CSIRO Publishing Books Journals About Us Shopping Cart You are here: Journals > Australian Journal of Chemistry   
Australian Journal of Chemistry
  An international journal for chemical science
 
Search
 
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
For Advertisers
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
Covers
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
General Information
Review Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

 Early Alert
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

 Connect with us
facebook   youtube

Affiliated with RACI

Royal Australian Chemical Institute
Royal Australian
Chemical Institute


 

Article << Previous     |     Next >>   Contents Vol 57(10)

Preliminary Evaluation of Dual Acidic Potassium Permanganate and Tris(2,2′-bipyridyl)ruthenium(ii) Chemiluminescence Detection for the HPLC Determination of Papaver somniferum Alkaloids

Claire E. Lenehan A, Neil W. Barnett A C, Simon W. Lewis A, Kevin M. Essery B

A Centre for Chiral and Molecular Technologies, School of Biological and Chemical Sciences, Deakin University, Geelong VIC 3217, Australia.
B GlaxoSmithKline, Port Fairy VIC 3284, Australia.
C Author to whom correspondence should be addressed (e-mail: barnie@deakin.edu.au).
 
PDF (278 KB) $25
 Export Citation
 Print
  


Abstract

This paper describes a dual chemiluminescence reagent for the determination of the opiate alkaloids morphine, codeine, oripavine, and thebaine in Papaver somniferum extracts. Detection was achieved using a mixture of acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(ii), where the former acted as both the oxidant for the latter and as a chemiluminescence reagent in its own right. The analytes were separated on a C8 column using ion-pairing HPLC. The application of the mixed reagent detection compared favourably with results obtained using standard HPLC methodology. Detection limits for the alkaloids were 10-6, 5 × 10-7, 3 × 10-6, and 2 × 10-6 mol L-1 for morphine, codeine, oripavine, and thebaine, respectively.

   
Subscriber Login
Username:
Password:  

    


 
Top  Email this page
 
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2012