Efficient organogenesis of an Australian passionfruit hybrid (Passiflora edulis x Passiflora edulis var. flavicarpa) suitable for gene delivery
Rhonda M. Hall, Roderick A. Drew, Colleen M. Higgins and Ralf G. Dietzgen
Abstract
An efficient regeneration protocol based on
organogenesis from cotyledon explants and suitable for gene delivery has been
developed for an Australian passionfruit hybrid. Multiple shoots were
regenerated from 30-day-old cotyledon explants on Murashige and Skoog (MS)
medium containing 6-benzylvaminopurine (BAP) and coconut water. Media pulsing
experiments were conducted to investigate the effect on organogenesis of
exposure time of the explants to MS containing 10 _M BAP and 10%
(v/v) coconut water, i.e. passionfruit regeneration medium (PRM).
Continuous exposure of these explants to PRM maximised the number of shoots
produced to 12.1 per explant. However, periods on hormone-free medium improved
the appearance of the shoots and increased the number of explants with shoots
from 75 to 84.6%. Further, shoots exposed for 7 days to half-strength
MS supplemented with 10 mM NAA (1-napthalene acetic acid) produced twice as
many plantlets than those on half-strength MS alone. Transient GUS
histochemical assays indicated delivery of the uidA gene
via Agrobacterium tumefaciens.
Australian Journal of Botany 48(5) 673 - 680 (2000) doi:10.1071/BT99067





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