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RESEARCH ARTICLE

Passage kinetics of dry matter and neutral detergent fibre through the gastro-intestinal tract of growing beef heifers fed a high-concentrate diet measured with internal δ13C and external markers

J. B. Daniel A B C , H. Van Laar A B , D. Warner A , J. Dijkstra A , A. Navarro-Villa B and W. F. Pellikaan A
+ Author Affiliations
- Author Affiliations

A Animal Nutrition Group, Wageningen University, PO Box 338, 6700 AH Wageningen, The Netherlands.

B Nutreco R & D, PO Box 220, 5830 AE Boxmeer, The Netherlands.

C Corresponding author. Email: jean-baptiste.daniel@nutreco.com

Animal Production Science 54(9) 1471-1475 https://doi.org/10.1071/AN14354
Submitted: 13 March 2014  Accepted: 11 June 2014   Published: 21 July 2014

Abstract

Fractional rumen passage rates (K1) are fundamental in feed evaluation systems for ruminants to predict the extent of nutrient degradation. Data on passage kinetics of growing beef cattle fed high-concentrate diets are scarce and mainly rely on external passage markers which do not provide nutrient-specific K1 estimates. The present study describes the use of carbon stable isotopes (δ13C) as an internal marker to estimate K1 of dry matter (DM) and neutral detergent fibre (NDF) fractions of a compound feed in a high-concentrate diet, and compares them to the external markers ytterbium (Yb)-actetate and chromium mordanted fibre (Cr-NDF). Four rumen-fistulated Holstein heifers received four times per day a basal diet consisting of barley straw and pelleted compound feed offered separately (ratio 10 : 90, DM basis). Compound feed in the basal diet was mainly based on wheat of low natural 13C enrichment (−28.4 δ13C), which was exchanged with a single dose of a maize-based compound feed of higher natural 13C enrichment (−18.9 δ13C). This difference in natural 13C abundance was used to determine K1 values from faecal 13C excretion patterns. At the same time Yb-Acetate and Cr-NDF were introduced into the rumen to determine K1 values from faecal excretions. Faeces were collected over 90 h after pulse dosing. The K1 of δ13C-marked DM (0.062/h) did not differ (P = 0.745) from δ13C-marked NDF (0.060/h). The δ13C-based K1 values also did not differ from Cr-NDF (0.056/h; P = 0.315). These results indicate similar passage behaviour of these fractions in the rumen of beef heifers fed a high-concentrate diet.

Additional keywords: digestion, feed evaluation, feedlot, rumen.


References

Abrahamse PA, Dijkstra J, Vlaeminck B, Tamminga S (2008a) Frequent allocation of rotationally grazed dairy cows changes grazing behavior and improves productivity. Journal of Dairy Science 91, 2033–2045.
Frequent allocation of rotationally grazed dairy cows changes grazing behavior and improves productivity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXltlWmurg%3D&md5=edfb6f1ec3f19451f86953454ba48e55CAS | 18420633PubMed |

Abrahamse PA, Vlaeminck B, Tamminga S, Dijkstra J (2008b) The effect of silage and concentrate type on intake behavior, rumen function, and milk production in dairy cows in early and late lactation. Journal of Dairy Science 91, 4778–4792.
The effect of silage and concentrate type on intake behavior, rumen function, and milk production in dairy cows in early and late lactation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVKgtrjJ&md5=deacd453e535c02d99a4d19db506b4b1CAS | 19038953PubMed |

Beauchemin KA, Eriksen L, Nørgaard P, Rode LM (2008) Short Communication: salivary secretion during meals in lactating dairy cattle. Journal of Dairy Science 91, 2077–2081.
Short Communication: salivary secretion during meals in lactating dairy cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXltlWmu74%3D&md5=cabe470b00577946c7668577245a92f5CAS | 18420637PubMed |

Bibby J, Toutenburg H (1977) ‘Prediction and improved estimation in linear models.’ (Wiley and Sons: London)

Bruining M, Bosch MW (1992) Ruminal passage rate as affected by CrNDF particle size. Animal Feed Science and Technology 37, 193–200.
Ruminal passage rate as affected by CrNDF particle size.Crossref | GoogleScholarGoogle Scholar |

Colucci PE, MacLeod GK, Grovum WL, McMillan I, Barney DJ (1990) Digesta kinetics in sheep and cattle fed diets with different forage to concentrate ratios at high and low intakes. Journal of Dairy Science 73, 2143–2156.
Digesta kinetics in sheep and cattle fed diets with different forage to concentrate ratios at high and low intakes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3M%2Fjs1ejug%3D%3D&md5=9019eb5441fddefc296cb9a07165d138CAS | 2229603PubMed |

Devant M, Ferret A, Calsamiglia S, Casals R, Gasa J (2001) Effect of nitrogen source in high-concentrate, low-protein beef cattle diets on microbial fermentation studied in vivo and in vitro. Journal of Animal Science 79, 1944–1953.

Dhanoa MS, Siddons RC, France J, Gale DL (1985) A multicompartmental model to describe marker excretion patterns in ruminant faeces. The British Journal of Nutrition 53, 663–671.
A multicompartmental model to describe marker excretion patterns in ruminant faeces.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL28%2FltFCiug%3D%3D&md5=557033e50e85309cc5618e26d63a2c9bCAS | 4063294PubMed |

Ehle FR, Bas F, Barno B, Martin R, Leone F (1984) Particulate rumen turnover rate measurement as influenced by density of passage marker. Journal of Dairy Science 67, 2910–2913.
Particulate rumen turnover rate measurement as influenced by density of passage marker.Crossref | GoogleScholarGoogle Scholar |

Hartnell GF, Satter LD (1979) Determination of rumen fill, retention time and ruminal turnover rates of ingesta at different stages of lactation in dairy cows. Journal of Animal Science 48, 381–392.

Huhtanen P, Hristov AN (2001) Estimating passage kinetics using fibre-bound 15N as an internal marker. Animal Feed Science and Technology 94, 29–41.
Estimating passage kinetics using fibre-bound 15N as an internal marker.Crossref | GoogleScholarGoogle Scholar |

Lee C, Hristov AN (2014) Short communication: comparison of 3 solid digesta passage markers in dairy cows. Journal of Dairy Science 97, 1725–1729.
Short communication: comparison of 3 solid digesta passage markers in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhsVykt7o%3D&md5=7cf437dd2f74e46b5238abe112fee0eeCAS | 24472133PubMed |

Lescoat P, Sauvant D (1995) Development of a mechanistic model for rumen digestion validated using the duodenal flux of amino acids. Reproduction, Nutrition, Development 35, 45–70.
Development of a mechanistic model for rumen digestion validated using the duodenal flux of amino acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXltlertr0%3D&md5=7b48f2cb685826f2d102646b7482cb99CAS | 7873045PubMed |

National Research Council (2001) ‘Nutrient requirements of dairy cattle.’ 7th rev. edn. (National Academy Press: Washington, DC)

Owens FN, Goetsch AL (1986) Digesta passage and microbial protein synthesis. Control of digestion and metabolism in ruminants. In ‘Proceedings of the sixth international symposium on ruminant physiology’. (Eds LP Milligan, WL Grovum, A Dobson) pp. 196–223. (Prentice-Hall: Englewood Cliffs, NJ)

Pellikaan WF, Stringano E, Leenaars J, Bongers DJGM, Schuppen SVLV, Plant J, Mueller-Harvey I (2011) Evaluating effects of tannins on extent and rate of in vitro gas and CH4 production using an automated pressure evaluation system (APES). Animal Feed Science and Technology 166–167, 377–390.
Evaluating effects of tannins on extent and rate of in vitro gas and CH4 production using an automated pressure evaluation system (APES).Crossref | GoogleScholarGoogle Scholar |

Pellikaan WF, Verstegen MWA, Tamminga S, Dijkstra J, Hendriks WH (2013) δ13C as a marker to study digesta passage kinetics in ruminants; a combined in vivo and in vitro study. Animal 7, 754–767.
δ13C as a marker to study digesta passage kinetics in ruminants; a combined in vivo and in vitro study.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXksFKksLw%3D&md5=948c1070fff1acfd84cacbbbab13e466CAS | 23211548PubMed |

Rotger A, Ferret A, Calsamiglia S, Manteca X (2005) Changes in ruminal fermentation and protein degradation in growing Holstein heifers from 80 to 250 kg fed high-concentrate diets with different forage-to-concentrate ratios. Journal of Animal Science 83, 1616–1624.

Seo S, Tedeschi LO, Schwab CG, Lanzas C, Fox DG (2006) Development and evaluation of empirical equations to predict feed passage rate in cattle. Animal Feed Science and Technology 128, 67–83.
Development and evaluation of empirical equations to predict feed passage rate in cattle.Crossref | GoogleScholarGoogle Scholar |

Siddons RC, Paradine J, Beever DE, Cornell PR (1985) Ytterbium acetate as a particulate-phase digesta-flow marker. The British Journal of Nutrition 54, 509–519.
Ytterbium acetate as a particulate-phase digesta-flow marker.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXlsVSrsro%3D&md5=e7d5ed15b7108e0613e4b32a81b772f9CAS | 2998454PubMed |

Sterk A, Vlaeminck B, van Vuuren AM, Hendriks WH, Dijkstra J (2012) Effects of feeding different linseed sources on omasal fatty acid flows and fatty acid profiles of plasma and milk fat in lactating dairy cows. Journal of Dairy Science 95, 3149–3165.
Effects of feeding different linseed sources on omasal fatty acid flows and fatty acid profiles of plasma and milk fat in lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XntlymtbY%3D&md5=a9107f5ada27e12a08aad8bfcb78466bCAS | 22612951PubMed |

Südekum KH, Ziggers W, Roos N, Sick H, Tamminga S, Stangassinger M (1995) Estimating the passage of digesta in steers and wethers using the ratio of 13C to 12C and titanium (IV) oxide. Isotopes in Environmental and Health Studies 31, 219–227.
Estimating the passage of digesta in steers and wethers using the ratio of 13C to 12C and titanium (IV) oxide.Crossref | GoogleScholarGoogle Scholar |

Udén P, Colucci PE, Van Soest PJ (1980) Investigation of chromium, cerium and cobalt as markers in digesta. Journal of the Science of Food and Agriculture 31, 625–632.
Investigation of chromium, cerium and cobalt as markers in digesta.Crossref | GoogleScholarGoogle Scholar | 6779056PubMed |

Warner D, Dijkstra J, Tamminga S, Pellikaan WF (2013a) Passage kinetics of concentrate in dairy cows measured with carbon stable isotopes. Animal 7, 1935–1943.
Passage kinetics of concentrate in dairy cows measured with carbon stable isotopes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsl2htL%2FK&md5=369930667bcbbdd055223dc4f77acdf2CAS | 24016555PubMed |

Warner D, Dijkstra J, Hendriks WH, Pellikaan WF (2013b) Passage kinetics of 13C-labeled corn silage components through the gastrointestinal tract of dairy cows. Journal of Dairy Science 96, 5844–5858.
Passage kinetics of 13C-labeled corn silage components through the gastrointestinal tract of dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVKnu7jI&md5=6dd517226320b60bbe1db65d1900cf3cCAS | 23831103PubMed |

Warner D, Dijkstra J, Hendriks WH, Pellikaan WF (2014) Stable isotope-labelled feed nutrients to assess nutrient-specific feed passage kinetics in ruminants. Journal of the Science of Food and Agriculture 94, 819–824.
Stable isotope-labelled feed nutrients to assess nutrient-specific feed passage kinetics in ruminants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhslWjtbrK&md5=837ae851f9dba164811495ceb61636f6CAS | 24114801PubMed |