A Hologic QDR4500W dual energy X-ray absorptiometer (DXA) was used to measure body composition in 60 sheep half carcasses ranging from 8 to 28 kg. Half carcasses were from ewes and wethers of mixed genetics. Values determined by DXA, including total tissue mass (TTM), lean tissue mass (LTM), fat tissue mass (FTM) and bone mineral content (BMC), for the half carcass were evaluated by comparison with chemically determined composition. In the case of BMC, the relationship was with chemically determined ash content. Liveweight and chemically determined lean, fat and ash were strongly related to DXA-derived values for TTM, LTM, FTM and BMC, respectively (R² = 0.999, 0.986, 0.989 and 0.920, respectively). However, because DXA estimates were different from chemically determined values in this sample of carcasses, they needed to be adjusted with the use of appropriate regression equations to correct the in-built algorithms. These data demonstrate the efficacy of DXA as a non-destructive method for determining the composition of the sheep half carcass.