Understanding the process by which nutrients and solids enter waterways from pastures in the Great Lakes district, New South Wales, Australia, may assist in maintaining water quality to ensure ongoing environmental and economic sustainability of the region. Rainfall simulations, using a 100-year return storm event, were conducted to determine nutrient and suspended solid concentrations in the runoff of 8 pasture sites in 3 of the catchments in the district. On 5 of the 8 sites, considerable concentrations of N or P were mobilised during the simulated rainfall event, but average nutrient concentrations and total loads across all sites were relatively low and similar to other studies of nutrient runoff from pastures. In addition, low runoff coefficients indicated that runoff is probably not the major pathway for nutrient losses from pasture in this area. Overall, rainfall runoff responses at the sites were similar in the 3 catchments. In contrast, the results suggest that, despite generating more runoff, the sites in the Wang Wauk catchment generated less nutrients in runoff than the sites in the Wallamba and Myall catchments. There was no difference in total suspended solids loads for the sites analysed by catchment. Relationships between soil physical and chemical characteristics and total nutrients loads or cumulative runoff were not strong.