An unspecific phytotoxin oxalic acid and its effect on sunflower proteome
Maryam Monazzah A , Sattar Tahmasebi Enferadi B C , Mohammad J. Soleimani A and Zohreh Rabiei B
+ Author Affiliations
- Author Affiliations
A Department of Plant Pathology, Faculty of Agriculture, Bu-Ali Sina University, Mahdyeh St., Zip Code: 65174, PO Box 65175–4161, Hamedan, Iran.
B Department of Energy and Environmental biotechnology, Faculty of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran freeway 15 Km, Pajouhesh Boulevard, Zip Code: 1417863171, PO Box 14925/16, Tehran, Iran.
C Corresponding author. Email: tahmasebi@nigeb.ac.ir
Australian Journal of Botany 64(3) 219-226 https://doi.org/10.1071/BT15143
Submitted: 19 June 2015 Accepted: 16 March 2016 Published: 23 May 2016
Abstract
Oxalic acid (OA) is found naturally in many plants and animals: it plays diverse roles in nature. It is an important pathogenicity determinant of many necrotrophic pathogens including Sclerotinia sclerotiorum (Lib.) de Bary. In order to understand the resistance mechanisms in Helianthus annuus L., a proteomic study was conducted on sunflower 12 h after inoculation by OA. A total of 17 differentially expressed proteins (either OA-induced or -suppressed proteins) were identified as a result of OA treatment. The candidate proteins were classified into two groups depending on their up/downregulation. The first group – upregulated proteins – included 13 proteins identified as being involved in the Calvin cycle, photosynthesis, programmed cell death (PCD) pathway, heat shock proteins, proteins with antioxidant activities and flavonol synthase (FLS). The second group – downregulated proteins – included those from the cupin family, ATP synthase subunit β, ketol-acid reducto-isomerase, and actin. Studying the biological significance of proteins responsive to OA might ultimately convey us to improve sunflower lines with higher levels of resistance to Sclerotinia and help to control this devastating necrotrophic plant pathogen.
Additional keywords: Helianthus annuus, hybrid, Sclerotinia sclerotiorum, 2D-electrophoresis.
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