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Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Anatomy and essential oils from aerial organs in three species of Aldama (Asteraceae–Heliantheae) that have a difficult delimitation

Aline Bertolosi Bombo A B , Tuane Santos De Oliveira A , Adriana Da Silva Santos De Oliveira C , Vera Lúcia Garcia Rehder C , Mara Angelina Galvão Magenta D and Beatriz Appezzato-Da-Glória A E
+ Author Affiliations
- Author Affiliations

A Departamento de Ciências Biológicas, Escola Superior de Agricultura ‘Luiz de Queiroz’, Universidade de São Paulo, C.P. 09, 13418-900, Piracicaba, SP, Brazil.

B Programa de Pós-graduação em Biologia Vegetal, Instituto de Biologia, UNICAMP, C.P. 6109, 13083-970, Campinas, SP, Brazil.

C Centro de Pesquisas Químicas, Biológicas e Agrícolas, CPQBA, UNICAMP, Paulínia, SP, Brazil.

D Universidade Santa Cecília, Santos, SP, Brazil.

E Corresponding author. Email: bagloria@usp.br

Australian Journal of Botany 60(7) 632-642 https://doi.org/10.1071/BT12160
Submitted: 19 June 2012  Accepted: 3 September 2012   Published: 25 October 2012

Abstract

Recently, molecular analysis caused the South American Viguiera Kunth species to be transferred to Aldama La Llave. However, the circumscription has not been established for certain of the South American species, including Aldama filifolia (Sch.Bip. ex Baker) E.E.Schill. & Panero, A. linearifolia (Chodat) E.E.Schill. & Panero and A. trichophylla (Dusén) Magenta (comb. nov.), which had previously been treated as synonyms because of their high similarity. Therefore, the present study aimed to evaluate the anatomy of the aerial organs, and the yield and chemical composition of the essential oils from these three species, to determine the differences among them and thereby assist in species distinction. The anatomical analysis identified characteristics unique to each species, which are primarily related to the position and occurrence of secretory structures. Histochemical analysis demonstrated that the glandular trichomes and the canals secrete lipophilic substances, which are characterised by the presence of essential oils. The analysis of these essential oils identified monoterpenes as their major constituent and allowed for the recognition of chemical markers for each species. The anatomical and chemical characteristics identified by the present study confirmed that the studied samples belong to three distinct taxa.

Additional keywords: Aldama filifolia, Aldama linearifolia, Aldama trichophylla, glandular trichomes, internode anatomy, leaves, monoterpenes, secretory canals.


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