Liquid-phase denaturant gradient gel electrophoresis profiles of rumen bacteria from Brahman cross steers selected into two groups on the basis of post-weaning liveweight gain on low crude protein pasture

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Contents Vol 52(7)

doi:10.1071/AN11234

Food, Fibre and Pharmaceuticals from Animals

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Liquid-phase denaturant gradient gel electrophoresis profiles of rumen bacteria from Brahman cross steers selected into two groups on the basis of post-weaning liveweight gain on low crude protein pasture

E. D. Martínez ^A ^B ^D, K. E. Turnbull ^A, S. P. Quigley ^A, S. J. Streeter ^C, A. Swain ^B, A. V. Klieve ^A ^B, D. Ouwerkerk ^B and D. P. Poppi ^A

^A School of Agriculture and Food Science, University of Queensland, Gatton, Qld 4343, Australia.
^B Agri-Science Queensland, EcoScience Precinct, Dutton Park, Qld 4102, Australia.
^C Northern Territory Department of Resources, Katherine, NT 0851, Australia.
^D Corresponding author. Email: emilio.martinezgarbarino@uqconnect.edu.au

Animal Production Science 52(7) 647-652 http://dx.doi.org/10.1071/AN11234
Submitted: 18 November 2011 Accepted: 16 March 2012 Published: 24 April 2012





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Abstract

Two experiments were conducted to evaluate the relationship between rumen liquid-associated bacterial community structures and post-weaning liveweight gain (LWG) of Brahman crossbred steers. Bacterial diversity was assessed using denaturant gradient gel electrophoresis (DGGE). In Experiment 1, 16 steers were selected from a group of 100 steers by pairing steers with the same weaning weight, but different LWG 90 days after weaning (n = 8 highest growth, 0.21 ± 0.01 kg/day; and n = 8 lowest growth –0.07 ± 0.01 kg/day). Thereafter, steers were allocated to a 28-day pen study and fed Mitchell grass (Astrebla spp.) hay to examine DM intake and digestibility, rumen parameters and rumen microbial community in these two groups. Rumen fluid samples were taken by stomach tube 3 h after feeding on the last day of the pen phase. In Experiment 2, 12 pairs of weaned steers were selected from a group of 203 steers on the same basis as Experiment 1. The post-weaning LWG were 0.20 ± 0.03 and 0.02 ± 0.03 kg/day for the 12 highest and 12 lowest growth animals selected, respectively. Steers then grazed dry season Sabi grass (Urochloa mosambicensis) dominant pasture for 21 days, before rumen sampling 3 h after morning grazing by stomach tubing on the last day. In Experiment 1, there were no significant differences between the two groups in DM intake, digestibility, ruminal pH, total volatile fatty acid (VFA) concentration or the VFA proportion. In Experiments 1 and 2, rumen ammonia-N concentration was similar between LWG groups and there was no evidence of a relationship between liquid-phase DGGE profiles of rumen bacteria and high or low post-weaning LWG using multivariate analyses. Furthermore, the number of detected DGGE bands, the Shannon–Wiener and evenness indexes were not different between LWG groups. This DGGE analysis of the most abundant groups of rumen fluid-associated bacteria suggests that microbial populations were not related with the differences observed in post-weaning LWG within a group of weaners fed low crude protein diets.

References

Arthur PF, Archer JA, Herd RM (2004) Feed intake and efficiency in beef cattle: overview of recent Australian research and challenges for the future. Australian Journal of Experimental Agriculture 44, 361–369.
| CrossRef |

Clarke AM, Drennan MJ, McGee M, Kenny DA, Evans RD, Berry DP (2009) Live animal measurements, carcass composition and plasma hormone and metabolite concentrations in male progeny of sires differing in genetic merit for beef production. Animal 3, 933–945.
| CrossRef | CAS |

CSIRO (2007) ‘Nutrient requirements of domesticated ruminants.’ (CSIRO Publishing: Melbourne)

Edwards JE, Huws SA, Kim EJ, Lee MRF, Kingston-Smith AH, Scollan ND (2008) Advances in microbial ecosystem concepts and their consequences for ruminant agriculture. Animal 2, 653–660.
| CrossRef | CAS |

Firkins JL, Karnati SKR, Yu Z (2008) Linking rumen function to animal response by application of metagenomics techniques. Australian Journal of Experimental Agriculture 48, 711–721.
| CrossRef |

Green SJ, Leigh MB, Neufeld JD (2010) Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis. In ‘Handbook of hydrocarbon and lipid microbiology’. (Ed. KN Timmis) pp. 4137–4158. (Springer-Verlag: Berlin)

Guan LL, Nkrumah JD, Basarab JA, Moore SS (2008) Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle’s feed efficiency. FEMS Microbiology Letters 288, 85–91.
| CrossRef | CAS |

Hegarty RS (2004) Genotype differences and their impact on digestive tract function of ruminants: a review. Australian Journal of Experimental Agriculture 44, 459–467.
| CrossRef |

Herd RM, Arthur PF (2009) Physiological basis for residual feed intake. Journal of Animal Science 87, E64–E71.
| CrossRef | CAS |

Hernandez-Sanabria E, Guan LL, Goonewardene LA, Li M, Mujibi DF, Stothard P, Moore SS, Leon-Quintero MC (2010) Correlation of particular bacterial PCR-denaturing gradient gel electrophoresis patterns with bovine ruminal fermentation parameters and feed efficiency traits. Applied and Environmental Microbiology 76, 6338–6350.
| CrossRef | CAS |

Hernandez-Sanabria E, Goonewardene LA, Wang Z, Durunna ON, Moore SS, Guan LL (2012) Impact of feed efficiency and diet on adaptive variations in the bacterial community in the rumen fluid of cattle. Applied and Environmental Microbiology 78, 1203–1214.
| CrossRef | CAS |

Klieve AV, O’Leary MN, McMillen L, Ouwerkerk D (2007) Ruminococcus bromii, identification and isolation as a dominant community member in the rumen of cattle fed a barley diet. Journal of Applied Microbiology 103, 2065–2073.
| CrossRef | CAS |

Kocherginskaya SA, Cann IK, Mackie RI (2005) Denaturing gradient gel electrophoresis. In ‘Methods in gut microbial ecology for ruminants’. (Eds HPS Makkar, CS McSweeney) pp. 119–127. (Springer: Dordrecht, The Netherlands)

Mackie RI, McSweeney CS, Aminov R (2001) Rumen. In ‘eLS’. (John Wiley & Sons Ltd: Chichester, UK) Available at www.els.net. 10.1038/npg.els.0000404

Muyzer G, Smalla K (1998) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoek 73, 127–141.
| CrossRef | CAS |

Muyzer G, Dewaal EC, Uitterlinden AG (1993) Profiling of complex microbial-populations by denaturing gradient gel-electrophoresis analysis of polymerase chain reaction-amplified genes-coding for 16s ribosomal-RNA. Applied and Environmental Microbiology 59, 695–700.
| CAS |

Oddy VH, Harper GS, Greenwood PL, McDonagh MB (2001) Nutritional and developmental effects on the intrinsic properties of muscles as they relate to the eating quality of beef. Australian Journal of Experimental Agriculture 41, 921–942.
| CrossRef | CAS |

Pitta D, Pinchak W, Dowd SE, Osterstock J, Gontcharova V, Youn E, Dorton K, Yoon I, Min BR, Fulford JD, Wickersham TA, Mallinowski DP (2010) Rumen bacterial diversity dynamics associated with changing from Bermudagrass hay to grazed winter wheat diets. Microbial Ecology 59, 511–522.
| CrossRef |

Spellerberg IF (2008) Shannon–Wiener index. In ‘Encyclopedia of ecology’. (Eds SE Jorgensen, B Fath) pp. 3249–3252. (Academic Press: Oxford)

Turnbull KE, Quigley SP, Kidd JF, McLennan SR, Poppi DP (2008) Differences in utilization of low-quality hay between cattle selected on growth rate after weaning. In ‘Proceeding of the Australian Society of Animal Production. Vol. 27’. pp. 53. (The Australian Society of Animal Production)

Weimer PJ, Waghorn GC, Odt CL, Mertens DR (1999) Effect of diet on populations of three species of ruminal cellulolytic bacteria in lactating dairy cows. Journal of Dairy Science 82, 122–134.
| CrossRef | CAS |

Winter WH, Ninks L, Seebeck RM (1991) Sustaining productive pastures in the tropics. 10. Forage and feeding systems for cattle. Tropical Grasslands 25, 145–152.

Yu Z, Forster RJ (2005) Nucleic acid extraction, oligonucleotide probes and PCR methods. In ‘Methods in gut microbial ecology for ruminants’. (Eds HPS Makkar, CS McSweeney) pp. 81–104. (Springer: Dordrecht, The Netherlands)

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