Difference in Conformation of Virginiamycin M₁ in Chloroform and Bound Form in the 50S Ribosome or Streptogramin Acetyltransferase

Jason Dang, B. Mikael Bergdahl, Frances Separovic, Robert T. C. Brownlee and Robert P. Metzger

Abstract

The conformation of virginiamycin M₁ (VM1) in chloroform, determined by high-resolution NMR experiments, differs significantly from that of the X-ray crystal structure of VM1 bound to the 50S ribosome and to the active site of a streptogramin acetyltransferase enzyme. This implies that the binding process to these entities causes a major change in VM1 conformation.

Australian Journal of Chemistry 57(5) 415 - 418 (2004) doi:10.1071/CH03326