Segregation of glutenins in wheat × maize-derived doubled haploid wheat populations

Stephen J. Kammholz, Raechelle A. Grams, Phillip M. Banks and Mark W. Sutherland

Abstract

The segregation of both high and low molecular weight glutenin subunits across 7 F₁ wheat (Triticum aestivum L.) × maize (Zea mays L.) derived doubled haploid populations was examined. The F₁ wheats used in each population were produced from parents of interest to Australian wheat breeding programs. The parents varied by up to 5 glutenin subunit loci. Examination of subunits individually within each population using a chi-square analysis revealed that all but 2 of the 26 pairs of alleles analysed fitted the expected 1 : 1 segregation ratio. Glutenin profiles were examined for each cross individually and all but one (Sonalika/Hartog) fitted the expected Mendelian segregation pattern. The analysis of allele distribution of the 6 glutenin loci across all 7 crosses showed all falling well within expected segregation ratios. Closer examination of parental lines and populations revealed irregularities which conflict with original assumptions and provide a valid explanation for the few segregation distortions observed. It is concluded that wheat × maize-derived doubled haploid populations represent a unbiased assortment of parental gametes on both arms of Group 1 chromosomes.

Keywords: Triticum aestivum, Zea mays, glutenin subunits, segregation distortion.

Australian Journal of Agricultural Research 49(8) 1253 - 1260

Full text doi:10.1071/A98032

© CSIRO 1998