Bacterial Enzyme Responsive Polymersomes: A Closer Look at the Degradation Mechanism of PEG-block-PLA Vesicles
Katrin-Stephanie Tücking A , Stephan Handschuh-Wang A and Holger Schönherr A B
+ Author Affiliations
- Author Affiliations
A Physical Chemistry I, Faculty of Natural Sciences and Engineering, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.
B Corresponding author. Email: schoenherr@chemie.uni-siegen.de
Australian Journal of Chemistry 67(4) 578-584 https://doi.org/10.1071/CH13527
Submitted: 1 October 2013 Accepted: 29 November 2013 Published: 20 January 2014
Abstract
Stimuli-responsive polymersomes that are selectively cleaved by enzymes of pathogenic bacteria are herein reported. The local disruption of the polyester wall in poly(ethylene glycol)-block-poly(lactic acid) (PEG-b-PLA) polymersomes filled with reporter dyes owing to enzymatic degradation by proteinase K was monitored by fluorescence lifetime imaging microscopy. Capsule opening occurred on timescales of minutes to hours, leading to the release of the dye, followed by a slow capsule disintegration, and finally cleavage into monomeric lactic acid over several weeks. These nanocontainers represent a promising element in novel theranostic systems for potential application in advanced wound dressings.
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