210 FACTORS AFFECTING CONCEPTION RATE IN LACTATING HOLSTEIN RECIPIENTS   D. T. G. Jardina, C. A. Rodrigues and J. L. M. Vasconcelos pp. 222-222 Abstract      211 A COMPARISON OF PREGNANCY MAINTENANCE AFTER EMBRYO TRANSFER OF DAY 7 OR 13 BOVINE EMBRYOS AND THE RELATIONSHIP BETWEEN INTERFERON-TAU PRODUCTION OF DAY 13 BOVINE EMBRYOS AND PREGNANCY SUCCESS   K. Kimura, N. Hayashi and H. Iwata pp. 222-223 Abstract      212 EFFECT OF OVULATION STATUS ON PREGNANCY RATES IN RECIPIENT GILTS TRANSFERRED WITH CLONED EMBRYO TRANSFER   J. H. Lee, Y. W. Jeung, S. H. Hyun, E. S. Lee and E. B. Jeung pp. 223-223 Abstract      213 THE EFFECT OF eCG TREATMENT IN AN ESTRUS SYNCHRONIZATION PROTOCOL ON PREGNANCY RATES AFTER EMBRYO TRANSFER IN HOLSTEIN HEIFERS  T. Okazaki, E. Sasaki, K. Hasegawa, T. Takani and S. Abe pp. 223-224 Full Text      214 EFFECT OF TIMING OF PGF, eCG, AND ESTRADIOL BENZOATE IN RECIPIENTS SYNCHRONIZED WITH A PROGESTIN-BASED PROTOCOL FOR FIXED-TIME EMBRYO TRANSFER   L. C. Peres, D. Pincinato, L. Cutaia, R. Tribulo and G. A. Bo pp. 224-224 Abstract      215 COMPARISON BETWEEN CONVENTIONAL DIRECT TRANSFER FREEZING AND VITRIFICATION FOR THE CRYOPRESERVATION OF IN VIVO EMBRYOS FROM BRAHMAN CATTLE  J. H. Pryor, C. R. Looney, D. Walker, G. E. Seidel, Jr, J. F. Hasler, D. C. Kraemer and S. Romo pp. 224-225 Abstract      216 THE RELATIONSHIP BETWEEN OXYGEN CONSUMPTION RATE AND PREGNANCY RATE OF BOVINE EMBRYOS   N. Sakagami, K. Akiyama and Y. Nakazawa pp. 225-225 Abstract      217 A NEW APPROACH TO CRYOPRESERVATION OF LARGE EQUINE EMBRYOS BY VITRIFICATION AFTER BLASTOCOEL MICROMANIPULATION  J. Scherzer, R. A. Fayrer-Hosken, L. Ray and G. Heusner pp. 225-226 Abstract      218 BREEDING TRIAL OF CONGENITAL HYDROCEPHALUS OF JAPANESE BLACK CATTLE USING EMBRYO TRANSFER   Y. Shioya, T. Suzuki, Y. Sato, Y. Yoshikawa, Y. Hosokawa, K. Fukunari, Y. Sugimoto, T. Watanabe and N. Takenouchi pp. 226-226 Abstract      219 COMPARISON OF ESTRUS–OVULATION SYNCHRONIZATION TREATMENTS USING HOLSTEIN HEIFERS AS EMBRYO TRANSFER RECIPIENTS   H. Ushijima, N. Tatsuno, Y. Hara, T. Mori, H. Murata and M. Ohno pp. 226-227 Abstract      220 CpG METHYLATION PROFILE OF ADIPOGENIC AND ENDOTHELIAL GENE PROMOTERS IN HUMAN ADIPOSE STEM CELLS SUGGESTS A RESTRICTIVE ENDOTHELIAL DIFFERENTIATION POTENTIAL  A. C. Boquest, A. Noer, A. L. Sørensen, K. Vekterud and P. Collas pp. 227-227 Abstract      221 EPIGENETIC DYNAMICS OF PLURIPOTENCY GENES IN THE CONTEXT OF DIFFERENTIATION AND NUCLEAR REPROGRAMMING DETERMINED BY Q2ChIP, A QUICK AND QUANTITATIVE CHROMATIN IMMUNOPRECIPITATION TECHNIQUE APPLICABLE TO SMALL CELL SAMPLES   J. A. Dahl, C. K. Taranger and P. Collas pp. 227-228 Abstract      222 LOSS OF IMPRINTS OF PARTHENOGENETIC EMBRYONIC STEM CELLS IN MURINE CHIMERAS  T. Horii, M. Kimura, S. Morita, Y. Nagao and I. Hatada pp. 228-228 Abstract      223 COMPARISON OF NEURAL DIFFERENTIATION BETWEEN RAT AND CAT MESENCHYMAL STEM CELLS UNDER CHALLENGE BY THE SAME NEUROINDUCTION AGENT  G. Z. Jin, X. F. Yu, S. J. Cho, G. Y. Park, Y. J. Lee, X. J. Yin and I. K. Kong pp. 228-228 Abstract      224 DERIVATION AND IMMUNOLOGICAL CHARACTERISTICS OF PORCINE EMBRYONIC STEM-LIKE CELLS  J.-E. Kim, H.-Y. Son, H.-S. Kim, E.-G. Lee and C.-K. Lee pp. 229-229 Abstract      225 TERATOMA FORMATION BY BOVINE EMBRYOS  M. L. Lim, I. Vassiliev and P. J. Verma pp. 229-229 Abstract      226 CELL LINE-DEPENDENT GENE EXPRESSION PROFILES IN MOUSE EMBRYONIC STEM CELLS   S. Mamo, J. Kobolak, S. Becker, M. Horsch, J. Beckers and A. Dinnyes pp. 229-230 Abstract      227 DIFFERENTIATION OF HEPATOCYTE-LIKE CELLS FROM MOUSE EMBRYONIC STEM CELLS IN A MONOLAYER CULTURE SYSTEM   T. Mitani, T. Nagai, T. Masutani, H. Kato, K. Saeki, K. Matsumoto, Y. Hosoi and A. Iritani pp. 230-230 Abstract      228 ESTABLISHMENT OF EMBRYONIC STEM-LIKE CELL LINES IN RABBITS  Y.-W. Ou, K.-H. Lee, L.-R. Chen, P.-C. Tang, H.-F. Guu, M.-J. Chen and J.-C. Ju pp. 230-231 Abstract      229 DERIVATION OF PRESUMPTIVE GONOCYTES IN VITRO FROM PRIMATE EMBRYONIC STEM CELLS  T. Teramura, N. Kawata, T. Takehara, N. Fujinami, M. Takenoshita, K. Matsumoto, K. Saeki, N. Sagawa, Y. Hosoi and A. Iritani pp. 231-231 Abstract      230 GENERATION OF PUTATIVE RABBIT EMBRYONIC STEM CELLS  S. Wang, X. Tang, Y. Niu, H. Chen, T. Li, T. Tan and W. Ji pp. 231-232 Abstract      231 HISTOCOMPATIBLE PARTHENOGENETIC EMBRYONIC STEM CELLS  A. Yabuuchi, K. Kitai, A. Takeuchi, P. Lerou, K. Ng, J. West and G. Daley pp. 232-232 Abstract      232 SERUM-FREE DERIVATION OF CAT EMBRYONIC STEM-LIKE CELLS DERIVED FROM IN VIVO-PRODUCED BLASTOCYSTS ON CAT EMBRYONIC FIBROBLAST FEEDERS   X. F. Yu, X. J. Jin, D. S. Choi, D. K. Jung, B. H. Choi, S. G. Cho and I. K. Kong pp. 232-232 Abstract      233 NON-CROSS-CONTAMINATION OF BOVINE EMBRYOS WITH MICROBES USING THE OPS VITRIFICATION SYSTEM   A. Bielanski and A. Hanniman pp. 232-233 Abstract      234 QUANTITATION OF BOVINE VIRAL DIARRHEA VIRUS ASSOCIATED WITH SINGLE ZONA PELLUCIDA-INTACT IN VIVO-DERIVED BOVINE EMBRYOS  J. A. Gard, M. D. Givens, P. K. Galik, D. A. Stringfellow, K. P. Riddell, S. E. Marley and B. K. Whitlock pp. 233-233 Abstract      235 MORPHOMETRIC DETERMINATION OF ENDOMETRIAL LEUKOCYTE MIGRATION DURING DIFFERENT STAGES OF THE EQUINE ESTROUS CYCLE   D. Gerber and D. H. Volkmann pp. 233-234 Abstract      236 NORMAL REPRODUCTIVE CAPACITY OF HEIFERS RESULTING FROM TRANSFER OF IN VITRO-FERTILIZED EMBRYOS CULTURED WITH AN ANTIVIRAL COMPOUND  M. D. Givens, D. A. Stringfellow, K. P. Riddell and P. K. Galik pp. 234-234 Abstract      237 POTASSIUM CONCENTRATION AND mRNA LEVELS OF POTASSIUM CHANNELS DECREASED IN CYSTIC OVARIAN FOLLICLE FLUID   D. Kang, C. Choe, E. S. Kim, H. Y. Yang, C. G. Hur, E. J. Kim and J. Han pp. 234-234 Abstract      238 EFFICACY OF RECOMBINANT TRYPSIN AGAINST BOVINE HERPESVIRUS-1 ASSOCIATED WITH IN VITRO-DERIVED PORCINE EMBRYOS  M. S. D. Marley, M. D. Givens, P. K. Galik, K. P. Riddell and D. A. Stringfellow pp. 234-235 Full Text      239 EMBRYOTOXICITY ASSAY FOR ANTIVIRAL COMPOUND BPIP (5-[(4-BROMOPHENYL)METHYL]-2-PHENYL-5H-IMIDAZO[4,5-c]PYRIDINE) IN BOVINE IN VITRO-PRODUCED EMBRYOS  J. Mestach, J. Paeshuyse, J. Neyts, H. J. Nauwynck, D. Maes and A. Van Soom pp. 235-236 Full Text      240 RECOMBINANT BOVINE TRYPSIN MADE IN MAIZE INACTIVATES BOVINE HERPES VIRUS-1 ADSORBED TO THE BOVINE ZONA PELLUCIDA  G. E. Seidel, Jr, M. L. Turk, P. W. Gordy and R. A. Bowen pp. 236-236 Full Text      241 TIME POINTS FOR IN VITRO MATURATION OF LION (PANTHERA LEO) OOCYTES   S. Adamiak and P. Bartels pp. 236-237 Abstract      242 EFFECT OF SPERMATOZOA HEAD MORPHOMETRIC DIMENSIONS ON FREEZABILITY IN BROWN BEAR (URSUS ARCTOS)  L. Anel, V. Garcia-Macias, F. Martinez-Pastor, M. Alvarez, S. Borragan, J. Bernardo, S. Alves, C. Chamorro and P. Paz pp. 237-237 Abstract      243 IN VITRO PRODUCTION OF LLAMA EMBRYOS BY IVF OR ICSI  P. A. Conde, C. Herrera, M. G. Chaves, S. M. Giuliano, A. Director, V. L. Trasorras, M. Pinto, M. I. Sarchi, D. Stivale, B. Rutter, A. Agüero, M. H. Miragaya and R. S. Pasqualini pp. 237-238 Full Text      244 DEVELOPMENT OF SUPEROVULATORY STRATEGIES IN ALPACAS  G. Gamarra, A. Gallegos, M. Asparrin and H. W. Vivanco-Mackie pp. 238-238 Abstract      245 USE OF A TRIPLE STAIN (SYBR-14/PI/MC540) FOR VIABILITY AND CAPACITATION ASSESSMENT IN THAWED SEMEN FROM BROWN BEAR (URSUS ARCTOS)   V. Garcia-Macias, F. Martinez-Pastor, M. Alvarez, P. Paz, S. Borragan, E. Anel, M. Mata, M. Nicolas and L. Anel pp. 239-239 Abstract      246 IN VITRO PRODUCTION OF SPRINGBOK (ANTIDORCAS MARSUPIALIS) EMBRYOS   R. Krisher, A. Auer, K. Clark, K. Emsweller, S. Rogers, K. Thomas, F. Chatiza and P. Bartels pp. 239-240 Abstract      247 SUCCESSFUL ARTIFICIAL INSEMINATION IN THE CORN SNAKE (ELAPHE GUTATTA), USING FRESH AND COOLED SEMEN  J. K. Mattson, A. T. DeVries, S. M. McGuire, J. Krebs, E. E. Louis and N. M. Loskutoff pp. 240-240 Abstract      248 IN VITRO PRODUCTION OF CATTLE × BUFFALO HYBRID EMBRYOS USING BOVINE OOCYTES AND AFRICAN BUFFALO (SYNCERUS CAFFER CAFFER) EPIDIDYMAL SPERM  O. D. Owiny, D. M. Barry, M. Agaba and R. A. Godke pp. 240-240 Abstract      249 INTRACYTOPLASMIC SPERM INJECTION OF ELAND (TAUROTRAGUS ORYX) AND BONGO (TRAGELAPHUS EURYCERUS)ANTELOPE OOCYTES  G. Wirtu, C. E. Pope, M. C. Gomez, A. Cole, D. L. Paccamonti and B. L. Dresser pp. 241-241 Full Text      250 TRANSCRIPTIONAL PROFILING OF BOVINE OOCYTES DERIVED FROM THE GROWTH AND DOMINANCE PHASES OF THE FIRST FOLLICULAR WAVE  N. Ghanem, M. Hoelker, F. Rings, D. Jennen, E. Tholen, M. A. Sirard, K. Schellander and D. Tesfaye pp. 241-242 Abstract      251 FOLLICLE DEVIATION AND OVULATORY CAPACITY IN BOS INDICUS HEIFERS   L. U. Gimenes, N. A. T. Carvalho, M. F. Sá Filho, H. Ayres, J. R. S. Torres-Júnior, A. H. Souza, G. A. Bó, C. M. Barros, E. S. Sartorelli, P. S. Baruselli and R. J. Mapletoft pp. 242-242 Abstract      252 THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 (IGF-1) IN DEVELOPMENT OF AN ESTROGEN-ACTIVE DOMINANT FOLLICLE DURING THE FIRST FOLLICULAR WAVE POSTPARTUM IN DAIRY COWS   C. Kawashima, N. Sudo, C. Amaya Montoya, E. Kaneko, M. Matsui, N. Matsunaga, M. Tetsuka, T. Shimizu, K. Kida, Y.-I. Miyake and A. Miyamoto pp. 242-243 Abstract      253 GROWTH OF PRIMORDIAL OOCYTES FROM INFANT AND ADULT PORCINE OVARIES IN XENOGRAFTS   M. Moniruzzaman, J. Lee and T. Miyano pp. 243-243 Abstract      254 DEVELOPMENTAL COMPETENCE OF MOUSE OOCYTES AFTER IN VITRO GROWTH, NUCLEAR TRANSFER, AND IN VITRO FERTILIZATION   Y. Obata and T. Kono pp. 243-243 Abstract      255 EFFECT OF THE TYPE OF DIETARY FATTY ACID (α-LINOLENIC ACID OR LINOLEIC ACID) ON THE NUMBER AND THE QUALITY OF OOCYTES COLLECTED BY OVUM PICKUP AND ON IN VITRO EMBRYO PRODUCTION BY DAIRY HEIFERS   A. A. Ponter, J. Arnault, C. Joly, K. Guelou, G. Valy, S. Ponchon, C. Gonzales, B. Grimard and P. Humblot pp. 244-244 Abstract      256 SWITCH IN THE EXPRESSION OF GENES INVOLVED IN LIPID METABOLISM FOR IN VIVO-MATURED BOVINE OOCYTES AND BLASTOCYSTS  O. Algriany, P. L. A. M. Vos, M. A. Sirard and S. J. Dieleman pp. 244-244 Abstract      257 EXPRESSION PROFILING OF GENES CRUCIAL FOR LINEAGE DETERMINATION IN IN VITRO-DERIVED EARLY BOVINE EMBRYOS   S. Antonini, G. Lazzari, F. Cillo, C. Galli, S. Colleoni, I. Lagutina, F. Gandolfi and T. A. L. Brevini pp. 245-245 Abstract      258 EXPRESSION PATTERN OF THE MATERNAL FACTOR ZYGOTE ARREST 1 (ZAR1) GENE IN OVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS   F. Ariu, D. Bebbere, S. Succu, S. Tore, L. Bogliolo, S. Fois, G. G. Leoni and S. Ledda pp. 245-245 Abstract      259 DNA HYPOMETHYLATION OF FETAL FIBROBLASTS IMPROVES DEVELOPMENTAL COMPETENCY AND GENE EXPRESSION PATTERN IN PORCINE EMBRYOS FOLLOWING NUCLEAR TRANSFER   B. Mohana Kumar, H. F. Jin, J. G. Kim, S. A. Ock, H. J. Song, Y. J. Jeong, S. Balasubramanian and G. J. Rho pp. 246-246 Abstract      260 COMPARISON OF HOUSEKEEPING GENE EXPRESSION IN INDIVIDUAL 8-CELL STAGE MOUSE EMBRYOS PRODUCED UNDER DIFFERENT CONDITIONS  A. Baji Gal, S. Mamo, S. Bodo and A. Dinnyes pp. 246-246 Full Text      261 PHENOTYPIC CHARACTERISTICS AND TISSUE-SPECIFIC IGF2R/IGF2 EXPRESSION PARTITION BOVINE FETAL OVERGROWTH ASSOCIATED WITH IN VITRO FERTILIZATION AND SOMATIC CELL NUCLEAR TRANSFER CLONING   D. Bebbere, S. E. Ulbrich, V. Zakhartchenko, M. Weppert, H.-D. Reichenbach, H. H. D. Meyer, S. Ledda, E. Wolf and S. Hiendleder pp. 247-247 Abstract      262 ASSESSMENT OF HSP70-1 TRANSCRIPTION LEVELS IN IMMATURE OOCYTES FROM BOS TAURUS AND BOS INDICUS COWS RAISED IN A TROPICAL CLIMATE   L. S. A. Camargo, J. H. M Viana, R.V. Serapião, M. F. M. Guimarães, W. F. Sá and A. M. Ferreira pp. 247-248 Abstract      263 GENE EXPRESSION PROFILING OF IMMATURE AND IN VITRO-MATURED BOVINE OOCYTES USING AFFYMETRIX GENECHIP TECHNOLOGY   F. Carter, T. Fair, S. Park, M. Wade, A. C. O. Evans and P. Lonergan pp. 248-248 Abstract      264 ANALYSIS OF DNA METHYLATION PATTERN IN PRE-IMPLANTATION-STAGE EMBRYOS DERIVED FROM NUCLEAR TRANSFER USING PORCINE EMBRYONIC GERM CELLS  D.-H. Choi, C.-H. Park, S.-G. Lee, H.-S. Kim, H.-Y. Son and C.-K. Lee pp. 248-249 Full Text      265 DICER GENE EXPRESSION IN PRE-IMPLANTATION MOUSE EMBRYOS: IMPLICATION OF TRANSCRIPTION REGULATION AT THE BLASTOCYST STAGE   X. S. Cui, X. H. Shen, X. Y. Li, J. M. Kim and N. H. Kim pp. 249-249 Abstract      266 EFFECT OF 2,2′,4,4′-TETRABROMODIPHENYL ETHER ON THE EXPRESSION OF CALBINDIN-d9 k IN THE UTERUS OF IMMATURE RATS  V.-H. Dang and E.-B. Jeung pp. 249-249 Abstract      267 CLASSICAL AND NON-CLASSICAL MAJOR HISTOCOMPATABILITY COMPLEX CLASS I GENE EXPRESSION IN IN VITRO-DERIVED BOVINE EMBRYOS   J. Doyle, S. A. Ellis, P. Lonergan and T. Fair pp. 250-250 Abstract      268 THE EFFECTS OF METFORMIN ON THE INSULIN SIGNALING PATHWAY ON PORCINE GRANULOSA LUTEIN CELLS IN A PRIMARY CULTURE SYSTEM  M. S. Hossein, M. S. Lee and W. S. Hwang pp. 250-250 Abstract      269 EXPRESSION OF TANDEM-PORE DOMAIN K+ CHANNELS IN BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS   C. G. Hur, D. Kang, J. Y. Park, S. G. Hong and J. Han pp. 251-251 Abstract      270 IN VITRO MATURATION ALTERS THE EXPRESSION OF SPECIFIC PUTATIVELY IMPRINTED GENES IN BOVINE MII OOCYTES   M. G. Katz-Jaffe, B. R. McCallie, K. Preis, G. E. Seidel and D. K. Gardner pp. 251-251 Abstract      271 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN A 35-DAY-OLD CLONED PIG FETUS USING THE ANNEALING CONTROL PRIMER SYSTEM  Y. G. Ko, H. J. Chung, N. Y. Lee, H. J. Chung, H. J. Park, M. J. Kim, M. S. Choi, J. S. Kim, B. C. Yang, B. S. Yang, H. H. Seong and G. S. Im pp. 251-252 Abstract      272 EXPRESSION OF Lef1 TRANSCRIPTION FACTOR AND ITS NOVEL ISOFORMS IN PRE-IMPLANTATION EMBRYOS  A. Meece, S. He and C. Keefer pp. 252-252 Abstract      273 EXPRESSION PATTERN OF CONNEXIN 43 (Cx43) AND POLY-(A) POLYMERASE (PAP) GENES IN BUFFALO (BUBALUS BUBALIS) EMBRYOS PRODUCED IN VITRO  D. A. Mishra, V. Chandra, G. S. Kumar and G. T. Sharma pp. 252-252 Abstract      274 LOCALIZATION OF MACROPHAGE COLONY STIMULATING FACTOR (M-CSF) GENE IN THE ENDOMETRIUM OF JAPANESE BLACK COW   K. Oshima, K. Yoshihara, T. Kojima, M. Komatsu and N. Yamamoto pp. 253-253 Abstract      275 EXPRESSION OF THE ACYL-COA SYNTHETASE 4 IN THE PERI-IMPLANTATION MOUSE UTERUS  H. Y. Park, S. M. Lee, H.-M. Kim, S.-K. Yoon, S. J. Moon and M.-J. Kang pp. 253-253 Abstract      276 PRODUCTION OF TRANSGENIC MINI-PIG CELL LINES EXPRESSING HUMAN CYTOMEGALOVIRUS US2  K. W. Park, J. Y. Yoo, K. M. Choi, S. P. Hong, G. S. Han, E. J. Kim, S. H. Kim, T. S. Kim, S. Y. Park, B. S. Yang, G. S. Im, K. S. Jun, K. N. Heo and J. G. Seol pp. 254-254 Abstract      277 PRELIMINARY STUDY OF THE DIFFERENCE IN THE GENOME-WIDE METHYLATION PROFILES BETWEEN GERM CELLS AND SOMATIC CELLS OF BULLS  N. Phutikanit, J. Suwimonteerabutr, D. Harrison, M. J. D'Occhio, B. J. Carroll and M. Techakumphu pp. 254-254 Abstract      278 GENE EXPRESSION PROFILE OF OVINE OOCYTES AND CUMULUS CELLS WITH REFERENCE TO PREMATING NUTRITION   P. Pocar, L. F. Pisani, S. Antonini, V. Tosetti, T. A. L. Brevini, F. Gandolfi and S. M. Rhind pp. 254-255 Abstract      279 DIFFERENCES IN TRANSCRIPT ABUNDANCE BETWEEN MALE AND FEMALE BOVINE BLASTOCYSTS PRODUCED IN VITRO USING SEXED SEMEN  D. Rizos, P. Lonergan, D. Rath, J. De la Fuente, M. Wade, I. Parrilla-Riera and A. Gutierrez-Adan pp. 255-255 Abstract      280 EXPRESSION OF HSP70.1 GENE IN IN VITRO-PRODUCED BOVINE EMBRYOS CULTURED IN CR2 MEDIUM SUPPLEMENTED WITH KNOCKOUT™SR  R. V. Serapião, L. S. de Almeida Camargo, A. de Almeida Ramos, I. de Moura Folhadella, J. Polisseni, E. Lopes, J. H. Moreira Viana, A. de Moraes Ferreira, M. F. M. Guimarães, W. Ferreira de Sá and F. A. Fonseca pp. 255-256 Abstract      281 EXPRESSION PROFILE AND KNOCKDOWN ANALYSIS OF A FUNCTIONALLY UNKNOWN DD2-2 GENE IN MOUSE PRE-IMPLANTATION EMBRYOS   S. Shin, K. Matsumoto, T. Amano, K. Saeki, Y. Hosoi and A. Iritani pp. 256-256 Abstract      282 GENE EXPRESSION PATTERNS AND QUALITY OF BOVINE EMBRYOS PRODUCED UNDER DIFFERENT OXYGEN CONCENTRATIONS   W. J. Son, M. K. B. , Y. J. Jeong, S. Balasubramanian, S. Y. Choe and G. J. Rho pp. 256-257 Abstract      283 TRANSCRIPTIONAL ANALYSIS OF BIOPSIES DERIVED FROM IN VITRO-PRODUCED BOVINE BLASTOCYSTS IN RELATION TO PREGNANCY SUCCESS AFTER TRANSFER TO RECIPIENTS  D. Tesfaye, A. El-Sayed, M. Hoelker, F. Rings, D. Jennen, E. Tholen, M. A. Sirard and K. Schellander pp. 257-257 Abstract      284 IDENTIFICATION AND CHARACTERIZATION OF THE 5′-FLANKING REGION OF THREE MOUSE MATERNAL GENES (HISTONE H1OO, NUCLEOPLASMIN 2, AND ZYGOTE ARREST 1): TRANSCRIPTIONAL ACTIVITY IN MOUSE OOCYTES  K. Tsunemoto, K. Matsumoto, M. Anzai, M. Hayakumo, T. Amano, T. Mitani, H. Kato, Y. Hosoi, K. Saeki and A. Iritani pp. 257-258 Abstract      285 TRANSCRIPT LEVEL OF Hsp70 AND IGF2R GENES IN BOVINE INDIVIDUAL BLASTOCYSTS DERIVED FROM OOCYTES MATURED WITH FBS OR FATTY ACID-FREE BSA  E. Warzych, E. Pers, A. Buszka, T. Strabel and D. Lechniak pp. 258-258 Abstract      286 EFFECTS OF PORCINE FOLLICULAR FLUID DURING IN VITRO MATURATION OF OOCYTES ON IN VITRO FERTILIZATION AND EMBRYONIC DEVELOPMENT   B. Agung, T. Otoi, D. Fuchimoto, S. Senbon, A. Onishi and T. Nagai pp. 258-259 Abstract      287 RESCUE AND IN VITRO MATURATION OF FOLLICULAR OOCYTES IN CHINCHILLA LANIGER  G. Aiudi, M. Cinone, F. Maritato, A. De Sandro Salvati and M. E. Dell'Aquila pp. 259-259 Abstract      288 KINETICS OF FERTILIZATION, DEVELOPMENT, AND SEX RATIO OF IN VITRO-PRODUCED BOVINE EMBRYOS OBTAINED WITH FOUR DIFFERENT BULLS   M. Alomar, H. Tasiaux, S. Remacle, F. George, D. Paul and I. Donnay pp. 259-260 Abstract      289 EFFECT OF NEURAMINIDASE AND MANNOSIDASE IN THE BOVINE IN VITRO FERTILIZATION  S. Canovas, M. Aviles and P. Coy pp. 260-260 Abstract      290 ENZYMATIC ACTIVITY LEVEL OF SOME GLYCOSIDASES IN BOVINE OVIDUCTAL FLUID AT DIFFERENT STAGES OF THE ESTROUS CYCLE  C. Carrasco, R. Romar, J. Marcos, M. Aviles and P. Coy pp. 260-261 Full Text      291 EFFECTS OF ANTIOXIDANT AND CULTURE MEDIUM ON THE DEVELOPMENT OF PORCINE IN VITRO-MATURED–IN VITRO-FERTILIZED EMBRYOS   C. Choe, D.-S. Son, S.-H. Choi, S.-R. Cho, H.-J. Kim, S.-H. Yeon and M.-H. Han pp. 261-261 Abstract      292 OOCYTES FROM FOLLICLES OF DIFFERENT DIAMETERS: EMBRYO IN VITRO DEVELOPMENTAL POTENTIAL AND QUALITY   T. H. C. de Bem, K. R. L. Schwarz, L. G. Mesquita, P. R. Adona and C. L. V. Leal pp. 261-262 Abstract      293 EFFECT OF GUAIAZULENE ON IN VITRO BOVINE EMBRYO PRODUCTION   I. Dimitriadis, E. A. Rekka, E. Vainas, G. S. Amiridis and C. A. Rekkas pp. 262-262 Abstract      294 IN VITRO DEVELOPMENT OF RAT OOCYTES FOLLOWING MICROINJECTION OF A CUMULUS CELL INTO THE OOPLASM AND CHEMICAL ACTIVATION  W. Fujii and H. Funahashi pp. 262-262 Abstract      295 INFLUENCE OF FERTILIZATION MEDIUM ON THE INCIDENCE OF POLYSPERMY IN THE PIG  C. Galli, G. Crotti, P. Turini, I. Lagutina and G. Lazzari pp. 263-263 Full Text      296 EFFECT OF FROZEN SEMEN WITH OSTEOPONTIN ON IN VITRO BOVINE FERTILIZATION AND EMBRYO DEVELOPMENT   R. F. Gonçalves, R. P. Bertolla, I. Eder, D. A. Chapman and G. J. Killian pp. 263-264 Abstract      297 ADDITION OF GLUTATHIONE TO THAWING MEDIUM FOR BULL SPERMATOZOA IMPROVES THE IN VITRO EMBRYO PRODUCTION  L. Grullón, J. C. Gardó, F. García-Vázquez and J. Gadea pp. 264-264 Abstract      298 EFFECTS OF TRICHOSTATIN A DURING IN VITRO FERTILIZATION OF BOVINE OOCYTES ON SUBSEQUENT DEVELOPMENT, CELL NUMBER, AND ALLOCATION OF RESULTING EMBRYOS   S. Ikeda, K. Saeki, A. Tatemizo, D. Iwamoto, A. Kasamatsu, S. Taniguchi, Y. Hoshino, T. Amano, K. Matsumoto, Y. Hosoi and A. Iritani pp. 164-165 Abstract      299 LEPTIN ACCELERATES PRONUCLEAR FORMATION FOLLOWING INTRACYTOPLASMIC SPERM INJECTION OF PORCINE OOCYTES: POSSIBLE ROLE OF MITOGEN-ACTIVATED PROTEIN KINASE INACTIVATION   Y. X. Jin, X. S. Cui, W. K. Chang, T. Kim and N. H. Kim pp. 265-265 Abstract      300 BLASTOCYST PRODUCTION BY IN VITRO MATURATION AND DEVELOPMENT OF PORCINE OOCYTES IN DEFINED MEDIA FOLLOWING INTRACYTOPLASMIC SPERM INJECTION  Y. Kobayashi and M. Fukui pp. 265-265 Abstract      301 EFFECT OF CO-CULTURE WITH DIFFERENT STAGE EMBRYOS ON DEVELOPMENT OF ALGINATE-ENCAPSULATED SMALL NUMBER BOVINE EMBRYOS   S. Kobayashi, M. Sakatani, Y. Inaba, S. Kobayashi, K. Imai and M. Takahashi pp. 266-266 Abstract      302 EFFECT OF MONOSACCHARIDES ON SPERM ABILITY TO BIND TO OVIDUCTAL EPITHELIAL CELLS   Y. Kon, H. Iwata, H. Shiono, K. Matsubara, T. Kuwayama and Y. Monji pp. 266-266 Abstract      303 FERTILIZATION-RELATED PARAMETERS OF FROZEN–THAWED SPERMATOZOA FROM SUBFERTILE JAPANESE BLACK CATTLE   K. Kuroda, M. Fukushima, M. Miyake and H. Harayama pp. 266-267 Abstract      304 SHORT-PERIOD SPERM–OOCYTE INCUBATION AND FERTILIZATION MEDIA EFFECTS ON THE IN VITRO PRODUCTION OF SWINE EMBRYOS  M. G. Marques, A. B. Nascimento, L. F. Martins, F. R. O. Barros, M. D. Goissis, M. E. O. D'Avila Assumpção and J. A. Visintin pp. 267-267 Full Text      305 INCREASED MONOSPERMIC PENETRATION OF PORCINE OOCYTES USING A VISCOUS IN VITRO FERTILIZATION SYSTEM  H. Men, W. Si and J. K. Critser pp. 268-268 Full Text      306 IN VITRO PENETRATION OF SWINE OOCYTES MATURED IN TCM-199 WITH ADDED DIBUTYRYL CYCLIC ADENOSINE MONOPHOSPHATE AND FOLLICULAR FLUID   A. B. Nascimento, M. G. Marques, A. R. de S. Coutinho, M. N. Tavares, M. E. O. D'Avila Assumpção, J. A. Visintin, L. Che and V. Bordignon pp. 268-269 Abstract      307 ADDITION OF HIGH CONCENTRATIONS OF HEPARIN DURING IN VITRO FERTILIZATION IMPROVES PERCENTAGES OF CLEAVAGE AND BLASTOCYST FORMATION WHEN BOVINE OOCYTES ARE INSEMINATED IN VITRO WITH SEX-SORTED SPERM  T. L. Nedambale, J. Xu, S. A. Chaubal, X. C. Tian, X. Yang and F. Du pp. 269-269 Full Text      308 EFFECT OF GONADOTROPIN TREATMENT ON OOCYTE COLLECTION AND IN VITRO FERTILIZATION IN THE BAN MINIPIG   B. X. Nguyen, T. Nagai, K. Kukuchi, N. T. Uoc, M. Ozawa, N. V. Linh, N. H. Duc, D. N. Q. Thanh, N. V. Hanh and Q. X. Huu pp. 269-270 Abstract      309 DIFFERENCES IN PRONUCLEUS FORMATION RATES BETWEEN BULLS IN RESPONSE TO GAMMA IRRADIATION OF FROZEN–THAWED SEMEN   M. M. Petersen, G. B. Boe-Hansen, A. Birck, B. Avery, M. Jensen and I. B. Bøgh pp. 270-270 Abstract      310 DETERMINATION OF THE PORCINE OVIDUCTAL GLYCOSIDASES DURING THE ESTROUS CYCLE  R. Romar, C. Carrasco, J. Marcos, M. Avilés and P. Coy pp. 270-271 Full Text      311 EFFECT OF HEAT SHOCK DURING THE FERTILIZATION AND CULTURE PERIOD ON BOVINE EMBRYO DEVELOPMENT AND THE PROTECTIVE EFFECT OF BROWN ALGAE PHLOROTANNINS   M. Sakatani, K. Nagayama, K. Kobayashi, K. Kobayashi, K. Morishita, M. Asakawa and M. Takahashi pp. 271-271 Abstract      312 EXPOSURE OF SPERMATOZOA TO SOLUBILIZED EXTRACTS OF THE OVIDUCTAL EPITHELIUM APICAL PLASMA MEMBRANE ENHANCES FERTILIZATION IN PORCINE IN VITRO FERTILIZATION   N. Satake, A. K. Alhaider, W. V. Holt and P. F. Watson pp. 272-272 Abstract      313 IMPROVEMENT OF FERTILIZATION IN VITRO BY PRE-INCUBATION OF FROZEN–THAWED RAT SPERMATOZOA WITH CAFFEINE-SUPPLEMENTED MEDIUM   Y. Seita, S. Sugio, D. Sano, Y. Okuda and N. Kashiwazaki pp. 272-272 Abstract      314 OPTIMIZING TREATMENT FOR IVF WHEN USING HEAVILY BACTERIAL CONTAMINATED SEMEN   E. M. Senatore, R. Frontoso, D. Pirazzi, M. E. Mannino, G. Iovane and G. A. Presicce pp. 272-273 Abstract      315 SELECTION OF BOVINE OOCYTES BY BRILLIANT CRESYL BLUE BEFORE IN VITRO MATURATION IMPROVES BLASTOCYST DEVELOPMENT  A. Sugulle, S. Katakawa, S. Yamamoto, S. Oomori, I. Itou, O. Dochi and H. Koyama pp. 273-273 Full Text      316 DEVELOPMENTAL COMPETENCE OF OOCYTES SELECTED BY THE BRILLIANT CRESYL BLUE STAINING IN PREPUBERTAL AND ADULT CATTLE   M. Tagawa, S. Matoba, M. Okada, K. Metoki and K. Imai pp. 273-274 Abstract      317 ANTI-HYALURONIDASE ACTION OF ELLAGIC ACID EFFECTIVELY PREVENTS POLYSPERMY THROUGH SUPPRESSION OF THE ACROSOME REACTION INDUCED BY THE SPERM–ZONA INTERACTION DURING PORCINE IN VITRO FERTILIZATION   I. Tokeshi, H. Tatemoto, N. Muto, T. Yoshimoto, S. Nakamura and T. Nakada pp. 274-274 Abstract      318 EFFECT OF MATERNAL HEAT STRESS ON OOCYTE QUALITY AND IN VITRO COMPETENCE IN BOS INDICUS CATTLE   J. R. S. Torres Júnior, M. F. A. Pires, W. F. Sá, A. M. Ferreira, J. H. M. Viana, L. S. A. Camargo, A. A. Ramos, I. M. Folhadella, J. Polisseni, C. Freitas, C. A. A. Clemente, M. F. Sá Filho, C. M. Martins, G. A. Bó and P. S. Baruselli pp. 274-275 Abstract      319 PROTEIN GENE PRODUCT 9.5 REGULATES SPERM PENETRATION DURING PORCINE FERTILIZATION IN VITRO   Y. J. Yi, G. Manandhar, M. Sutovsky, C. S. Park and P. Sutovsky pp. 275-275 Abstract      320 EFFECT OF OXYGEN TENSION AND FOLLICLE CELLS DURING IN VITRO CULTURE OF PORCINE OOCYTES IN FOLLICULAR FLUID ON THEIR MATURATION AND FERTILIZATION  P. Yongnan, F. Daiichiro, S. Shoichiro, O. Akira, S. Eime and T. Nagai pp. 275-276 Abstract      321 PREGNANCIES ESTABLISHED FOLLOWING TRANSFER OF IN VITRO-PRODUCED FRESH AND FROZEN–THAWED BUFFALO (BUBALUS BUBALIS) EMBRYOS  X. Zhang, X. Liang, B. Yang, M. Chen, F. Huang, C. Pang, G. Qin, E. M. Senatore and G. A. Presicce pp. 276-276 Abstract      322 EFFECT OF SODIUM NITROPRUSSIDE ON BUFFALO SPERM CAPACITATION IN VITRO   L. Boccia, L. Attanasio, A. De Rosa, G. Pellerano, R. Di Palo and B. Gasparrini pp. 276-277 Abstract      323 EXPRESSION OF PSP-I AND PSP-II IN THE REPRODUCTIVE TRACT OF THE BOAR BY IMMUNOHISTOCHEMISTRY, WESTERN BLOTTING, AND RT-PCR  E. M. Garcia, J. M. Vazquez, I. Parrilla, J. J. Calvete, L. Sanz, E. A. Martinez, J. Roca and H. Rordríguez-Martínez pp. 277-277 Abstract      324 EFFECT OF AGING ON AMOUNTS OF DNA METHYLTRANSFERASE mRNA IN MOUSE SPERMATOZOA   H. Kato, T. Koda, M. Kishimoto, T. Mitani, K. Matsumoto, K. Saeki, Y. Hosoi and A. Iritani pp. 277-278 Abstract      325 PROLONGING THE LIFE OF RAM SPERMATOZOA IN VITRO USING OVIDUCTAL EPITHELIAL CELLS   R. E. Lloyd, E. Badia, P. F. Watson and W. V. Holt pp. 278-278 Abstract      326 COMPARING CHANGES IN MEMBRANE LIPID ORDER IN EPIDIDYMAL AND EJACULATED BOAR SPERMATOZOA  C. Matas, F. Garcia-Vazquez,, M. Sansegundo, S. Ruiz and J. Gadea pp. 277-278 Abstract      327 IDENTIFICATION OF OSTEOPONTIN IN WATER BUFFALO SEMEN  M. E. Pero, G. J. Killian, P. Lombardi, L. Zicarelli, L. Avallone and B. Gasparrini pp. 279-279 Abstract      328 FLUORESCENT PROBE ASSESSMENT OF POST-THAW QUALITY OF RAM SPERM TREATED WITH SEMINAL PLASMA  M. Rovegno, W. B. Feitosa, A. C. Brandão, A. B. Nascimento, M. A. Peres, J. A. Visintin and M. E. O. A. Assumpção pp. 279-280 Full Text      329 COMPARING CHANGES IN MOTION PARAMETERS IN EPIDIDYMAL AND EJACULATED BOAR SPERMATOZOA UNDER THREE DIFFERENT TREATMENTS   M. Sansegundo, J. C. Gardon, F. Garcia-Vazquez, J. Gadea and C. Matas pp. 280-280 Abstract      330 CORRELATION BETWEEN TWO APOPTOTIC MARKERS IN FRESH BOAR SPERMATOZOA   M. Trzcinska, M. Bryla and Z. Smorag pp. 280-281 Abstract      331 DIRECT EFFECTS OF ERGOT ALKALOIDS ON BOVINE SPERM MOTILITY IN VITRO   H. Wang, Z. Johnson, R. Rorie and C. Rosenkrans, Jr pp. 281-281 Abstract      332 EFFECT OF DIFFERENT PIG OOCYTE ACTIVATION PROTOCOLS ON EMBRYO DEVELOPMENT IN SOF AND NCSU-23  I. Lagutina, G. Lazzari and C. Galli pp. 281-282 Full Text      333 DYNAMICS OF MITOGEN-ACTIVATED PROTEIN KINASE ACTIVITY IN PIG OOCYTES FOLLOWING PARTHENOGENETIC ACTIVATION BY DIFFERENT METHODS   L. Nanassy, K. Lee, A. Javor and Z. Machaty pp. 282-282 Abstract      334 DNA SYNTHESIS DURING THE FIRST CELL CYCLE OF PORCINE OOCYTES FOLLOWING DIFFERENT ACTIVATION TREATMENTS   S.-A. Ock, D. Kang and J. Han pp. 282-283 Abstract      335 EFFECT OF VITAMIN E ON THE DEVELOPMENT OF BOVINE OOCYTES AFTER CHEMICAL ACTIVATION   J. I. Park, Y. Jang and E. S. Lee pp. 283-283 Abstract      336 DEVELOPMENT OF PORCINE NUCLEAR TRANSFER EMBRYOS PRODUCED USING SPERM CYTOSOLIC FACTOR (SCF) AT FUSION-ACTIVATION   J. H. Shim, I. S. Hwang, H. J. Moon, M. R. Park, D. H. Kim, B. C. Yang, Y. K. Ko, S. W. Kim, B. S. Yang, D. I. Jin and G. S. Im pp. 283-283 Abstract      337 GENE EXPRESSION IN BOVINE OOCYTES AFTER MEIOSIS BLOCK WITH BUTYROLACTONE I   P. R. Adona, F. H. Biase, T. H. C. de Bem and C. L. V. Leal pp. 284-284 Abstract      338 INFLUENCE OF OOCYTE DIAMETER AND MORPHOLOGY ON APOPTOSIS AND BLASTOCYST DEVELOPMENT IN PREPUBERTAL GOAT  B. Anguita, M. T. Paramio, A. R. Jimenez-Macedo, R. Romaguera, R. Morato, T. Mogas and D. Izquierdo pp. 284-285 Full Text      339 OXYGEN CONCENTRATION DURING IN VITRO MATURATION OF MURINE OOCYTES INFLUENCES SUBSEQUENT FETAL AND PLACENTAL OUTCOMES   K. M. Banwell, M. Lane, D. L. Russell, K. L. Kind and J. G. Thompson pp. 285-285 Abstract      340 EFFECT OF GLUCOSE ON THE MORPHOLOGY OF GERMINAL VESICLE AND MEIOTIC PROGRESSION OF PORCINE OOCYTES IN A CHEMICALLY DEFINED MEDIUM   H. Funahashi and T. Koike pp. 285-286 Abstract      341 IN VITRO MATURATION OF GOAT FOLLICULAR OOCYTES SUBSEQUENT TO THEIR CRYOPRESERVATION BY VITRIFICATION   N. Garg and G. N. Purohit pp. 286-286 Abstract      342 DISTINCT EFFECTS OF FOLLICULAR FLUID ON CUMULUS CELL APOPTOSIS AND PORCINE OOCYTE DEVELOPMENTAL COMPETENCE   C. G. Grupen, T. S. Hussein, S. J. Schulz and D. T. Armstrong pp. 286-287 Abstract      343 DISTRIBUTION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND IN VITRO MATURATION OF PORCINE OOCYTE–CUMULUS COMPLEXES FROM SMALL AND MEDIUM FOLLICLES   Y. Ishida and H. Funahashi pp. 287-287 Abstract      344 FOLLICULAR FLUIDS COLLECTED FROM LARGE FOLLICLES IMPROVED THE PROGRESSION OF IN VITRO NUCLEAR MATURATION OF PIG OOCYTES   M. Ito, M. Kitagawa, H. Iwata, T. Kuwayama and Y. Monji pp. 287-288 Abstract      345 EFFECT OF FOLLICLE STIMULATING HORMONE AND HUMAN CHORIONIC GONADOTROPHIN ON THE IN VITRO MATURATION OF CANINE OOCYTES   M.-K. Kim, H.-J. Oh, Y. H. Fibrianto, G. Jang, H.-J. Kim, S.-G. Hong, J.-E. Park, S.-K. Kang, B.-C. Lee and D.-Y. Kim pp. 288-288 Abstract      346 BOVINE EMBRYO DEVELOPMENT AFTER IVM/IVF/IVC OF OOCYTES STORED FOR 22 H IN VARIOUS MEDIA   C. Kubota, T. Kojima, T. Nagai, X. Tian and X. Yang pp. 288-288 Abstract      347 EFFECTS OF NEWBORN CANINE SERUM ON IN VITRO MATURATION OF CANINE OOCYTES   H. S. Lee, D. S. Choi, D. G. Jung, I. H. Bae, S. S. Lee, X. Y. Yin and I. K. Kong pp. 289-289 Abstract      348 INACTIVATION OF MAPK AFFECTS CENTROSOME ASSEMBLY, BUT NOT ACTIN FILAMENT ASSEMBLY, IN MOUSE OOCYTES MATURING IN VITRO   S. E. Lee, J. H. Kim, Y. K. Ji and N. H. Kim pp. 289-289 Abstract      349 DEVELOPMENTAL COMPETENCE AFTER IVF OF CUMULUS-ENCLOSED OOCYTES FROM A ICR MOUSE STRAIN CULTURED WITH A LOW CONCENTRATION OF HYPOXANTHINE DURING IVM   R. Oishi, K. Ono, M. Teranishi, H. Imai and M. Yamada pp. 289-290 Abstract      350 EFFECT OF INDIRECT MAINTENANCE OF INTRACELLULAR cAMP IN CUMULUS–OOCYTE COMPLEXES USING 3-ISOBUTYL-1-METHYLXANTHINE ON GAP JUNCTIONAL COMMUNICATIONS AND OOCYTE MEIOTIC MATURATION   M. Ozawa, T. Nagai, T. Somfai, M. Nakai, N. Maedomari, M. Fahrudin, N. W. K. Karja, H. Kaneko, J. Noguchi, K. Ohnuma and K. Kikuchi pp. 290-290 Abstract      351 EFFECTS OF FETAL CALF SERUM, GROWTH FACTOR, AND HORMONE SUPPLEMENTATION DURING IN VITRO MATURATION ON PARTHENOGENETIC ACTIVATION AND EMBRYO DEVELOPMENT OF FOLLICULAR RABBIT OOCYTES  Z. Polgar, T. Somfai, V. Angeli, X. H. Tang, W. Ji and A. Dinnyes pp. 290-291 Full Text      352 EFFECTS OF FOLLICLE SIZE AND STAGE OF MATURATION ON mRNA EXPRESSION IN BOVINE IN VITRO-MATURED OOCYTES   S. Racedo, D. Herrmann, C. Wrenzycki, D. Salamone and H. Niemann pp. 291-291 Abstract      353 EFFECTS OF CULTURE DURATION AND CUMULUS CELL REMOVAL ON CANINE OOCYTE IN VITRO MATURATION AND FERTILIZATION USING VASAL SPERM   M. Ridha-Albarzanchi, J. Liu, M. Kjelland and D. Kraemer pp. 292-292 Abstract      354 IN VITRO DEVELOPMENT OF BOVINE EMBRYOS AFTER NITRIC OXIDE INHIBITION   K. R. L. Schwarz, T. H. C. de Bem, T. T. Zampieri, P. R. Adona and C. L. V. Leal pp. 292-292 Abstract      355 DEVELOPMENTAL COMPETENCE OF IMMATURE BUBALINE CUMULUS–OOCYTE COMPLEXES VITRIFIED BY THE OPEN PULLED STRAW AND CONVENTIONAL STRAW METHODS   A. Sharma and G. N. Purohit pp. 293-293 Abstract      356 IMPROVED ENUCLEATION EFFICIENCY FOR PIG SOMATIC CELL NUCLEAR TRANSFER BY DENUDING OOCYTES AT 30 HOURS OF IN VITRO MATURATION   K. Song, J. Park and E. Lee pp. 293-294 Abstract      357 CALCIUM RELEASE INDUCED BY THIMEROSAL AND INOSITOL 1,4,5-TRIPHOSPHATE IN HEAT-SHOCKED PORCINE OOCYTES  J.-K. Tseng, P.-C. Tang and J.-C. Ju pp. 294-294 Abstract      358 MORPHOLOGICAL CHANGES IN VITRIFIED WATER BUFFALO CUMULUS–OOCYTE COMPLEXES AND THEIR IN VITRO NUCLEAR MATURATION  R. C. S. Yadav, A. Sharma and G. N. Purohit pp. 294-294 Abstract      359 THE EFFICIENT MAINTENANCE OF OXYGEN CONSUMPTION BY PORCINE OOCYTES RELATES TO THE HIGH COMPETENCE OF OOCYTE MATURATION  M. Yokoo, T. Ito-Sasaki, H. Shiku, T. Matsue, S. Aoyagi, H. Hoshi and H. Abe pp. 295-295 Abstract      360 EVALUATION OF QUALITY OF IN VITRO-MATURED BOVINE OOCYTES BY SEDIMENTATION WITH PERCOLL  K. Yotsushima, M. Shimizu, H. Kon and Y. Izaike pp. 295-296 Full Text      361 INFLUENCE OF SEXING PROCEDURE ON BULL SPERM CHROMATIN STRUCTURE  M. Bochenek, T. Herjan and Z. Smorag pp. 296-296 Abstract      362 SEX RATIO OF IN VITRO-PRODUCED BLASTOCYSTS OBTAINED USING FROZEN SEMEN FROM A CLONED BULL OR ITS ORIGINAL CELL DONOR   Y. Heyman, B. LeGuienne, C. Audouard, F. Charreaux, P. Humblot and X. Vignon pp. 296-297 Abstract      363 FACTORS INFLUENCING PREGNANCY RATES FOLLOWING TRANSFER OF BOVINE IN VIVO EMBRYOS BIOPSIED FOR SEX DETERMINATION   S. Li, W. Yu, J. Fu, Y. Bai, F. Jin and B. Shangguan pp. 297-297 Abstract      364 AMPLIFICATION AND APPLICATION OF THE HMG BOX OF BOVINE SRY GENE FOR SEX DETERMINATION  W. Lu, N. Rawlings and J. Zhao pp. 297-298 Abstract      365 COMPARISON OF IN VITRO FERTILIZING CAPACITY OF FROZEN–THAWED SEX-SORTED AND SEX-SORTED FROZEN–THAWED BULL SPERMATOZOA  V. Malcolm, M. Marfil, M. Calvi, F. Rigali, M. Pugliese, J. Gutierrez, M. Panarace and M. Medina pp. 298-298 Full Text      366 SEX IDENTIFICATION OF PORCINE EMBRYOS BY PCR BASED ON THE AMELOGENIN GENE  S. Senbon, S.-I. Suzuki, D.-I. Fuchimoto, M. Iwamoto, T. Kawarasaki and A. Onishi pp. 298-299 Abstract      367 FLUORESCENT IN SITU HYBRIDIZATION FOR SEX DETERMINATION AT PROGRESSIVE STAGES OF FERTILIZATION AND DEVELOPMENT IN MICE  J. J. Whyte, E. D. Fountain and C. S. Rosenfeld pp. 299-299 Abstract      368 FIELD STUDIES ON THE EFFECTIVENESS OF THE YCD EMBRYO SEXING TECHNIQUE IN BOVINE   W. Yu, S. Li, J. Fang, X. Sun, L. Cui, J. Fu, Y. Bai, Y. Fang and B. Shangguan pp. 299-300 Abstract      369 AMPLIFICATION OF THE SRY GENE FOR SEXING OF BUFFALO (BUBALUS BUBALIS) EMBRYOS USING NESTED MULTIPLEX PCR  M. Zhang, Q. Fu, W. S. Qin, H. Y. Zheng, Y. Q. Lu, S. S. Lu and K. H. Lu pp. 300-300 Abstract      370 BILATERAL DIFFERENTIAL TESTICULAR BIOPSIES IMPROVE TESE AND ICSI OUTCOMES IN NON-OBSTRUCTIVE AND OBSTRUCTIVE AZOOSPERMIC INFERTILE PATIENTS   S. M. Al-Ansari, M. T. Ridha-Albarzanchi, Z. A. Kanan, A. A. Al-Badri and S. K. Al-Atraqchi pp. 300-301 Abstract      371 INTRACYTOPLASMIC SPERM INJECTION OF EQUINE OOCYTES USING AIR-DRIED SPERM OR SPERM STORED IN A HIGH OSMOLARITY MEDIUM   A. Alonso, M. Miragaya, L. Losinno and C. Herrera pp. 301-301 Abstract      372 SPERM ASTER FORMATION AND BLASTOCYST DEVELOPMENT OF IN VIVO-MATURED BOVINE OOCYTES FERTILIZED BY INTRACYTOPLASMIC SPERM INJECTION   T. Horiuchi, M. Takenaka, C. Kani, C. Emuta, Y. Ogata and T. Matushige pp. 301-302 Abstract      373 OOCYTE-ACTIVATING CAPACITY OF BOVINE SPERMATOGENIC CELLS AND ACTIVATION PROTOCOLS FOR INTRACYTOPLASMIC INJECTION OF BOVINE ROUND SPERMATIDS   C. Kani, M. Takenaka, T. Muneto, M. Yamamoto and T. Horiuchi pp. 302-302 Abstract      374 THE EFFECTS OF POLYVINYLPYRROLIDONE ON BOVINE SPERM FUNCTION AND EMBRYO DEVELOPMENT   Y. Kato, M. Fukushima, A. Kenmotsu, K. Chikazawa and Y. Nagao pp. 302-303 Abstract      375 UNEXPECTED SEVERE ABNORMALITIES IN MOUSE ROSI OFFSPRING  P. N. Moreira, R. Fernández-González, M. Pérez-Crespo, P. Bermejo, J. D. Hourcade, R. Rey and A. Gutiérrez-Adán pp. 303-303 Full Text      376 EFFECT OF SPERM TREATMENT ON THE ABILITY TO ACTIVATE OOCYTES AFTER ICSI IN PIGS  M. Nakai, N. Kashiwazaki, N. Maedomari, M. Ozawa, J. Noguchi, H. Kaneko and K. Kikuchi pp. 303-304 Full Text      377 SPERMATOZOA RETRIEVED FROM MALE MICE FROZEN FOR 15 YEARS CAN PRODUCE NORMAL OFFSPRING  N. Ogonuki, K. Mochida, H. Miki, K. Inoue, T. Iwaki, K. Morozumi, R. Yanagimachi and A. Ogura pp. 304-304 Abstract      378 INTRACYTOPLASMIC SPERM INJECTION AND ESTABLISHMENT OF EMBRYONIC STEM CELLS IN AFRICAN GREEN MONKEYS (CERCOPITHECUS AETHIOPS)   N. Shimozawa, H. Okada, M. Hatori, T. Yoshida and T. Sankai pp. 304-305 Abstract      379 EFFECT OF SUPPLEMENTARY INOSITOL 1,4,5-TRIPHOSPHATE INJECTION ON FERTILIZATION AND EMBRYO DEVELOPMENT AFTER BOVINE ICSI   T. K. Suh and G.E. Seidel, Jr pp. 305-305 Abstract      380 FULL-TERM DEVELOPMENT OF MOUSE EMBRYOS PRODUCED FROM RECONSTRUCTED GIANT OOCYTES BY INTRACYTOPLASMIC SPERM HEAD INJECTION  S. Wakayama, S. Kishigami, V. T. Nguyen, H. Ohta, T. Hikichi, E. Mizutani, Y. Sakaide, B. H. Thuy, R. Suetsugu and T. Wakayama pp. 305-305 Abstract      381 FERTILIZABILITY AND CHROMOSOMAL INTEGRITY OF FROZEN–THAWED BRYDE's WHALE (BALAENOPTERA EDENI) SPERMATOZOA INTRACYTOPLASMICALLY INJECTED INTO MOUSE OOCYTES  H. Watanabe, H. Tateno, H. Kusakabe, Y. Kamiguchi, Y. Fujise, H. Ishikawa, S. Ohsumi and Y. Fukui pp. 306-306 Abstract      382 SYNCHRONIZING ACTIVATION TIME WITH MAXIMUM MATURATION PROMOTING FACTOR AND MITOGEN-ACTIVATED PROTEIN KINASE LEVELS DOES NOT IMPROVE BOVINE INTRACYTOPLASMIC SPERM INJECTION  B. J. Woodward, W. E. Maalouf and K. H. S. Campbell pp. 306-307 Full Text      383 EFFECT OF TIME OF OVULATION INDUCTION BY GONADOTROPIN-RELEASING HORMONE OR PITUITARY LUTEINIZING HORMONE ON OVA/EMBRYO PRODUCTION IN SUPERSTIMULATED BEEF COWS INSEMINATED AT A FIXED TIME  P. Chesta, R. Tribulo, H. Tribulo, E. Balla, P. S. Baruselli and G. A. Bo pp. 307-307 Full Text      384 EFFECTS OF CONDITION SCORE ON OVARIAN RESPONSE AND TRANSFERABLE EMBRYOS IN SUPERSTIMULATED COWS IN ARGENTINA  A. Garcia Guerra, D. Rodriguez, J. Villareal, A. Albrecht and G. Brogliatti pp. 307-308 Full Text      385 RELATIONSHIP AMONG ESTROUS BEHAVIOR, SUPEROVULATORY RESPONSE, AND EMBRYO SEX RATIO IN SUPERSTIMULATED HOLSTEIN HEIFERS   Y. G. Jung, A. Ideta, K. T. Lim, W. Y. Lee, H. B. Song, K. Hayama, M. Urakawa and Y. Aoyagi pp. 308-308 Abstract      386 EFFECTS OF TIME AND NUMBER OF INSEMINATIONS ON THE SEX RATIO OF EMBRYOS IN SUPERSTIMULATED HOLSTEIN HEIFERS  K. T. Lim, A. Ideta, W. Y. Lee, Y. G. Jung, K. Hayama, M. Urakawa and Y. Aoyagi pp. 308-309 Abstract      387 EFFECT OF NUMBER OF INSEMINATIONS ON EMBRYO PRODUCTION IN SUPERSTIMULATED NELORE (BOS INDICUS) COWS SUBJECTED TO FIXED-TIME ARTIFICIAL INSEMINATION   C. M. Martins, H. Ayres, J. R. S. Torres-Junior, A. H. Souza, G. A. Bó and P. S. Baruselli pp. 309-309 Abstract      388 SUPEROVULATION TREATMENT BY A SINGLE INJECTION OF FSH WITH ALUMINUM HYDROXIDE GEL IN HOLSTEIN CATTLE   S. Matoba, M. Tagawa, H. Matsuda, H. Yoshioka, K. Kimura, H. Kadokawa, M. Yonai and K. Imai pp. 309-310 Abstract      389 ASSOCIATION BETWEEN WALKING ACTIVITY AND SUPEROVULATORY RESPONSE IN JAPANESE BLACK CATTLE  M. Narita, M. Ito and Y. Tanimoto pp. 310-310 Full Text      390 THE EFFECT OF REDUCED FSH DOSE AND NUMBER OF TREATMENTS ON SUPEROVULATORY RESPONSE IN CIDR-TREATED KOREAN NATIVE COWS   D. S. Son, C. Y. Choe, S. R. Cho, S. H. Choi, H. J. Kim and I. H. Kim pp. 310-311 Abstract      391 EFFECTS OF SEASON AND LACTATION STATUS ON EMBRYO PRODUCTION IN HOLSTEIN COWS   J. L. M. Vasconcelos, D. T. G. Jardina, C. A. Rodrigues and V. C. Guerreiro pp. 311-311 Abstract      392 CHARACTERISTICS OF THE SUPEROVULATORY RESPONSE IN GYR (BOS INDICUS) CATTLE   J. H. M. Viana, L. S. A. Camargo, L. G. B. Siqueira, E. D. Souza, C. Freitas and C. A. C. Fernandes pp. 311-312 Abstract      393 A STUDY OF THE USE OF SEXED SEMEN IN SUPERSTIMULATED HOLSTEIN COWS  H. Wang, R. Q. Li, X. L. Zhang, Q. Z. Pan, J. L. Li, M. B. Zhao, Y. Z. Wang and D. Y. Chen pp. 312-312 Full Text      394 DEVELOPMENT OF A RAT ENDOMETRIAL SPHEROID AS A MODEL FOR THE ANALYSIS OF ENDOMETRIAL FUNCTIONS  K. Nishimura, S. Oozono, H. Urata, P. He, T. Soh, N. Yamauchi and M.-A. Hattori pp. 312-313 Abstract      395 ISOLATION AND CHARACTERIZATION OF PORCINE ADIPOSE-DERIVED ADULT STEM CELLS  A. A. Picou, S. L. Bartolotta, K. J. Williams, A. M. Giraldo, R. A. Godke and K. R. Bondioli pp. 313-313 Abstract      396 CHANGES OF PLASMINOGEN ACTIVATOR ACTIVITY IN OVIDUCTAL EPITHELIAL CELLS DURING THE ESTROUS CYCLE IN PIGS   M. Y. Shin, E. H. Kwon, H. T. Cheong, B. K. Yang and C. K. Park pp. 313-314 Abstract      397 LONG-TERM CULTURE OF BOVINE SECONDARY FOLLICLES IN MEDIA CONTAINING BSA, FCS, PLASMA, AND FOLLICULAR FLUID   A. Takajo, R. M. Bao, A. Hamawaki, M. Yoshikawa and T. Miyano pp. 314-314 Abstract      398 EFFECT OF INCUBATION PERIOD ON TRANSFECTION RATES IN BOVINE SPERMATOZOA  M. E. O. A. Assumpção, W. B. Feitosa, M. Rovegno, M. G. Marques, A. C. Nicacio, R. Simões and J. A. Visintin pp. 314-315 Full Text      399 SPERMATOZOA-MEDIATED GENE TRANSFER IS INFLUENCED BY SIZE, STRUCTURE, AND CONCENTRATION OF EXOGENOUS DNA  W. B. Feitosa, M. P. Milazzotto, E. A. L. Martins, A. M. Rocha, J. L. Avanzo, J. A. Visintin and M. E. O. A. Assumpção pp. 315-315 Full Text      400 PRODUCTION OF TRANSGENIC CLONED DOMESTIC CAT EMBRYOS BY LENTIVECTOR-MEDIATING TRANSGENESIS  M. C. Gómez, R. Kutner, D. Ricks, C. E. Pope, C. Dumas, B. L. Dresser and J. Reiser pp. 316-316 Abstract      401 USE OF FLOW CYTOMETRY TO EVALUATE THE CAPACITY OF BOAR SPERM TO BIND TO EXOGENOUS DNA OF DIFFERENT SIZES  F. García-Vázquez, D. Gumbao, A. Gutiérrez-Adan and J. Gadea pp. 316-316 Abstract      402 OOCYTE-MEDIATED GENE TRANSFER: A NOVEL APPROACH TO PRODUCE TRANSGENIC PORCINE EMBRYOS   M. K. Gupta, S. J. Uhm, E. Y. Kim, Y. H. Jung, J. Y. Yu and H. T. Lee pp. 317-317 Abstract      403 IDENTIFICATION AND CHARACTERIZATION OF A NOVEL MOUSE AND HUMAN MOPT GENE CONTAINING MORN MOTIF PROTEIN IN TESTIS  K. M. Kim, Y.-J. Choi, H. Song, K.-C. Hwang, S.-J. Kang, S.-K. Cho, M.-O. Kim and J.-H. Kim pp. 317-317 Abstract      404 DNA TAKE-UP BY SPERM AND IN VITRO EMBRYO DEVELOPMENT BY SPERM-MEDIATED GENE TRANSFER IN THE PIG   T. S. Kim, Y. Cao, H. T. Cheong, B. K. Yang and C. K. Park pp. 317-318 Abstract      405 COMPARISON OF TARGETING PARAMETERS FOR THE HPRT GENE BETWEEN DOMESTIC AND OSSABAW SWINE  K. Lee and Z. Machaty pp. 318-318 Abstract      406 EFFECTS OF SUCKLING INTENSITY ON MILK YIELD AND PIGLET GROWTH IN LACTATION-ENHANCED ALPHA-LACTALBUMIN TRANSGENIC GILTS   K. M. Marshall, W. L. Hurley, R. D. Shanks and M. B. Wheeler pp. 318-319 Abstract      407 AN ATTEMPT TO GENERATE TRANSGENIC CHICKENS BY INJECTION OF EGFP OR YFP GENE-TRANSFECTED BLASTODERMAL CELLS  Y. Matsubara, T. Tagami, H. Kagami and T. Nagai pp. 319-319 Abstract      408 FOREIGN GENE INTEGRATION PATTERNS IN TRANSGENIC PORCINE FETUSES PRODUCED BY ICSI-MEDIATED GENE TRANSFER   H. Saito, M. Kurome, R. Tomii, S. Ueno, K. Hiruma, K. Hiyama, H. Matsunari, N. Nakayama, K. Hattori, K. Nakamura, H. Endo, M. Watanabe, K. Umeyama, K. Miki and H. Nagashima pp. 319-320 Abstract      409 EFFICIENCY OF CO-TRANSFECTION OF TRANSGENE AND Neor GENE INTO GOAT AND PIG FETAL FIBROBLASTS  Y. M. Shin, S. M. Chang, B. C. Kim, C. S. Park and D. I. Jin pp. 320-320 Full Text      410 USE OF SPERMATOZOA AS VECTORS OF EXOGENOUS DNA FOR IN VITRO PRODUCTION OF BOVINE TRANSGENIC EMBRYOS  R. Simões, M. Binelli, A. C. Nicacio, M. P. Milazzotto, W. B. Feitosa, J. A. Visintin and M. E. O. A. Assumpção pp. 320-321 Abstract      411 EFFECTIVE PRODUCTION OF ICSI-MEDIATED TRANSGENIC MICE: INTENSITY OF GFP EXPRESSION AS A MARKER GENE AND INCIDENCE OF APOPTOSIS IN BLASTOCYSTS   M. Takenaka, C. Kani and T. Horiuchi pp. 321-321 Abstract      412 VIABILITY OF ENZYMATICALLY ISOLATED BOVINE PRIMORDIAL AND PRIMARY OVARIAN FOLLICLES COLLECTED BY THE BIOPSY PICK-UP TECHNIQUE  J. M. J. Aerts, B. Martinez-Madrid, K. Flothmann, J. B. P. De Clercq and P. E. J. Bols pp. 321-322 Abstract      413 ULTRASONOGRAPHIC EVALUATION OF FETAL LIVER SIZE DURING PREGNANCY IN BOVINE CLONED FETUSES  G. A. Jaúregui, M. Panarace, C. Garnil, A. Segovia, J. J. Lagioia, J. Gutiérrez, E. Rodriguez, M. Marfil and M. Medina pp. 322-322 Full Text      414 COLOR DOPPLER EVALUATION OF UMBILICAL BLOOD FLOW DURING PREGNANCY IN COWS  M. Panarace, G. Jaúregui, C. Garnil, A. Segovia, J. Gutiérrez, J. J. Lagioia, M. Marfil, E. Rodríguez and M. Medina pp. 323-323 Full Text      415 USE OF COMPUTER-ASSISTED ULTRASOUND IMAGE ANALYSIS IN EMBRYO RECIPIENT SELECTION   L. G. B. Siqueira, J . H. M. Viana, E. D. Souza, L. S. A. Camargo, J. F. Fonseca, C. A. C. Fernandes and C. A. A. Torres pp. 323-324 Abstract      416 EFFECTS OF THE CORPUS LUTEUM WITHIN THE OVARY ON THE FOLLICULAR DYNAMICS AFTER FOLLICULAR ASPIRATION AND ON THE DEVELOPMENTAL COMPETENCE OF ASPIRATED OOCYTES  T. Takuma, T. Otsubo, Y. Kurokawa and T. Otoi pp. 324-324 Abstract         Subscriber Login Username: Password:   Current Issue Volume 25 (4)  Submit Article Use the online submission system to send us your paper.  Advertisement

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