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RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.


 
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Reproduction, Fertility and Development Reproduction, Fertility and Development
Volume 19 Number 1 2007
Proceedings of the Annual Conference of the International Embryo Transfer Society, Kyoto, Japan, 6-10 January 2007
Full Papers and Abstracts for Poster Presentation


IETS 2007 author index 


pp. 325-334

 


IETS 2007 abstracts 


pp. 119-324

  
 


Recipient of the 2007 IETS Pioneer Award: Akira Iritani, PhD 


pp. xxxi-xxxi

 


Evaluation of oocyte quality: morphological, cellular and molecular predictors 

Qiang Wang and Qing-Yuan Sun

pp. 1-12

  
 


Oocyte cryopreservation: oocyte assessment and strategies for improving survival 

Sergio Ledda, Luisa Bogliolo, Sara Succu, Federica Ariu, Daniela Bebbere, Giovanni Giuseppe Leoni and Salvatore Naitana

pp. 13-23

  
 


Maternally derived transcripts: identification and characterisation during oocyte maturation and early cleavage 

Xiang-Shun Cui and Nam-Hyung Kim

pp. 25-34

  
 


Temporal and spatial control of gene expression in early embryos of farm animals 

Tiziana A. L. Brevini, Fabiana Cillo, Stefania Antonini, Valentina Tosetti and Fulvio Gandolfi

pp. 35-42

  
 


Embryo culture and long-term consequences 

Jeremy G. Thompson, Megan Mitchell and Karen L. Kind

pp. 43-52

  
 


Long-term effects of nutritional programming of the embryo and fetus: mechanisms and critical windows 

Michael E. Symonds, Terence Stephenson, David S. Gardner and Helen Budge

pp. 53-63

  
 


Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, interferons and endogenous retroviruses 

Thomas E. Spencer, Greg A. Johnson, Fuller W. Bazer, Robert C. Burghardt and Massimo Palmarini

pp. 65-78

  
 


Gene expression and maintenance of pregnancy in bovine: roles of trophoblastic binucleate cell-specific molecules 

Kazuyoshi Hashizume, Koichi Ushizawa, Osman V. Patel, Keiichiro Kizaki, Kei Imai, Osamu Yamada, Haruo Nakano and Toru Takahashi

pp. 79-90

  
 


State of the art in farm animal sperm evaluation 

H. Rodríguez-Martínez

pp. 91-101

  
 


Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction 

S. S. Suarez

pp. 103-110

  
 


Stem cells and lineage development in the mammalian blastocyst 

Janet Rossant

pp. 111-118

  
 


1 XENOGRAFTING OF ADULT MAMMALIAN TESTIS TISSUE  

L. Arregui, R. Rathi, W. Zeng, A. Honaramooz, M. Gomendio, E.R.S Roldan and I. Dobrinski

pp. 119-119

 


2 GLOBAL PEPTIDE SEQUENCING AND QUANTIFICATION OF PROTEINS IN PORCINE PARTHENOTES BY PROTEOMICS  

J. M. Jang, M. K. Gupta, J. W. Jung, S. J. Uhm, K. P. Kim and H. T. Lee

pp. 119-119

 


3 HISTONE H4 ACETYLATION AT LYSINE 12 AND Cdc2a EXPRESSION ARE DECREASED IN AGED MOUSE GERMINAL VESICLE-STAGE OOCYTES 

I. Manosalva, C. Goday and P. Esponda

pp. 120-120

 


4 MYOSTATIN GENE KNOCKDOWN THROUGH LENTIVIRAL VECTOR-MEDIATED DELIVERY OF shRNA FOR IN VITRO PRODUCTION OF TRANSGENIC BOVINE EMBRYOS 

M. P. Milazzotto, W. B. Feitosa, B. E. Strauss, M. Bajgelman, C. M. Mendes, M. E. O. A. Assumpção and J. A. Visintin

pp. 120-120

 


5 NUCLEOLAR DEVELOPMENT REQUIRES TRANSCRIPTIONAL ACTIVITY DURING PORCINE EMBRYONIC GENOME ACTIVATION 

O. Svarcova, P. Maddox-Hyttel, H. Niemann, D. Hermann, Z. Rasmussen, I. Petrovicova, F. Strejcek and J. Laurincik

pp. 120-121

 


6 TIMING OF DEADENYLATION OF GDF-9 AND CYCLIN 3′ UTR CONSTRUCTS IN BOVINE OOCYTES  

D. J. Walker, C. J. Wilusz and G. E. Seidel Jr

pp. 121-121

 


7 EFFECTS OF DIFFERENT TRYPSIN SOURCES AND CONCENTRATIONS ON THE VIABILITY OF BOVINE SPERM PRE- AND POST-CRYOPRESERVATION 

B. A. Blevins, S. Steenson and N. M. Loskutoff

pp. 121-122

 


8 PREGNANCY RATE FOLLOWING TIMED AI IN BEEF HEIFERS TREATED WITH CUE-MATE AND pLH OR GnRH 

M. G. Colazo, F. C. Dias, K. Lightfoot, O. Dochi, J. P. Kastelic and R. J. Mapletoft

pp. 122-122

 


9 CAN ONE PREDICT THE RESISTANCE OF BULL SPERM TO CRYOPRESERVATION BY ANALYZING MOTILITY PARAMETERS BEFORE FREEZING?  

L. Defoin, A. Granados and I. Donnay

pp. 123-123

 


10 EFFECTS OF INSEMINATION TIME, BREED, AND INSEMINATOR ON FERTILITY OF EWES INTRAUTERINALLY INSEMINATED WITH FROZEN–THAWED SEMEN IMPORTED FROM NEW ZEALAND  

Y. Fukui, H. Kohno, T. Togari, T. Matsuoka and H. Imai

pp. 123-123

 


11 FERTILITY OF FRESH AND FROZEN–THAWED EPIDIDYMAL STALLION SPERM WITH OR WITHOUT EXPOSURE TO SEMINAL PLASMA 

A. Heise, D. Gerber, D. H. Volkmann, W. Kähn and N. K. Brouwer

pp. 124-124

 


12 GENERATION OF RAT OFFSPRING DERIVED FROM CRYOPRESERVED SPERMATOZOA IN JAPANESE NATIONAL BIORESOURCES 

N. Kashiwazaki, Y. Seita, K. Naoi, A. Takizawa, T. Kuramoto and T. Serikawa

pp. 124-125

 


13 MANAGEMENT SYSTEM FOR FROZEN BULL SEMEN USING A RADIO FREQUENCY IDENTIFICATION TAG 

S. Kato, K. Mukojima, T. Otani, K. Sakai, K. Tamura, K. Miyazaki, N. Minami and H. Imai

pp. 125-125

 


14 MOTILITY AND FERTILITY OF BULL SPERMATOZOA FROZEN IN EGG YOLK EXTENDER SUPPLEMENTED WITH LACTOFERRIN  

J. Kobayashi, Y. Suda, N. Takada, K. Komine, T. Kuroishi, T. Kohsaka, H. Sasada and E. Sato

pp. 125-126

 


15 EFFECT OF BULL EPIDIDYMIS STORAGE CONDITIONS ON CRYOPRESERVED EPIDIDYMAL SPERM IN VITRO FERTILITY AND LIPID PEROXIDATION STATUS  

M. Nichi, T. Rijsselaere, A. Van Soom, J. B. P. De Clercq, I. G. F. Goovaerts, V. H. Barnabe and P. E. J. Bols

pp. 126-126

 


16 PROGESTERONE RELEASE PATTERNS FROM CUE-MATE IN COMPARISON TO OTHER INTRAVAGINAL PROGESTERONE-RELEASING DEVICES IN LACTATING DAIRY COWS  

D. Rogan, M. F. Martinez, G. A. Bo, P. Chesta, F. Feresin and R. J. Mapletoft

pp. 126-127

 


17 WHITETAIL DEER OFFSPRING FROM ARTIFICIAL INSEMINATION WITH EPIDIDYMAL SPERM HARVESTED FROM A HUNTER-KILLED BUCK  

J. R. Saenz, G. T. Gentry, W. Forbes, B. Olcott, J. Chenevert, K. R. Bondioli, D. E. Sanders and R. A. Godke

pp. 127-127

 


18 THE EFFECTS OF CIDR AND eCG TREATMENTS IN A GnRH-BASED PROTOCOL FOR TIMED AI OR EMBRYO TRANSFER ON PREGNANCY RATES IN LACTATING BEEF COWS  

J. A. Small, M. G. Colazo, J. P. Kastelic, D. R. Ward, O. Dochi and R. J. Mapletoft

pp. 127-128

 


19 EFFECTS OF ESTRADIOL OR GnRH ON FOLLICULAR WAVE EMERGENCE AND PREGNANCY RATES FOR A CIDR-BASED TIMED AI PROTOCOL IN REPEAT BREEDER DAIRY COWS  

G. H. Suh, U. H. Kim, T. Y. Hur, S. J. Kang, K. S. Baek, S. B. Park, H. S. Kim, H. G. Kang and I. H. Kim

pp. 128-128

 


20 SPERM CHARACTERIZATION OF ASTURCON PONIES AFTER COLLECTION AND EQUILIBRATION/REFRIGERATION BEFORE FREEZING  

C. Tamargo, C. Díez, J. De La Fuente, M. Carbajo, J. M. Benito and C. O. Hidalgo

pp. 128-129

 


21 RELATIONSHIP BETWEEN DURATION OF ESTRUS BEHAVIOR DETERMINED BY RADIOTELEMETORY SYSTEM AND THREE FACTORS (MILK PRODUCTION, STEROID HORMONE, AND OVULATION TIME) IN DAIRY COWS 

H. Yoshioka, H. Matsuda, K. Sasaguri and M. Yonai

pp. 129-129

 


22 RESPIRATORY ACTIVITY AND ULTRASTRUCTURAL FEATURES OF BOVINE SOMATIC NUCLEAR TRANSFER EMBRYOS 

H. Abe, K. Aoyagi, S. Aoyagi, H. Shiku, T. Matsue, Y. Sendai and H. Hoshi

pp. 129-130

 


23 HYPOMETHYLATION OF DNA IN NUCLEAR TRANSFER EMBRYOS FROM PORCINE EMBRYONIC GERM CELLS  

K. S. Ahn, S. Y. Heo, J. Y. Won and H. Shim

pp. 130-130

 


24 EFFECT OF TREATMENT WITH TRICHOSTATIN A ON IN VITRO DEVELOPMENT OF BOVINE NUCLEAR-TRANSFERRED EMBRYOS 

S. Akagi, K. Fukunari, K. Matsukawa, S. Watanabe and S. Takahashi

pp. 130-131

 


25 THE EFFECTS OF OOCYTE SOURCES AND ACTIVATION PROTOCOLS ON THE DEVELOPMENT OF CLONED GOAT EMBRYOS  

M. Apimeteeumrong, A. Thuangsanthia, N. Leingchaloen, V. Yiengvisavakul, A. Harintharanon, A. Kunavongkrit, X. Vignon and M. Techakumphu

pp. 131-131

 


26 SPERM REPRODUCTIVE POTENTIAL OF FETAL FIBROBLAST CELLS ON A NUCLEAR TRANSFER–CLONED BULL AND ON AN ORIGINAL PATERNAL BULL: A COMPARATIVE ANALYSIS  

S.-H. Bae, D.-H. Kim, S.-W. Kim, G.-S. Im, M.-J. Lee, J.-W. Ryu, H.-H. Seong, B.-S. Yang and B.-C. Yang

pp. 131-132

 


27 CYTOPLASM OF A GERMINAL VESICLE OOCYTE CAN PROMOTE SOMATIC NUCLEAR REPROGRAMMING IN MICE 

H.T. Bui, N. Van Thuan, S. Kishigami, S. Wakayama, T. Hikichi, H. Ohta, E. Mizutani and T. Wakayama

pp. 132-132

 


28 EXPRESSION OF MHC CLASS I IN THE PLACENTA AND THE LYMPHOCYTES OF BOVINE SOMATIC CLONES 

P. Chavatte-Palmer, M. Guillomot, J. Roïz, J.-L. Servely, Y. Heyman, I. Hue and S. Ellis

pp. 132-133

 


29 COMPARISON OF DNA METHYLATION RATES BETWEEN CLONED CATS AND DOMESTIC CATS 

S. J. Cho, S. H. Kang, S. G. Cho, I. H. Bae, C. J. Yang, X. J. Yin and I. K. Kong

pp. 133-133

 


30 GROWTH AND REPRODUCTIVE SUCCESS OF CLONED MALE CATS DERIVED FROM ADULT SOMATIC CELL NUCLEAR TRANSFER  

E. G. Choi, X. J. Yin, H. S. Lee, S. H. Kang, Y. J. Lee, L. H. Kim, H. D. Shin, N. H. Kim and I. K. Kong

pp. 133-134

 


31 EFFECT OF TREATMENT OF OVINE CYTOPLASTS WITH CAFFEINE ON THE TRANSLOCALIZATION OF HSP27 IN SOMATIC CELL NUCLEAR TRANSFER RECONSTRUCTED EMBRYOS 

I. Choi, J.-H. Lee and K. H. S. Campbell

pp. 134-134

 


32 EFFECTS OF FUSION/ACTIVATION METHODS ON DEVELOPMENT OF EMBRYOS PRODUCED BY NUCLEAR TRANSFER OF PORCINE FETAL FIBROBLASTS  

P. Q. Cong, E. S. Song, E. S. Kim, Z. H. Li, Y. J. Yi and C. S. Park

pp. 134-135

 


33 ENUCLEATION OF PRE-ACTIVATED MOUSE OOCYTES INDUCED BY DEMECOLCINE, NOCODAZOLE, AND VINBLASTINE 

N. Costa-Borges, J. Santaló and E. Ibàñez

pp. 135-135

 


34 PIGLETS BORN FROM HANDMADE CLONING  

Y. Du, Y. Zhang, J. Li, P. M. Kragh, M. Schmidt, I. B. Bøgh, X. Zhang, S. Purup, A. L. Jørgensen, A. M. Pedersen, K. Villemoes, H. Yang, L. Bolund and G. Vajta

pp. 135-136

 


35 RELATIONSHIP BETWEEN THE ONSET OF ENUCLEATION DURING MEIOTIC MATURATION OF RECIPIENT OOCYTES AND DEVELOPMENTAL ABILITY OF SOMATIC CELL NUCLEAR TRANSFER EMBRYOS IN PIGS  

M. Fahrudin, K. Kikuchi, N. W. K. Karja, M. Ozawa, T. Somfai, M. Nakai, K. Ohnuma, J. Noguchi, H. Kaneko and T. Nagai

pp. 136-136

 


36 EPIGENETIC MODIFICATIONS OF CULTURED BOVINE FIBROBLASTS FOR NUCLEAR TRANSFER  

A. M. Giraldo, J. W. Lynn, M. N. Purpera, R. A. Godke and K. R. Bondioli

pp. 136-137

 


37 ANATOMICAL DEVIATIONS IN APPARENTLY HEALTHY, WEANED, CLONED CALVES  

M. P. Green, M. C. Berg and R. S. F. Lee

pp. 137-137

 


38 RABBIT NUCLEAR TRANSFER AND IN VIVO-FERTILIZED EMBRYOS FAIL TO EXPRESS A MOUSE Oct-4 PROMOTER-DRIVEN EGFP REPORTER GENE 

R. Hao, A. Wuensch, R. Klose, E. Wolf and V. Zakhartchenko

pp. 137-138

 


39 BOVINE SOMATIC CELL NUCLEAR TRANSFER USING CUMULUS–OOCYTE COMPLEXES COLLECTED FROM THE IDENTICAL INDIVIDUAL BY OVUM PICKUP 

K. Hasegawa, S. Takahashi, S. Akagi, K. Takeda, K. Imai, M. Shimizu, T. Okazaki, S. Abe and Y. Izaike

pp. 138-138

 


40 TREATMENT OF DONOR CELLS AND ITS EFFECT ON INTERSPECIES NUCLEAR TRANSFER  

M. A. Hashem, D. P. Bhandari, S. K. Kang and B. C. Lee

pp. 138-139

 


41 TRANSMISSION OF A PARASITE-RESISTANT PHENOTYPE FROM A DONOR RAM TO ITS CLONED OFFSPRING  

J. Hill, W. Chang, R. Davey, K. Hutton and G. Riding

pp. 139-139

 


42 PREPARTUM HORMONAL CHANGES IN RECIPIENT COWS FOR SOMATIC CELL CLONED FETUSES  

H. Hirayama, K. Sawai, S. Moriyasu, S. Kageyama, S. Onoe, M. Hirayama, Y. Goto, E. Kaneko, A. Miyamoto and A. Minamihashi

pp. 139-140

 


43 INFLUENCE OF DONOR CELL DIFFERENTIATION ON CLONING EFFICIENCY IN PIGS  

N. Hornen, W. A. Kues, A. Lucas-Hahn, B. Petersen, P. Hassel and H. Niemann

pp. 140-141

 


44 NUCLEAR TRANSFER IN CATTLE USING SOMATIC CELLS FROM FROZEN TESTICLES WITHOUT CRYOPROTECTANTS 

Y. Hoshino, N. Kobayashi, N. Hayashi, T. Matsuhashi, K. Saeki, S. Ikeda, S. Taniguchi, A. Kasamatsu, D. Iwamoto, Y. Abe, K. Matsumoto, Y. Hosoi and A. Iritani

pp. 140-141

 


45 EFFECT OF OSMOLARITY IN CULTURE MEDIUM ON THE PRE-IMPLANTATION DEVELOPMENT OF PORCINE NT AND IVF EMBRYOS  

I. S. Hwang, H. J. Moon, J. H. Shim, M. R. Park, D. H. Kim, B. C. Yang, Y. G. Ko, B. S. Yang, H. T. Cheong and G. S. Im

pp. 141-141

 


46 EFFECT OF MANIPULATION MEDIUM ON THE DEVELOPMENT OF RECONSTRUCTED DOMESTIC CAT EMBRYOS 

S. Imsoonthornruksa, C. Lorthongpanich, K. Srirattana, N. Sripunya, C. Laowtammathron, M. Ketudat-Cairns and R. Parnpai

pp. 141-141

 


47 DIFFERENTIAL DEVELOPMENTAL ABILITY OF EMBRYOS CLONED FROM TISSUE-SPECIFIC STEM CELLS 

K. Inoue, N. Ogonuki, H. Miki, S. Noda, S. Inoue, K. Katayama, K. Mekada, H. Miyoshi and A. Ogura

pp. 142-142

 


48 EFFECTS OF TRICHOSTATIN A ON DEVELOPMENT OF BOVINE SOMATIC CELL NUCLEAR TRANSFER EMBRYOS  

D. Iwamoto, K. Saeki, S. Kishigami, A. Kasamatsu, A. Tatemizo, Y. Abe, S. Ikeda, S. Taniguchi, T. Mitani, H. Kato, K. Matsumoto, Y. Hosoi, T. Wakayama and A. Iritani

pp. 142-142

 


49 TRANSCRIPT LEVEL OF mRNA IN BOVINE CLONED OR RE-CLONED EMBRYOS 

G. Jang, H. Y. Jeon, K. H. Ko, H. J. Oh, H. J. Park, S. G. Hong, J. J. Kim, S. K. Kang and B. C. Lee

pp. 142-143

 


50 DNA METHYLATION PROFILES OF UPSTREAM ELEMENTS OF Oct-3/4 GENE IN IN VITRO FERTILIZATION (IVF) AND SOMATIC CELL NUCLEAR-TRANSFERRED (SCNT) EMBRYOS  

M. Kawasumi, Y. Unno, M. Nishiwaki, K. Matsumoto, M. Anzai, T. Amano, T. Mitani, H. Kato, K. Saeki, Y. Hosoi and A. Iritani

pp. 143-143

 


51 PROTEOMICS ANALYSIS OF PLACENTOMEGALY IN CLONED MICE 

H. R. Kim, K. Naruse, H. R. Lee, T. Wakayama, C. S. Park and D. I. Jin

pp. 143-144

 


52 EFFECT OF DONOR CELLS WITH VARIOUS DIFFERENTIATED STATUS ON THE DEVELOPMENTAL COMPETENCE OF PORCINE NUCLEAR TRANSFER EMBRYOS 

J. G. Kim, E. J. Kang, M. K. Kim, S. Y. Choe and G. J. Rho

pp. 144-144

 


53 EFFECTS OF INSULIN-TRANSFERRIN-SELENIUM IN DEFINED AND PORCINE FOLLICULAR FLUID-SUPPLEMENTED MEDIUM ON IN VITRO PRODUCTION OF PORCINE SCNT EMBRYOS  

Y. U. Kim, D. P. Bhandari, M. S. Hossein, S. M. Park, E. Lee, S. W. Park, Y. I. Jeong, J. Y. Lee, J. H. Kim, Y. W. Jeong, S. Kim and W. S. Hwang

pp. 144-145

 


54 SUCCESS OF MOUSE CLONING FROM AN OUTBRED STRAIN BY TRICHOSTATIN A TREATMENT AFTER SOMATIC NUCLEAR TRANSFER 

S. Kishigami, H. Bui, S. Wakayama, N. Van Thuan, T. Hikichi, E. Mizutani, H. Ohta, R. Suetsugu and T. Wakayama

pp. 145-145

 


55 DIFFERENTIAL TRANSCRIPTIONAL GENE EXPRESSION BETWEEN MINIATURE AND LANDRACE PIG FETUSES: IMPLICATION FOR PRODUCTION OF CLONED MINIATURE PIGS USING LANDRACE PIGS AS OOCYTE DONORS AND SURROGATE MOTHERS  

O. J. Koo, S. H. Lee, M. S. Hossein, S. K. Kang, D. Y. Kim and B. C. Lee

pp. 145-145

 


56 TRANSGENESIS BY HANDMADE CLONING USING EGFP-TRANSFECTED YUCATAN FIBROBLASTS 

P. M. Kragh, Y. Du, J. Li, Y. Zhang, L. Bolund and G. Vajta

pp. 146-146

 


57 NO BENEFICIAL EFFECT OF AGGREGATION ON INTERFERON TAU ACTIVITY IN BOVINE CLONE OUTGROWTHS 

S. Kurosaka, A. D. Ealy and K. J. McLaughlin

pp. 146-146

 


58 EFFECTS OF CAFFEINE AND VANADATE ON NUCLEAR REMODELING AND MICROTUBULE DISTRIBUTION OF PORCINE NUCLEAR TRANSFER EMBRYOS  

D. J. Kwon, C. K. Park, B. K. Yang, C. I. Kim and H. T. Cheong

pp. 146-147

 


59 HIGHER BLASTOCYST FORMATION OF SOMATIC CELL NUCLEAR TRANSFER EMBRYOS DOES NOT GUARANTEE BETTER PREGNANCY IN PIG 

E. Lee, K. Song, Y. Jeong and S. Hyun

pp. 147-147

 


60 IMPROVEMENT IN EFFICIENCY AND QUALITY OF CLONED AND PARTHENOGENETIC PORCINE EMBRYOS BY AMINO ACIDS  

H. T. Lee, J. M. Jang, S. H. Lee and M. K. Gupta

pp. 147-148

 


61 EFFICIENCY OF TWO ENUCLEATION METHODS FOR THE PRODUCTION OF TRANSGENIC PIG EMBRYOS BY HANDMADE CLONING 

J. Li, Y.-H. Zhang, Y.-T. Du, P. M. Kragh, S. Purup, A. M. Pederson, K. Villemoes, A. L. Jørgensen, L. Bolund, Q.-Z. Xue, H.-M. Yang and G. Vajta

pp. 148-148

 


62 EXPRESSION AND DISTRIBUTION OF Oct-4 IN INTERSPECIES-CLONED LONG-TAILED MONKEY (MACACA FASCICULARIS) EMBRYOS 

C. Lorthongpanich, K. Srirattana, S. Imsoonthornruksa, N. Sripunya, C. Laowtammathron, O. Kumpong, M. Ketudat-Cairns and R. Parnpai

pp. 149-149

 


63 PARTHENOGENETIC DEVELOPMENT OF PORCINE OOCYTES RECONSTRUCTED BY CENTRIFUGATION AND ELECTROFUSION AFTER METAPHASE-II CHROMOSOME TRANSFER  

N. Maedomari, K. Kikuchi, M. Fahrudin, M. Nakai, M. Ozawa, J. Noguchi, K. Kaneko, T. Nagai and N. Kashiwazaki

pp. 149-149

 


64 PRODUCTION OF TRANSGENIC CLONED PIGS BY MEANS OF SOMATIC CELL NUCLEAR TRANSFER USING KUSABIRA-ORANGE GENE-TRANSFECTED CELLS  

H. Matsunari, M. Kurome, R. Tomii, S. Ueno, K. Hiruma, H. Saito, K. Hiyama, N. Nakayama, M. Onodera, N. Tada and H. Nagashima

pp. 150-15

 


65 NUCLEAR REPROGRAMMING OF PORCINE CELLS AND THEIR USE AS DONOR CELL FOR NUCLEAR TRANSFER AFTER TREATMENT IN XENOPUS EGG EXTRACTS  

K. Miyamoto, M. Ohnuki, N. Minami, M. Yamada and H. Imai

pp. 150-150

 


66 SUCCESSFUL ESTABLISHMENT OF PLURIPOTENT ntES CELL LINES FROM AGED MICE 

E. Mizutani, S. Kishigami, N. V. Thuan, H. Ohta, T. Hikichi, B. H. Thuy, S. Wakayama, Y. Sakaide and T. Wakayama

pp. 151-151

 


67 CELL TYPE AND CULTURE OVER TIME EFFECT DNA METHYLTRANSFERASE 1 EXPRESSION IN BOVINE DONOR FIBROBLAST CELLS  

K. Moore, E. Wroclawska, J. M. Kramer and S. L. Goicoa

pp. 151-151

 


68 GROWTH, REPRODUCTION, AND LACTATION IN SOMATIC CELL-CLONED HOLSTEIN COWS AND THEIR PROGENY  

K. Nagano, J. Todoroki, M. Imamura and C. Kubota

pp. 151-151

 


69 APPLICATION OF BOVINE AMNIOTIC CELLS FOR PRENATAL GENETIC DIAGNOSIS AND NUCLEAR TRANSFER 

Y. Nagao, T. Watanabe, R. Furutani, Y. Kato, R. Takahashi, M. Hirabayashi, S. Ueda and T. Kurihara

pp. 152-152

 


70 FETAL FIBROBLAST CELLS PERMEABILIZED WITH STREPTOLYSIN O IMPROVE THE RATES OF FUSION AND IN VITRO DEVELOPMENT OF PORCINE RECONSTRUCTED EMBRYOS 

K. Naruse, Y. M. Shin, Y. S. Quan, C. S. Park and D. I. Jin

pp. 152-153

 


71 THE DEVELOPMENT OF INTRA- AND INTER-SPECIES CLONED EMBRYOS DERIVED FROM RABBIT OOCYTES: THE EFFECT OF DONOR CELL SOURCES  

P. Numchaisrika, A. Thongphakdee, R. Rungsiwiwut, K. Pruksananonda, P. Virutamasen and M. Techakumphu

pp. 153-153

 


72 LIMITED REPROGRAMMING OF SOMATIC CELL NUCLEI TRANSFERRED INTO MOUSE IMMATURE OOCYTES  

C. Palmieri, H. Fulka, J. Fulka, Jr, P. Loi, G. Ptak and L. Della Salda

pp. 153-154

 


73 RE-CLONING BY SOMATIC CELL NUCLEAR TRANSFER FROM A CLONED KOREAN NATIVE GOAT  

J. K. Park, S. Y. Jung, S. H. Park, A. N. Ha, J. I. Jin, S. H. Sohn, J. S. Lee, J. Y. Jung and H. S. Park

pp. 154-154

 


74 STUDY OF TRANSGENIC GOATS EXPRESSING HUMAN GRANULOCYTE–MACROPHAGE COLONY-STIMULATING FACTOR IN THE MAMMARY GLAND 

H. S. Park, S. Y. Jung, S. H. Park, J. K. Park, J. S. Lee, T. S. Kim, S. P. Hong, K. M. Choi, J. G. Seol and K. W. Park

pp. 154-155

 


75 INHIBITION OF NK CELL CYTOTOXICITY IN CLONED MINI-PIGS EXPRESSING HUMAN LEUKOCYTE ANTIGEN-G1 (HLA-G1) GENE  

K. W. Park, G. S. Han, K. M. Choi, S. P. Hong, J. Y. Yoo, E. J. Kim, S. H. Kim, T. S. Kim, S. Y. Park, K. S. Jun, K. N. Heo, D. I. Jin, C. S. Park and J. G. Seol

pp. 155-155

 


76 PREOVULATORY EMBRYO TRANSFER INCREASES SUCCESS OF PORCINE SOMATIC CLONING 

B. Petersen, A. Lucas-Hahn, E. Lemme, N. Hornen, P. Hassel and H. Niemann

pp. 155-156

 


77 LOSS OF SAND CAT CLONED EMBRYOS DURING EARLY PREGNANCY MAY BE ASSOCIATED WITH ABERRANT Oct-4 EXPRESSION 

C. E. Pope, M. C. Gómez, M. Truhe, M. López, C. Dumas, A. Cole, L. Lyons and B. L. Dresser

pp. 156-156

 


78 BLIND ENUCLEATION OF OOCYTES IS HIGHLY EFFICIENT IN ZONA-FREE BOVINE CLONING  

M. I. Prokofiev, O. I. Stepanov, A. V. Komissarow, T. A. Antipova, M. V. Pinyugina and G. P. Malenko

pp. 156-157

 


79 EFFECTS OF EPIDERMAL GROWTH FACTOR, β-MERCAPTOETHANOL, GLUCOSE, OXYGEN CONCENTRATION, AND FIBROBLAST SUBCULTURE ON THE DEVELOPMENT OF PORCINE NT EMBRYOS  

H. B. Seok, J. H. Quan and S. K. Kim

pp. 157-157

 


80 EFFECT OF MEDIA, SYNCHRONIZATION OF FIBROBLAST CELLS, CULTURE TIME, OXYGEN CONCENTRATION, AND ACTIVATION ON NUCLEAR-TRANSFERRED PORCINE OOCYTES  

J. H. Quan, H. B. Seok and S. K. Kim

pp. 157-158

 


81 MOUSE CLONING BY USING A LASER-ASSISTED ZONA OPENING AND ELECTRO-FUSION TECHNIQUE  

R. Rungsiwiwut, A. Thongphakdee, P. Numchaisrika, P. Virutamasen and M. Techakumphu

pp. 158-158

 


82 EFFECT OF DONOR CELL TRANSFECTION EVENTS ON EMBRYO AND FETAL SURVIVAL IN CLONING 

D. F. Salamone, C. B. Santos, J. L. Barañao, L. Bussmann, J. Artuso, C. J. Munar, G. Berra, M. Criscuolo and C. Melo

pp. 158-159

 


83 IN VITRO DEVELOPMENTAL ABILITIES OF PORCINE CLONED EMBRYOS RECONSTITUTED WITH CELL NUCLEI OF GENETICALLY TRANSFORMED FETAL FIBROBLASTS CYTOMETRICALLY DIAGNOSED ON CELL CYCLE AND APOPTOSIS  

M. Samiec, M. Skrzyszowska, M. Bochenek, D. Lipinski and R. Slomski

pp. 159-159

 


84 GENE EXPRESSION DURING BLASTOCYST-TO-ELONGATED STAGE IN BOVINE EMBRYOS DERIVED FROM SOMATIC CELL NUCLEAR TRANSFER  

K. Sawai, S. Moriyasu, H. Hirayama, A. Minamihashi and S. Onoe

pp. 159-160

 


85 PREGNANCIES AND VIABILITY OF THE OFFSPRING AFTER TRANSFER OF HANDMADE CLONED PORCINE EMBRYOS  

M. Schmidt, I. B. Bøgh, Y. Du, Y. Zhang, J. Li, S. Purup and G. Vajta

pp. 160-160

 


86 CREATION OF PORCINE TRANSGENIC NUCLEAR-TRANSFERRED EMBRYOS RECONSTRUCTED WITH EGFP-EXPRESSING ADULT DERMAL FIBROBLAST CELLS ANALYZED ON APOPTOSIS 

M. Skrzyszowska, M. Samiec, Z. Smorag, D. Lipinski and R. Slomski

pp. 160-161

 


87 EVALUATION OF MITOCHONDRIAL FUNCTION IN SINGLE CLONED MINIATURE PIG EMBRYOS BY MEASURING OXYGEN CONSUMPTION  

S. Sugimura, M. Yokoo, K.-I. Yamanaka, T. Wakai, H. Abe, T. Shoji, H. Sasada, J. Kobayashi, H. Abe and E. Sato

pp. 161-161

 


88 CELL CYCLE SYNCHRONIZATION OF FIBROBLASTS DERIVED FROM TRANSGENIC CLONED CATTLE EAR SKIN: EFFECTS OF SERUM STARVATION, ROSCOVITINE, AND CONTACT INHIBITION  

X.-Z. Sun, S.-H. Wang, Y.-H. Zhang, Y.-P. Dai and N. Li

pp. 161-162

 


89 ZYGOTICALLY ACTIVATED GENES ARE SUPPRESSED IN MOUSE NUCLEAR-TRANSFERRED EMBRYOS 

T. Suzuki, N. Minami and H. Imai

pp. 162-162

 


90 GERM-LINE TRANSMISSION OF DONOR MITOCHONDRIAL DNA IN NUCLEAR TRANSFER-DERIVED COWS  

K. Takeda, K. Kaneyama, M. Tasai, S. Akagi, M. Yonai, N. Miyashita, A. Onishi, T. Tagami, K. Nirasawa and H. Hanada

pp. 162-162

 


91 PRODUCTION OF A CLONED BOER GOAT (CAPRA HIRCUS) BY SOMATIC CELL NUCLEAR TRANSFER  

Y. Tao, W. Han, M. Zhang, J. Ding and X. Zhang

pp. 163-163

 


92 INTER-GENERIC MARBLED CAT AND FLAT-HEADED CAT CLONED BLASTOCYSTS GENERATED FROM DOMESTIC CAT AND RABBIT OOCYTES  

A. Thongphakdee, P. Numchaisrika, P. Ratanakorn, S. Kamolnorranath, K. Chatdarong and M. Techakumphu

pp. 163-163

 


93 EFFECT OF SEASON ON QUALITY OF OOCYTES, RESULTS OF IN VITRO MATURATION, AND SOMATIC CELL NUCLEAR TRANSFER IN SWAMP BUFFALO  

N. T. Uoc, F. de Rennis, N. H. Duc, L. C. Bui, N. V. Hanh, N. V. Linh, N. T. Thanh, Q. X. Huu, D. D. Long and B. X. Nguyen

pp. 163-164

 


94 PRODUCTION OF AN α1,3-GALACTOSYLTRANSFERASE GENE KNOCKOUT CALF USING A SINGLE TARGETING VECTOR AND REJUVENATED CELL LINES 

M. Urakawa, Y. Sendai, T. Sawada, Y. Shinkai, A. Ideta, K. Kubota, H. Hoshi and Y. Aoyagi

pp. 164-164

 


95 OBSERVATION OF CHROMOSOME DECONDENSATION, HISTONE H3 MODIFICATION, AND HP1 PROTEIN IN MOUSE CLONED EMBRYOS FOLLOWING INHIBITION OF HISTONE DEACETYLATIONS AND CYCLIN-DEPENDENT KINASE  

N. Van Thuan, B. Hong-Thuy, S. Wakayama, S. Kishigami, H. Ohta, T. Hikichi, E. Mizutani and T. Wakayama

pp. 164-165

 


96 TRICHOSTATIN A IMPROVED THE QUALITY OF RABBIT NUCLEAR TRANSFER EMBRYOS 

J. Xu, L.-Y. Sung, J. Zhang, X. Tian, Y. E. Chen, X. Yang and F. Du

pp. 165-165

 


97 CLONED CALF PRODUCTION BY AGGREGATION OF SOMATIC CELL NUCLEAR-TRANSFERRED EMBRYOS 

D. Yamaguchi, W. Suzuki, N. Adachi, S. Akagi, S. Watanabe and S. Takahashi

pp. 166-166

 


98 BLASTOCYST DEVELOPMENT RATE OF CLONED CAT EMBRYOS USING SERIAL CLONING  

X. J. Yin, H. S. Lee, E. G. Choi, X. F. Yu, B. H. Choi, M. S. Piao, D. H. Oh, S. S. Lee and I. K. Kong

pp. 166-166

 


99 AMOUNT OF TELOMERIC DNA IN GROWING CLONED CATTLE, THEIR PROGENY, AND THEIR ORGANS  

B. C. Yang, G. S. Im, Y. H. Kim, D. H. Kim, S. H. Bae, Y. G. Ko, H. H. Seong, S. H. Shon and B. S. Yang

pp. 167-167

 


100 EFFECT OF DIFFERENT POST-ACTIVATION TREATMENTS ON THE DEVELOPMENT OF SOMATIC CELL NUCLEAR TRANSFER PIG EMBRYOS  

H. Y. Yong, K. Song and E. Lee

pp. 167-167

 


101 DEVELOPMENTAL ARREST AND CYTOSKELETAL ANOMALIES OF RAT EMBRYOS RECONSTRUCTED BY SOMATIC CELL NUCLEAR TRANSFER 

J. G. Yoo, S.-P. Demers, L. Lian and L. C. Smith

pp. 168-168

 


102 RABBIT CLONING: HISTONE ACETYLATION STATUS OF DONOR CELLS AND CLONED EMBRYOS  

V. Zakhartchenko, F. Yang, R. Hao and E. Wolf

pp. 168-168

 


103 BIRTH OF CLONED PIGLETS DERIVED FROM AN OPTIMIZED IN VITRO BLASTOCYST PRODUCTION SYSTEM BY TRICHOSTATIN A TREATMENT  

Y.-H. Zhang, Y.-T. Du, K. Zhang, J. Li, P. M. Kragh, K. Villemoes, A. M. Pederson, M. H. Schmidt, I. B. Bøgh, S. Purup, L. Bolund, A. L. Jørgensen, H.-M. Yang, N. Li and G. Vajta

pp. 168-168

 


104 DEVELOPMENTAL POTENTIAL OF CLONED EMBRYOS FROM ADULT AND FETAL OVINE SOMATIC CELLS 

H. M. Zhou and Y. Chen

pp. 169-169

 


105 ALTERING SEMEN EXTENDER AND GLYCEROL CONCENTRATION TO OPTIMIZE RESULTS AFTER CRYOPRESERVATION OF EQUINE SPERMATOZOA  

M. Aceves, J. Scherzer, L. Ray, G. Heusner and R. A. Fayrer-Hosken

pp. 170-170

 


106 USING A BUOYANT DENSITY GRADIENT AND NILE RED STAINING TO EVALUATE THE LIPID CONTENT OF BOS TAURUS AND BOS INDICUS OOCYTES 

C. B. Ballard, C. R. Looney, B. R. Lindsey, J. H. Pryor, J. W. Lynn, K. R. Bondioli and R. A. Godke

pp. 170-171

 


107 DEVELOPMENT OF VITRIFIED BOVINE SECONDARY AND PRIMORDIAL FOLLICLES IN SCID MICE  

R. M. Bao, E. Yamasaka, M. Moniruzzaman, A. Hamawaki, M. Yoshikawa and T. Miyano

pp. 171-171

 


108 EFFECT OF CAFFEINE TREATMENT ON MPF AND MAPK ACTIVITY AND PARTHENOGENETIC ACTIVATION OF VITRIFIED MII OVINE OOCYTES  

L. Bogliolo, F. Ariu, S. Succu, S. Fois, G. Leoni and S. Ledda

pp. 171-172

 


109 EFFECTS OF VITRIFICATION PROCEDURES ON SUBSEQUENT DEVELOPMENT AND ULTRASTRUCTURE OF IN VITRO-MATURED SWAMP BUFFALO (BUBALUS BUBALIS) OOCYTES  

D. Boonkusol, T. Faisaikarm, A. Dinnyes and Y. Kitiyanant

pp. 172-172

 


110 COMPARISON OF ANDROMED®, BIOXCELL®, AND BOTU-BOV® EXTENDERS FOR CRYOPRESERVATION OF BULL SEXED SEMEN 

M. Q. Braga, R. V. R. Franco, L. F. Rodrigues, G. Galeli, K. M. Oliveira, F. A. C. Reis, M. F. A. Nishikawa and E. P. Moura

pp. 172-172

 


111 SLOW FREEZING AND VITRIFICATION OF IN VITRO-MATURED DOMESTIC CAT OOCYTES 

J. Braun, C. Otzdorff, T. Tsujioka and S. Hochi

pp. 173-173

 


112 VIABILITY AND APOPTOSIS OF BOVINE FIBROBLASTS FROZEN IN A CONVENTIONAL (–20°C) OR LOW-TEMPERATURE (–80°C) FREEZER 

L. Chacón, M. C. Gómez, J. Jenkins, S. P. Leibo, G. Wirtu, B. L. Dresser and C. E. Pope

pp. 173-174

 


113 RETINOIC ACID DOES NOT PROTECT AGAINST THE SELECTIVE DAMAGE TO THE BOVINE INNER CELL MASS INFLICTED BY VITRIFICATION  

C. Díez, A. Rodríguez, C. De Frutos, J. N. Caamaño, N. Facal and E. Gómez

pp. 174-174

 


114 CRYOTOP VITRIFICATION FOR IN VITRO-PRODUCED BUFFALO (BUBALUS BUBALIS) EMBRYOS  

A. De Rosa, L. Attanasio, L. Boccia, G. Pellerano, G. Campanile and B. Gasparrini

pp. 174-175

 


115 RELATIONSHIP BETWEEN IN VIVO FERTILITY OF MANCHEGA EWES LAPAROSCOPICALLY INSEMINATED WITH FROZEN–THAWED RAM SEMEN AND HETEROLOGOUS CALF IN VITRO FERTILIZATION 

O. Garcia-Alvarez, A. Maroto-Morales, M. D. Perez-Guzman and A. J. Soler

pp. 175-175

 


116 EFFECT OF INCUBATION OF BOVINE EPIDIDYMAL SPERMATOZOA IN SEMINAL PLASMA PRIOR TO CRYOPRESERVATION 

C. A. Guerrero, J. A. Jenkins, J. W. Lynn, K. R. Bondioli and R. A. Godke

pp. 175-176

 


117 EFFECT OF STEPWISE DILUTION ON THE VIABILITY OF FROZEN–THAWED BOVINE OOCYTES MATURED IN VITRO  

A. Hamawaki, S. Hamano, M. Yoshikawa and K. Matsukawa

pp. 176-176

 


118 CRYOPRESERVATION OF CONVENTIONAL AND SEX-SORTED BULL SPERM USING A DIRECTIONAL FREEZING METHOD  

H. Hayakawa, T. Yamazaki, M. Oshi, M. Hoshino, O. Dochi and H. Koyama

pp. 176-177

 


119 OXIDATIVE STRESS DURING THE CRYOPRESERVATION OF BOAR SPERMATOZOA  

M. Hernandez, J. M. Vazquez, E. A. Martinez and J. Roca

pp. 177-177

 


120 SUCCESSFUL PRODUCTION OF PIGLETS DERIVED FROM VITRIFIED MORULAE AND EARLY BLASTOCYSTS WITHOUT DIRECT EXPOSURE TO LIQUID NITROGEN  

Y. Hirayama, M. Ohkubo, K. Misumi and K. Imai

pp. 177-178

 


121 CRYOPRESERVATION OF BOVINE BIOPSIED EMBRYOS UNDER A MAGNETIC FIELD  

A. Ideta, K. Hayama, M. Urakawa, N. Ohwada and Y. Aoyagi

pp. 178-178

 


122 INTRACELLULAR ICE FORMATION IN VITRIFIED MOUSE MORULAE DURING WARMING 

B. Jin, K. Kusanagi, M. Ueda, K. Edashige and M. Kasai

pp. 178-179

 


123 CRYOPROTECTANT EXPOSURE AFFECTS THE PROTEOME OF THE MOUSE METAPHASE II OOCYTE  

M. Larman, M. Katz-Jaffe, C. Sheehan, W. Schoolcraft and D. Gardner

pp. 179-179

 


124 TAXOL™ COULD PROMOTE EMBRYO DEVELOPMENT OF BOVINE OOCYTES VITRIFIED BY OPS 

R. Morató, D. Izquierdo, M. J. Palomo, B. Anguita, A. R. Jiménez-Macedo, J. L. Albarracín, M. T. Paramio and T. Mogas

pp. 179-180

 


125 IN VITRO-PRODUCED BOVINE EMBRYOS VITRIFIED IN GLASS CAPILLARIES USING TCM-199 OR DULBECCO'S PHOSPHATE-BUFFERED SALINE AS VITRIFICATION-WARMING MEDIA  

N. Mucci, G. G. Kaiser, G. Rios, R. H. Alberio, L. B. Ferré and J. Aller

pp. 180-180

 


126 CRYOPRESERVATION OF PORCINE EMBRYOS DERIVED FROM IVM OOCYTES 

N. Nakayama, K. Hiruma, M. Kurome, R. Tomii, S. Ueno, H. Saito, H. Matsunari and H. Nagashima

pp. 180-181

 


127 IN VITRO DEVELOPMENT OF IVF CRYOPRESERVED BOVINE EMBRYOS SUPPORTED BY TCM-199 OR SOFaa CULTURE MEDIUM  

A. C. Nicacio, W. B. Feitosa, M. Rovegno, R. Simões, J. S. de A. Gonçalves, M. E. O. D'Á. Assumpção and J. A. Visintin

pp. 181-181

 


128 IMPROVED POST-THAW MOTILITY, VIABILITY, AND FERTILITY ARE ACHIEVED BY HYDROSTATIC PRESSURE-TREATED BULL SEMEN 

C. Pribenszky, M. Molnár, A. Horváth, G. Kútvölgyi, A. Harnos, O. Szenci, J. Dengg and J. Lederer

pp. 181-182

 


129 IMPROVEMENT OF BOAR SPERM CRYOSURVIVAL BY THE OPTIMIZATION OF CRYOPRESERVATION CONDITIONS 

J. Roca, M. Hernandez, T. Cremades, J. M. Vazquez and E. A. Martinez

pp. 182-182

 


130 EFFECTIVENESS OF BUTYLATED HYDROXYTOLUENE AS EGG YOLK SUBSTITUTE FOR CRYOPRESERVATION OF BOAR SPERMATOZOA 

L. Rodriguez-Vilar, M. Hernandez, C. Lopez-Sanchez, J. M. Vazquez, E. A. Martinez and J. Roca

pp. 182-183

 


131 BOVINE OOCYTE VITRIFICATION IS IMPROVED BY INCREASED PYRUVATE CONCENTRATION AND THE INCLUSION OF HYALURONAN IN THE VITRIFICATION MEDIUM  

C. B. Sheehan, M. G. Larman and D. K. Gardner

pp. 183-183

 


132 SUCCESSFUL EMBRYO TRANSFER OF IN VIVO-PRODUCED RED DEER (CERVUS ELAPHUS) EMBRYOS AFTER CRYOPRESERVATION BY SLOW FREEZING OR VITRIFICATION 

J. P. Soler, G. G. Kaiser, N. Mucci, L. B. Ferre and R. H. Alberio

pp. 183-184

 


133 VITRIFICATION CAUSES DAMAGE OF THE ANTIOXIDANT DEFENSE SYSTEM IN IVM PORCINE OOCYTES  

T. Somfai, M. Ozawa, J. Noguchi, H. Kaneko, K. Ohnuma, N. W. K. Karja, M. Farhudin, N. Maedomari, A. Dinnyes, T. Nagai and K. Kikuchi

pp. 184-184

 


134 CONCEPTION RATES OF IN VITRO-PRODUCED BOVINE EMBRYOS CRYOPRESERVED IN 6% GLYCEROL AND TRANSFERRED BY THE DIRECT METHOD  

N. Takada, S. Hayasaka and K. Chiba

pp. 184-185

 


135 SHORT-TERM CRYOPRESERVATION OF VITRIFIED MOUSE MORULAE AT –79°C 

Y. Takagi, M. Shimizu, M. Morimura, S. Yokomizo, K. Hara and M. Sakamoto

pp. 185-185

 


136 CLOSED SYSTEM OF GEL-LOADING-TIP VITRIFICATION FOR IN VITRO-PRODUCED BOVINE BLASTOCYSTS BEFORE AND AFTER BIOPSY 

K. Tominaga, F. Iwaki, E. Yamaguchi, Y.-M. Dou, I. Kijihana and S. Hochi

pp. 185-186

 


137 EFFECT OF POST-IVF DEVELOPMENTAL KINETICS ON SURVIVAL AND QUALITY OF VITRIFIED–WARMED DOMESTIC CAT BLASTOCYSTS 

T. Tsujioka, C. Otzdorff, J. Braun and S. Hochi

pp. 186-186

 


138 EFFECT OF SEMINAL PLASMA ON CHILLING AND FREEZING OF CANINE SPERMATOZOA  

I. Yu, Y. J. Kim, I. S. Kim, S. P. Leibo and N. Songsasen

pp. 187-187

 


139 OXIDATIVE STRESS INDUCED CHANGES OF GLUTATHIONE CONCENTRATION IN IN VITRO-PRODUCED BOVINE BLASTOCYSTS  

M. Aksoy, C. Wrenzycki, D. Herrmann and H. Niemann

pp. 187-187

 


140 LOCALIZATION OF PRIMORDIAL GERM CELLS IN DAY 21 BOVINE EMBRYOS 

N. I. Alexopoulos, N. T. D'Cruz and P. Maddox-Hyttel

pp. 188-188

 


141 INDICATIONS FOR DNA DOUBLE-STRAND BREAK REPAIR IN EARLY BOVINE EMBRYOS 

A. Brero, D. Koehler, T. Cremer, E. Wolf and V. Zakhartchenko

pp. 188-188

 


142 RELATIONSHIP BETWEEN APOPTOSIS IN BOAR SEMEN AND DNA FRAGMENTATION IN PORCINE EMBRYOS 

M. Bryla, M. Trzcinska and Z. Smorag

pp. 188-189

 


143 DEVELOPMENTAL EFFICIENCY IMPROVEMENTS OF NUCLEAR TRANSFER EMBRYOS BY REPROGRAMMING SOMATIC CELLS USING TESTIS-DERIVED CELL EXTRACTS  

H.-Y. Choi, C. Won, B.-W. Kim, Y.-J. Kang, G.-H. Kang, Y. Oh, E.-R. Lee, S. Roh and S.-G. Cho

pp. 189-189

 


144 Oct-4 EXPRESSION PATTERN IN THE EQUINE EMBRYO  

Y. H. Choi, H. D. Harding, A. D. Obermiller and K. Hinrichs

pp. 189-190

 


145 THE METHYLATION PATTERNS OF POTENTIAL DIFFERENTIALLY METHYLATED REGIONS IN BOVINE Xist, Impact, NDN, AND H19 GENES 

N. T. D'Cruz, K. J. Wilson and M. K. Holland

pp. 190-190

 


146 LECTIN DOLICHOS BIFLOURUS AGGLUTININ (DBA) IS A SPECIFIC MARKER for PRIMITIVE GERM CELLS IN NEONATAL PORCINE TESTIS 

S. Goel, M. Sugimoto, S. Kume, M. Yamada, N. Minami and H. Imai

pp. 190-190

 


147 GENE KNOCKDOWN OF MATERNAL- AND EMBRYONIC-EXPRESSED GREEN FLUORESCENT PROTEIN IN MURINE EMBRYOS BY THE INJECTION OF A SHORT INTERFERING RNA (siRNA) 

K. Iqbal, W. A. Kues, J. W. Carnwath and H. Niemann

pp. 191-191

 


148 EFFECT OF FOLLISTATIN TREATMENT POST-FERTILIZATION ON TIME TO FIRST CLEAVAGE, DEVELOPMENT TO THE BLASTOCYST STAGE, AND CELL ALLOCATION OF IN VITRO-PRODUCED BOVINE EMBRYOS  

K. B. Lee, A. Bettegowda, J. J. Ireland and G. W. Smith

pp. 191-192

 


149 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED PROTEINS IN THE UMBILICAL CORDS OF TERM AND PRE-TERM (35–36 WEEKS) INFANTS USING PROTEOMICS 

S. Y. Lee, S. K. Cho, D. N. Kwon, J. Y. Park and J. H. Kim

pp. 192-192

 


150 THE DISTRIBUTION PATTERN OF PRIMORDIAL GERM CELLS IN EARLY CHICK EMBRYOS  

Y. Nakamura, Y. Yamamoto, F. Usui, T. Ono, K. Takeda, K. Nirasawa, H. Kagami and T. Tagami

pp. 192-193

 


151 LIPID COMPOSITION OF FAT DROPLETS OF IN VIVO- AND IN VITRO PRODUCED PORCINE EMBRYOS 

M. Romek, B. Gajda, E. Krzysztofowicz and Z. Smorag

pp. 193-193

 


152 DIELECTROPHORETIC BEHAVIOR OF BOVINE OOCYTES AND ZYGOTES IN RELATION TO DEVELOPMENT AND TRANSCRIPTIONAL ABUNDANCE  

D. Salilew-Wondim, F. Rings, M. Hoelker, D. Jennen, E. Tholen, M. A. Sirard, K. Schellander and D. Tesfaye

pp. 193-194

 


153 IMMUNOCYTOCHEMICAL STUDIES OF OCT-4, SOX-2, AND β-TUBULIN III EXPRESSION IN EARLY PORCINE EMBRYOS  

K. Schauser, T. S. Sundkvist, M. Vejlsted and P. Maddox-Hyttel

pp. 194-194

 


154 UNDERNUTRITION DURING THE PERICONCEPTIONAL PERIOD IN EWES AFFECTS THE BLOOD PRESSURE AND WEIGHT OF THEIR NEONATAL LAMBS  

N. A. Smith, P. Lonergan, P. Duffy, T. F. Crosby, P. Quinn, F. McAuliffe and A. C. O. Evans

pp. 194-195

 


155 LAPAROSCOPIC EVALUATION OF OVARIAN REACTION TO HORMONE STIMULATION IN CYNOMOLGUS MONKEYS (MACACA FASCICULARIS)  

H. Tsuchiya, C. Iwatani, J. Yamasaki, J. N. Okahara, N. Okahara and R. Torii

pp. 195-195

 


156 DIFFERENTIAL REORGANIZATION OF HISTONE ACETYLATION THROUGH ATP-DEPENDENT REMODELING FACTOR DURING PRONUCLEAR FORMATION IN PORCINE ZYGOTIC CHROMATINS 

G. Wee, D.-B. Koo, J.-S. Kim, B.-S. Song, J.-S. Park, X.-L. Jin, Y.-Y. Lee, Y.-M. Han and K.-K. Lee

pp. 195-195

 


157 DYNAMIC CHANGES IN LOCALIZATION OF HP1α DURING BOVINE EMBRYOGENESIS 

K. J. Wilson, S. Prashadkumar, M. A. Cooney, A. J. French, D. A. Jans, P. J. Verma, M. K. Holland and N. T. D'Cruz

pp. 196-196

 


158 LEADING BLASTOMERE OF A 2-CELL-STAGE PORCINE PARTHENOGENETIC EMBRYO CONTRIBUTES TO TROPHECTODERM FIRST 

C. Won, S. K. Park, Y.-J. Choi, H. Kang and S. Roh

pp. 196-196

 


159 LOCALIZATION OF CLAUDIN FAMILY PROTEINS IN PIG EMBRYOS DURING PRE-IMPLANTATION DEVELOPMENT 

S. Xu, J. Lee, H. Harayama and M. Miyake

pp. 197-197

 


160 DETERMINATION OF THE INCIDENCE OF EMBRYONIC MORTALITY IN SYNCHRONIZED EWES DURING THE BREEDING SEASON  

H. Erdem, M. K. Saribay and T. Tekeli

pp. 197-197

 


161 EFFECT OF MELOXICAM TREATMENT ON PREGNANCY RATES IN HOLSTEIN HEIFERS  

A. Guzeloglu and H. Erdem

pp. 197-198

 


162 ESTRUS SYNCHRONIZATION, ARTIFICIAL INSEMINATION, AND PREGNANCY DIAGNOSIS IN WATER BUFFALOES (BUBALUS BUBALIS) 

N. V. Hanh, Q. X. Huu, N. T. Uoc, B. X. Nguyen, J. Sulon, N. M. Sousa and J. F. Beckers

pp. 198-198

 


163 FUNCTIONAL COMPARISON OF BOVINE TROPHOBLASTIC VESICLES DERIVED FROM FRESHLY COLLECTED CONCEPTUSES AND SERIALLY PASSAGED TROPHOBLAST CELLS  

Y. Hashiyada, H. Takahashi, M. Asada, N. Sakuta, S. Furuyashiki, S. Yamaya, T. Oike, K. Konishi and M. Takahashi

pp. 198-199

 


164 POTENTIAL REGULATION OF PERI-IMPLANTATION OVINE CONCEPTUS GROWTH AND DIFFERENTIATION BY THE WNT SIGNALING SYSTEM 

K. Hayashi, R. C. Burghardt, F. W. Bazer and T. E. Spencer

pp. 199-199

 


165 EXPRESSION OF CALCIUM TRANSPORTER 1 GENE IN THE UTERUS OF RATS DURING PREGNANCY 

B.-M. Lee, G.-S. Lee and E.-B. Jeung

pp. 199-200

 


166 DIFFERENTIAL EXPRESSION OF AMPHIREGULINE, EPIDERMAL GROWTH FACTOR, AND CALBINDIN-D9k IN THE UTERUS OF RODENTS AND PIGS DURING IMPLANTATION 

G.-S. Lee, M.-H. Kim, S.-H. Hyun and E.-B. Jeung

pp. 200-200

 


167 EXPRESSION OF INTERFERON τ IN BOVINE EMBRYOS DERIVED FROM PARTHENOGENETIC ACTIVATION  

A. Minamihashi, S. Moriyasu, H. Takahashi, H. Hirayama, M. Geshi, S. Onoe and K. Sawai

pp. 200-200

 


168 ESTABLISHMENT OF TROPHOBLAST STEM CELLS FROM MOUSE ANDROGENETIC AND PARTHENOGENETIC EMBRYOS 

H. Ogawa, N. Shindo, S. Tanaka and T. Kono

pp. 201-201

 


169 PREDICTION OF THE OPTIMAL DAY OF MATING IN THE BITCH USING A BOVINE PLASMA PROGESTERONE ELISA KIT  

M. Seki, Y. Yamazaki, N. Watanabe, K. Ishii, Y. Kinoshita, T. Aihara, S. Takeiri and T. Otoi

pp. 201-201

 


170 EXPRESSION PROFILE AND PROTEIN SECRETION OF BOVINE INTERFERON τ ON DAYS 12 TO 28 IN EARLY PREGNANCY 

H. Takahashi, K. Sawai, A. Minamihashi, S. Kageyama, S. Moriyasu, H. Hirayama, S. Onoe and M. Geshi

pp. 201-202

 


171 CELLULAR EXPRESSION, LOCALIZATION, AND SECRETION OF OVINE Mx2 (oMx2) IN OVINE GLANDULAR EPITHELIAL CELLS (oGE)  

K. Toyokawa, A. M. Assiri and T. L. Ott

pp. 202-202

 


172 DOES ESTRADIOL-17β CAUSE CYCLE-DEPENDENT MODULATIONS OF UTERINE MILK PROTEIN IN BOVINE ENDOMETRIUM?  

S. E. Ulbrich, K. Gross, S. Schmidt, H. Blum, R. Rottmayer, S. Hiendleder, T. Fröhlich, G. J. Arnold, E. Wolf, H. H. D. Meyer and S. Bauersachs

pp. 202-203

 


173 HINDERING OF CLEAVAGE TIMING IN BOVINE PARTHENOTES DURING THE HOT SEASON  

A. Aroyo, S. Yavin, Z. Roth and A. Arav

pp. 203-203

 


174 THE EFFECT OF DIFFERENT OVARY TRANSPORT TEMPERATURES ON IN VITRO DEVELOPMENT AND POST-THAW SURVIVAL OF BOVINE EMBRYOS  

S. R. Cho, S. H. Choi, H. J. Kim, C. Y. Choe, H. J. Jin and D. S. Son

pp. 203-204

 


175 DETECTION OF LIPID GRANULES ON IVF BLASTOCYSTS MATURED IN APO-TRANSFERRIN IN KOREAN NATIVE COWS (HANWOO)  

S. H. Choi, S. R. Cho, M. H. Han, H. J. Kim, C. Y. Choe, D. S. Son, J. S. Woo, B. D. Sang, Y. G. Chung and H. Hoshi

pp. 204-204

 


176 EFFECT OF LEUKEMIA INHIBITORY FACTOR FROM HUMAN AND MOUSE ORIGIN ON THE DEVELOPMENT OF BOVINE EMBRYOS PRODUCED IN VITRO  

C. De Frutos, A. Rodríguez, C. Díez, J. N. Caamaño, N. Facal, P. Duque, C. García-Ochoa and E. Gómez

pp. 204-205

 


177 ISOLATION AND CULTURE OF MOUSE ENDOMETRIAL CELLS IN A THREE-DIMENSIONAL CULTURE SYSTEM 

T.-Y. Fu, P.-C. Tang and J.-C. Ju

pp. 205-205

 


178 IN VITRO CULTURE OF BOVINE EMBRYOS WITH NEUROTROPHINS  

E. Gómez, A. Rodríguez, C. De Frutos, J. N. Caamaño, N. Facal and C. Díez

pp. 205-206

 


179 EMBRYO DEVELOPMENT AND EXPERIMENT OUTCOME CAN BE COMPROMISED WITHOUT APPROPRIATE QUALITY CONTROL 

D. K. Gardner, D. W. Linck and C. B. Sheehan

pp. 206-206

 


180 DEVELOPMENTAL CAPACITY OF SINGLE CULTIVATED EMBRYOS IN SMALL AND BIG DROPLETS 

I. G. F. Goovaerts, J. B. P. De Clercq, M. Nichi and P. E. J. Bols

pp. 206-207

 


181 DIFFERENCES EXIST IN ZONA PELLUCIDA HARDNESS BETWEEN IN VIVO- AND IN VITRO-GENERATED BOVINE EMBRYOS  

F. Itoi, T. Himaki, C. Kubota, J. Hirose, M. Miyamura, S. Hamano, Y. Murayama, S. Omata and M. Yoshida

pp. 207-207

 


182 INTERACTION OF MICROTUBULES AND MICROFILAMENTS WITH DISTRIBUTION OF MITOCHONDRIA IN TWO-CELL HAMSTER EMBRYOS  

K. Kabashima, M. Matsuzaki and H. Suzuki

pp. 207-208

 


183 ADDITION OF DIPHENYLENEIODONIUM OR DEHYDROEPIANDROSTERONE TO CULTURE MEDIA INHIBITS REACTIVE OXYGEN SPECIES PRODUCTION DURING THE EARLY CLEAVAGE STAGES OF IN VITRO-PRODUCED PORCINE EMBRYOS  

N. W. K. Karja, K. Kikuchi, M. Ozawa, M. Fahrudin, T. Somfai, M. Nakai, K. Ohnuma, J. Noguchi, H. Kaneko and T. Nagai

pp. 208-208

 


184 EFFECT OF CONCENTRATION AND EXPOSURE DURATION OF FETAL BOVINE SERUM ON PARTHENOGENETIC DEVELOPMENT OF PORCINE FOLLICULAR OOCYTES 

H. J. Kim, S. R. Cho, C. Y. Choe, S. H. Choi, D. S. Son, S. J. Kim, Y. G. Kim, M. H. Han, I. S. Ryu, I. C. Kim, I. H. Kim and K. S. Im

pp. 208-209

 


185 PGI2 ANALOG INDUCES HIGH-QUALITY PORCINE BLASTOCYSTS IN VITRO  

J.-S. Kim, G. Wee, B.-S. Song, J.-S. Park, X.-L. Jin, Y.-Y. Lee, Y.-M. Han, K.-K. Lee and D.-B. Koo

pp. 209-209

 


186 THE EFFECT OF MATRIGEL ON THE DEVELOPMENT OF IN VITRO-FERTILIZED PORCINE EMBRYOS  

S.-W. Kim, M.-J. Lee, B.-C. Yang, G.-S. Im, H.-H. Seong, B.-S. Yang, H.-T. Cheong and D.-H. Kim

pp. 209-210

 


187 INCREASED NUMBER OF EXPANDED BOVINE BLASTOCYSTS IN SOF AND CR1 RELATIVE TO KSOM  

D. M. Kohl, R. L. Monson, L. E. Enwall and J. J. Rutledge

pp. 210-210

 


188 THE IMPACT OF OIL SOURCE AND TYPE OF GLUTAMINE ON MEDIA EQUILIBRATION TIMES AND EMBRYO DEVELOPMENT  

D. W. Linck and D. K. Gardner

pp. 210-211

 


189 EFFECT OF PROGESTERONE AND EPIDERMAL GROWTH FACTOR ON IN VITRO-PRODUCED EIGHT-CELL BOVINE EMBRYOS IN A SERUM-FREE CULTURE MEDIUM 

B. Merlo, E. Iacono and G. Mari

pp. 211-211

 


190 INFLUENCE OF IN VITRO CULTURE OF BOVINE EMBRYOS ON THE STRUCTURE OF THE ZONA PELLUCIDA  

E. Mertens, U. Besenfelder, M. Gilles, M. Hölker, F. Rings, V. Havlicek, K. Schellander and A. Herrler

pp. 211-212

 


191 ALLEVIATION OF THE TWO-CELL BLOCK OF ICR, ddY, AND AKR MOUSE EMBRYOS BY BOVINE SERUM ALBUMIN  

K. Ono, R. Ohishi, H. Imai and M. Yamada

pp. 212-212

 


192 THE EFFECT OF ZWITTERONIC BUFFERS AND PBS ON GENE TRANSCRIPTION OF BOVINE IN VIVO- AND IN VITRO-DERIVED EMBRYOS  

A. T. Palasz, P. Beltrán Breña, S. Pérez-Garnelo, S. Fuentes, J. De la Fuente1 and A. Gutiérrez-Adán

pp. 212-213

 


193 DIFFERENTIAL EFFECTS OF CULTURE ON APOPTOTIC GENE EXPRESSION IN THE PRE-IMPLANTATION CLONED EMBRYOS OF MINIATURE PIG  

M. R. Park, I. S. Hwang, H. J. Moon, J. H. Shim, D. H. Kim, B. C. Yang, H. J. Cheong, Y. G. Ko, B. S. Yang and G. S. Im

pp. 213-213

 


194 ANTIOXIDATIVE EFFECT OF BROWN ALGAE PHLOROTANNINS ON REACTIVE OXYGEN SPECIES GENERATION AND EMBRYO DEVELOPMENT OF PORCINE PARTHENOGENETIC EMBRYOS UNDER OXIDATIVE AND HEAT-STRESSED CONDITIONS  

M. Takahashi, K. Nagayama, M. Sakatani, S. Kobayashi, K. Morishita and M. Asakawa

pp. 213-214

 


195 SODIUM SELENITE INCREASES DEVELOPMENTAL ABILITY AND DECREASES APOPTOSIS IN PORCINE PARTHENOTES 

S. J. Uhm, M. K. Gupta, J. H. Yang and H. T. Lee

pp. 214-214

 


196 A NEW APPROACH TO PREDICT MOUSE EMBRYONIC DEVELOPMENTAL COMPETENCE USING A TIME-LAPSE SYSTEM AND THE 'SHORTEST HALF' analysis procedure 

S. Yavin, A. Aroyo, Z. Roth and A. Arav

pp. 214-215

 


197 EFFECTS OF HYALURONAN ON OXYGEN CONSUMPTION AND ATP CONTENT IN PIG BLASTOCYSTS PRODUCED IN VITRO  

K. Yoshioka, M. Yokoo, T. Ozawa, C. Suzuki, H. Abe and H. Rodríguez-Martínez

pp. 215-215

 


198 BENEFICIAL EFFECT OF GHRELIN ON IN VITRO DEVELOPMENT OF PORCINE IN VITRO-FERTILIZED AND PARTHENOGENETIC EMBRYOS  

K. Zhang, H. X. Wei, S. H. Wang, Y. H. Zhang, Y. Li, Y. P. Dai and N. Li

pp. 215-216

 


199 EVIDENCE FOR A NOVEL PERTURBATION IN CLONED FETUSES: MITOCHONDRIAL DNA DEPLETION  

S. Hiendleder, D. Bebbere, S. E. Ulbrich, V. Zakhartchenko, M. Weppert, H.-D. Reichenbach, H. H. D. Meyer, S. Ledda and E. Wolf

pp. 216-216

 


200 EFFECT OF OOCYTE SIZE AND MICROMANIPULATION TECHNIQUES (ICSI AND EMBRYO BIOPSY) ON GOAT EMBRYO QUALITY 

A. R. Jimenez-Macedo, B. Anguita, D. Izquierdo, R. Romaguera, R. Morato, T. Mogas and M. T. Paramio

pp. 217-217

 


201 FACTORS AFFECTING THE SURVIVAL OF BIOPSIED RHESUS MACAQUE EMBRYOS 

H. M. Kubisch and M. S. Ratterree

pp. 217-217

 


202 INNER CELL MASS EXCHANGE IN SHEEP EMBRYOS 

P. Loi, K. Matsukawa, C. Galli and G. Ptak

pp. 218-218

 


203 PRODUCTION OF ANDROGENETIC SHEEP BLASTOCYSTS 

K. Matsukawa, M. Y. Turco, G. Ptak and P. Loi

pp. 218-219

 


204 RELATIONSHIP BETWEEN RESPIRATORY ACTIVITY AND THE PREGNANCY RATE OF BISECTED BOVINE EMBRYOS IN VIVO 

S. Moriyasu, H. Hirayama, K. Sawai, S. Kageyama, S. Aoyagi, H. Shiku, T. Matsue, H. Abe, M. Kacchi, H. Hoshi and A. Minamihashi

pp. 219-219

 


205 INCREASED PREGNANCY RATES OF POOR QUALITY BOVINE EMBRYOS BY ASSISTED HATCHING  

A. Taniyama, Y. Watanabe, Y. Nisino and T. Inoue

pp. 219-220

 


206 EFFECTS OF A COMBINATION OF A PRID, PGF AND eCG, ON ESTRUS SYNCHRONIZATION AND PREGNANCY RATE FOLLOWING EMBRYO TRANSFER IN HOLSTEIN HEIFERS  

Y. Aoyagi, A. Ideta, M. Matsui, K. Hayama, M. Urakawa, A. Miyamoto and Y. Miyake

pp. 220-220

 


207 INFLUENCE OF THE INTERVAL BETWEEN THAWING TO TRANSFER ON PREGNANCY RATES OF FROZEN–THAWED DIRECT-TRANSFER EMBRYOS  

G. A. Bo, L. C. Peres, D. Pincinato, M. de la Rey and R. Tribulo

pp. 220-221

 


208 EFFECT OF TWO CATTLE EMBRYO CO-CULTURE SYSTEMS ON CALVING RATE 

A. M. Duszewska, J. Wojdan, W. Gawron, E. Wenta-Muchalska, B. Was, A. Wisniewska, A. Chromik and Z. Reklewski

pp. 221-221

 


209 VIABILITY OF PORCINE EMBRYOS VITRIFIED BY THE METAL MESH VITRIFICATION METHOD AFTER SURGICAL OR NONSURGICAL TRANSFER 

Y. Fujino, Y. Nakamura, H. Kobayashi, S. Nakano, C. Suzuki and K. Yoshioka

pp. 221-222

 


210 FACTORS AFFECTING CONCEPTION RATE IN LACTATING HOLSTEIN RECIPIENTS  

D. T. G. Jardina, C. A. Rodrigues and J. L. M. Vasconcelos

pp. 222-222

 


211 A COMPARISON OF PREGNANCY MAINTENANCE AFTER EMBRYO TRANSFER OF DAY 7 OR 13 BOVINE EMBRYOS AND THE RELATIONSHIP BETWEEN INTERFERON-TAU PRODUCTION OF DAY 13 BOVINE EMBRYOS AND PREGNANCY SUCCESS  

K. Kimura, N. Hayashi and H. Iwata

pp. 222-223

 


212 EFFECT OF OVULATION STATUS ON PREGNANCY RATES IN RECIPIENT GILTS TRANSFERRED WITH CLONED EMBRYO TRANSFER  

J. H. Lee, Y. W. Jeung, S. H. Hyun, E. S. Lee and E. B. Jeung

pp. 223-223

 


213 THE EFFECT OF eCG TREATMENT IN AN ESTRUS SYNCHRONIZATION PROTOCOL ON PREGNANCY RATES AFTER EMBRYO TRANSFER IN HOLSTEIN HEIFERS 

T. Okazaki, E. Sasaki, K. Hasegawa, T. Takani and S. Abe

pp. 223-224

 


214 EFFECT OF TIMING OF PGF, eCG, AND ESTRADIOL BENZOATE IN RECIPIENTS SYNCHRONIZED WITH A PROGESTIN-BASED PROTOCOL FOR FIXED-TIME EMBRYO TRANSFER  

L. C. Peres, D. Pincinato, L. Cutaia, R. Tribulo and G. A. Bo

pp. 224-224

 


215 COMPARISON BETWEEN CONVENTIONAL DIRECT TRANSFER FREEZING AND VITRIFICATION FOR THE CRYOPRESERVATION OF IN VIVO EMBRYOS FROM BRAHMAN CATTLE 

J. H. Pryor, C. R. Looney, D. Walker, G. E. Seidel, Jr, J. F. Hasler, D. C. Kraemer and S. Romo

pp. 224-225

 


216 THE RELATIONSHIP BETWEEN OXYGEN CONSUMPTION RATE AND PREGNANCY RATE OF BOVINE EMBRYOS  

N. Sakagami, K. Akiyama and Y. Nakazawa

pp. 225-225

 


217 A NEW APPROACH TO CRYOPRESERVATION OF LARGE EQUINE EMBRYOS BY VITRIFICATION AFTER BLASTOCOEL MICROMANIPULATION 

J. Scherzer, R. A. Fayrer-Hosken, L. Ray and G. Heusner

pp. 225-226

 


218 BREEDING TRIAL OF CONGENITAL HYDROCEPHALUS OF JAPANESE BLACK CATTLE USING EMBRYO TRANSFER  

Y. Shioya, T. Suzuki, Y. Sato, Y. Yoshikawa, Y. Hosokawa, K. Fukunari, Y. Sugimoto, T. Watanabe and N. Takenouchi

pp. 226-226

 


219 COMPARISON OF ESTRUS–OVULATION SYNCHRONIZATION TREATMENTS USING HOLSTEIN HEIFERS AS EMBRYO TRANSFER RECIPIENTS  

H. Ushijima, N. Tatsuno, Y. Hara, T. Mori, H. Murata and M. Ohno

pp. 226-227

 


220 CpG METHYLATION PROFILE OF ADIPOGENIC AND ENDOTHELIAL GENE PROMOTERS IN HUMAN ADIPOSE STEM CELLS SUGGESTS A RESTRICTIVE ENDOTHELIAL DIFFERENTIATION POTENTIAL 

A. C. Boquest, A. Noer, A. L. Sørensen, K. Vekterud and P. Collas

pp. 227-227

 


221 EPIGENETIC DYNAMICS OF PLURIPOTENCY GENES IN THE CONTEXT OF DIFFERENTIATION AND NUCLEAR REPROGRAMMING DETERMINED BY Q2ChIP, A QUICK AND QUANTITATIVE CHROMATIN IMMUNOPRECIPITATION TECHNIQUE APPLICABLE TO SMALL CELL SAMPLES  

J. A. Dahl, C. K. Taranger and P. Collas

pp. 227-228

 


222 LOSS OF IMPRINTS OF PARTHENOGENETIC EMBRYONIC STEM CELLS IN MURINE CHIMERAS 

T. Horii, M. Kimura, S. Morita, Y. Nagao and I. Hatada

pp. 228-228

 


223 COMPARISON OF NEURAL DIFFERENTIATION BETWEEN RAT AND CAT MESENCHYMAL STEM CELLS UNDER CHALLENGE BY THE SAME NEUROINDUCTION AGENT 

G. Z. Jin, X. F. Yu, S. J. Cho, G. Y. Park, Y. J. Lee, X. J. Yin and I. K. Kong

pp. 228-228

 


224 DERIVATION AND IMMUNOLOGICAL CHARACTERISTICS OF PORCINE EMBRYONIC STEM-LIKE CELLS 

J.-E. Kim, H.-Y. Son, H.-S. Kim, E.-G. Lee and C.-K. Lee

pp. 229-229

 


225 TERATOMA FORMATION BY BOVINE EMBRYOS 

M. L. Lim, I. Vassiliev and P. J. Verma

pp. 229-229

 


226 CELL LINE-DEPENDENT GENE EXPRESSION PROFILES IN MOUSE EMBRYONIC STEM CELLS  

S. Mamo, J. Kobolak, S. Becker, M. Horsch, J. Beckers and A. Dinnyes

pp. 229-230

 


227 DIFFERENTIATION OF HEPATOCYTE-LIKE CELLS FROM MOUSE EMBRYONIC STEM CELLS IN A MONOLAYER CULTURE SYSTEM  

T. Mitani, T. Nagai, T. Masutani, H. Kato, K. Saeki, K. Matsumoto, Y. Hosoi and A. Iritani

pp. 230-230

 


228 ESTABLISHMENT OF EMBRYONIC STEM-LIKE CELL LINES IN RABBITS 

Y.-W. Ou, K.-H. Lee, L.-R. Chen, P.-C. Tang, H.-F. Guu, M.-J. Chen and J.-C. Ju

pp. 230-231

 


229 DERIVATION OF PRESUMPTIVE GONOCYTES IN VITRO FROM PRIMATE EMBRYONIC STEM CELLS 

T. Teramura, N. Kawata, T. Takehara, N. Fujinami, M. Takenoshita, K. Matsumoto, K. Saeki, N. Sagawa, Y. Hosoi and A. Iritani

pp. 231-231

 


230 GENERATION OF PUTATIVE RABBIT EMBRYONIC STEM CELLS 

S. Wang, X. Tang, Y. Niu, H. Chen, T. Li, T. Tan and W. Ji

pp. 231-232

 


231 HISTOCOMPATIBLE PARTHENOGENETIC EMBRYONIC STEM CELLS 

A. Yabuuchi, K. Kitai, A. Takeuchi, P. Lerou, K. Ng, J. West and G. Daley

pp. 232-232

 


232 SERUM-FREE DERIVATION OF CAT EMBRYONIC STEM-LIKE CELLS DERIVED FROM IN VIVO-PRODUCED BLASTOCYSTS ON CAT EMBRYONIC FIBROBLAST FEEDERS  

X. F. Yu, X. J. Jin, D. S. Choi, D. K. Jung, B. H. Choi, S. G. Cho and I. K. Kong

pp. 232-232

 


233 NON-CROSS-CONTAMINATION OF BOVINE EMBRYOS WITH MICROBES USING THE OPS VITRIFICATION SYSTEM  

A. Bielanski and A. Hanniman

pp. 232-233

 


234 QUANTITATION OF BOVINE VIRAL DIARRHEA VIRUS ASSOCIATED WITH SINGLE ZONA PELLUCIDA-INTACT IN VIVO-DERIVED BOVINE EMBRYOS 

J. A. Gard, M. D. Givens, P. K. Galik, D. A. Stringfellow, K. P. Riddell, S. E. Marley and B. K. Whitlock

pp. 233-233

 


235 MORPHOMETRIC DETERMINATION OF ENDOMETRIAL LEUKOCYTE MIGRATION DURING DIFFERENT STAGES OF THE EQUINE ESTROUS CYCLE  

D. Gerber and D. H. Volkmann

pp. 233-234

 


236 NORMAL REPRODUCTIVE CAPACITY OF HEIFERS RESULTING FROM TRANSFER OF IN VITRO-FERTILIZED EMBRYOS CULTURED WITH AN ANTIVIRAL COMPOUND 

M. D. Givens, D. A. Stringfellow, K. P. Riddell and P. K. Galik

pp. 234-234

 


237 POTASSIUM CONCENTRATION AND mRNA LEVELS OF POTASSIUM CHANNELS DECREASED IN CYSTIC OVARIAN FOLLICLE FLUID  

D. Kang, C. Choe, E. S. Kim, H. Y. Yang, C. G. Hur, E. J. Kim and J. Han

pp. 234-234

 


238 EFFICACY OF RECOMBINANT TRYPSIN AGAINST BOVINE HERPESVIRUS-1 ASSOCIATED WITH IN VITRO-DERIVED PORCINE EMBRYOS 

M. S. D. Marley, M. D. Givens, P. K. Galik, K. P. Riddell and D. A. Stringfellow

pp. 234-235

 


239 EMBRYOTOXICITY ASSAY FOR ANTIVIRAL COMPOUND BPIP (5-[(4-BROMOPHENYL)METHYL]-2-PHENYL-5H-IMIDAZO[4,5-c]PYRIDINE) IN BOVINE IN VITRO-PRODUCED EMBRYOS 

J. Mestach, J. Paeshuyse, J. Neyts, H. J. Nauwynck, D. Maes and A. Van Soom

pp. 235-236

 


240 RECOMBINANT BOVINE TRYPSIN MADE IN MAIZE INACTIVATES BOVINE HERPES VIRUS-1 ADSORBED TO THE BOVINE ZONA PELLUCIDA 

G. E. Seidel, Jr, M. L. Turk, P. W. Gordy and R. A. Bowen

pp. 236-236

 


241 TIME POINTS FOR IN VITRO MATURATION OF LION (PANTHERA LEO) OOCYTES  

S. Adamiak and P. Bartels

pp. 236-237

 


242 EFFECT OF SPERMATOZOA HEAD MORPHOMETRIC DIMENSIONS ON FREEZABILITY IN BROWN BEAR (URSUS ARCTOS) 

L. Anel, V. Garcia-Macias, F. Martinez-Pastor, M. Alvarez, S. Borragan, J. Bernardo, S. Alves, C. Chamorro and P. Paz

pp. 237-237

 


243 IN VITRO PRODUCTION OF LLAMA EMBRYOS BY IVF OR ICSI 

P. A. Conde, C. Herrera, M. G. Chaves, S. M. Giuliano, A. Director, V. L. Trasorras, M. Pinto, M. I. Sarchi, D. Stivale, B. Rutter, A. Agüero, M. H. Miragaya and R. S. Pasqualini

pp. 237-238

 


244 DEVELOPMENT OF SUPEROVULATORY STRATEGIES IN ALPACAS 

G. Gamarra, A. Gallegos, M. Asparrin and H. W. Vivanco-Mackie

pp. 238-238

 


245 USE OF A TRIPLE STAIN (SYBR-14/PI/MC540) FOR VIABILITY AND CAPACITATION ASSESSMENT IN THAWED SEMEN FROM BROWN BEAR (URSUS ARCTOS)  

V. Garcia-Macias, F. Martinez-Pastor, M. Alvarez, P. Paz, S. Borragan, E. Anel, M. Mata, M. Nicolas and L. Anel

pp. 239-239

 


246 IN VITRO PRODUCTION OF SPRINGBOK (ANTIDORCAS MARSUPIALIS) EMBRYOS  

R. Krisher, A. Auer, K. Clark, K. Emsweller, S. Rogers, K. Thomas, F. Chatiza and P. Bartels

pp. 239-240

 


247 SUCCESSFUL ARTIFICIAL INSEMINATION IN THE CORN SNAKE (ELAPHE GUTATTA), USING FRESH AND COOLED SEMEN 

J. K. Mattson, A. T. DeVries, S. M. McGuire, J. Krebs, E. E. Louis and N. M. Loskutoff

pp. 240-240

 


248 IN VITRO PRODUCTION OF CATTLE × BUFFALO HYBRID EMBRYOS USING BOVINE OOCYTES AND AFRICAN BUFFALO (SYNCERUS CAFFER CAFFER) EPIDIDYMAL SPERM 

O. D. Owiny, D. M. Barry, M. Agaba and R. A. Godke

pp. 240-240

 


249 INTRACYTOPLASMIC SPERM INJECTION OF ELAND (TAUROTRAGUS ORYX) AND BONGO (TRAGELAPHUS EURYCERUS)ANTELOPE OOCYTES 

G. Wirtu, C. E. Pope, M. C. Gomez, A. Cole, D. L. Paccamonti and B. L. Dresser

pp. 241-241

 


250 TRANSCRIPTIONAL PROFILING OF BOVINE OOCYTES DERIVED FROM THE GROWTH AND DOMINANCE PHASES OF THE FIRST FOLLICULAR WAVE 

N. Ghanem, M. Hoelker, F. Rings, D. Jennen, E. Tholen, M. A. Sirard, K. Schellander and D. Tesfaye

pp. 241-242

 


251 FOLLICLE DEVIATION AND OVULATORY CAPACITY IN BOS INDICUS HEIFERS  

L. U. Gimenes, N. A. T. Carvalho, M. F. Sá Filho, H. Ayres, J. R. S. Torres-Júnior, A. H. Souza, G. A. Bó, C. M. Barros, E. S. Sartorelli, P. S. Baruselli and R. J. Mapletoft

pp. 242-242

 


252 THE ROLE OF INSULIN-LIKE GROWTH FACTOR 1 (IGF-1) IN DEVELOPMENT OF AN ESTROGEN-ACTIVE DOMINANT FOLLICLE DURING THE FIRST FOLLICULAR WAVE POSTPARTUM IN DAIRY COWS  

C. Kawashima, N. Sudo, C. Amaya Montoya, E. Kaneko, M. Matsui, N. Matsunaga, M. Tetsuka, T. Shimizu, K. Kida, Y.-I. Miyake and A. Miyamoto

pp. 242-243

 


253 GROWTH OF PRIMORDIAL OOCYTES FROM INFANT AND ADULT PORCINE OVARIES IN XENOGRAFTS  

M. Moniruzzaman, J. Lee and T. Miyano

pp. 243-243

 


254 DEVELOPMENTAL COMPETENCE OF MOUSE OOCYTES AFTER IN VITRO GROWTH, NUCLEAR TRANSFER, AND IN VITRO FERTILIZATION  

Y. Obata and T. Kono

pp. 243-243

 


255 EFFECT OF THE TYPE OF DIETARY FATTY ACID (α-LINOLENIC ACID OR LINOLEIC ACID) ON THE NUMBER AND THE QUALITY OF OOCYTES COLLECTED BY OVUM PICKUP AND ON IN VITRO EMBRYO PRODUCTION BY DAIRY HEIFERS  

A. A. Ponter, J. Arnault, C. Joly, K. Guelou, G. Valy, S. Ponchon, C. Gonzales, B. Grimard and P. Humblot

pp. 244-244

 


256 SWITCH IN THE EXPRESSION OF GENES INVOLVED IN LIPID METABOLISM FOR IN VIVO-MATURED BOVINE OOCYTES AND BLASTOCYSTS 

O. Algriany, P. L. A. M. Vos, M. A. Sirard and S. J. Dieleman

pp. 244-244

 


257 EXPRESSION PROFILING OF GENES CRUCIAL FOR LINEAGE DETERMINATION IN IN VITRO-DERIVED EARLY BOVINE EMBRYOS  

S. Antonini, G. Lazzari, F. Cillo, C. Galli, S. Colleoni, I. Lagutina, F. Gandolfi and T. A. L. Brevini

pp. 245-245

 


258 EXPRESSION PATTERN OF THE MATERNAL FACTOR ZYGOTE ARREST 1 (ZAR1) GENE IN OVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS  

F. Ariu, D. Bebbere, S. Succu, S. Tore, L. Bogliolo, S. Fois, G. G. Leoni and S. Ledda

pp. 245-245

 


259 DNA HYPOMETHYLATION OF FETAL FIBROBLASTS IMPROVES DEVELOPMENTAL COMPETENCY AND GENE EXPRESSION PATTERN IN PORCINE EMBRYOS FOLLOWING NUCLEAR TRANSFER  

B. Mohana Kumar, H. F. Jin, J. G. Kim, S. A. Ock, H. J. Song, Y. J. Jeong, S. Balasubramanian and G. J. Rho

pp. 246-246

 


260 COMPARISON OF HOUSEKEEPING GENE EXPRESSION IN INDIVIDUAL 8-CELL STAGE MOUSE EMBRYOS PRODUCED UNDER DIFFERENT CONDITIONS 

A. Baji Gal, S. Mamo, S. Bodo and A. Dinnyes

pp. 246-246

 


261 PHENOTYPIC CHARACTERISTICS AND TISSUE-SPECIFIC IGF2R/IGF2 EXPRESSION PARTITION BOVINE FETAL OVERGROWTH ASSOCIATED WITH IN VITRO FERTILIZATION AND SOMATIC CELL NUCLEAR TRANSFER CLONING  

D. Bebbere, S. E. Ulbrich, V. Zakhartchenko, M. Weppert, H.-D. Reichenbach, H. H. D. Meyer, S. Ledda, E. Wolf and S. Hiendleder

pp. 247-247

 


262 ASSESSMENT OF HSP70-1 TRANSCRIPTION LEVELS IN IMMATURE OOCYTES FROM BOS TAURUS AND BOS INDICUS COWS RAISED IN A TROPICAL CLIMATE  

L. S. A. Camargo, J. H. M Viana, R.V. Serapião, M. F. M. Guimarães, W. F. Sá and A. M. Ferreira

pp. 247-248

 


263 GENE EXPRESSION PROFILING OF IMMATURE AND IN VITRO-MATURED BOVINE OOCYTES USING AFFYMETRIX GENECHIP TECHNOLOGY  

F. Carter, T. Fair, S. Park, M. Wade, A. C. O. Evans and P. Lonergan

pp. 248-248

 


264 ANALYSIS OF DNA METHYLATION PATTERN IN PRE-IMPLANTATION-STAGE EMBRYOS DERIVED FROM NUCLEAR TRANSFER USING PORCINE EMBRYONIC GERM CELLS 

D.-H. Choi, C.-H. Park, S.-G. Lee, H.-S. Kim, H.-Y. Son and C.-K. Lee

pp. 248-249

 


265 DICER GENE EXPRESSION IN PRE-IMPLANTATION MOUSE EMBRYOS: IMPLICATION OF TRANSCRIPTION REGULATION AT THE BLASTOCYST STAGE  

X. S. Cui, X. H. Shen, X. Y. Li, J. M. Kim and N. H. Kim

pp. 249-249

 


266 EFFECT OF 2,2′,4,4′-TETRABROMODIPHENYL ETHER ON THE EXPRESSION OF CALBINDIN-d9 k IN THE UTERUS OF IMMATURE RATS 

V.-H. Dang and E.-B. Jeung

pp. 249-249

 


267 CLASSICAL AND NON-CLASSICAL MAJOR HISTOCOMPATABILITY COMPLEX CLASS I GENE EXPRESSION IN IN VITRO-DERIVED BOVINE EMBRYOS  

J. Doyle, S. A. Ellis, P. Lonergan and T. Fair

pp. 250-250

 


268 THE EFFECTS OF METFORMIN ON THE INSULIN SIGNALING PATHWAY ON PORCINE GRANULOSA LUTEIN CELLS IN A PRIMARY CULTURE SYSTEM 

M. S. Hossein, M. S. Lee and W. S. Hwang

pp. 250-250

 


269 EXPRESSION OF TANDEM-PORE DOMAIN K+ CHANNELS IN BOVINE OOCYTES AND PRE-IMPLANTATION EMBRYOS  

C. G. Hur, D. Kang, J. Y. Park, S. G. Hong and J. Han

pp. 251-251

 


270 IN VITRO MATURATION ALTERS THE EXPRESSION OF SPECIFIC PUTATIVELY IMPRINTED GENES IN BOVINE MII OOCYTES  

M. G. Katz-Jaffe, B. R. McCallie, K. Preis, G. E. Seidel and D. K. Gardner

pp. 251-251

 


271 IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES IN A 35-DAY-OLD CLONED PIG FETUS USING THE ANNEALING CONTROL PRIMER SYSTEM 

Y. G. Ko, H. J. Chung, N. Y. Lee, H. J. Chung, H. J. Park, M. J. Kim, M. S. Choi, J. S. Kim, B. C. Yang, B. S. Yang, H. H. Seong and G. S. Im

pp. 251-252

 


272 EXPRESSION OF Lef1 TRANSCRIPTION FACTOR AND ITS NOVEL ISOFORMS IN PRE-IMPLANTATION EMBRYOS 

A. Meece, S. He and C. Keefer

pp. 252-252

 


273 EXPRESSION PATTERN OF CONNEXIN 43 (Cx43) AND POLY-(A) POLYMERASE (PAP) GENES IN BUFFALO (BUBALUS BUBALIS) EMBRYOS PRODUCED IN VITRO 

D. A. Mishra, V. Chandra, G. S. Kumar and G. T. Sharma

pp. 252-252

 


274 LOCALIZATION OF MACROPHAGE COLONY STIMULATING FACTOR (M-CSF) GENE IN THE ENDOMETRIUM OF JAPANESE BLACK COW  

K. Oshima, K. Yoshihara, T. Kojima, M. Komatsu and N. Yamamoto

pp. 253-253

 


275 EXPRESSION OF THE ACYL-COA SYNTHETASE 4 IN THE PERI-IMPLANTATION MOUSE UTERUS 

H. Y. Park, S. M. Lee, H.-M. Kim, S.-K. Yoon, S. J. Moon and M.-J. Kang

pp. 253-253

 


276 PRODUCTION OF TRANSGENIC MINI-PIG CELL LINES EXPRESSING HUMAN CYTOMEGALOVIRUS US2 

K. W. Park, J. Y. Yoo, K. M. Choi, S. P. Hong, G. S. Han, E. J. Kim, S. H. Kim, T. S. Kim, S. Y. Park, B. S. Yang, G. S. Im, K. S. Jun, K. N. Heo and J. G. Seol

pp. 254-254

 


277 PRELIMINARY STUDY OF THE DIFFERENCE IN THE GENOME-WIDE METHYLATION PROFILES BETWEEN GERM CELLS AND SOMATIC CELLS OF BULLS 

N. Phutikanit, J. Suwimonteerabutr, D. Harrison, M. J. D'Occhio, B. J. Carroll and M. Techakumphu

pp. 254-254

 


278 GENE EXPRESSION PROFILE OF OVINE OOCYTES AND CUMULUS CELLS WITH REFERENCE TO PREMATING NUTRITION  

P. Pocar, L. F. Pisani, S. Antonini, V. Tosetti, T. A. L. Brevini, F. Gandolfi and S. M. Rhind

pp. 254-255

 


279 DIFFERENCES IN TRANSCRIPT ABUNDANCE BETWEEN MALE AND FEMALE BOVINE BLASTOCYSTS PRODUCED IN VITRO USING SEXED SEMEN 

D. Rizos, P. Lonergan, D. Rath, J. De la Fuente, M. Wade, I. Parrilla-Riera and A. Gutierrez-Adan

pp. 255-255

 


280 EXPRESSION OF HSP70.1 GENE IN IN VITRO-PRODUCED BOVINE EMBRYOS CULTURED IN CR2 MEDIUM SUPPLEMENTED WITH KNOCKOUT™SR 

R. V. Serapião, L. S. de Almeida Camargo, A. de Almeida Ramos, I. de Moura Folhadella, J. Polisseni, E. Lopes, J. H. Moreira Viana, A. de Moraes Ferreira, M. F. M. Guimarães, W. Ferreira de Sá and F. A. Fonseca

pp. 255-256

 


281 EXPRESSION PROFILE AND KNOCKDOWN ANALYSIS OF A FUNCTIONALLY UNKNOWN DD2-2 GENE IN MOUSE PRE-IMPLANTATION EMBRYOS  

S. Shin, K. Matsumoto, T. Amano, K. Saeki, Y. Hosoi and A. Iritani

pp. 256-256

 


282 GENE EXPRESSION PATTERNS AND QUALITY OF BOVINE EMBRYOS PRODUCED UNDER DIFFERENT OXYGEN CONCENTRATIONS  

W. J. Son, M. K. B. , Y. J. Jeong, S. Balasubramanian, S. Y. Choe and G. J. Rho

pp. 256-257

 


283 TRANSCRIPTIONAL ANALYSIS OF BIOPSIES DERIVED FROM IN VITRO-PRODUCED BOVINE BLASTOCYSTS IN RELATION TO PREGNANCY SUCCESS AFTER TRANSFER TO RECIPIENTS 

D. Tesfaye, A. El-Sayed, M. Hoelker, F. Rings, D. Jennen, E. Tholen, M. A. Sirard and K. Schellander

pp. 257-257

 


284 IDENTIFICATION AND CHARACTERIZATION OF THE 5′-FLANKING REGION OF THREE MOUSE MATERNAL GENES (HISTONE H1OO, NUCLEOPLASMIN 2, AND ZYGOTE ARREST 1): TRANSCRIPTIONAL ACTIVITY IN MOUSE OOCYTES 

K. Tsunemoto, K. Matsumoto, M. Anzai, M. Hayakumo, T. Amano, T. Mitani, H. Kato, Y. Hosoi, K. Saeki and A. Iritani

pp. 257-258

 


285 TRANSCRIPT LEVEL OF Hsp70 AND IGF2R GENES IN BOVINE INDIVIDUAL BLASTOCYSTS DERIVED FROM OOCYTES MATURED WITH FBS OR FATTY ACID-FREE BSA 

E. Warzych, E. Pers, A. Buszka, T. Strabel and D. Lechniak

pp. 258-258

 


286 EFFECTS OF PORCINE FOLLICULAR FLUID DURING IN VITRO MATURATION OF OOCYTES ON IN VITRO FERTILIZATION AND EMBRYONIC DEVELOPMENT  

B. Agung, T. Otoi, D. Fuchimoto, S. Senbon, A. Onishi and T. Nagai

pp. 258-259

 


287 RESCUE AND IN VITRO MATURATION OF FOLLICULAR OOCYTES IN CHINCHILLA LANIGER 

G. Aiudi, M. Cinone, F. Maritato, A. De Sandro Salvati and M. E. Dell'Aquila

pp. 259-259

 


288 KINETICS OF FERTILIZATION, DEVELOPMENT, AND SEX RATIO OF IN VITRO-PRODUCED BOVINE EMBRYOS OBTAINED WITH FOUR DIFFERENT BULLS  

M. Alomar, H. Tasiaux, S. Remacle, F. George, D. Paul and I. Donnay

pp. 259-260

 


289 EFFECT OF NEURAMINIDASE AND MANNOSIDASE IN THE BOVINE IN VITRO FERTILIZATION 

S. Canovas, M. Aviles and P. Coy

pp. 260-260

 


290 ENZYMATIC ACTIVITY LEVEL OF SOME GLYCOSIDASES IN BOVINE OVIDUCTAL FLUID AT DIFFERENT STAGES OF THE ESTROUS CYCLE 

C. Carrasco, R. Romar, J. Marcos, M. Aviles and P. Coy

pp. 260-261

 


291 EFFECTS OF ANTIOXIDANT AND CULTURE MEDIUM ON THE DEVELOPMENT OF PORCINE IN VITRO-MATURED–IN VITRO-FERTILIZED EMBRYOS  

C. Choe, D.-S. Son, S.-H. Choi, S.-R. Cho, H.-J. Kim, S.-H. Yeon and M.-H. Han

pp. 261-261

 


292 OOCYTES FROM FOLLICLES OF DIFFERENT DIAMETERS: EMBRYO IN VITRO DEVELOPMENTAL POTENTIAL AND QUALITY  

T. H. C. de Bem, K. R. L. Schwarz, L. G. Mesquita, P. R. Adona and C. L. V. Leal

pp. 261-262

 


293 EFFECT OF GUAIAZULENE ON IN VITRO BOVINE EMBRYO PRODUCTION  

I. Dimitriadis, E. A. Rekka, E. Vainas, G. S. Amiridis and C. A. Rekkas

pp. 262-262

 


294 IN VITRO DEVELOPMENT OF RAT OOCYTES FOLLOWING MICROINJECTION OF A CUMULUS CELL INTO THE OOPLASM AND CHEMICAL ACTIVATION 

W. Fujii and H. Funahashi

pp. 262-262

 


295 INFLUENCE OF FERTILIZATION MEDIUM ON THE INCIDENCE OF POLYSPERMY IN THE PIG 

C. Galli, G. Crotti, P. Turini, I. Lagutina and G. Lazzari

pp. 263-263

 


296 EFFECT OF FROZEN SEMEN WITH OSTEOPONTIN ON IN VITRO BOVINE FERTILIZATION AND EMBRYO DEVELOPMENT  

R. F. Gonçalves, R. P. Bertolla, I. Eder, D. A. Chapman and G. J. Killian

pp. 263-264

 


297 ADDITION OF GLUTATHIONE TO THAWING MEDIUM FOR BULL SPERMATOZOA IMPROVES THE IN VITRO EMBRYO PRODUCTION 

L. Grullón, J. C. Gardó, F. García-Vázquez and J. Gadea

pp. 264-264

 


298 EFFECTS OF TRICHOSTATIN A DURING IN VITRO FERTILIZATION OF BOVINE OOCYTES ON SUBSEQUENT DEVELOPMENT, CELL NUMBER, AND ALLOCATION OF RESULTING EMBRYOS  

S. Ikeda, K. Saeki, A. Tatemizo, D. Iwamoto, A. Kasamatsu, S. Taniguchi, Y. Hoshino, T. Amano, K. Matsumoto, Y. Hosoi and A. Iritani

pp. 164-165

 


299 LEPTIN ACCELERATES PRONUCLEAR FORMATION FOLLOWING INTRACYTOPLASMIC SPERM INJECTION OF PORCINE OOCYTES: POSSIBLE ROLE OF MITOGEN-ACTIVATED PROTEIN KINASE INACTIVATION  

Y. X. Jin, X. S. Cui, W. K. Chang, T. Kim and N. H. Kim

pp. 265-265

 


300 BLASTOCYST PRODUCTION BY IN VITRO MATURATION AND DEVELOPMENT OF PORCINE OOCYTES IN DEFINED MEDIA FOLLOWING INTRACYTOPLASMIC SPERM INJECTION 

Y. Kobayashi and M. Fukui

pp. 265-265

 


301 EFFECT OF CO-CULTURE WITH DIFFERENT STAGE EMBRYOS ON DEVELOPMENT OF ALGINATE-ENCAPSULATED SMALL NUMBER BOVINE EMBRYOS  

S. Kobayashi, M. Sakatani, Y. Inaba, S. Kobayashi, K. Imai and M. Takahashi

pp. 266-266

 


302 EFFECT OF MONOSACCHARIDES ON SPERM ABILITY TO BIND TO OVIDUCTAL EPITHELIAL CELLS  

Y. Kon, H. Iwata, H. Shiono, K. Matsubara, T. Kuwayama and Y. Monji

pp. 266-266

 


303 FERTILIZATION-RELATED PARAMETERS OF FROZEN–THAWED SPERMATOZOA FROM SUBFERTILE JAPANESE BLACK CATTLE  

K. Kuroda, M. Fukushima, M. Miyake and H. Harayama

pp. 266-267

 


304 SHORT-PERIOD SPERM–OOCYTE INCUBATION AND FERTILIZATION MEDIA EFFECTS ON THE IN VITRO PRODUCTION OF SWINE EMBRYOS 

M. G. Marques, A. B. Nascimento, L. F. Martins, F. R. O. Barros, M. D. Goissis, M. E. O. D'Avila Assumpção and J. A. Visintin

pp. 267-267

 


305 INCREASED MONOSPERMIC PENETRATION OF PORCINE OOCYTES USING A VISCOUS IN VITRO FERTILIZATION SYSTEM 

H. Men, W. Si and J. K. Critser

pp. 268-268

 


306 IN VITRO PENETRATION OF SWINE OOCYTES MATURED IN TCM-199 WITH ADDED DIBUTYRYL CYCLIC ADENOSINE MONOPHOSPHATE AND FOLLICULAR FLUID  

A. B. Nascimento, M. G. Marques, A. R. de S. Coutinho, M. N. Tavares, M. E. O. D'Avila Assumpção, J. A. Visintin, L. Che and V. Bordignon

pp. 268-269

 


307 ADDITION OF HIGH CONCENTRATIONS OF HEPARIN DURING IN VITRO FERTILIZATION IMPROVES PERCENTAGES OF CLEAVAGE AND BLASTOCYST FORMATION WHEN BOVINE OOCYTES ARE INSEMINATED IN VITRO WITH SEX-SORTED SPERM 

T. L. Nedambale, J. Xu, S. A. Chaubal, X. C. Tian, X. Yang and F. Du

pp. 269-269

 


308 EFFECT OF GONADOTROPIN TREATMENT ON OOCYTE COLLECTION AND IN VITRO FERTILIZATION IN THE BAN MINIPIG  

B. X. Nguyen, T. Nagai, K. Kukuchi, N. T. Uoc, M. Ozawa, N. V. Linh, N. H. Duc, D. N. Q. Thanh, N. V. Hanh and Q. X. Huu

pp. 269-270

 


309 DIFFERENCES IN PRONUCLEUS FORMATION RATES BETWEEN BULLS IN RESPONSE TO GAMMA IRRADIATION OF FROZEN–THAWED SEMEN  

M. M. Petersen, G. B. Boe-Hansen, A. Birck, B. Avery, M. Jensen and I. B. Bøgh

pp. 270-270

 


310 DETERMINATION OF THE PORCINE OVIDUCTAL GLYCOSIDASES DURING THE ESTROUS CYCLE 

R. Romar, C. Carrasco, J. Marcos, M. Avilés and P. Coy

pp. 270-271

 


311 EFFECT OF HEAT SHOCK DURING THE FERTILIZATION AND CULTURE PERIOD ON BOVINE EMBRYO DEVELOPMENT AND THE PROTECTIVE EFFECT OF BROWN ALGAE PHLOROTANNINS  

M. Sakatani, K. Nagayama, K. Kobayashi, K. Kobayashi, K. Morishita, M. Asakawa and M. Takahashi

pp. 271-271

 


312 EXPOSURE OF SPERMATOZOA TO SOLUBILIZED EXTRACTS OF THE OVIDUCTAL EPITHELIUM APICAL PLASMA MEMBRANE ENHANCES FERTILIZATION IN PORCINE IN VITRO FERTILIZATION  

N. Satake, A. K. Alhaider, W. V. Holt and P. F. Watson

pp. 272-272

 


313 IMPROVEMENT OF FERTILIZATION IN VITRO BY PRE-INCUBATION OF FROZEN–THAWED RAT SPERMATOZOA WITH CAFFEINE-SUPPLEMENTED MEDIUM  

Y. Seita, S. Sugio, D. Sano, Y. Okuda and N. Kashiwazaki

pp. 272-272

 


314 OPTIMIZING TREATMENT FOR IVF WHEN USING HEAVILY BACTERIAL CONTAMINATED SEMEN  

E. M. Senatore, R. Frontoso, D. Pirazzi, M. E. Mannino, G. Iovane and G. A. Presicce

pp. 272-273

 


315 SELECTION OF BOVINE OOCYTES BY BRILLIANT CRESYL BLUE BEFORE IN VITRO MATURATION IMPROVES BLASTOCYST DEVELOPMENT 

A. Sugulle, S. Katakawa, S. Yamamoto, S. Oomori, I. Itou, O. Dochi and H. Koyama

pp. 273-273

 


316 DEVELOPMENTAL COMPETENCE OF OOCYTES SELECTED BY THE BRILLIANT CRESYL BLUE STAINING IN PREPUBERTAL AND ADULT CATTLE  

M. Tagawa, S. Matoba, M. Okada, K. Metoki and K. Imai

pp. 273-274

 


317 ANTI-HYALURONIDASE ACTION OF ELLAGIC ACID EFFECTIVELY PREVENTS POLYSPERMY THROUGH SUPPRESSION OF THE ACROSOME REACTION INDUCED BY THE SPERM–ZONA INTERACTION DURING PORCINE IN VITRO FERTILIZATION  

I. Tokeshi, H. Tatemoto, N. Muto, T. Yoshimoto, S. Nakamura and T. Nakada

pp. 274-274

 


318 EFFECT OF MATERNAL HEAT STRESS ON OOCYTE QUALITY AND IN VITRO COMPETENCE IN BOS INDICUS CATTLE  

J. R. S. Torres Júnior, M. F. A. Pires, W. F. Sá, A. M. Ferreira, J. H. M. Viana, L. S. A. Camargo, A. A. Ramos, I. M. Folhadella, J. Polisseni, C. Freitas, C. A. A. Clemente, M. F. Sá Filho, C. M. Martins, G. A. Bó and P. S. Baruselli

pp. 274-275

 


319 PROTEIN GENE PRODUCT 9.5 REGULATES SPERM PENETRATION DURING PORCINE FERTILIZATION IN VITRO  

Y. J. Yi, G. Manandhar, M. Sutovsky, C. S. Park and P. Sutovsky

pp. 275-275

 


320 EFFECT OF OXYGEN TENSION AND FOLLICLE CELLS DURING IN VITRO CULTURE OF PORCINE OOCYTES IN FOLLICULAR FLUID ON THEIR MATURATION AND FERTILIZATION 

P. Yongnan, F. Daiichiro, S. Shoichiro, O. Akira, S. Eime and T. Nagai

pp. 275-276

 


321 PREGNANCIES ESTABLISHED FOLLOWING TRANSFER OF IN VITRO-PRODUCED FRESH AND FROZEN–THAWED BUFFALO (BUBALUS BUBALIS) EMBRYOS 

X. Zhang, X. Liang, B. Yang, M. Chen, F. Huang, C. Pang, G. Qin, E. M. Senatore and G. A. Presicce

pp. 276-276

 


322 EFFECT OF SODIUM NITROPRUSSIDE ON BUFFALO SPERM CAPACITATION IN VITRO  

L. Boccia, L. Attanasio, A. De Rosa, G. Pellerano, R. Di Palo and B. Gasparrini

pp. 276-277

 


323 EXPRESSION OF PSP-I AND PSP-II IN THE REPRODUCTIVE TRACT OF THE BOAR BY IMMUNOHISTOCHEMISTRY, WESTERN BLOTTING, AND RT-PCR 

E. M. Garcia, J. M. Vazquez, I. Parrilla, J. J. Calvete, L. Sanz, E. A. Martinez, J. Roca and H. Rordríguez-Martínez

pp. 277-277

 


324 EFFECT OF AGING ON AMOUNTS OF DNA METHYLTRANSFERASE mRNA IN MOUSE SPERMATOZOA  

H. Kato, T. Koda, M. Kishimoto, T. Mitani, K. Matsumoto, K. Saeki, Y. Hosoi and A. Iritani

pp. 277-278

 


325 PROLONGING THE LIFE OF RAM SPERMATOZOA IN VITRO USING OVIDUCTAL EPITHELIAL CELLS  

R. E. Lloyd, E. Badia, P. F. Watson and W. V. Holt

pp. 278-278

 


326 COMPARING CHANGES IN MEMBRANE LIPID ORDER IN EPIDIDYMAL AND EJACULATED BOAR SPERMATOZOA 

C. Matas, F. Garcia-Vazquez,, M. Sansegundo, S. Ruiz and J. Gadea

pp. 277-278

 


327 IDENTIFICATION OF OSTEOPONTIN IN WATER BUFFALO SEMEN 

M. E. Pero, G. J. Killian, P. Lombardi, L. Zicarelli, L. Avallone and B. Gasparrini

pp. 279-279

 


328 FLUORESCENT PROBE ASSESSMENT OF POST-THAW QUALITY OF RAM SPERM TREATED WITH SEMINAL PLASMA 

M. Rovegno, W. B. Feitosa, A. C. Brandão, A. B. Nascimento, M. A. Peres, J. A. Visintin and M. E. O. A. Assumpção

pp. 279-280

 


329 COMPARING CHANGES IN MOTION PARAMETERS IN EPIDIDYMAL AND EJACULATED BOAR SPERMATOZOA UNDER THREE DIFFERENT TREATMENTS  

M. Sansegundo, J. C. Gardon, F. Garcia-Vazquez, J. Gadea and C. Matas

pp. 280-280

 


330 CORRELATION BETWEEN TWO APOPTOTIC MARKERS IN FRESH BOAR SPERMATOZOA  

M. Trzcinska, M. Bryla and Z. Smorag

pp. 280-281

 


331 DIRECT EFFECTS OF ERGOT ALKALOIDS ON BOVINE SPERM MOTILITY IN VITRO  

H. Wang, Z. Johnson, R. Rorie and C. Rosenkrans, Jr

pp. 281-281

 


332 EFFECT OF DIFFERENT PIG OOCYTE ACTIVATION PROTOCOLS ON EMBRYO DEVELOPMENT IN SOF AND NCSU-23 

I. Lagutina, G. Lazzari and C. Galli

pp. 281-282

 


333 DYNAMICS OF MITOGEN-ACTIVATED PROTEIN KINASE ACTIVITY IN PIG OOCYTES FOLLOWING PARTHENOGENETIC ACTIVATION BY DIFFERENT METHODS  

L. Nanassy, K. Lee, A. Javor and Z. Machaty

pp. 282-282

 


334 DNA SYNTHESIS DURING THE FIRST CELL CYCLE OF PORCINE OOCYTES FOLLOWING DIFFERENT ACTIVATION TREATMENTS  

S.-A. Ock, D. Kang and J. Han

pp. 282-283

 


335 EFFECT OF VITAMIN E ON THE DEVELOPMENT OF BOVINE OOCYTES AFTER CHEMICAL ACTIVATION  

J. I. Park, Y. Jang and E. S. Lee

pp. 283-283

 


336 DEVELOPMENT OF PORCINE NUCLEAR TRANSFER EMBRYOS PRODUCED USING SPERM CYTOSOLIC FACTOR (SCF) AT FUSION-ACTIVATION  

J. H. Shim, I. S. Hwang, H. J. Moon, M. R. Park, D. H. Kim, B. C. Yang, Y. K. Ko, S. W. Kim, B. S. Yang, D. I. Jin and G. S. Im

pp. 283-283

 


337 GENE EXPRESSION IN BOVINE OOCYTES AFTER MEIOSIS BLOCK WITH BUTYROLACTONE I  

P. R. Adona, F. H. Biase, T. H. C. de Bem and C. L. V. Leal

pp. 284-284

 


338 INFLUENCE OF OOCYTE DIAMETER AND MORPHOLOGY ON APOPTOSIS AND BLASTOCYST DEVELOPMENT IN PREPUBERTAL GOAT 

B. Anguita, M. T. Paramio, A. R. Jimenez-Macedo, R. Romaguera, R. Morato, T. Mogas and D. Izquierdo

pp. 284-285

 


339 OXYGEN CONCENTRATION DURING IN VITRO MATURATION OF MURINE OOCYTES INFLUENCES SUBSEQUENT FETAL AND PLACENTAL OUTCOMES  

K. M. Banwell, M. Lane, D. L. Russell, K. L. Kind and J. G. Thompson

pp. 285-285

 


340 EFFECT OF GLUCOSE ON THE MORPHOLOGY OF GERMINAL VESICLE AND MEIOTIC PROGRESSION OF PORCINE OOCYTES IN A CHEMICALLY DEFINED MEDIUM  

H. Funahashi and T. Koike

pp. 285-286

 


341 IN VITRO MATURATION OF GOAT FOLLICULAR OOCYTES SUBSEQUENT TO THEIR CRYOPRESERVATION BY VITRIFICATION  

N. Garg and G. N. Purohit

pp. 286-286

 


342 DISTINCT EFFECTS OF FOLLICULAR FLUID ON CUMULUS CELL APOPTOSIS AND PORCINE OOCYTE DEVELOPMENTAL COMPETENCE  

C. G. Grupen, T. S. Hussein, S. J. Schulz and D. T. Armstrong

pp. 286-287

 


343 DISTRIBUTION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND IN VITRO MATURATION OF PORCINE OOCYTE–CUMULUS COMPLEXES FROM SMALL AND MEDIUM FOLLICLES  

Y. Ishida and H. Funahashi

pp. 287-287

 


344 FOLLICULAR FLUIDS COLLECTED FROM LARGE FOLLICLES IMPROVED THE PROGRESSION OF IN VITRO NUCLEAR MATURATION OF PIG OOCYTES  

M. Ito, M. Kitagawa, H. Iwata, T. Kuwayama and Y. Monji

pp. 287-288

 


345 EFFECT OF FOLLICLE STIMULATING HORMONE AND HUMAN CHORIONIC GONADOTROPHIN ON THE IN VITRO MATURATION OF CANINE OOCYTES  

M.-K. Kim, H.-J. Oh, Y. H. Fibrianto, G. Jang, H.-J. Kim, S.-G. Hong, J.-E. Park, S.-K. Kang, B.-C. Lee and D.-Y. Kim

pp. 288-288

 


346 BOVINE EMBRYO DEVELOPMENT AFTER IVM/IVF/IVC OF OOCYTES STORED FOR 22 H IN VARIOUS MEDIA  

C. Kubota, T. Kojima, T. Nagai, X. Tian and X. Yang

pp. 288-288

 


347 EFFECTS OF NEWBORN CANINE SERUM ON IN VITRO MATURATION OF CANINE OOCYTES  

H. S. Lee, D. S. Choi, D. G. Jung, I. H. Bae, S. S. Lee, X. Y. Yin and I. K. Kong

pp. 289-289

 


348 INACTIVATION OF MAPK AFFECTS CENTROSOME ASSEMBLY, BUT NOT ACTIN FILAMENT ASSEMBLY, IN MOUSE OOCYTES MATURING IN VITRO  

S. E. Lee, J. H. Kim, Y. K. Ji and N. H. Kim

pp. 289-289

 


349 DEVELOPMENTAL COMPETENCE AFTER IVF OF CUMULUS-ENCLOSED OOCYTES FROM A ICR MOUSE STRAIN CULTURED WITH A LOW CONCENTRATION OF HYPOXANTHINE DURING IVM  

R. Oishi, K. Ono, M. Teranishi, H. Imai and M. Yamada

pp. 289-290

 


350 EFFECT OF INDIRECT MAINTENANCE OF INTRACELLULAR cAMP IN CUMULUS–OOCYTE COMPLEXES USING 3-ISOBUTYL-1-METHYLXANTHINE ON GAP JUNCTIONAL COMMUNICATIONS AND OOCYTE MEIOTIC MATURATION  

M. Ozawa, T. Nagai, T. Somfai, M. Nakai, N. Maedomari, M. Fahrudin, N. W. K. Karja, H. Kaneko, J. Noguchi, K. Ohnuma and K. Kikuchi

pp. 290-290

 


351 EFFECTS OF FETAL CALF SERUM, GROWTH FACTOR, AND HORMONE SUPPLEMENTATION DURING IN VITRO MATURATION ON PARTHENOGENETIC ACTIVATION AND EMBRYO DEVELOPMENT OF FOLLICULAR RABBIT OOCYTES 

Z. Polgar, T. Somfai, V. Angeli, X. H. Tang, W. Ji and A. Dinnyes

pp. 290-291

 


352 EFFECTS OF FOLLICLE SIZE AND STAGE OF MATURATION ON mRNA EXPRESSION IN BOVINE IN VITRO-MATURED OOCYTES  

S. Racedo, D. Herrmann, C. Wrenzycki, D. Salamone and H. Niemann

pp. 291-291

 


353 EFFECTS OF CULTURE DURATION AND CUMULUS CELL REMOVAL ON CANINE OOCYTE IN VITRO MATURATION AND FERTILIZATION USING VASAL SPERM  

M. Ridha-Albarzanchi, J. Liu, M. Kjelland and D. Kraemer

pp. 292-292

 


354 IN VITRO DEVELOPMENT OF BOVINE EMBRYOS AFTER NITRIC OXIDE INHIBITION  

K. R. L. Schwarz, T. H. C. de Bem, T. T. Zampieri, P. R. Adona and C. L. V. Leal

pp. 292-292

 


355 DEVELOPMENTAL COMPETENCE OF IMMATURE BUBALINE CUMULUS–OOCYTE COMPLEXES VITRIFIED BY THE OPEN PULLED STRAW AND CONVENTIONAL STRAW METHODS  

A. Sharma and G. N. Purohit

pp. 293-293

 


356 IMPROVED ENUCLEATION EFFICIENCY FOR PIG SOMATIC CELL NUCLEAR TRANSFER BY DENUDING OOCYTES AT 30 HOURS OF IN VITRO MATURATION  

K. Song, J. Park and E. Lee

pp. 293-294

 


357 CALCIUM RELEASE INDUCED BY THIMEROSAL AND INOSITOL 1,4,5-TRIPHOSPHATE IN HEAT-SHOCKED PORCINE OOCYTES 

J.-K. Tseng, P.-C. Tang and J.-C. Ju

pp. 294-294

 


358 MORPHOLOGICAL CHANGES IN VITRIFIED WATER BUFFALO CUMULUS–OOCYTE COMPLEXES AND THEIR IN VITRO NUCLEAR MATURATION 

R. C. S. Yadav, A. Sharma and G. N. Purohit

pp. 294-294

 


359 THE EFFICIENT MAINTENANCE OF OXYGEN CONSUMPTION BY PORCINE OOCYTES RELATES TO THE HIGH COMPETENCE OF OOCYTE MATURATION 

M. Yokoo, T. Ito-Sasaki, H. Shiku, T. Matsue, S. Aoyagi, H. Hoshi and H. Abe

pp. 295-295

 


360 EVALUATION OF QUALITY OF IN VITRO-MATURED BOVINE OOCYTES BY SEDIMENTATION WITH PERCOLL 

K. Yotsushima, M. Shimizu, H. Kon and Y. Izaike

pp. 295-296

 


361 INFLUENCE OF SEXING PROCEDURE ON BULL SPERM CHROMATIN STRUCTURE 

M. Bochenek, T. Herjan and Z. Smorag

pp. 296-296

 


362 SEX RATIO OF IN VITRO-PRODUCED BLASTOCYSTS OBTAINED USING FROZEN SEMEN FROM A CLONED BULL OR ITS ORIGINAL CELL DONOR  

Y. Heyman, B. LeGuienne, C. Audouard, F. Charreaux, P. Humblot and X. Vignon

pp. 296-297

 


363 FACTORS INFLUENCING PREGNANCY RATES FOLLOWING TRANSFER OF BOVINE IN VIVO EMBRYOS BIOPSIED FOR SEX DETERMINATION  

S. Li, W. Yu, J. Fu, Y. Bai, F. Jin and B. Shangguan

pp. 297-297

 


364 AMPLIFICATION AND APPLICATION OF THE HMG BOX OF BOVINE SRY GENE FOR SEX DETERMINATION 

W. Lu, N. Rawlings and J. Zhao

pp. 297-298

 


365 COMPARISON OF IN VITRO FERTILIZING CAPACITY OF FROZEN–THAWED SEX-SORTED AND SEX-SORTED FROZEN–THAWED BULL SPERMATOZOA 

V. Malcolm, M. Marfil, M. Calvi, F. Rigali, M. Pugliese, J. Gutierrez, M. Panarace and M. Medina

pp. 298-298

 


366 SEX IDENTIFICATION OF PORCINE EMBRYOS BY PCR BASED ON THE AMELOGENIN GENE 

S. Senbon, S.-I. Suzuki, D.-I. Fuchimoto, M. Iwamoto, T. Kawarasaki and A. Onishi

pp. 298-299

 


367 FLUORESCENT IN SITU HYBRIDIZATION FOR SEX DETERMINATION AT PROGRESSIVE STAGES OF FERTILIZATION AND DEVELOPMENT IN MICE 

J. J. Whyte, E. D. Fountain and C. S. Rosenfeld

pp. 299-299

 


368 FIELD STUDIES ON THE EFFECTIVENESS OF THE YCD EMBRYO SEXING TECHNIQUE IN BOVINE  

W. Yu, S. Li, J. Fang, X. Sun, L. Cui, J. Fu, Y. Bai, Y. Fang and B. Shangguan

pp. 299-300

 


369 AMPLIFICATION OF THE SRY GENE FOR SEXING OF BUFFALO (BUBALUS BUBALIS) EMBRYOS USING NESTED MULTIPLEX PCR 

M. Zhang, Q. Fu, W. S. Qin, H. Y. Zheng, Y. Q. Lu, S. S. Lu and K. H. Lu

pp. 300-300

 


370 BILATERAL DIFFERENTIAL TESTICULAR BIOPSIES IMPROVE TESE AND ICSI OUTCOMES IN NON-OBSTRUCTIVE AND OBSTRUCTIVE AZOOSPERMIC INFERTILE PATIENTS  

S. M. Al-Ansari, M. T. Ridha-Albarzanchi, Z. A. Kanan, A. A. Al-Badri and S. K. Al-Atraqchi

pp. 300-301

 


371 INTRACYTOPLASMIC SPERM INJECTION OF EQUINE OOCYTES USING AIR-DRIED SPERM OR SPERM STORED IN A HIGH OSMOLARITY MEDIUM  

A. Alonso, M. Miragaya, L. Losinno and C. Herrera

pp. 301-301

 


372 SPERM ASTER FORMATION AND BLASTOCYST DEVELOPMENT OF IN VIVO-MATURED BOVINE OOCYTES FERTILIZED BY INTRACYTOPLASMIC SPERM INJECTION  

T. Horiuchi, M. Takenaka, C. Kani, C. Emuta, Y. Ogata and T. Matushige

pp. 301-302

 


373 OOCYTE-ACTIVATING CAPACITY OF BOVINE SPERMATOGENIC CELLS AND ACTIVATION PROTOCOLS FOR INTRACYTOPLASMIC INJECTION OF BOVINE ROUND SPERMATIDS  

C. Kani, M. Takenaka, T. Muneto, M. Yamamoto and T. Horiuchi

pp. 302-302

 


374 THE EFFECTS OF POLYVINYLPYRROLIDONE ON BOVINE SPERM FUNCTION AND EMBRYO DEVELOPMENT  

Y. Kato, M. Fukushima, A. Kenmotsu, K. Chikazawa and Y. Nagao

pp. 302-303

 


375 UNEXPECTED SEVERE ABNORMALITIES IN MOUSE ROSI OFFSPRING 

P. N. Moreira, R. Fernández-González, M. Pérez-Crespo, P. Bermejo, J. D. Hourcade, R. Rey and A. Gutiérrez-Adán

pp. 303-303

 


376 EFFECT OF SPERM TREATMENT ON THE ABILITY TO ACTIVATE OOCYTES AFTER ICSI IN PIGS 

M. Nakai, N. Kashiwazaki, N. Maedomari, M. Ozawa, J. Noguchi, H. Kaneko and K. Kikuchi

pp. 303-304

 


377 SPERMATOZOA RETRIEVED FROM MALE MICE FROZEN FOR 15 YEARS CAN PRODUCE NORMAL OFFSPRING 

N. Ogonuki, K. Mochida, H. Miki, K. Inoue, T. Iwaki, K. Morozumi, R. Yanagimachi and A. Ogura

pp. 304-304

 


378 INTRACYTOPLASMIC SPERM INJECTION AND ESTABLISHMENT OF EMBRYONIC STEM CELLS IN AFRICAN GREEN MONKEYS (CERCOPITHECUS AETHIOPS)  

N. Shimozawa, H. Okada, M. Hatori, T. Yoshida and T. Sankai

pp. 304-305

 


379 EFFECT OF SUPPLEMENTARY INOSITOL 1,4,5-TRIPHOSPHATE INJECTION ON FERTILIZATION AND EMBRYO DEVELOPMENT AFTER BOVINE ICSI  

T. K. Suh and G.E. Seidel, Jr

pp. 305-305

 


380 FULL-TERM DEVELOPMENT OF MOUSE EMBRYOS PRODUCED FROM RECONSTRUCTED GIANT OOCYTES BY INTRACYTOPLASMIC SPERM HEAD INJECTION 

S. Wakayama, S. Kishigami, V. T. Nguyen, H. Ohta, T. Hikichi, E. Mizutani, Y. Sakaide, B. H. Thuy, R. Suetsugu and T. Wakayama

pp. 305-305

 


381 FERTILIZABILITY AND CHROMOSOMAL INTEGRITY OF FROZEN–THAWED BRYDE's WHALE (BALAENOPTERA EDENI) SPERMATOZOA INTRACYTOPLASMICALLY INJECTED INTO MOUSE OOCYTES 

H. Watanabe, H. Tateno, H. Kusakabe, Y. Kamiguchi, Y. Fujise, H. Ishikawa, S. Ohsumi and Y. Fukui

pp. 306-306

 


382 SYNCHRONIZING ACTIVATION TIME WITH MAXIMUM MATURATION PROMOTING FACTOR AND MITOGEN-ACTIVATED PROTEIN KINASE LEVELS DOES NOT IMPROVE BOVINE INTRACYTOPLASMIC SPERM INJECTION 

B. J. Woodward, W. E. Maalouf and K. H. S. Campbell

pp. 306-307

 


383 EFFECT OF TIME OF OVULATION INDUCTION BY GONADOTROPIN-RELEASING HORMONE OR PITUITARY LUTEINIZING HORMONE ON OVA/EMBRYO PRODUCTION IN SUPERSTIMULATED BEEF COWS INSEMINATED AT A FIXED TIME 

P. Chesta, R. Tribulo, H. Tribulo, E. Balla, P. S. Baruselli and G. A. Bo

pp. 307-307

 


384 EFFECTS OF CONDITION SCORE ON OVARIAN RESPONSE AND TRANSFERABLE EMBRYOS IN SUPERSTIMULATED COWS IN ARGENTINA 

A. Garcia Guerra, D. Rodriguez, J. Villareal, A. Albrecht and G. Brogliatti

pp. 307-308

 


385 RELATIONSHIP AMONG ESTROUS BEHAVIOR, SUPEROVULATORY RESPONSE, AND EMBRYO SEX RATIO IN SUPERSTIMULATED HOLSTEIN HEIFERS  

Y. G. Jung, A. Ideta, K. T. Lim, W. Y. Lee, H. B. Song, K. Hayama, M. Urakawa and Y. Aoyagi

pp. 308-308

 


386 EFFECTS OF TIME AND NUMBER OF INSEMINATIONS ON THE SEX RATIO OF EMBRYOS IN SUPERSTIMULATED HOLSTEIN HEIFERS 

K. T. Lim, A. Ideta, W. Y. Lee, Y. G. Jung, K. Hayama, M. Urakawa and Y. Aoyagi

pp. 308-309

 


387 EFFECT OF NUMBER OF INSEMINATIONS ON EMBRYO PRODUCTION IN SUPERSTIMULATED NELORE (BOS INDICUS) COWS SUBJECTED TO FIXED-TIME ARTIFICIAL INSEMINATION  

C. M. Martins, H. Ayres, J. R. S. Torres-Junior, A. H. Souza, G. A. Bó and P. S. Baruselli

pp. 309-309

 


388 SUPEROVULATION TREATMENT BY A SINGLE INJECTION OF FSH WITH ALUMINUM HYDROXIDE GEL IN HOLSTEIN CATTLE  

S. Matoba, M. Tagawa, H. Matsuda, H. Yoshioka, K. Kimura, H. Kadokawa, M. Yonai and K. Imai

pp. 309-310

 


389 ASSOCIATION BETWEEN WALKING ACTIVITY AND SUPEROVULATORY RESPONSE IN JAPANESE BLACK CATTLE 

M. Narita, M. Ito and Y. Tanimoto

pp. 310-310

 


390 THE EFFECT OF REDUCED FSH DOSE AND NUMBER OF TREATMENTS ON SUPEROVULATORY RESPONSE IN CIDR-TREATED KOREAN NATIVE COWS  

D. S. Son, C. Y. Choe, S. R. Cho, S. H. Choi, H. J. Kim and I. H. Kim

pp. 310-311

 


391 EFFECTS OF SEASON AND LACTATION STATUS ON EMBRYO PRODUCTION IN HOLSTEIN COWS  

J. L. M. Vasconcelos, D. T. G. Jardina, C. A. Rodrigues and V. C. Guerreiro

pp. 311-311

 


392 CHARACTERISTICS OF THE SUPEROVULATORY RESPONSE IN GYR (BOS INDICUS) CATTLE  

J. H. M. Viana, L. S. A. Camargo, L. G. B. Siqueira, E. D. Souza, C. Freitas and C. A. C. Fernandes

pp. 311-312

 


393 A STUDY OF THE USE OF SEXED SEMEN IN SUPERSTIMULATED HOLSTEIN COWS 

H. Wang, R. Q. Li, X. L. Zhang, Q. Z. Pan, J. L. Li, M. B. Zhao, Y. Z. Wang and D. Y. Chen

pp. 312-312

 


394 DEVELOPMENT OF A RAT ENDOMETRIAL SPHEROID AS A MODEL FOR THE ANALYSIS OF ENDOMETRIAL FUNCTIONS 

K. Nishimura, S. Oozono, H. Urata, P. He, T. Soh, N. Yamauchi and M.-A. Hattori

pp. 312-313

 


395 ISOLATION AND CHARACTERIZATION OF PORCINE ADIPOSE-DERIVED ADULT STEM CELLS 

A. A. Picou, S. L. Bartolotta, K. J. Williams, A. M. Giraldo, R. A. Godke and K. R. Bondioli

pp. 313-313

 


396 CHANGES OF PLASMINOGEN ACTIVATOR ACTIVITY IN OVIDUCTAL EPITHELIAL CELLS DURING THE ESTROUS CYCLE IN PIGS  

M. Y. Shin, E. H. Kwon, H. T. Cheong, B. K. Yang and C. K. Park

pp. 313-314

 


397 LONG-TERM CULTURE OF BOVINE SECONDARY FOLLICLES IN MEDIA CONTAINING BSA, FCS, PLASMA, AND FOLLICULAR FLUID  

A. Takajo, R. M. Bao, A. Hamawaki, M. Yoshikawa and T. Miyano

pp. 314-314

 


398 EFFECT OF INCUBATION PERIOD ON TRANSFECTION RATES IN BOVINE SPERMATOZOA 

M. E. O. A. Assumpção, W. B. Feitosa, M. Rovegno, M. G. Marques, A. C. Nicacio, R. Simões and J. A. Visintin

pp. 314-315

 


399 SPERMATOZOA-MEDIATED GENE TRANSFER IS INFLUENCED BY SIZE, STRUCTURE, AND CONCENTRATION OF EXOGENOUS DNA 

W. B. Feitosa, M. P. Milazzotto, E. A. L. Martins, A. M. Rocha, J. L. Avanzo, J. A. Visintin and M. E. O. A. Assumpção

pp. 315-315

 


400 PRODUCTION OF TRANSGENIC CLONED DOMESTIC CAT EMBRYOS BY LENTIVECTOR-MEDIATING TRANSGENESIS 

M. C. Gómez, R. Kutner, D. Ricks, C. E. Pope, C. Dumas, B. L. Dresser and J. Reiser

pp. 316-316

 


401 USE OF FLOW CYTOMETRY TO EVALUATE THE CAPACITY OF BOAR SPERM TO BIND TO EXOGENOUS DNA OF DIFFERENT SIZES 

F. García-Vázquez, D. Gumbao, A. Gutiérrez-Adan and J. Gadea

pp. 316-316

 


402 OOCYTE-MEDIATED GENE TRANSFER: A NOVEL APPROACH TO PRODUCE TRANSGENIC PORCINE EMBRYOS  

M. K. Gupta, S. J. Uhm, E. Y. Kim, Y. H. Jung, J. Y. Yu and H. T. Lee

pp. 317-317

 


403 IDENTIFICATION AND CHARACTERIZATION OF A NOVEL MOUSE AND HUMAN MOPT GENE CONTAINING MORN MOTIF PROTEIN IN TESTIS 

K. M. Kim, Y.-J. Choi, H. Song, K.-C. Hwang, S.-J. Kang, S.-K. Cho, M.-O. Kim and J.-H. Kim

pp. 317-317

 


404 DNA TAKE-UP BY SPERM AND IN VITRO EMBRYO DEVELOPMENT BY SPERM-MEDIATED GENE TRANSFER IN THE PIG  

T. S. Kim, Y. Cao, H. T. Cheong, B. K. Yang and C. K. Park

pp. 317-318

 


405 COMPARISON OF TARGETING PARAMETERS FOR THE HPRT GENE BETWEEN DOMESTIC AND OSSABAW SWINE 

K. Lee and Z. Machaty

pp. 318-318

 


406 EFFECTS OF SUCKLING INTENSITY ON MILK YIELD AND PIGLET GROWTH IN LACTATION-ENHANCED ALPHA-LACTALBUMIN TRANSGENIC GILTS  

K. M. Marshall, W. L. Hurley, R. D. Shanks and M. B. Wheeler

pp. 318-319

 


407 AN ATTEMPT TO GENERATE TRANSGENIC CHICKENS BY INJECTION OF EGFP OR YFP GENE-TRANSFECTED BLASTODERMAL CELLS 

Y. Matsubara, T. Tagami, H. Kagami and T. Nagai

pp. 319-319

 


408 FOREIGN GENE INTEGRATION PATTERNS IN TRANSGENIC PORCINE FETUSES PRODUCED BY ICSI-MEDIATED GENE TRANSFER  

H. Saito, M. Kurome, R. Tomii, S. Ueno, K. Hiruma, K. Hiyama, H. Matsunari, N. Nakayama, K. Hattori, K. Nakamura, H. Endo, M. Watanabe, K. Umeyama, K. Miki and H. Nagashima

pp. 319-320

 


409 EFFICIENCY OF CO-TRANSFECTION OF TRANSGENE AND Neor GENE INTO GOAT AND PIG FETAL FIBROBLASTS 

Y. M. Shin, S. M. Chang, B. C. Kim, C. S. Park and D. I. Jin

pp. 320-320

 


410 USE OF SPERMATOZOA AS VECTORS OF EXOGENOUS DNA FOR IN VITRO PRODUCTION OF BOVINE TRANSGENIC EMBRYOS 

R. Simões, M. Binelli, A. C. Nicacio, M. P. Milazzotto, W. B. Feitosa, J. A. Visintin and M. E. O. A. Assumpção

pp. 320-321

 


411 EFFECTIVE PRODUCTION OF ICSI-MEDIATED TRANSGENIC MICE: INTENSITY OF GFP EXPRESSION AS A MARKER GENE AND INCIDENCE OF APOPTOSIS IN BLASTOCYSTS  

M. Takenaka, C. Kani and T. Horiuchi

pp. 321-321

 


412 VIABILITY OF ENZYMATICALLY ISOLATED BOVINE PRIMORDIAL AND PRIMARY OVARIAN FOLLICLES COLLECTED BY THE BIOPSY PICK-UP TECHNIQUE 

J. M. J. Aerts, B. Martinez-Madrid, K. Flothmann, J. B. P. De Clercq and P. E. J. Bols

pp. 321-322

 


413 ULTRASONOGRAPHIC EVALUATION OF FETAL LIVER SIZE DURING PREGNANCY IN BOVINE CLONED FETUSES 

G. A. Jaúregui, M. Panarace, C. Garnil, A. Segovia, J. J. Lagioia, J. Gutiérrez, E. Rodriguez, M. Marfil and M. Medina

pp. 322-322

 


414 COLOR DOPPLER EVALUATION OF UMBILICAL BLOOD FLOW DURING PREGNANCY IN COWS 

M. Panarace, G. Jaúregui, C. Garnil, A. Segovia, J. Gutiérrez, J. J. Lagioia, M. Marfil, E. Rodríguez and M. Medina

pp. 323-323

 


415 USE OF COMPUTER-ASSISTED ULTRASOUND IMAGE ANALYSIS IN EMBRYO RECIPIENT SELECTION  

L. G. B. Siqueira, J . H. M. Viana, E. D. Souza, L. S. A. Camargo, J. F. Fonseca, C. A. C. Fernandes and C. A. A. Torres

pp. 323-324

 


416 EFFECTS OF THE CORPUS LUTEUM WITHIN THE OVARY ON THE FOLLICULAR DYNAMICS AFTER FOLLICULAR ASPIRATION AND ON THE DEVELOPMENTAL COMPETENCE OF ASPIRATED OOCYTES 

T. Takuma, T. Otsubo, Y. Kurokawa and T. Otoi

pp. 324-324

 


  
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