Birdsfoot trefoil (Lotus corniculatus L.) is a perennial pasture legume with adaptive characteristics and agronomic potential for permanent pastures in the high rainfall temperate zone in eastern Australia. Most birdsfoot trefoil cultivars have been developed for high latitude (>40° north or south) locations, and have long photoperiod (>16 h) requirements for flowering. However, at low latitude (28–32°S) in northern New South Wales (NSW), daylength (~14 h on the summer solstice) provides insufficient photoperiod to stimulate flowering and the level of seed-set needed for effective seedling recruitment. As a precursor to breeding, a study was undertaken to characterise a collection of birdsfoot trefoil populations from low latitude origins. Eight cultivars, two breeding lines and 38 accessions were assessed for flowering prolificacy, seed-set, morphological characteristics and seasonal herbage growth at two sites in northern NSW (Glen Innes 29°42′S, Armidale 30°31′S). None of the cultivars expressed reproductive processes with sufficient intensity to provide the level of seed-set necessary for regeneration. However, three accessions consistently flowered strongly at both sites and expressed desirable agronomic characteristics, and a further 10 accessions and the Australian germplasm BL_VR5 flowered strongly at least at one of two sites. These populations provide a genetic base for future cultivar development, so that birdsfoot trefoil might become a perennial legume option for northern NSW and other low latitude regions.