Preliminary Evaluation of Dual Acidic Potassium Permanganate and Tris(2,2′-bipyridyl)ruthenium(II) Chemiluminescence Detection for the HPLC Determination of Papaver somniferum Alkaloids

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Contents Vol 57(10)

doi:10.1071/CH04084

Preliminary Evaluation of Dual Acidic Potassium Permanganate and Tris(2,2′-bipyridyl)ruthenium(ii) Chemiluminescence Detection for the HPLC Determination of Papaver somniferum Alkaloids

Claire E. Lenehan ^A, Neil W. Barnett ^A ^C, Simon W. Lewis ^A, Kevin M. Essery ^B

^A Centre for Chiral and Molecular Technologies, School of Biological and Chemical Sciences, Deakin University, Geelong VIC 3217, Australia.
^B GlaxoSmithKline, Port Fairy VIC 3284, Australia.
^C Author to whom correspondence should be addressed (e-mail: barnie@deakin.edu.au).





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Australian Journal of Chemistry 57(10) 1001–1004 http://dx.doi.org/10.1071/CH04084
Submitted: 30 March 2004 Accepted: 15 June 2004 Published online: 1 October 2004

Abstract

This paper describes a dual chemiluminescence reagent for the determination of the opiate alkaloids morphine, codeine, oripavine, and thebaine in Papaver somniferum extracts. Detection was achieved using a mixture of acidic potassium permanganate and tris(2,2′-bipyridyl)ruthenium(ii), where the former acted as both the oxidant for the latter and as a chemiluminescence reagent in its own right. The analytes were separated on a C₈ column using ion-pairing HPLC. The application of the mixed reagent detection compared favourably with results obtained using standard HPLC methodology. Detection limits for the alkaloids were 10^-6, 5 × 10^-7, 3 × 10^-6, and 2 × 10^-6 mol L^-1 for morphine, codeine, oripavine, and thebaine, respectively.