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Synthesis, Characterization, DNA Binding, and Photocleavage of [Ru(bpy)2(MFIP)]2+ and [Ru(phen)2(MFIP)]2+
Lifeng
Tan A B D,
Sheng
Zhang A,
Xiaohua
Liu C,
Yue
Xiao A
A
College of Chemistry, Xiangtan University, Xiangtan 411105, China.
B
Key Lab of Environment-Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan 411105, China.
C
College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
D
Corresponding author. Email: lfwyxh@yahoo.com.cn
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Australian Journal of Chemistry 61(9) 725–731 http://dx.doi.org/10.1071/CH08119
Submitted: 23 March 2008
Accepted: 18 July 2008
Published online: 5 September 2008
Abstract
The new ligand 2-(5-methyl-furan-2-yl)imidazo[4,5-f][1, 10]phenanthroline (MFIP) and its complexes [Ru(bpy)2(MFIP)]2+ 1 (bpy = 2,2′-bipyridine) and [Ru(phen)2(MFIP)]2+ 2 (phen = 1,10-phenanthroline) were synthesized and characterized by elemental analysis, mass spectrometry, and 1H NMR spectroscopy. The DNA binding properties of the two complexes were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that both complexes bind to calf thymus DNA (CT-DNA) through intercalation, and both complexes can enantioselectively interact with CT-DNA. The Λ enantiomers of both complexes are slightly predominant for binding to CT-DNA over the Δ enantiomer. When irradiated at 400 nm, the two complexes promote the photocleavage of pBR322 DNA, and complex 2 cleaves DNA more effectively than complex 1 under comparable experimental conditions. Furthermore, mechanism studies reveal that singlet oxygen (1O2) plays a significant role in the photocleavage.
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