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Australian Journal of Zoology Australian Journal of Zoology Society
Evolutionary, molecular and comparative zoology
RESEARCH ARTICLE

Isolation and characterisation of 14 polymorphic microsatellite loci for the plant-louse Trioza barrettae (Hemiptera : Triozidae)

Melinda L. Moir A , Yvette Hitchen B , Michael G. Gardner C , David J. Coates D and W. Jason Kennington E F
+ Author Affiliations
- Author Affiliations

A School of Plant Biology, Ecosystem Restoration and Intervention Ecology Research Group, The University of Western Australia, Crawley, WA 6009, Australia.

B Helix Molecular Solutions, PO Box 155, Leederville, WA 6903, Australia.

C School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia; Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA 5005, Australia; and Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia.

D Western Australian Department of Parks and Wildlife, Locked Bag 104, Bentley Delivery Centre, WA 6983, Australia.

E Centre for Evolutionary Biology, School of Animal Biology, The University of Western Australia, Crawley, WA 6009, Australia.

F Corresponding author. Email: jason.kennington@uwa.edu.au

Australian Journal of Zoology 63(3) 201-203 https://doi.org/10.1071/ZO15009
Submitted: 25 February 2015  Accepted: 12 July 2015   Published: 30 July 2015

Abstract

We describe the isolation and development of 14 polymorphic microsatellite loci for the critically endangered plant-louse Trioza barrettae (Hemiptera : Triozidae). The loci were tested in 25 individuals from a single population situated in the south-west of Western Australia. No evidence of linkage disequilibrium was detected between any pair of loci. However, eight loci showed significant departures from Hardy–Weinberg expectations. The number of alleles per locus ranged from 2 to 27.

Additional keywords: lerp insects, microsatellites, Psylloidea.


References

Barrett, S., Shearer, B. L., Crane, C. E., and Cochrane, A. (2008). An extinction-risk assessment tool for flora threatened by Phytophthora cinnamomi. Australian Journal of Botany 56, 477–486.
An extinction-risk assessment tool for flora threatened by Phytophthora cinnamomi.Crossref | GoogleScholarGoogle Scholar |

Coates, D. J., and McArthur, S. (2009). Assessing the taxonomic status of Banksia brownii and patterns of genetic diversity in extinct and extant populations. Bankwest Landscope Project. Western Australian Department of Environment and Conservation, Perth.

Gardner, M. G., Fitch, A. J., Bertozzi, T., and Lowe, A. J. (2011). Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development. Molecular Ecology Resources 11, 1093–1101.
Rise of the machines – recommendations for ecologists when using next generation sequencing for microsatellite development.Crossref | GoogleScholarGoogle Scholar | 21679314PubMed |

Kalinowski, S., Taper, M., and Marshall, T. (2007). Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology 16, 1099–1106.
Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment.Crossref | GoogleScholarGoogle Scholar | 17305863PubMed |

Meglécz, E., Costedoat, C., Dubut, V., Gilles, A., Malausa, T., Pech, N., and Martin, J.-F. (2010). QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26, 403–404.
QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects.Crossref | GoogleScholarGoogle Scholar | 20007741PubMed |

Raymond, M., and Rousset, F. (1995). GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. The Journal of Heredity 86, 248–249.

Rice, W. R. (1989). Analyzing tables of statistical tests. Evolution 43, 223–225.
Analyzing tables of statistical tests.Crossref | GoogleScholarGoogle Scholar |

Taylor, G. S., and Moir, M. L. (2014). Further evidence of the coextinction threat for jumping plant-lice: three new Acizzia (Psyllidae) and Trioza (Triozidae) from Western Australia. Insect Systematics & Evolution 45, 283–302.
Further evidence of the coextinction threat for jumping plant-lice: three new Acizzia (Psyllidae) and Trioza (Triozidae) from Western Australia.Crossref | GoogleScholarGoogle Scholar |