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Australian Journal of Chemistry
  An international journal for chemical science
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Preliminary Evaluation of Dual Acidic Potassium Permanganate and Tris(2,2′-bipyridyl)ruthenium(ii) Chemiluminescence Detection for the HPLC Determination of Papaver somniferum Alkaloids

Claire E. Lenehan A, Neil W. Barnett A C, Simon W. Lewis A and Kevin M. Essery B

A Centre for Chiral and Molecular Technologies, School of Biological and Chemical Sciences, Deakin University, Geelong VIC 3217, Australia.
B GlaxoSmithKline, Port Fairy VIC 3284, Australia.
C Author to whom correspondence should be addressed (e-mail: barnie@deakin.edu.au).


Abstract

This paper describes a dual chemiluminescence reagent for the determination of the opiate alkaloids morphine, codeine, oripavine, and thebaine in Papaver somniferum extracts. Detection was achieved using a mixture of acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(ii), where the former acted as both the oxidant for the latter and as a chemiluminescence reagent in its own right. The analytes were separated on a C8 column using ion-pairing HPLC. The application of the mixed reagent detection compared favourably with results obtained using standard HPLC methodology. Detection limits for the alkaloids were 10-6, 5 × 10-7, 3 × 10-6, and 2 × 10-6 mol L-1 for morphine, codeine, oripavine, and thebaine, respectively.

Australian Journal of Chemistry 57(10) 1001–1004    doi:10.1071/CH04084
Submitted: 30 March 2004    Accepted: 15 June 2004    Published: 1 October 2004





   
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