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RESEARCH ARTICLE

Micro-encapsulated sodium butyrate attenuates oxidative stress induced by corticosterone exposure and modulates apoptosis in intestinal mucosa of broiler chickens

Y. Jiang A B D , W. H. Zhang A D , F. Gao A C and G. H. Zhou A
+ Author Affiliations
- Author Affiliations

A Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Province; College of Animal Science and Technology; Synergetic Innovation Centre of Food Safety and Nutrition, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China.

B Ginling College, Nanjing Normal University, Nanjing 210097, People’s Republic of China.

C Corresponding author. Email: gaofeng0629@sina.com

D These authors contributed equally to this work.

Animal Production Science 55(5) 587-594 https://doi.org/10.1071/AN13348
Submitted: 20 August 2013  Accepted: 7 March 2014   Published: 15 May 2014

Abstract

The aim of the present study was to investigate the effects of micro-encapsulated sodium butyrate (MSB) on oxidative stress and apoptosis induced by dietary corticosterone (CORT) in the intestinal mucosa of broiler chickens. In total, 120 1-day-old male broilers (Arbor Acres) were randomly allocated to two treatment groups and were fed on a control diet (without MSB) or 0.4 g MSB/kg diet. Each treatment had six replicates with five chickens each. From 7 days of age onward, 50% of the chickens in each dietary treatment were subjected to CORT treatment (30 mg/kg of diet). The experimental period was 21 days. The results showed that CORT administration decreased (P < 0.001) feed intake and bodyweight gain and increased (P < 0.001) feed to gain ratio (F : G) of broiler chickens. The dietary MSB supplementation decreased (P < 0.01) F : G and there was an interaction between MSB and CORT on F : G (P < 0.05). Moreover, the activities of superoxide dismutase, glutathione peroxidase and catalase in intestinal mucosa were decreased (P < 0.01 or P < 0.001), and the concentrations of malondialdehyde in the intestinal mucosa were elevated (P < 0.01) by CORT administration. In contrast, treatment of MSB increased (P < 0.01) the catalase activities in duodenal and jejunal mucosa and decreased (P < 0.01) the malondialdehyde concentrations in duodenal mucosa. Higher apoptosis index and lower mRNA expressions of bcl-2 in intestinal epithelial cells were induced (P < 0.05) by CORT treatment. However, MSB decreased (P < 0.05) the apoptosis index and increased the bcl-2 expression. These results suggest that dietary MSB can partially attenuate oxidative stress induced by CORT treatment and inhibit apoptosis of intestinal epithelial cells in broiler chickens.


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