The oestrogenic mycotoxin zearalenone can occur in pasture and fodder infected with various Fusarium spp., and at concentrations exceeding 1 mg/kg, it has been associated with a reduction in the fertility of grazing ruminants. Pasture and fodder samples collected in four small studies from cattle and sheep farms in south-eastern Australia were tested for zearalenone and other mycotoxins using high performance liquid chromatography. Zearalenone in winter pasture was detected (viz. >0.1 and up to 5.0 mg/kg DM) in 8 of 22 pastures; three exceeded the tolerance concentration, 1.0 mg/kg. Zearalenone was detected in 15 of 24 samples of silage that were submitted by farmers for nutritional assessment, but that were not suspected of toxicity; 12 were in the range 1.0–80 mg/kg. Zearalenone was correlated with crude protein concentration (r = 0.59, P < 0.05); it was high in legume-dominant compared with grass-dominant silage (P < 0.04). In a separate investigation of 28 feed samples submitted for mycotoxin screening by nutritionists/veterinarians, zearalenone was detected in 7 of 13 pastures, in two of two hays and 9 of 13 silages (6 of 28 exceeded 1 mg/kg). Deoxynivalenol was detected in 6 of 13 pastures, one of two hays and 10 of 13 silages. Deoxynivalenol was high (0.64–1.76 mg/kg) in five silages of excellent/average appearance. Ergovaline and lolitrem B were detected at above tolerable concentrations in four of eight perennial ryegrass-dominant samples examined. Aflatoxins, ochratoxin A and fumonisins were detected in some samples although the concentrations were low. Two samples contained either zearalenone and ergovaline, or zearalenone and deoxynivalenol, where both mycotoxins exceeded tolerable concentrations.